Method for preparing yeast culture from distiller's grains and application of yeast culture

A technology of yeast culture and distiller's grains, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of low added value, poor taste, and insufficient use of nutritional value, etc., to reduce production costs , the effect of saving resources

Active Publication Date: 2021-02-09
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, feeding cows or other animals directly with distiller's grains not only does not fully utilize the nutritional value, but also has poor taste
In addition, distiller's grains also contain a large amount of cellulose, aroma substances, etc. At present, distiller's grains are mainly used as feed, making seasonings and cultivating edible fungi, etc., with low added value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of dried distiller's grains: drying the fresh distiller's distiller's grains shipped from the distillery at 115°C with a dryer until the water content is lower than 15%, to obtain dried distiller's grains.

[0040] Preparation of Saccharomyces cerevisiae liquid: first activate with YPD slant medium at 30°C for 20h, then use YPD fermentation medium to ferment at 30°C and 180r / min for 5 days.

[0041] YPD slant medium includes the following components by weight: 3 parts of yeast extract, 4 parts of peptone, 4 parts of glucose and 1000 mL of distilled water;

[0042] The YPD fermentation medium comprises the following components in parts by weight: 2 parts of yeast extract, 3 parts of peptone, 3 parts of glucose and 1000 mL of distilled water.

[0043] Preparation of Aspergillus niger liquid: first activate with YPD slant medium at 30°C for 20h, and then use YPD fermentation medium for fermentation at 30°C and 180r / min for 4 days.

[0044] YPD slant medium inclu...

Embodiment 2

[0053] The method for using distiller's grains to ferment yeast culture comprises the following steps:

[0054] Take 200 kg of dried distiller's grains in Example 1, mix the dried distiller's grains with corn flour at a weight ratio of 1:0.2, and mix the dried distiller's grains with corn flour at a material-to-water ratio of 1:1 based on the total amount of dried distiller's grains and corn flour. The mixed material is stirred and mixed with water, and the pH is adjusted to be about 5.0 to obtain the pretreated distiller's grains;

[0055] According to the total amount of dry basis of the pretreated distiller's grains, insert 5% of the mixed bacteria liquid (in the embodiment 1, the brewing yeast liquid, the lactic acid bacteria liquid and the Aspergillus niger liquid are mixed in a volume ratio of 1:1:1), and add Mix 1% enzyme solution (liquefied enzyme solution, glucoamylase solution, phytase solution, and protease solution mixed at a volume ratio of 1:0.4:0.1:0.2) to obtai...

Embodiment 3

[0059] The method for using distiller's grains to ferment yeast culture comprises the following steps:

[0060] The difference from Example 2 is that the dry distiller's grains and corn flour are mixed in a weight ratio of 1:0.3, and the rest of the steps are the same.

[0061] Above-mentioned, before inoculating Bacillus subtilis after fermentation finishes, adopt PCA counting method, measure the yeast number of gained yeast culture to be 1.93 * 10 9 cfu / g; total protein 16.7%.

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Abstract

The invention discloses a method for preparing a yeast culture through distillers' grains and an application of the yeast culture. The method comprises the following steps of S1, supplementing a carbon source to distillers' grains to obtain pretreated distillers' grains, inoculating mixed bacterium liquid to the pretreated distillers' grains, adding mixed enzyme liquid, preparing a fermentation base material, and performing fermentation culture for 96-120h; and S2, after fermentation is completed, performing inoculation with bacillus subtilis, continuing to perform fermentation culture for 24h, and performing baking, crushing and screening so as to obtain the yeast culture, wherein the mixed bacterium liquid comprises 3 strains of saccharomyces cerevisia, lactic acid bacteria and aspergillus niger, and the mixed enzyme liquid comprises liquefying amylase, glucoamylase, phytase and proteases. According to the method disclosed by the invention, the distillers' grains are used as substrates, the mixed bacterium liquid and the mixed enzyme liquid are added for heap fermentation, so that the yeast culture is obtained; and the distillers' grains are turned into wealth from waste, and besides, a feed rich in nutrient components is provided for animals, so that improvement of the immunity of the animals can be facilitated.

Description

technical field [0001] The invention relates to the technical field of animal feed, in particular to a method for preparing yeast culture from distiller's grains and the application of the yeast culture. Background technique [0002] Yeast culture refers to complex fermentation products obtained by deep fermentation of yeast in a specific medium under certain process conditions, including cell metabolites (including polypeptides, organic acids, amino acids, nucleic acids, enzymes, unknown growth factors, etc.) , denatured medium (containing oligosaccharides, polypeptides, etc.) and yeast cells themselves (proteins, amino acids, nucleic acids, cell wall polysaccharides, etc.), rich in nutrients, is a new type of green microecological preparation. Yeast culture can promote the growth of animal gastrointestinal microorganisms, improve digestion, enhance immunity, and detoxify, so it is beneficial to the healthy growth of animals and has broad application prospects in livestock ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/14A23K10/38A23K10/30A23K50/30A23K50/75C12N1/20C12N1/18C12N1/14C12R1/685C12R1/865
CPCA23K10/12A23K10/14A23K10/30A23K10/38A23K50/30A23K50/75C12N1/14C12N1/18C12N1/20Y02P60/87
Inventor 彭楠卢立轩常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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