36 results about "Aspergillus rugulosus" patented technology

The invention discloses a food-grade aspergillus niger strain FS-Z1 which is separated out from sauce grains and capable of preventing and treating zearalenone toxin. The collection number of the food-grade aspergillus niger strain FS-Z1 is CCTCC NO: M 2013703. The strain has excellent degradation effect on the zearalenone and the degradation rate reaches 89.56%. Besides, animal experiments in rats verify that the zearalenone in the corn steep liquor degraded by using an aspergillus niger fermentation liquor has no toxicity.

The invention provides a gene engineer strain of Aspergillus niger with high L-malic acid yield and a construction method thereof. The method utilizes gene knockout technique to disrupt the Aspergillus niger oxaloacetic acid hydrolase gene (oahA) so as to obtain the malic acid-producing strain S2. After 7 days of shake flask fermentation, 100 g / L glucose is converted into 120.4 + / - 2. 8 g / L of L-malic acid, the conversion of malic acid and malic acid to glucose is 1.62 mol / mol, which is 81% of the highest conversion in theory. A good strain for the industrialized production of malic acid is provided.

The invention discloses bacillus subtilis and an application of the same in resisting aspergillus. The bacillus subtilis has antagonistic action in aspergillus flavus. A method for screening the bacillus subtilis which has antagonistic action in the aspergillus flavus from soil comprises the following steps: a, bacteria isolating; b, aspergillus culturing and spore liquid preparing; c, aspergillus-resistant strain screening; and d, aspergillus-resistant strain rescreening. Through aflatoxin panel screening tests and toxin production aspergillus flavus antagonistic tests, aspergillus flavus antagonistic strains in the peanut cultivation fields and storage warehouses are screened. A method for reasonably and effectively screening the aspergillus flavus antagonistic strains is established, so that the appropriate required aflatoxin inhibition strains are obtained. The bacillus subtilis is applied to the easy aflatoxin contamination stages which are before and after harvesting crops of peanuts and the like and during storage periods and the like, the aflatoxin prevention and control levels of the crops of export peanuts and the like can be improved, export risks are lowered, and the export trade of the crops and products thereof are promoted.

The invention discloses aspergillus niger and a method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger. The aspergillus niger FOS-0620 is an aerobe, has black spores and milk-white hyphae, and is collected in China General Microbiological Culture Collection Centre (CGMCC for short) on September 28, 2012. The method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger comprises the following steps of: (1) preparing aspergillus niger whole-cells by virtue of the aspergillus niger FOS-0620 according to claim 1; and (2) catalytically producing fructo-oligosaccharide by virtue of the aspergillus niger whole-cells. The product obtained by the method disclosed by the invention is detected that the content (occupying total solids) of the fructo-oligosaccharide is not less than 50% via high-pressure liquid chromatography; and the method disclosed by the invention is simple in process, convenient to operate, high in enzymatic activity, high in conversion efficiency, and important in industrial value.

The invention discloses a strain of Aspergillus oryzae ZA116. The Aspergillus oryzae ZA116 is preserved in Guangdong Microbiological Culture Collection Center on November 15, 2018, the preservation number is GDMCC 60479, and the preservation address is 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The invention further provides application of the Aspergillus oryzae ZA116 in foodfermentation or koji making or soy sauce making. The strain ZA116 obtained through mutagenesis screening is high in autolysis rate in the fermentation stage, high in glutamic acid content in soy sauceand clearer in crude oil; the Aspergillus oryzae ZA116 with the high autolysis rate is adopted for improving the quality of soy sauce by fully releasing enzymes and nutrients in bacteria, the changeof an existing production process and replacement of production equipment are not involved, the production cost is low, and industrialized popularization is facilitated.

The invention discloses an aspergillus niger strain with a high yield of beta-D-fructofuranosidase and a liquid-state fermentation and enzyme production method of the aspergillus niger strain and belongs to the field of microorganisms or enzymes. The aspergillus niger strain is aspergillus niger QL-225 and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.13168; liquid deep-layer fermentation is carried out through a 50L fermentation tank; meanwhile, materials are supplemented and fermented and cultured for 120h to 140h; finally, the enzyme activity of fermentation liquid can reach 19000U / ml to 20000U / ml. According to the established method for producing the beta-D-fructofuranosidase through liquid fermentation, the most suitable operation pH (Potential of Hydrogen) of the produced beta-D-fructofuranosidase is 4.0 to 7.0, and the most suitable operation temperature range is 40 DEG C to 60 DEG C; the residual enzyme activity is 51.4 percent after heat is preserved at 70 DEG C for 8h; the aspergillus niger strain has good acid resistance and heat resistance and can be widely applied to the industrial fields of foods, medicines, wine brewing and the like.

The invention aims at providing a trichoderma reesei bacterial strain for producing saccharifying enzyme. The trichoderma reesei bacterial strain is trichoderma reesei HDGAU-1 (Trichoderma reesei HDGAU-1) with a preservation number of CCTCC (China Center for Type Culture Collection) NO: M2013584. The trichoderma reesei bacterial strain disclosed by the invention can efficiently express the saccharifying enzyme, and fermentation enzyme activity is as high as 4000 U / ml which is improved by 54% in comparison with that before mutation, and protein amount exceeds 2.1 g / L which is improved by about 50% in comparison with that before mutation. According to the invention, production cost of the saccharifying enzyme can be greatly lowered by utilizing the trichoderma reesei bacterial strain to produce the saccharifying enzyme, and wide application of the saccharifying enzyme is facilitated. Moreover, the saccharifying enzyme recombinant and expressed by the trichoderma reesei bacterial strain disclosed by the invention has an optimum acting temperature of 70 DEG C and an optimum acting pH of 5.5, has catalytic efficiency higher than that of the common saccharifying enzyme from aspergillus niger at present, is more high temperature resistant, can effectively shorten saccharifying time and can lower production cost.

The invention discloses a method for preparing a yeast culture through distillers' grains and an application of the yeast culture. The method comprises the following steps of S1, supplementing a carbon source to distillers' grains to obtain pretreated distillers' grains, inoculating mixed bacterium liquid to the pretreated distillers' grains, adding mixed enzyme liquid, preparing a fermentation base material, and performing fermentation culture for 96-120h; and S2, after fermentation is completed, performing inoculation with bacillus subtilis, continuing to perform fermentation culture for 24h, and performing baking, crushing and screening so as to obtain the yeast culture, wherein the mixed bacterium liquid comprises 3 strains of saccharomyces cerevisia, lactic acid bacteria and aspergillus niger, and the mixed enzyme liquid comprises liquefying amylase, glucoamylase, phytase and proteases. According to the method disclosed by the invention, the distillers' grains are used as substrates, the mixed bacterium liquid and the mixed enzyme liquid are added for heap fermentation, so that the yeast culture is obtained; and the distillers' grains are turned into wealth from waste, and besides, a feed rich in nutrient components is provided for animals, so that improvement of the immunity of the animals can be facilitated.

The invention discloses an Aspergillus niger strain and an application thereof. The strain is named Aspergillus niger ZJUQH2012147, which is collected in China General Microbiological Culture Collection Center (CGMCC) on May 23, 2012 with the collection number of CGMCC No. 6152. The invention further discloses a method for producing feruloyl esterase by using the strain, and the method comprises the following steps: (1) inoculating the Aspergillus niger ZJUQH201217 into a seed culture medium for propagation culture to obtain seed liquid; and (2) inoculating the seed liquid into a fermentationculture medium for fermentation culture. The method for producing feruloyl esterase by using the Aspergillus niger strain disclosed by the invention has high activity and is easy to realize industrial production of the feruloyl esterase. The process for producing the feruloyl esterase disclosed by the invention adopts soybean meal, wheat bran and other crude raw materials as fermentation raw materials, pretreatment of the crude raw materials is not required, and the production cost is low.

The invention discloses an aspergillus niger hydrolytic enzyme AnCu3. The amino acid sequence of the aspergillus niger hydrolytic enzyme AnCu3 is shown as SEQID No.1. The invention further discloses an application of the aspergillus niger hydrolytic enzyme AnCu3 to catalyzing and hydrolyzing of plasticizer dimethyl glycol phthalate (2-ethylhexyl) ester (DEHP) in a water phase system. The inventionprovides an enzyme which can catalyze and hydrolyze the dimethyl glycol phthalate (2-ethylhexyl) ester, and a coding gene for the first time, and a new way is provided for hydrolyzing of the dimethylglycol phthalate ( 2-ethylhexyl) ester.

The invention discloses Aspergillus niger, which is named as Aspergillus niger, has a strain number lm_2017, is preserved on July 18, 2017, and has a preservation number of CCTCC NO:M 2017428. The invention further discloses applications of the Aspergillus niger in fermentation of Moringa leaves. The fermentation steps comprise: inoculating the Aspergillus niger in an activation culture medium, activating, transferring the activated Aspergillus niger into a fermentation culture medium, and fermenting. According to the present invention, the Moringa leaves are fermented by using the Aspergillusniger, such that the flavor of the Moringa leaves is significantly improved, the Aspergillus niger probiotics in each g of the dry material are more than 8*10<8> cfu / g, and the product contains a variety of digestive enzymes so as to improve the nutritional value; and compared to the unfermented Moringa leaves, the fermented Moringa leaves have the following advantages that the protein content isincreased by 5-7%, the free amino acids are increased by 23.5-25.9%, and the bioavailability of the total amino acid is good, such that the feeding quality of the Aspergillus is improved.

The invention relates to aspergillus niger and an application thereof, and belongs to the technical field of bioengineering. On one hand, the invention provides the aspergillus niger ( aspergillus niger) WH-2, and the bacterial strain is preserved in the China General Microbiological Culture Collection Center ( CGMCC) at 03 December, 2018, wherein the preservation number is CGMCC No.16799, and thepreservation unit address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing. On the other hand, the invention provides an application of the aspergillus niger to preparation ofL(+)-tartaric acid or salts thereof, and a new method is provided for the L(+)-tartaric acid or salts thereof.

The invention provides an aspergillus niger strain with high rhamnosidase yield and an application thereof. The applicant firstly constructs the aspergillus niger engineering strain for recombinant expression of a rhamnosidase gene, and then further screens to obtain a mutant strain with high rhamnosidase yield through an ultraviolet mutagenesis method, and the preservation number of the mutant strain is CCTCC NO: M2019432. After the mutant strain is fermented in a 20L tank for 160h, the enzyme activity of rhamnosidase in the mutant strain fermentation supernatant reaches 1002 U / ml and is improved by 109.2% in comparison with that of the starting strain, and an unexpected technical effect is achieved. The aspergillus niger strain can be widely applied to production of rhamnosidase, so thatthe production cost of the rhamnosidase is reduced, and popularization and application of the aspergillus niger strain in the field of food processing are promoted.

The invention relates to Aspergillus niger pellets which are used for preventing and treating konjac southern blight and soft rot. Aspergillus niger xj is separated by fungal resource institute of Guizhou university, is collected in CCTCC (China Center for Type Culture Collection) (address: Wuhan University, Wuhan, China) at present and has the collection number CCTCCNO of M206021. The Aspergillus niger pellets contain Aspergillus niger fermented concentrate crude extract powder, microcrystalline cellulose (MCC), lactose and hydroxypropyl methylcellulose and can be used for preventing and treating konjac southern blight and soft rot.

The invention discloses a preparation method for an aspergillus niger spore suspension liquid. The method comprises preparing the aspergillus niger spore suspension liquid from aspergillus niger spore, and the pH value of the aspergillus niger spore suspension liquid is 1-2.5. The invention also discloses a storage method for aspergillus niger spore. The method comprises preparing the aspergillus niger spore suspension liquid from aspergillus niger spore, and the preparation method of the aspergillus niger spore suspension liquid is the method provided by the invention. By employing the methods disclosed by the invention, the bacterium-infection rate of aspergillus niger spore in the storage stage can be effectively reduced, so that the quality of a aspergillus niger seed liquid can be improved, and the fermentation level of citric acid can be improved.

The invention belongs to the technical field of microorganisms, and provides a quick yeast prepared from excellent aspergillus niger, rhizopus and saccharomycetes and a method for producing Shanxi mature vinegar by the cooperation of a quick yeast and gigantic yeast. The method comprises the following steps: carrying out multi-strain compounding on excellent indigenous strains of the aspergillus niger CGMCC15672, rhizopus microsporus CGMCC16969, saccharomyces cerevisiae CGMCC15729 and candida artemisiae CGMCC16913 in the fermentation process of Shanxi mature vinegar; obtaining the finished quick yeast by adopting a ventilated yeast bed for temperature control and drying at low temperature, wherein the enzyme activity, the acidity, the ester content, the storage period and the like of the finished quick yeast are obviously superior to those of the common quick yeast. According to the Shanxi mature vinegar produced by the cooperation of the quick yeast and the gigantic yeast, the utilization rate of the raw materials is greatly improved, the production cycle is shortened from 22 days to 15 days, the acidity of the obtained new vinegar is 4.85g / 100g, which is increased by 28.30% compared with the control, and the content of volatile aroma is enriched.

The invention discloses a high-hesperidinase-yield aspergillus niger KH005 and application thereof. The aspergillus niger KH005 with a preservation name being KH005 and a preservation number being CGMCC12101 is preserved in the China General Microbiological Culture Collection Center. The aspergillus niger is used for production of hesperidinase, degradation of hesperidin and production of hesperetin. The aspergillus niger KH005 is high in hesperidinase production efficiency, the activity is up to 2548U, valuable strain resources are provided for hesperidinase production, the produced hesperidinase can be used for degradation of hesperidin and production of hesperetin, and high conversion efficiency, mild and environment-friendly conditions and suitableness for industrial production are realized. In addition, the hesperidinase produced by the aspergillus niger KH005 has the advantages of high heat resistance, high enzyme activity and easiness in extraction and storage, thereby providing a new choice for production of high-purity commercial enzyme preparations and having high application and popularization values.

The invention discloses application of aspergillus niger in preparation of beta-fructofuranosidase. The aspergillus niger FOS-0620 has been deposited at the China General Microbiological Culture Collection Center with the accession number of CGMCC No. 6640. A preparation method comprises the following steps of filtering, washing and performing freeze drying, enzymolysis and cell wall breaking on the aspergillus niger FOS-0620 after the aspergillus niger FOS-0620 is fermented in an inducing fermentation medium to obtain beta-fructofuranosidase crude enzyme; and performing separation purification on the crude enzyme to obtain high-purity beta-fructofuranosidase. The prepared beta-fructofuranosidase is high in enzyme activity, and has a wide temperature application scope; and fructo-oligosaccharide which is catalytically prepared from the beta-fructofuranosidase by using sucrose as a substrate is simple in process and high in content, and reaches national standard.

The invention relates to a microbial metabolite capable of controlling nematodes and applications thereof, and belongs to the technical field of microbial pesticides. The metabolite of the invention is a compound ANMY16, and is obtained by fermenting and culturing an Aspergillus niger D16 bacterial strain and then isolating from a culture solution. The D16 bacterial strain is deposited in the China General Microbiological Culture Collection Center of the China Microbe preservation management committee, and assigned with the accession number CGMCC11340. The metabolite of the invention can killplant parasitic nematodes, and the control rate on the plant parasitic nematodes is greater than 75 percent.

The invention aims to provides an alkaline-lipase-producing Aspergillus niger mutant strain which is prepared by the following steps: transforming an alkaline lipase gene into an Aspergillus niger host strain to construct an Aspergillus niger engineering strain capable of efficiently expressing alkaline lipase; and knocking out the amylase gene of the host strain by gene knock-out means, and screening to obtain the mutant strain with obviously enhanced alkaline lipase yield. The collection number of the mutant Aspergillus niger strain is CCTCC NO:M2015761. The alkaline lipase activity of the mutant strain Aspergillus niger Su-12 reaches 15766 u / ml, and the protein expression level is 5.4 g / L, which are respectively enhanced by 56.3% and 18.9% as compared with the strain before mutation, thereby being beneficial to wide application of the enzyme.

The invention relates to a method for producing sodium gluconate by fermenting aspergillus niger. The method is characterized by comprising the steps that an aspergillus niger suspension is prepared;first nutrient salt is added into a seed tank with glucose as the raw material to prepare a seed culture solution, and the seed culture solution is inoculated into the aspergillus niger suspension; second nutrient salt is added into the seed tank with glucose as the raw material to prepare fermentation broth, the fermentation broth is inoculated into the seed solution for fermentation, in the fermentation process, mycelia are detected, the ventilation amount and the rotation speed are adjusted so that the length of aspergillus niger mycelia can be controlled in the range of 10-15 pm, and fermentation is completed when the glucose content in a fermentation tank is lower than 3 g / L; the fermentation broth is subjected to filtering, decoloring, crystallization, separation, drying and the liketo obtain a sodium gluconate finished product. Compared with an existing technology for producing sodium gluconate by fermenting aspergillus niger, the phenomena that the tank becomes yellow and smelly cannot be caused, the fermentation time can be shortened, and the yield and quality of sodium gluconate are improved.

The invention provides the application of an aspergillus versicolor HY12 strain in preparation of a fungal insecticide for preventing and controlling prodenia litura on tobacco. The insecticide is prepared from fermentation broth of the aspergillus versicolor HY12 and pesticide-acceptable auxiliary materials; the auxiliary materials are selected from one or more of a dispersing agent, a wetting agent, a disintegrating agent, an adhesive, a defoaming agent, an antifreezing agent, a thickening agent, filler and a solvent. The dosage forms of the insecticide are wetable powder, water-dispersiblegranules, water suspension or dispersible oil suspension. When the insecticide containing the aspergillus versicolor HY12 fermented broth, provided in the invention, is used for preventing and controlling the prodenia litura, the prodenia litura is enabled to turn into a pupal stage in advance, and the malformation rate and the mortality of the prodenia litura during the pupation are improved; theaspergillus versicolor HY12 strain has a certain inhibitory effect on the reproduction of the prodenia litura and lays the foundation for safe and effective prevention and control of the prodenia litura in the later period; in addition, the aspergillus versicolor HY12 strain provides an experimental basis for early application and promotion of prevention and control of insect disease insecticidesby utilizing an environment-friendly and healthy method.

The invention discloses a preparing method for Aspergillus oryzae protoplast, and relates to the technical field of methods for protoplast preparation in bioengineering. The method includes Aspergillus oryzae culture, hypha collection, enzymolysis and the like. By the adoption of the method, the yield of the protoplast can reach 1.5*10<7> / mL, the regeneration rate can reach 90%, and a foundation is laid for Aspergillus oryzae gene modification.

The invention relates to an Epicoccum purpurascens and Aspergillus spp. coculture method. The coculture method allows aspergillus to generate secondary metabolites which are lowly yielded or not generated in the single culture process. The method comprises the following steps: 1, respectively inoculating an activation medium with Epicoccum purpurascens and Aspergillus spp. to activate strains; 2, inoculating a solid medium in a same culture container with the activated Epicoccum purpurascens and Aspergillus spp., and carrying out stationary culture; or inoculating a liquid medium in the same culture container with the activated Epicoccum purpurascens and Aspergillus spp., and carrying out shaking culture; and 3, collecting the Aspergillus spp. and the media when Aspergillus spp. spores in the solid medium become yellow in order to obtain a product. The invention also relates to secondary metabolite produced through the coculture method.

The invention provides a method for cultivating aspergillus versicolor HY12 strains. The method comprises steps of preparation of aspergillus versicolor HY12 strain conidium suspension and fermentation cultivation. According to the method for cultivating aspergillus versicolor HY12 strains provided by the invention, by researching growth and propagation habits of the aspergillus versicolor HY12 strains, the aspergillus versicolor HY12 can achieve an extremely high growth and propagation rate within a short time by utilizing the cultivation method in the invention, and lots of conidiums can beproduced. The method has a high teratogenesis rate and fatality rate on prodenia litura, lays an experimental foundation for later control of the prodenia litura, and provides favorable reference forcontrol of other injurious insects in the field.