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Preparation method for aspergillus niger spore suspension liquid and storage method for aspergillus niger spore

A technology of Aspergillus niger spores and storage method, which is applied in the field of preparation of Aspergillus niger spore suspension, can solve the problems of easy contamination and low contamination rate, and achieve the effects of improving fermentation level, reducing contamination rate and inhibiting growth

Active Publication Date: 2015-01-14
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of preparation method of the aspergillus niger spore suspension with low bacterial infection rate in the storage process in order to overcome the defect that the aspergillus niger gluten koji prepared by the existing method is easy to infect bacteria in the storage process, and a kind of How to store Aspergillus niger spores

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  • Preparation method for aspergillus niger spore suspension liquid and storage method for aspergillus niger spore

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preparation example Construction

[0022] According to the preparation method of Aspergillus niger spore suspension of the present invention, the method for adjusting pH value can be the method commonly used in this field, for example adjust with acidic reagent; The survival of Aspergillus niger spores is enough. Preferably, the acidic agent is selected from one or more of citric acid, hydrochloric acid and sulfuric acid. Since this spore suspension is then used to produce citric acid, more preferably the acidic agent is citric acid.

[0023] According to the preparation method of Aspergillus niger spore suspension of the present invention, the method that the Aspergillus niger spore in Aspergillus niger bran koji is made into spore suspension can adopt various methods well known to those skilled in the art, for example, can directly make Aspergillus niger bran Koji is mixed with sterile water and stirred to suspend the suspended spores of Aspergillus niger in sterile water to prepare a spore suspension.

[00...

Embodiment 1

[0043] This embodiment is used to illustrate the preparation method of the Aspergillus niger spore suspension and the storage method of the Aspergillus niger spores provided by the present invention.

[0044] (1) Put 600mL deionized water into a 1000mL triangular flask, adjust its pH value to 2.4 with citric acid, and sterilize the deionized water and inoculated steel cylinder after adjusting the pH value by high-temperature steam, and the sterilization pressure is 0.15Mpa , the sterilization temperature is 121°C, and the sterilization time is 60 minutes. Prepare sterile water at pH 2.0, and sterile inoculated cylinders. Reserve after natural cooling.

[0045] (2) Under sterile conditions, pour 300ml of the sterile water reserved in step (1) into the triangular bottle of bran (containing 20g of bran, the bran used to prepare bran is at 0.13Mpa, 121°C sterilized for 60 minutes), and stirred at 800 rpm for 45 minutes; then, at a frequency of 30,000, ultrasonically treated for ...

Embodiment 2

[0048] This embodiment is used to illustrate the preparation method of the Aspergillus niger spore suspension and the storage method of the Aspergillus niger spores provided by the present invention.

[0049] (1) Fill 600mL deionized water into a 1000mL Erlenmeyer flask, and adjust its pH to 2.1 with citric acid. The deionized water after adjusting the pH value and the inoculation cylinder were subjected to high-temperature steam sterilization, the sterilization pressure was 0.15Mpa, the sterilization temperature was 121°C, and the sterilization was 60min. Prepare sterile water with a pH of 1.8, and sterile inoculated cylinders. Reserve after natural cooling.

[0050] (2) Under sterile conditions, pour 300ml of the sterile water reserved in step (1) into the triangular bottle of bran (containing 20g of bran, the bran used to prepare bran is at 0.13Mpa, 121°C sterilized for 60 min), and stirred at 300 rpm for 60 min; then ultrasonically treated for 20 min at a frequency of 40...

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Abstract

The invention discloses a preparation method for an aspergillus niger spore suspension liquid. The method comprises preparing the aspergillus niger spore suspension liquid from aspergillus niger spore, and the pH value of the aspergillus niger spore suspension liquid is 1-2.5. The invention also discloses a storage method for aspergillus niger spore. The method comprises preparing the aspergillus niger spore suspension liquid from aspergillus niger spore, and the preparation method of the aspergillus niger spore suspension liquid is the method provided by the invention. By employing the methods disclosed by the invention, the bacterium-infection rate of aspergillus niger spore in the storage stage can be effectively reduced, so that the quality of a aspergillus niger seed liquid can be improved, and the fermentation level of citric acid can be improved.

Description

technical field [0001] The invention relates to a preparation method of aspergillus niger spore suspension and a storage method of aspergillus niger spores. Background technique [0002] Citric acid is safe and non-toxic, and is currently the world's largest fermented organic acid. It is widely used in chemical industry, medicine, environmental protection, food and other fields, such as sour agent, flavoring agent and preservative, preservative, buffer agent and chelating agent. It can be used as antioxidant, plasticizer and detergent in chemical industry, cosmetic industry and washing industry. It can be used as an anticoagulant in the pharmaceutical industry. [0003] The process of fermenting and preparing citric acid is usually: preparing Aspergillus niger bran koji, then inoculating Aspergillus niger bran koji into the seed medium of the seed tank for cultivation to obtain Aspergillus niger seed liquid, and then inoculating the Aspergillus niger seed liquid into the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/04C12R1/685
CPCC12N1/04C12N1/14
Inventor 熊结青卢宗梅钟华刘梦涵
Owner COFCO BIOTECHNOLOGY CO LTD
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