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Preparation and application of a strain of Aspergillus niger that decomposes phosphorus, potassium and cellulose and its bacterial agent

A technology of Aspergillus niger and microbial inoculum, which is applied in the field of preparation and application of Aspergillus niger and its inoculum, which can solve the problems that the soil and seeds cannot be in close contact, which affects the direct return of straw to the field for large-scale promotion, and has many large pores in the soil.

Active Publication Date: 2020-11-24
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Straw returning directly to the field is an important way of straw returning to the field, but after the straw is directly returned to the field, the cellulose, hemicellulose and lignin rich in the straw are difficult to quickly decompose, resulting in large pores in the soil, and the soil and seeds cannot be in close contact , affect seed germination, rooting and emergence, etc., and seriously affect the large-scale promotion of straw returning directly to the field

Method used

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  • Preparation and application of a strain of Aspergillus niger that decomposes phosphorus, potassium and cellulose and its bacterial agent
  • Preparation and application of a strain of Aspergillus niger that decomposes phosphorus, potassium and cellulose and its bacterial agent
  • Preparation and application of a strain of Aspergillus niger that decomposes phosphorus, potassium and cellulose and its bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Isolation of CS-1 strain

[0043] Under sterile conditions, take 10g of wheat rhizosphere soil sample, dissolve in 90mL sterile water, 220r / min, vibrate for 30min, absorb 1mL and mix with 9mL sterile water to get 10 -1 diluent, then take 1mL 10 -1 The diluted solution was mixed with 9mL sterile water to obtain 10 -2 Dilutions, indistinctly analogy, were prepared 10 -3 , 10 -4 , 10 -5 Wait for a series of dilutions. Choose a dilution of 10 -3 、10 -4 、10 -5 Use a pipette to draw 100uL of the soil dilution solution in the center of the Martin's medium plate, spread the dilution solution evenly with a spreader, and culture it at a constant temperature of 30-32°C for 2-4 days, and pick the edge hyphae of different individual colonies Body, were streaked on the PDA plate culture, purification and preservation.

[0044] Several pure cultures obtained above were inoculated on the solid medium plate for inorganic phosphorus and potassium, and cultured at 30-32°C for ...

Embodiment 2

[0054] 1. Determination of the ability of CS-1 strain to decompose inorganic phosphorus

[0055] Inoculate the CS-1 strain on a PDA medium plate, place it in a constant temperature incubator at 30°C for 4-5 days, and wait until it is covered with dark brown spores, prepare a spore suspension with sterile water (the concentration of the spores of the CS-1 strain is 1.65×10 7 cfu / mL) was inoculated as a seed solution into a Erlenmeyer flask (250 mL) equipped with 50 mL of inorganic phosphorus-dissolving capacity determination fermentation medium, the inoculum size was 2% (volume ratio), and an equal amount of sterile water was inoculated as a control at the same time. Three repetitions were set for each treatment, 30°C, 200r / min shaking culture for 5 days, and the ability to dissolve inorganic phosphorus was measured. The measurement method was determined according to the molybdenum antimony anti-colorimetric method stipulated in the NY / T 1847-2010 guidelines, and the ability to...

Embodiment 3

[0068] Preparation of CS-1 microbial agent

[0069] (1) Strain activation: Streak inoculation of the CS-1 strain preserved on a low-temperature slant on a PDA medium plate, and incubate at 30°C±2°C for 3-4 days;

[0070] (2) Preparation of spore suspension: Inoculate the beaten fungus cake of Aspergillus niger CS-1 strain on a PDA medium plate, culture it statically at 30±2°C for 4-5 days, rinse the spores of CS-1 strain with sterile water, Collect and set aside; use a hemocytometer to count under a microscope, and dilute appropriately with sterile water to make the spore concentration reach 10 7 ~10 8 cfu / mL;

[0071] (3) Preparation of solid fermentation medium: The components of solid fermentation medium are: 300 g of bran, 75 g of soybean cake powder, and 375 mL of Mendels' nutrient solution; sterilize the solid fermentation medium at 121 ° C for 20 minutes, cool it, and set aside.

[0072] (4) Inoculation culture: Inoculate the CS-1 strain spore suspension into the sol...

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Abstract

The present invention relates to the preparation and application of a strain of Aspergillus niger that can decompose phosphorus, potassium, and cellulose, and its bacterial agent; the CS-1 strain has the ability to efficiently decompose phosphorus, potassium, and degrade cellulose; the CS-1 strain can Stable colonization in the rhizosphere of wheat, cucumber, apple and other crops and play its role in decomposing phosphorus, potassium and degrading cellulose; the bacterial agent prepared by using this strain can significantly promote the growth of crops; it can be applied to the production of microbial fertilizers, for It is of great significance to effectively reduce the amount of chemical fertilizer, improve soil fertility, increase crop yield and promote the direct return of straw to the field.

Description

technical field [0001] The invention relates to the preparation and application of a strain of Aspergillus niger capable of decomposing phosphorus, potassium and cellulose and its bacterial agent, and belongs to the technical field of agricultural microorganisms. Background technique [0002] Phosphorus is one of the necessary nutrient elements for plant growth and development. It is a component of nucleic acid, ATP, coenzyme and various enzymes in plants. At the same time, it participates in various physiological and biochemical processes in plants in various ways. and metabolism play an important role. General farmland soil is rich in phosphorus. However, it is estimated that two-thirds of the cultivated land in my country is deficient in phosphorus. The reason is that most of these phosphorus exist in the form of insoluble compounds that are not easily absorbed and utilized by plants. Therefore, it cannot meet the needs of general crops. growth needs. [0003] Potassium ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C05F17/20C12R1/685
CPCC05F11/08C12N1/14C05F17/20C12N1/145C12R2001/685Y02W30/40
Inventor 刘训理王晓辉王长栋隋君康李倩胡玉蓉纪超王昌乾宋鑫张嘉淼洒荣波
Owner SHANDONG AGRICULTURAL UNIVERSITY
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