A production method for improving the umami taste of kelp juice
A production method and kelp technology are applied in the production field of improving the umami taste of kelp juice, can solve the problems of limited improvement of umami taste of kelp juice, short shelf life of umami, detrimental to physical health, etc., and achieve fast growth and reproduction, generation time Short, freshness-enhancing effect
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Embodiment 1
[0030] A production method for improving the umami taste of kelp juice, comprising the steps of:
[0031] 1) Add 20kg of dried kelp into the extraction tank, inject 500L of demineralized water, soak for 30 minutes, raise the temperature to 75°C, and extract for 2 hours;
[0032] 2) Pump the collected 340L extract into the expansion tank, add 15Kg glucose and 1.5Kg glycine into the expansion tank, and stir evenly;
[0033] 3) Sterilize at 100°C for 10 minutes, then cool down to 30°C;
[0034] 4) Add 9L of activated Candida utilis culture medium (OD value 0.792) into the sterilized extract at 30°C for expansion. The expansion temperature is 28°C and the ventilation rate is 12L / min, the expansion time is 12 hours; the protein content in the kelp juice is 22.7g / L;
[0035] 5) After the expansion, heat the expansion solution to 50°C, add 90g of flavor protease, and enzymolyze it for 2 hours at 50°C, then use a high-frequency ultrasonic wall breaker to treat the kelp juice twice,...
Embodiment 2
[0039] A production method for improving the umami taste of kelp juice, comprising the steps of:
[0040] 1) Add 50kg of dried kelp into the extraction tank, inject 1000L of demineralized water, soak for 30 minutes, raise the temperature to 75°C, and extract for 2 hours;
[0041] 2) Pump the collected 600L extract into the expansion tank, add 40Kg glucose and 4Kg glycine into the expansion tank, and stir evenly;
[0042] 3) Sterilize at 100°C for 10 minutes, then cool down to 30°C;
[0043] 4) Add 30L of Candida utilis culture medium (OD value 0.833) in the logarithmic growth phase to the sterilized extract solution at 30°C for expansion, the expansion temperature is 28°C, and the stirring speed is 60r / min, the ventilation rate is 12L / min, and the expansion time is 20 hours; the protein content in the kelp juice is 25.2g / L;
[0044] 5) After the expansion, heat the expansion solution to 50°C, add 200g of flavored protease, and enzymolyze it for 2 hours at 50°C, then use a hi...
Embodiment 3
[0048] A production method for improving the umami taste of kelp juice, comprising the steps of:
[0049] 1) Add 30kg of dried kelp into the extraction tank, inject 900L of demineralized water, soak for 50 minutes, raise the temperature to 75°C, and extract for 2 hours;
[0050] 2) Pump the collected 650L extract into the expansion tank, add 30Kg glucose and 3Kg glycine into the expansion tank, and stir evenly;
[0051]3) Sterilize at 100°C for 10 minutes, then cool down to 32°C;
[0052] 4) Add 20L of Candida utilis culture medium (OD value 0.856) in the logarithmic growth phase to the sterilized extract solution at 32°C for expansion, the expansion temperature is 28°C, and the stirring speed is 60r / min, the ventilation rate is 12L / min, and the expansion time is 20 hours; the protein content in the kelp juice is 23.6g / L;
[0053] 5) After the expansion, heat the expansion solution to 50°C, add 160g of flavor protease, and enzymolyze it for 2 hours at 50°C, then use a high-f...
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