Aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as culture method and application thereof

A culture method, technology of Aspergillus oryzae, applied in the field of microbiology

Active Publication Date: 2014-07-23
SHANDONG UNIV
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Problems solved by technology

[0004] Known Aspergillus oryzae-derived β-fructosidase, only the report of inulin-type fructooligosaccharide synthesis, so looking for a new Aspergillus oryzae strain that can produce β-fructosidase of levan type fructooligosaccharide, to enzymatic method The synthesis of fructooligosaccharides is of great significance

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  • Aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as culture method and application thereof
  • Aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as culture method and application thereof

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[0007] A strain of Aspergillus oryzae (Aspergillus oryzae) FS4, deposited in the General Microbiology Center of the China Microbial Culture Collection and Management Committee on April 24, 2014, with the deposit number CGMCC No.9087, address: No. 1 Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences.

[0008] The Aspergillus oryzae FS4 strain grows rapidly on potato culture medium. The colony spreads in 2 to 3 days and produces a large amount of mycelium. The colony becomes white and partly yellow. It turns green after 7 days. The extension becomes brown to dark brown, and the hyphae within the base produce water-soluble brown pigment. The conidia head is radial, and the top capsule of the conidiophore is nearly spherical or flask-shaped, with double small stalks overlying. The conidia are pear-shaped or round when they are young, and are spherical or sub-spherical when mature, with rough and wrinkled surfaces. The 18S rDNA...

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Abstract

The invention relates to an aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as a culture method and application thereof. An aspergillus oryzae FS4 strain is collected in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2014, with the collection number of CGMCC No. 9087 and the address of Institute of Microbiology, Chinese Academy of Sciences, Building 3, NO.1 Beichen West Road, Chaoyang District, Beijing. The invention further relates to the culture method and application of the aspergillus oryzae strain. The aspergillus oryzae FS4 strain obtained by screening has the advantages of simple culture, stable genetic nature, high synthesis efficiency and stable enzyme activity, can be applied to the field of synthesis of levan type oligofructoses and the like by a modern bio-engineering technical enzyme method, and further has broad application prospects.

Description

Technical field [0001] The present invention relates to a strain of Aspergillus oryzae producing β-fructosidase and its cultivation method and application, and in particular to a strain of Aspergillus oryzae that can produce β-fructosidase (EC 3.2.1.26) and its cultivation method. It is used in the synthesis of levan-type oligofructose and belongs to the field of microbial technology. technical background [0002] Fructose oligosaccharide is a general term for fructose oligomers in which fructose groups are connected to sucrose (GF) molecules by β(2-1) bonds (inulin type) and β(2-6) bonds (levan type). Fructooligosaccharide is a new type of sweetener with health care functions such as regulating intestinal flora, proliferating bifidobacteria, promoting calcium absorption, regulating blood lipids, and anti-caries. At present, the production of oligofructose is mainly divided into plant extraction method and enzymatic method. [0003] The plant extraction method mainly uses chicory...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/24C12P19/14C12R1/69
Inventor 肖敏徐莉卢丽丽宋德勇王冬雪
Owner SHANDONG UNIV
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