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Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation

A feed additive, the technology of Aspergillus oryzae, applied to a strain of Aspergillus oryzae and its application field in the preparation of feed additives by microbial fermentation, can solve the problems of low protein and mineral content, low digestibility of livestock and poultry, high crude fiber content, etc., to achieve The effect of increasing crude protein content, increasing protein content, and strong enzyme system ability

Active Publication Date: 2014-03-19
安徽希普生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Take cellulose as an example. my country has abundant cellulose resources. The annual output of straw alone is about 570 million tons, but less than 10% of the amount is due to feed production. Due to the high content of crude fiber in straw feed and low protein and mineral content, Low digestibility of livestock and poultry, poor palatability, thus limiting its application

Method used

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  • Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation
  • Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation
  • Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: Screening and selection of strains

[0031] Isolation of Aspergillus oryzae with high activity of cellulase and protease

[0032] Isolation of Aspergillus oryzae from discarded plant samples such as straw. Prepare PDA medium (200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 mL of tap water, natural pH, sterilized at 121 °C for 20 min). Take a sample that may contain Aspergillus oryzae, grind it, add 1 gram to 99 mL of water, shake and mix well for 10 minutes, then take 100 μL and spread it on a PDA plate. After being placed at 25°C for 48 hours, the colonies were observed, and candidate Aspergillus oryzae strains were selected for isolation and purification of single spores. After further microscopic observation and identification, the strains were preserved for further experiments.

[0033] After multiple times of culture and mutation breeding, the screened strains were screened to obtain the strain 2013-DP1 with fermentation abilit...

Embodiment 2

[0040] Preparation of PDA medium: 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of tap water, natural pH, sterilized at 121°C for 20 minutes.

[0041] The cryopreserved Aspergillus oryzae strain CCTCC No: M2013276 was inoculated on PDA medium, placed in an incubator at 30°C, and activated for 72 hours.

[0042] Inoculate 3 to 5 single colonies of the activated strain into seed shake flasks (PDA medium, without agar), and culture them on a shaker at 200 rpm at 30°C for 24 hours to obtain seed liquid.

[0043] Take soybean meal, add water to 45% water content, sterilize at 121°C for 20 minutes to make fermentation medium, inoculate Aspergillus oryzae seed liquid at a volume ratio of 10%, and carry out aerobic fermentation for 64 hours (turn over once every 24 hours), After the fermentation is over, take a little fermentation product and dry it until the water content is about 10%, crush it and pass it through a 90-mesh sieve to test the content of each...

Embodiment 3

[0047] The activation and seed cultivation of Aspergillus oryzae strain CCTCC No: M2013276 are the same as in Example 2.

[0048] Take a mixture of soybean meal: bran: rice straw powder with a mass ratio of 1:1:1, add water to a water content of 40%, sterilize at 121°C for 20 minutes to make a fermentation medium, and inoculate the Aspergillus oryzae seed solution at a volume ratio of 10%. Carry out aerobic fermentation for 48 hours (turn the material once every 24 hours). After the fermentation is over, take a little fermentation product and dry it until the water content is about 10%.

[0049] Crude protein content of each raw material: soybean meal, 45.93%; bran, 14.57%; straw powder, 2.89%.

[0050] Among the fermentation products: water 11.00%, crude protein 25.63%, acid-soluble protein 4.89%.

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Abstract

The invention provides a new bacterial strain-Aspergillus oryzae 2013-DP1, and applications in preparation of feed additives through microbial fermentation. The strain is preserved in China Center for Type Culture Collection. The address is Wuhan University, Wuhan, China. The postcode is 430072. The preservation date is June 21th, 2013. The preservation number is CCTCC No: M2013276. Through fermentation and enzymatic hydrolysis conversion of low-value cellulose substances of straws and the like, the strain raises the protein content properly, raises nutritive values, can promote development of feed sources, raises the utilization rate of waste biological resources, and has obvious economic and ecological significances.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain obtained through screening and mutagenesis—Aspergillus oryzae (Aspergillus oryzae) 2013-DP1, and its application in microbial fermentation to prepare feed additives. (2) Background technology [0002] my country has a lot of people and little land. In recent years, with the rapid development of animal husbandry, the contradiction between humans and animals competing for food is quite prominent. my country has become a country with very tight feed resources, especially protein feed resources are very scarce. Since protein is one of the main nutritional components to meet the growth and development of livestock and poultry and improve nutrition and quality, and energy components constitute the main body of the feed, the protein content and quality of the feed determine the nutritional value of the feed. According to the best breeding technology, the amount of protein resources in my country's feed will ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/14A23K1/16C12R1/69
Inventor 朱廷恒陈新爱汪琨林聪崔志峰王渭霞
Owner 安徽希普生物科技有限公司
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