Selenium-rich cordyceps culturing method

A cultivation method and technology of Cordyceps militaris, which is applied in the field of cultivation of selenium-enriched Cordyceps militaris, can solve problems such as difficulty in improving the nutritional and medicinal value of Cordyceps militaris, difficulty in improving the nutritional value of Cordyceps militaris, etc., and achieve bright color, strong growth potential, and improvement of nutritional and medicinal value. Effect

Inactive Publication Date: 2010-12-01
YIWU DANXI MEDECINAL BIOLOGY DEV RESINST +1
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Problems solved by technology

Selenium is an essential limited element for the human body. It has various pharmacological effects such as anti-cancer, heart protection, anti-hepatic necrosis, prevention and treatment of myopia and cataract, detoxification, improving immunity, delaying aging and enhancing reproductive function. Therefore, the development of selenium-enriched fungi should be Causing people's attention, cultivating selenium-enriched Cordyceps militaris can improve the nutrition and medicinal value of Cordyceps militaris, for this reason, people have proposed again open date: February 21st, 2001, publication number: 1284559, patent application number: 99111376 " Selenium Enrichment and Its Trace Element Cordyceps", Publication Date: November 15, 2006, Publication Number: CN1861782A, Patent Application Number: 20061004667.3 "Artificial Cultivation Method of Selenium Enriched Cordyceps and Application of Fruiting Bodies" Technology for Cultivating Selenium Enriched Cordyceps militaris scheme, but what selenium all adopted in the above-mentioned technical scheme is the nutrient solution containing selenium fertilizer, and the nutrient solution of selenium fertilizer all adopts sodium selenite as the source of selenium element, adopts sodium selenite as the source of selenium element Cordyceps is difficult to absorb, so The Cordyceps cultivated by the above method has extremely low selenium content, and it is difficult to improve the nutritional and medicinal value of Cordyceps

Method used

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example

[0015] Below is an example of the present invention, comprises following flow process step:

[0016] (1) carry out the activation of bacterial classification in PDA solid medium, the excellent bacterial classification preserved in the refrigerator, once every two months is activated on PDA solid medium; Take 200g of potatoes from the filtrate, 20g of glucose, KH 2 PO 4 2g, 15g agar, 970ml water;

[0017] (2), the cultivation of liquid bacterial classification: through the bacterial classification that activates in PDA solid medium, be inoculated into liquid medium and cultivate, culture time 2~4 days, the pH of liquid medium is adjusted to 6.0; Formula by weight ratio: glucose 20g, peptone 5g, yeast powder 5g, KH 2 PO 4 0.04g, NaCl 5g, MgSO 4 0.04g, vitamin B 1 0.02g, water 950ml;

[0018] It also performs:

[0019] (3), Selenium-enriched cultivation of fruiting bodies

[0020] a, the formula of fruiting body selenium-enriched medium is as follows: it is by the en...

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Abstract

The invention relates to a selenium-rich cordyceps culturing method belonging to the technical field of cordyceps culturing methods. The selenium-rich cordyceps culturing method comprises the following process steps of: (1) activating strains in a PDA (Potato Dextrose Agar) solid culture medium; and (2) culturing liquid strains. The selenium-rich cordyceps culturing method is characterized by also comprising the following step of: (3) carrying out sporocarp selenium-rich culture, wherein a sporocarp selenium-rich culture medium is formed by rice, selenate and a glutelin hydrolyzing solution, and the selenate is one of sodium selenate, potassium selenate and ammonium selenate; then inoculating the liquid strains cultured by a fermentation liquid medium into the sporocarp selenium-rich culture medium with the formula, and culturing for 40-50 days according to fermentation management requirements. The cultured cordyceps can greatly increase the content of selenium contained in cordyceps sporocarps to 3.83-81.95Mug / g; the cordyceps sporocarps have high growth vigour, higher output and bright colors, therefore the nutritive and medical value of the cordyceps is enhanced; and besides, the invention has implementing conditions which are easy to achieve, thereby being popularized in a whole industry.

Description

technical field [0001] The invention belongs to the technical field of methods for cultivating Cordyceps militaris, in particular to a method for cultivating selenium-enriched Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the fungus Ascomycetes and Cordyceps in taxonomy. Cordycepin is a 3′-deoxyadenosine nucleoside isolated from the culture medium of Cordyceps militaris in 1950. It has significant pharmacological effects, such as antibacterial effect, anti-tumor effect, anti-virus effect, and enhanced immunity. Function, prevention and treatment of cardiovascular and cerebrovascular diseases, etc. Modern scientific research shows that cordycepin is an important active ingredient of Cordyceps militaris, the content of cordycepin in Cordyceps militaris is dozens of times that of Cordyceps sinensis, and it is the main source of cordycepin. Cordyceps militaris has the effects of anti-fatigue, anti-tumor, h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 王宪俊朱丹华
Owner YIWU DANXI MEDECINAL BIOLOGY DEV RESINST
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