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Aspergillus sp. strain and application of aspergillus sp. strain to preparation of xylanase

A technology of xylanase and strain, applied to Aspergillus strain and its application field in preparing xylanase

Active Publication Date: 2013-11-13
广西鼎乐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Filamentous fungi have a strong ability to produce xylanase. Most of the reported fungi producing xylanase are concentrated in Aspergillus and Trichoderma. The reported fungi produced by Aspergillus The main products of xylanase hydrolysis of xylan are mostly xylooligosaccharides other than xylobiose, and there are no reports of xylanase produced by Aspergillus fumigatus with xylobiose as the main product and good temperature stability (Techapun C , Poosaran N.Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review.Process Biochemistry,2003,38:1327-1340; Sedlmeyer FB.Xylan as by- product of biorefineries: Characteristics and potential. Food Hydrocolloids, 2011, 25: 1891-1898)

Method used

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  • Aspergillus sp. strain and application of aspergillus sp. strain to preparation of xylanase
  • Aspergillus sp. strain and application of aspergillus sp. strain to preparation of xylanase
  • Aspergillus sp. strain and application of aspergillus sp. strain to preparation of xylanase

Examples

Experimental program
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Effect test

Embodiment 1

[0032]In Example 1, Example 2, and Example 3, xylanase activity was determined using 3,5-dinitrosalicylic acid (3,5-dinitrosalicylate, DNS) method (Miller GL. Use of dinitrosalicyclic acid reagent for determination of reducing sugar.Analytical Chemistry, 1959,31:426-428), the specific steps are as follows:

[0033] 1. Dissolve xylose in sterile deionized water to make xylose standard solutions of different concentrations (0.1mg / mL, 0.2mg / mL, 0.3mg / mL, 0.4mg / mL, 0.5mg / mL, 0.6mg / mL, 0.7mg / mL, 0.8mg / mL, 0.9mg / mL); add 1mL DNS to 500μL xylose standard solution, develop color in boiling water bath for 5min, cool to room temperature (25°C), measure light absorption at 540nm ; Obtain the standard curve of light absorbance and xylose concentration. The function formula of the standard curve is y=3.0549x+0.0008(R 2 =0.9990) (y is the light absorption value, x is the concentration of xylose).

[0034] 2. Add 250 μL of 1% birch xylan (Sigma company) solution to the 2mL EP tube, then ...

Embodiment 2

[0051] Embodiment 2, utilize Aspergillus fumigatus strain FC2-2 to prepare xylanase

[0052] 1. Obtaining of fermentation broth

[0053] 1. Preparation of fermentation medium

[0054] Prepare a liquid basic fermentation medium with a pH of 5.5 (recipe as above).

[0055] 2. Preparation of spore solution

[0056] (1) Prepare PDA solid medium and sterilize it at 121°C for 20 minutes.

[0057] (2) Wash the spores of the Aspergillus fumigatus strain FC2-2 obtained in Example 1 on the PDA solid medium plate for 5 days to make a spore suspension, wherein the spore concentration is 1×10 7 individual / mL.

[0058] 3. Obtaining of fermentation broth

[0059] (1) Put 50mL of liquid basic fermentation medium into a 250mL shake flask. Sterilize with damp heat at 121°C for 20 minutes.

[0060] (2) The spore suspension of Aspergillus fumigatus strain FC2-2 obtained in Example 1 was inoculated into the fermentation medium at an inoculum amount (volume percentage) of 1%, and cultured at...

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Abstract

The invention discloses an aspergillus sp. strain and application of the aspergillus sp. strain to the preparation of xylanase. The aspergillus sp. strain FC2-2 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6049. The invention also provides a method for producing the xylanase. The method comprises the following steps that the aspergillus sp. strain FC2-2 is subjected to liquid fermentation, fermentation products are collected, centrifugation is carried out for removing thalli, then, obtained fermentation supernatant can be used as a xylanase preparation, the most proper pH value of xylanase contained in the xylanase preparation is 5.5, the most proper temperature is 60 DEG C, the xylanase has better stability under the condition of 50 DEG C, and in addition, major products of hydrolysis bagasse xylanase are xylobiose. The xylanase preparation has application potential in the production of health care products of xylobiose.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an aspergillus strain and its application in preparing xylanase. Background technique [0002] In nature, lignocellulose is mainly composed of cellulose, hemicellulose and lignin. Hemicellulose is the second most abundant biomass resource on earth after cellulose, and its main component is xylan, which is composed of β-D-xylopyranose units through β-1,4- A linear polymer linked by glycosidic bonds. The complete degradation of xylan requires the synergy between various enzyme components in the xylan hydrolase system, including endo-1,4-β-D-xylanase (endo-1,4-β -D-xylanase, EC 3.2.1.8; also known as endoxylanase), β-xylosidase (β-xylosidase, EC 3.2.1.37), ferulic esterase (EC 3.1.1.73), etc., Among them, endoxylanase is the most critical enzyme for hydrolyzing xylan (Beg QK, Kapoor M. Microbial xylanases and their industrial applications: a review. Applied Microbiology and Biotechno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/68
Inventor 冯家勋杨齐罗雪梅徐强胜
Owner 广西鼎乐生物科技有限公司
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