Application of aspergillus versicolor HY12 strain
A technology of Aspergillus versicolor and strains, applied in the application, insecticide, animal repellent and other directions, can solve the problems of loss of commercial value, production damage, harm, etc., and achieves easy manufacture and use, low cost, and good inhibitory effect. Effect
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Embodiment 1
[0023] Screening of Aspergillus versicolor HY12 strain
[0024] 47 soil samples were collected from the Spodoptera litura infested areas in southern Hunan tobacco fields, including 25 soil samples in Jianghua, Yongzhou, 16 soil samples in Hengyang, 4 soil samples in Jiangyong, and 2 soil samples in Ningyuan. Among them, the 5-point sampling method is used to collect soil samples, and the center distance between the two tobacco plants is selected as the soil sampling point, and the soil samples with a length of 10 cm, a width of 10 cm, and a depth of 5 cm are cut down with a soil shovel and put into a sterile self-sealing plastic bag. Take it back to the laboratory and store it in the refrigerator for separation. The time, location and related information of sample collection shall be recorded in detail, and different soil samples shall be marked separately.
[0025] The retrieved tobacco field soil samples were crushed under aseptic conditions and fully mixed, then fully mixe...
Embodiment 2
[0044] Evaluation of Laboratory Control Efficacy of Aspergillus versicolor HY12 Fermentation Liquid against Spodoptera litura
[0045] The Aspergillus versicolor HY12 strain obtained in Example 1 was identified, and the Aspergillus versicolor HY12 had a high similarity with multiple strains of Aspergillus, and the similarity coefficient with Aspergillus versicolor reached 99%. According to the rDNA ITS sequence, combined with its morphology The strain HY12 was identified as Aspergillus versicolor, wherein the rDNA ITS segment size of Aspergillus versicolor was about 500bp; SEQ ID NO.1.
[0046] Transfer Aspergillus versicolor HY12 into SDAY liquid medium, and incubate at 30°C for 30 hours to make the strain mother liquid; use a pipette to take 0.5mL strain mother liquid and add it to 20mL sterilized SDAY liquid medium, at 30°C, 200r / Min shaker culture 30h, made Aspergillus versicolor HY12 fermentation liquid, wherein the liquid medium is SDAY medium, wherein the SDAY medium i...
Embodiment 3
[0057] Evaluation of Field Control Efficacy of Aspergillus versicolor HY12 Fermented Liquid against Spodoptera litura
[0058] The field control effect evaluation test was carried out in Yujia'ao Tobacco Test Base, Ningxiang, Changsha City, Hunan Province. The test base has medium soil fertility, flat terrain, and convenient drainage and irrigation. See Table 1 for the details of the test agents and specific treatments. Clear water is used as the blank control, and it is arranged completely randomly. The area of each plot is 30m 2 , 4 repetitions. Spray pesticides during the full bloom stage of Spodoptera litura larvae. When spraying pesticides, the control plots are carried out first, and then the various pesticides are treated. Conventional spraying, the front and back of the leaves are evenly sprayed, and the base of the insect population is investigated 1 day before the pesticide. 1, 3, 5, 7d The number of live larvae in each treatment was investigated separately, and t...
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