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Method for rapidly activating and abundantly producing anabaena azotica ley dry powder

A technology of dried algae powder and anabaena, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problem of low germination rate of dry algae powder and inadequate rice field fertilizer function To achieve the effects of safe and efficient fertilizer application, easy promotion and implementation, and increased germination ratio

Inactive Publication Date: 2012-08-08
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prepared dry algae powder often has the disadvantage of low germination rate, so the fertilizer function of the paddy field cannot be fully exerted
[0005] So far, there are very few related studies on nitrogen-fixing anabaena dry algae powder at home and abroad. In terms of the activation of dry algae powder, only Huang Youxin et al. mentioned wheat The attachment of husk or rice husk can improve the germination effect, while the exploration in other aspects is rarely reported

Method used

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  • Method for rapidly activating and abundantly producing anabaena azotica ley dry powder
  • Method for rapidly activating and abundantly producing anabaena azotica ley dry powder
  • Method for rapidly activating and abundantly producing anabaena azotica ley dry powder

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Effect test

Embodiment 1

[0026] Weigh 0.01g of nitrogen-fixing Anabaena dried algae powder, dissolve it in a small beaker containing 10mL of basic culture solution, crush it with a 75% alcohol-sterilized algae maker for 3 times, each time for 10s, and then transfer the algae solution to In a small petri dish of 90×15 mm, rinse the beaker with the basic culture solution and pour it into the petri dish, and finally add the basic culture solution to the petri dish to 35mL. Add potassium nitrate to make the concentration 380-500mg / L, adjust the pH to 7 with sodium hydroxide and hydrochloric acid, and finally place it in a sterile room with a light intensity of 3000±200Lx and a temperature of 30±2°C for cultivation. Generally, after 2-3 days of culture, nitrogen-fixing Anabaena begins to be activated in large quantities. At this time, transfer the activated nitrogen-fixing Anabaena to an opaque open container, add an appropriate amount of basic culture medium, weigh an appropriate amount of sucrose, and add...

Embodiment 2

[0028] Weigh 0.01g of nitrogen-fixing Anabaena dried algae powder, dissolve it in a small beaker containing 10mL of basic culture solution, crush it with a 75% alcohol-sterilized algae maker for 3 times, each time for 10s, and then transfer the algae solution to In a small petri dish of 90×15 mm, rinse the beaker with the basic culture solution and pour it into the petri dish, and finally add the basic culture solution to the petri dish to 35mL. Add potassium nitrate to make the concentration 380-500mg / L, adjust the pH to 9 with sodium hydroxide and hydrochloric acid, and finally place it in a sterile room with a light intensity of 3000±200Lx and a temperature of 30±2°C for cultivation. Generally, after 2-3 days of culture, nitrogen-fixing Anabaena begins to be activated in large quantities. At this time, transfer the activated nitrogen-fixing Anabaena to an opaque open container, add an appropriate amount of basic culture medium, weigh an appropriate amount of sucrose, and add...

Embodiment 3

[0030] Weigh 0.01g of nitrogen-fixing Anabaena dried algae powder, dissolve it in a small beaker containing 10mL of basic culture solution, crush it with a 75% alcohol-sterilized algae maker for 3 times, each time for 10s, and then transfer the algae solution to In a small petri dish of 90×15 mm, rinse the beaker with the basic culture solution and pour it into the petri dish, and finally add the basic culture solution to the petri dish to 35mL. Add potassium nitrate to make the concentration 380-500mg / L, adjust the pH to 9 with sodium hydroxide and hydrochloric acid, and finally place it in a sterile room with a light intensity of 3000±200Lx and a temperature of 30±2°C for cultivation. Generally, after 2-3 days of culture, nitrogen-fixing Anabaena begins to be activated in large quantities. At this time, transfer the activated nitrogen-fixing Anabaena to an opaque open container, add an appropriate amount of basic culture medium, weigh an appropriate amount of glucose and add ...

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Abstract

The invention discloses a method for rapidly activating anabaena azotica ley dry powder. The method comprises the following steps of: a, preparing a basic culture solution from a certain amount of dipotassium phosphate, ferric citrate, molybdic acid and calcium carbonate; b, dissolving anabaena azotica ley dry powder into the basic culture solution to obtain an anabaena azotica ley solution; and c, putting the anabaena azotica ley solution into the basic culture solution, adding a certain amount of potassium nitrate, adjusting the pH to 7-9, and culturing for 2-3 days to realize rapid activation of anabaena azotica ley. A method for abundantly producing anabaena azotica ley activated with the method comprises the following steps of: transferring the activated anabaena azotica ley into an opaque open container filled with a basic culture solution of glucose or cane sugar; and culturing for 5-7 days to obtain abundantly-produced anabaena azotica ley. Due to the adoption of the method, the germination proportion of dry algae powder is greatly increased under an effective activating condition, the duration of germination is shortened, and the stress tolerance of the anabaena azotica ley is enhanced due to the use of the glucose or cane sugar.

Description

technical field [0001] The invention relates to a biological technology, in particular to a rapid activation of nitrogen-fixing Anabaena dry algae powder and a method for mass production thereof. Background technique [0002] Nitrogen-fixing Anabaena (Anabaena azotica) was provided by the Freshwater Algae Species Bank of the Institute of Hydrobiology, Chinese Academy of Sciences (No. FACHB-686), and has been published in "Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li; Molecular basis for differential metabolic responses to monosulfuron in three nitrogen-fixing cyanobacteria, Weed Science, 2009, 57: 178-188", "Jianying Shen, Jing Jiang, Peizhong Zheng; Effects of Light and Monosulfuron on growth and Photosynthetic Pigments of-Anabaquaena floss Breb, Water Resource and Protection, 2009, 1: 408-413", "Zheng Peizhong, Shen Jianying; Research on the Effect of Organic Solvent Acetone on the Growth of Nitrogen-fixing Cyanobacteria, Shanghai Agricultural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
Inventor 沈健英万旗东
Owner SHANGHAI JIAO TONG UNIV
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