Preparation and application of aspergillus niger used for phosphate degrading, potassium degrading and cellulose degradation and microbial agent of aspergillus niger
A strain of Aspergillus niger, the technology of Aspergillus niger, which is applied in the field of preparation and application of Aspergillus niger and its bacterial agent, can solve the problems of large pores in the soil, incompatibility between soil and seeds, and affecting the direct return of straw to the field and large-scale promotion.
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Embodiment 1
[0042] 1. Isolation of CS-1 strain
[0043] Under sterile conditions, take 10g of wheat rhizosphere soil sample, dissolve in 90mL sterile water, 220r / min, vibrate for 30min, absorb 1mL and mix with 9mL sterile water to get 10 -1 diluent, then take 1mL 10 -1 The diluted solution was mixed with 9mL sterile water to obtain 10 -2 Dilutions, indistinctly analogy, were prepared 10 -3 , 10 -4 , 10 -5 Wait for a series of dilutions. Choose a dilution of 10 -3 、10 -4 、10 -5 Use a pipette to draw 100uL of the soil dilution solution in the center of the Martin's medium plate, spread the dilution solution evenly with a spreader, and culture it at a constant temperature of 30-32°C for 2-4 days, and pick the edge hyphae of different individual colonies Body, were streaked on the PDA plate culture, purification and preservation.
[0044] Several pure cultures obtained above were inoculated on the solid medium plate for inorganic phosphorus and potassium, and cultured at 30-32°C for ...
Embodiment 2
[0054] 1. Determination of the ability of CS-1 strain to decompose inorganic phosphorus
[0055] Inoculate the CS-1 strain on a PDA medium plate, place it in a constant temperature incubator at 30°C for 4-5 days, and wait until it is covered with dark brown spores, prepare a spore suspension with sterile water (the concentration of the spores of the CS-1 strain is 1.65×10 7 cfu / mL) was inoculated as a seed solution into a Erlenmeyer flask (250 mL) equipped with 50 mL of inorganic phosphorus-dissolving capacity determination fermentation medium, the inoculum size was 2% (volume ratio), and an equal amount of sterile water was inoculated as a control at the same time. Three repetitions were set for each treatment, 30°C, 200r / min shaking culture for 5 days, and the ability to dissolve inorganic phosphorus was measured. The measurement method was determined according to the molybdenum antimony anti-colorimetric method stipulated in the NY / T 1847-2010 guidelines, and the ability to...
Embodiment 3
[0068] Preparation of CS-1 microbial agent
[0069] (1) Strain activation: Streak inoculation of the CS-1 strain preserved on a low-temperature slant on a PDA medium plate, and incubate at 30°C±2°C for 3-4 days;
[0070] (2) Preparation of spore suspension: Inoculate the beaten fungus cake of Aspergillus niger CS-1 strain on a PDA medium plate, culture it statically at 30±2°C for 4-5 days, rinse the spores of CS-1 strain with sterile water, Collect and set aside; use a hemocytometer to count under a microscope, and dilute appropriately with sterile water to make the spore concentration reach 10 7 ~10 8 cfu / mL;
[0071] (3) Preparation of solid fermentation medium: The components of solid fermentation medium are: 300 g of bran, 75 g of soybean cake powder, and 375 mL of Mendels' nutrient solution; sterilize the solid fermentation medium at 121 ° C for 20 minutes, cool it, and set aside.
[0072] (4) Inoculation culture: Inoculate the CS-1 strain spore suspension into the sol...
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