Method for preparing yeast culture from distiller's grains and application of yeast culture
A technology of yeast culture and distiller's grains, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of low added value, poor taste, and insufficient use of nutritional value, etc., to reduce production costs , the effect of saving resources
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Embodiment 1
[0039] Preparation of dried distiller's grains: drying the fresh distiller's distiller's grains shipped from the distillery at 115°C with a dryer until the water content is lower than 15%, to obtain dried distiller's grains.
[0040] Preparation of Saccharomyces cerevisiae liquid: first activate with YPD slant medium at 30°C for 20h, then use YPD fermentation medium to ferment at 30°C and 180r / min for 5 days.
[0041] YPD slant medium includes the following components by weight: 3 parts of yeast extract, 4 parts of peptone, 4 parts of glucose and 1000 mL of distilled water;
[0042] The YPD fermentation medium comprises the following components in parts by weight: 2 parts of yeast extract, 3 parts of peptone, 3 parts of glucose and 1000 mL of distilled water.
[0043] Preparation of Aspergillus niger liquid: first activate with YPD slant medium at 30°C for 20h, and then use YPD fermentation medium for fermentation at 30°C and 180r / min for 4 days.
[0044] YPD slant medium inclu...
Embodiment 2
[0053] The method for using distiller's grains to ferment yeast culture comprises the following steps:
[0054] Take 200 kg of dried distiller's grains in Example 1, mix the dried distiller's grains with corn flour at a weight ratio of 1:0.2, and mix the dried distiller's grains with corn flour at a material-to-water ratio of 1:1 based on the total amount of dried distiller's grains and corn flour. The mixed material is stirred and mixed with water, and the pH is adjusted to be about 5.0 to obtain the pretreated distiller's grains;
[0055] According to the total amount of dry basis of the pretreated distiller's grains, insert 5% of the mixed bacteria liquid (in the embodiment 1, the brewing yeast liquid, the lactic acid bacteria liquid and the Aspergillus niger liquid are mixed in a volume ratio of 1:1:1), and add Mix 1% enzyme solution (liquefied enzyme solution, glucoamylase solution, phytase solution, and protease solution mixed at a volume ratio of 1:0.4:0.1:0.2) to obtai...
Embodiment 3
[0059] The method for using distiller's grains to ferment yeast culture comprises the following steps:
[0060] The difference from Example 2 is that the dry distiller's grains and corn flour are mixed in a weight ratio of 1:0.3, and the rest of the steps are the same.
[0061] Above-mentioned, before inoculating Bacillus subtilis after fermentation finishes, adopt PCA counting method, measure the yeast number of gained yeast culture to be 1.93 * 10 9 cfu / g; total protein 16.7%.
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