Application of aspergillus versicolor HY12 strain

A technology of Aspergillus versicolor and strains, applied in the application, insecticide, animal repellent and other directions, can solve the problems of loss of commercial value, production damage, harm, etc., and achieves easy manufacture and use, low cost, and good inhibitory effect. Effect

Active Publication Date: 2019-04-26
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Spodoptera litura enters gluttony after the 4th instar and bites the leaves, leaving only the main veins. On the cabbage, the larvae can also drill into the leaf balls to cause harm, eat up the interior, and excrete feces, causing pollution and reducing it. loss of commodity value
Spodoptera litura pests occur all over the country, causing great damage to production

Method used

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  • Application of aspergillus versicolor HY12 strain
  • Application of aspergillus versicolor HY12 strain
  • Application of aspergillus versicolor HY12 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Screening of Aspergillus versicolor HY12 strain

[0024] 47 soil samples were collected from the Spodoptera litura infested areas in southern Hunan tobacco fields, including 25 soil samples in Jianghua, Yongzhou, 16 soil samples in Hengyang, 4 soil samples in Jiangyong, and 2 soil samples in Ningyuan. Among them, the 5-point sampling method is used to collect soil samples, and the center distance between the two tobacco plants is selected as the soil sampling point, and the soil samples with a length of 10 cm, a width of 10 cm, and a depth of 5 cm are cut down with a soil shovel and put into a sterile self-sealing plastic bag. Take it back to the laboratory and store it in the refrigerator for separation. The time, location and related information of sample collection shall be recorded in detail, and different soil samples shall be marked separately.

[0025] The retrieved tobacco field soil samples were crushed under aseptic conditions and fully mixed, then fully mixe...

Embodiment 2

[0044] Evaluation of Laboratory Control Efficacy of Aspergillus versicolor HY12 Fermentation Liquid against Spodoptera litura

[0045] The Aspergillus versicolor HY12 strain obtained in Example 1 was identified, and the Aspergillus versicolor HY12 had a high similarity with multiple strains of Aspergillus, and the similarity coefficient with Aspergillus versicolor reached 99%. According to the rDNA ITS sequence, combined with its morphology The strain HY12 was identified as Aspergillus versicolor, wherein the rDNA ITS segment size of Aspergillus versicolor was about 500bp; SEQ ID NO.1.

[0046] Transfer Aspergillus versicolor HY12 into SDAY liquid medium, and incubate at 30°C for 30 hours to make the strain mother liquid; use a pipette to take 0.5mL strain mother liquid and add it to 20mL sterilized SDAY liquid medium, at 30°C, 200r / Min shaker culture 30h, made Aspergillus versicolor HY12 fermentation liquid, wherein the liquid medium is SDAY medium, wherein the SDAY medium i...

Embodiment 3

[0057] Evaluation of Field Control Efficacy of Aspergillus versicolor HY12 Fermented Liquid against Spodoptera litura

[0058] The field control effect evaluation test was carried out in Yujia'ao Tobacco Test Base, Ningxiang, Changsha City, Hunan Province. The test base has medium soil fertility, flat terrain, and convenient drainage and irrigation. See Table 1 for the details of the test agents and specific treatments. Clear water is used as the blank control, and it is arranged completely randomly. The area of ​​each plot is 30m 2 , 4 repetitions. Spray pesticides during the full bloom stage of Spodoptera litura larvae. When spraying pesticides, the control plots are carried out first, and then the various pesticides are treated. Conventional spraying, the front and back of the leaves are evenly sprayed, and the base of the insect population is investigated 1 day before the pesticide. 1, 3, 5, 7d The number of live larvae in each treatment was investigated separately, and t...

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Abstract

The invention provides the application of an aspergillus versicolor HY12 strain in preparation of a fungal insecticide for preventing and controlling prodenia litura on tobacco. The insecticide is prepared from fermentation broth of the aspergillus versicolor HY12 and pesticide-acceptable auxiliary materials; the auxiliary materials are selected from one or more of a dispersing agent, a wetting agent, a disintegrating agent, an adhesive, a defoaming agent, an antifreezing agent, a thickening agent, filler and a solvent. The dosage forms of the insecticide are wetable powder, water-dispersiblegranules, water suspension or dispersible oil suspension. When the insecticide containing the aspergillus versicolor HY12 fermented broth, provided in the invention, is used for preventing and controlling the prodenia litura, the prodenia litura is enabled to turn into a pupal stage in advance, and the malformation rate and the mortality of the prodenia litura during the pupation are improved; theaspergillus versicolor HY12 strain has a certain inhibitory effect on the reproduction of the prodenia litura and lays the foundation for safe and effective prevention and control of the prodenia litura in the later period; in addition, the aspergillus versicolor HY12 strain provides an experimental basis for early application and promotion of prevention and control of insect disease insecticidesby utilizing an environment-friendly and healthy method.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to the application of a strain of Aspergillus versicolor HY12. Background technique [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in increasing agricultural production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (namely, resistance to pesticides, residence of pesticide residues, and Rampant Resurgence) has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests, especially in the past 10 years, the resistance of cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites to pyrethroids and organophosphorus chemical pesticides has incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04
CPCA01N63/30
Inventor 谭琳胡秋龙伍绍龙任佐华汤心砚樊吉君
Owner HUNAN AGRICULTURAL UNIV
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