Method for producing epigallocatechin and gallic acid through transformation of aspergillus niger whole cell

A technology of gallocatechin and whole cell transformation, which is applied in the fields of bioengineering and biochemistry, can solve the problems of high cost of enzymatic transformation and complicated process, and achieves the effects of simple transformation method, high transformation efficiency, and enhanced anticancer activity of the product.

Active Publication Date: 2015-06-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of enzyme conversion is high, and it is necessary to purchase enzyme preparations or prepare enzyme liquid by yourself, and the process is complicated

Method used

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  • Method for producing epigallocatechin and gallic acid through transformation of aspergillus niger whole cell
  • Method for producing epigallocatechin and gallic acid through transformation of aspergillus niger whole cell
  • Method for producing epigallocatechin and gallic acid through transformation of aspergillus niger whole cell

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Experimental program
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Effect test

Embodiment 1

[0031] Aspergillus niger (Aspergillus niger) RAF106 strain, the preservation date is August 27, 2014, the preservation number is CGMCC NO.9608, and the preservation unit is the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms; the preservation unit address is Beijing, China No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing (100101).

[0032] The formula of the seed medium of Aspergillus niger RAF106 strain is: 200g of potatoes, 20g of sucrose, 20g of agar, 1000mL of distilled water, natural pH, and sterilized at 121°C for 15min. Commercially available potato sucrose agar (PDA) medium can also be used.

[0033] The seed culture conditions of Aspergillus niger RAF106 strain were as follows: eggplant bottles were cultured statically, the culture temperature was 28°C, and the culture time was 4 days.

[0034] The method for obtaining the seed conidia of Aspergillus niger RAF106 strain is as follows: rinse the eggp...

Embodiment 2

[0039] Aspergillus niger (Aspergillus niger) RAF106 strain, the preservation date is August 27, 2014, the preservation number is CGMCC NO.9608, and the preservation unit is the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms; the preservation unit address is Beijing, China No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing (100101).

[0040] The formulation of the seed medium of Aspergillus niger RAF106 strain is: commercially available potato sucrose agar (PDA) medium.

[0041] The seed culture conditions of Aspergillus niger RAF106 strain were as follows: eggplant bottles were cultured statically, the culture temperature was 30°C, and the culture time was 3 days.

[0042] The method for obtaining the seed conidia of Aspergillus niger RAF106 strain is as follows: rinse the eggplant bottle covered with black Aspergillus niger conidia with sterile physiological saline, and gently scrape the surface of the medium...

Embodiment 3

[0047] Aspergillus niger (Aspergillus niger) RAF106 strain, the preservation date is August 27, 2014, the preservation number is CGMCC NO.9608, and the preservation unit is the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms; the preservation unit address is Beijing, China No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing (100101).

[0048] The seed medium formula of Aspergillus niger RAF106 strain was: 200g potato, 20g sucrose, 20g agar, 1000mL distilled water, natural pH, sterilized at 121°C for 15min. The seed culture conditions of Aspergillus niger RAF106 strain were: static culture in eggplant bottle, culture temperature 25℃, culture time 2d.

[0049] The method for obtaining the seed conidia of Aspergillus niger RAF106 strain is as follows: rinse the eggplant bottle covered with black Aspergillus niger conidia with sterile physiological saline, and gently scrape the surface of the medium with an inoculat...

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Abstract

The invention discloses a method for producing epigallocatechin and gallic acid through transformation of aspergillus niger whole cell. The whole cell biotransformation is performed by adopting the aspergillus niger; the whole cell biotransformation nutrient solution is a nutrient solution containing 1-20% of tea polyphenol or EGCG; the preservative number of the aspergillus niger RAF 106 strain is CGMCC NO.9608. The whole cell biotransformation nutrient solution comprises the following ingredients: 1-20% of tea polyphenol or EGCG, 0.03-0.8% of dipotassium phosphate, 0.1-3% of monopotassium phosphate, 0.03-0.7% of manganese sulfate, 0.3-2% of sodium chloride, 0.01-0.3% of magnesium sulfate and 0.02-2% of yeast or malt extract. By adopting the nutrient solution, the whole cell is transformed in one step, the transforming efficiency is high and the transformation can be finished within 20 hours, and the killing activity of the transformed product on the colon cancer cell is high.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and biochemistry, and in particular relates to a method for producing epigallocatechin and gallic acid by transforming whole cells of Aspergillus niger. Background technique [0002] As a globally popular plant beverage, tea beverage has been recognized for its health benefits in anti-cancer and cardiovascular diseases. At present, it is believed that catechin is the main functional active ingredient in tea, and its content accounts for 60-80% of the total tea polyphenols, including epicatechin (EC), epicatechin gallate (ECG), epigallocatechin Catechin gallate (EGCG), epigallocatechin (EGC), gallic acid (GA), gallocatechin gallate (GCG). The content of EGCG in tea is the highest, accounting for 9-13% of the dry weight of tea. Tea polyphenols have many important health effects, such as strong antioxidant activity and ability to scavenge free radicals, anticancer effect of inhibiting tumor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12P17/06C12R1/685
CPCC12P7/42C12P17/06
Inventor 方祥章爱媛刘文瑶
Owner SOUTH CHINA AGRI UNIV
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