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Biochar-supported nano-zero-valent iron-coupled phosphate-solubilizing bacteria immobilized pellets and its preparation method and application

A technology of immobilizing small balls and phosphate-solubilizing bacteria, applied in biochemical equipment and methods, methods based on microorganisms, immobilized enzymes, etc., can solve the problems of low processing efficiency and high cost

Active Publication Date: 2021-09-14
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to develop a high-efficiency, ecologically-friendly method for preparing biochar-loaded nano-zero-valent iron-coupled phosphate-dissolving bacteria immobilized pellets for the problems of high cost and low processing efficiency in the prior art. and apply it to soil remediation

Method used

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  • Biochar-supported nano-zero-valent iron-coupled phosphate-solubilizing bacteria immobilized pellets and its preparation method and application
  • Biochar-supported nano-zero-valent iron-coupled phosphate-solubilizing bacteria immobilized pellets and its preparation method and application
  • Biochar-supported nano-zero-valent iron-coupled phosphate-solubilizing bacteria immobilized pellets and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation Example 1, the biological charcoal coupled nanoscale zero valent iron phosphorous bacteria immobilized beads embodiment

[0049] 1) 4.5g of polyvinyl alcohol was added to 50mL of deionized water, 40 deg.] C water bath overnight and then warmed to 80 deg.] C until complete dissolution, placed in autoclaved pan 121 ℃, 110Kpa sterilized 30min. Other temperature was lowered to 40 ℃, 0.5g of sodium alginate in advance of UV sterilized added thereto, stir until completely dissolved.

[0050] 2) decarboxylation of the non-bacterial lux (Leclercia adecarboxylata) enriched culture medium for the composition of the liquid beef extract 3.0g, NaCl 5.0g, peptone 10g, Distilled water 1000mL. 150mL Erlenmeyer flask filled with beef extract peptone broth 50mL, sterilization. Picked stored in a ring of non-slant medium was inoculated to a decarboxylation lux peptone beef extract broth, 120rpm, 37 ℃ solubilizing strain seed culture obtained was 24h.

[0051] The UV sterilized biolo...

Embodiment 2

[0055] 2, the performance obtained in Example 1 was measured biological charcoal coupled nanoscale zero valent iron phosphorous bacteria immobilized beads of Example

[0056] 1) Mechanical strength: the balls 60 into 250mL Erlenmeyer flask, 100mL of deionized water was added, shaken for 12h in the temperature oscillator, intact pellets as a percentage of the total number of original pellet represents pellet strength coefficient .

[0057] 2) expansion: Take 30 balls, whose diameter was measured with calipers, then placed in 250mL conical flask, 100mL of deionized water, 4 ℃ refrigeration 6h, and then measured in the same diameter: measured after expansion the sum of the ratio of the sum of the ball diameter of the ball diameter represents the original properties of expanded beads.

[0058] 3) the mass transfer properties: absorbance characterized immobilized beads of mass transfer performance. The pellets were washed with distilled water, blotted dry with filter paper, the surface...

Embodiment 3

[0062] Example 3, the resulting carrier 1 biochar coupled nanoscale zero valent iron phosphorous bacteria immobilized beads of solubilizing ability and the ability to remove heavy metals measured Example

[0063] Determination of (1) solubilizing ability

[0064] 1) picked in a storage ring slant medium non decarboxylation lux bacteria (Leclercia adecarboxylata) were inoculated in peptone beef extract broth, 120rpm, 37 ℃ 24h cultured seed liquid is obtained.

[0065] 2) saline soaked beads immobilized with dry sterile cotton its surface moisture, inoculated in the same peptone beef extract broth 120rpm, 37 ℃ activated for 24h.

[0066] 3) The activated bacteria and good free immobilized beads of 2% inoculum were inoculated into sterilized Ca 3 (Po 4 ) 2 Liquid medium, and control is provided, placed at 120 rpm, 37 ℃ incubator cultured 24h, centrifuged at 4500rpm 10min, the supernatant through 0.45um membrane solubilizing capacity measured by the molybdenum blue method.

[0067] Tr...

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Abstract

The invention discloses a biochar-loaded nanometer zero-valent iron-coupled phosphate-solubilizing bacteria immobilized pellet as well as a preparation method and application thereof. The immobilized pellets prepared by the present invention give full play to the reduction and adsorption of biochar-supported nano-zero-valent iron to lead, and also improve the resistance of phosphorus bacteria to external heavy metal pressure. The synergistic effect of the two greatly strengthens the resistance to lead. passivation. The immobilized pellets prepared by the method have the advantages of strong mechanical performance, good mass transfer performance, outstanding phosphorus dissolving ability, obvious lead passivation effect, etc., can be used for stabilizing soil lead, and have good application prospects.

Description

Technical field [0001] The present invention belongs to the field of remediation of heavy metals, in particular, to the immobilized beads and preparation method and application of a biological charcoal coupling nanoscale zero valent iron phosphorous bacteria. Background technique [0002] Heavy metal pollution of farmland soil ecosystem has on the environment and human health great harm, heavy metals can not be biodegradable, easy to accumulate in organisms, and enter the body with the food chain, and thus endanger human health and the ecosystem balance. On the other hand, since the application season into the low utilization of farmland soil phosphorus results in significant accumulation of insoluble phosphorus soil, and soil phosphorus presence status will affect migration and transformation of heavy metals. Therefore, an effective stabilizing heavy metals in soil is important, the development of efficient, economic and environmentally friendly heavy metal remediation technolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084B09C1/10C12R1/40C12R1/07C12R1/01
CPCB09C1/10C12N11/08C12N11/10C12N11/14
Inventor 李敏滕泽栋邵文张克瑶于福潞
Owner BEIJING FORESTRY UNIVERSITY
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