Biochar loaded nano zero valent iron coupling phosphate solubilizing bacteria immobilized pellet, preparation method and application thereof

A technology for immobilizing pellets and phosphate solubilizing bacteria, applied in biochemical equipment and methods, microorganism-based methods, immobilizing on or in inorganic carriers, etc., can solve the problems of low processing efficiency and high cost, and achieve high-efficiency solutions. Phosphorus capacity, input reduction, effect of passivating heavy metals

Active Publication Date: 2019-12-20
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to develop a high-efficiency, ecologically-friendly method for preparing biochar-loaded nano-zero-valent iron-coupled phosphate-dissolving bacteria immobilized pellets for the problems of high cost and low processing efficiency in the prior art. and apply it to soil remediation

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  • Biochar loaded nano zero valent iron coupling phosphate solubilizing bacteria immobilized pellet, preparation method and application thereof
  • Biochar loaded nano zero valent iron coupling phosphate solubilizing bacteria immobilized pellet, preparation method and application thereof
  • Biochar loaded nano zero valent iron coupling phosphate solubilizing bacteria immobilized pellet, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0048] Example 1. Preparation of Biochar-carried Nano Zero-Valent Iron Coupled with Phospholytic Bacteria Immobilized Beads

[0049] 1) Add 4.5g of polyvinyl alcohol into 50mL of deionized water, heat it in a water bath at 40°C overnight, then raise the temperature to 80°C until it is completely dissolved, and sterilize it in a high-pressure steam sterilizer at 121°C and 110Kpa for 30min. Wait until the temperature drops to 40° C., add 0.5 g of sodium alginate sterilized by ultraviolet rays in advance, and stir evenly until completely dissolved.

[0050] 2) Carry out enrichment culture of non-decarboxylated Lerkelia (Leclercia adecarboxylata), and the liquid medium consists of 3.0 g of beef extract, 5.0 g of sodium chloride, 10 g of peptone, and 1000 mL of distilled water. A 150mL triangular conical flask filled with 50mL of beef extract peptone liquid medium was sterilized. Pick a ring of non-decarboxylated Lerekia stored in slant culture medium and inoculate it in beef extrac...

Embodiment 2

[0055] The performance measurement of the biochar-carrying nanometer zero-valent iron coupling phosphate-solubilizing bacteria immobilized pellet obtained in embodiment 2 and embodiment 1

[0056] 1) Mechanical strength: put 60 small balls into a 250mL Erlenmeyer flask, add 100mL deionized water, shake on a constant temperature oscillator for 12 hours, the ratio of intact small balls to the total number of original small balls indicates the strength coefficient of small balls .

[0057] 2) Swellability: Take 30 small balls, measure their diameter with a vernier caliper, then put them into a 250mL conical flask, add 100mL of deionized water, refrigerate at 4°C for 6h, and then measure their diameter in the same way: measured after expansion The ratio of the sum of the diameters of the pellets to the sum of the diameters of the original pellets indicates the expansion properties of the pellets.

[0058] 3) Mass transfer performance: The mass transfer performance of the immobili...

Embodiment 3

[0062] Determination of phosphorus solubilizing ability and heavy metal removal ability of biochar-loaded nano-zero-valent iron coupled with phosphate-dissolving bacteria immobilized pellets obtained in embodiment 3 and embodiment 1

[0063] (1) Determination of phosphorus solubilizing ability

[0064] 1) Pick a loop of non-decarboxylated Lecleria adecarboxylata cells stored in slant culture medium and inoculate it in beef extract peptone liquid medium, culture at 120 rpm, 37° C. for 24 hours to obtain seed liquid.

[0065] 2) Blot the surface moisture of the immobilized pellet soaked in physiological saline with sterile cotton, inoculate it in the same beef extract peptone liquid medium at 120 rpm, and incubate at 37° C. for 24 hours for activation.

[0066] 3) Inoculate the activated free bacteria and immobilized pellets into sterilized Ca 3 (PO 4 ) 2 In the liquid culture medium, and set up a control, place it in a 120rpm, 37°C incubator for 24h, centrifuge at 4500rpm fo...

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Abstract

The invention discloses a biochar loaded nano zero valent iron coupling phosphate solubilizing bacteria immobilized pellet, a preparation method and application thereof. The prepared immobilized pellet gives full play to the reduction and adsorption of lead by the biochar loaded nano zero valent iron, the resistance of phosphorus solubilizing bacteria to external heavy metal pressure is further improved, and the synergistic effect of the two greatly strengthens the passivation of lead. The immobilized pellet prepared by the method has the advantages of high mechanical properties, good mass transfer performance, outstanding phosphate solubilizing abilities, obvious lead passivation effects and the like. The immobilized pellet can be used for the stability of soil lead and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of remediation of heavy metal pollution, and in particular relates to a biochar-loaded nanometer zero-valent iron-coupled immobilized pellet of phosphate-dissolving bacteria and a preparation method and application thereof. Background technique [0002] Heavy metal pollution in farmland soil has great harm to the environmental ecosystem and human health. Heavy metals cannot be biodegraded and are easy to accumulate in organisms, and enter the human body along the food chain, thereby endangering human health and the balance of the ecosystem. On the other hand, due to the low utilization rate of phosphorus fertilizer applied to farmland soil in the current season, a large amount of insoluble phosphorus is accumulated in the soil, and the existence of soil phosphorus will affect the migration and transformation of heavy metals. Therefore, it is of great significance to effectively stabilize heavy metals in farmland soil,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/08B09C1/10C12R1/40C12R1/07C12R1/01
CPCB09C1/10C12N11/08C12N11/10C12N11/14
Inventor 李敏滕泽栋邵文张克瑶于福潞
Owner BEIJING FORESTRY UNIVERSITY
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