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Microbial metabolite capable of controlling nematodes and applications thereof

A technology of microbial strains and nematodes, applied in the field of microbial metabolites

Inactive Publication Date: 2018-09-04
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compounds that kill nematodes with calcium oxalate complexes have not been reported in the literature. At the same time, since the D16 strain is an aquatic fungus (the inventor has applied for corresponding patent protection), not only the aquatic Aspergillus niger is rare, but it comes from the aquatic black fungus. The metabolites produced by Aspergillus have not been reported in other literatures

Method used

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  • Microbial metabolite capable of controlling nematodes and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Example 1. Obtaining metabolites:

[0021] 1. Cultivate the D16 strain on a PDA plate. When black spores grow on the plate, take it out for use;

[0022] 2. Collect the black spores on the plate under sterile conditions, insert the black spores into the PDA liquid culture containing glass beads, and culture at 28°C 150rpm / min;

[0023] 3. Cultivate for 2 days, and wait until the medium is covered with tiny mycelium balls, which are liquid seeds;

[0024] 4. Aseptically insert the grown liquid seeds into the pre-sterilized liquid PDA medium, the amount of insertion is calculated as 1% of the liquid volume of the new medium, and cultivate at 27°C and 180rpm / min for 4 days;

[0025] 5. Carry out the nematode bioassay of the fermented liquid with the nematode bioassay method in the content of the invention, after 120min, the nematode lethality rate reaches 80%, stop fermentation, it is D16 effective culture;

[0026] 6. Centrifuge the D16 liquid culture to remove bacteria...

example 2

[0029] Example 2, the nematicidal ability of metabolites:

[0030] Take the metabolite crystals and dissolve them in water, prepare solutions of 50 μg / ml, 100 μg / ml, 150 μg / ml, and 200 μg / ml respectively by weight ratio, and carry out nematicidal determination according to the method of "measurement of the effect of compounds on plant root-knot nematodes". After 4 hours of action, the lethality of root-knot nematodes for the solutions of four concentrations was 77%, 90%, 97%, and 100% respectively; after 12 hours of action, the lethality of root-knot nematodes exceeded 90%. Therefore, the application concentration of this metabolite acting on nematodes should be higher than 50 μg / ml.

example 3

[0031] Example 3. Application of metabolites to kill nematodes in the field:

[0032] Dissolve metabolite crystals in water at a weight percentage concentration of 100 μg / ml to prepare a 100 μg / ml solution. Taking tomato planting as an example, use 300ml of this solution to irrigate the roots of each tomato plant that has been transplanted for no more than 5 days, or spray 300ml of this solution per hole to treat the soil in the hole before transplanting. Investigate the occurrence of nematodes, and finally, the control rate of nematodes exceeds 75%.

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Abstract

The invention relates to a microbial metabolite capable of controlling nematodes and applications thereof, and belongs to the technical field of microbial pesticides. The metabolite of the invention is a compound ANMY16, and is obtained by fermenting and culturing an Aspergillus niger D16 bacterial strain and then isolating from a culture solution. The D16 bacterial strain is deposited in the China General Microbiological Culture Collection Center of the China Microbe preservation management committee, and assigned with the accession number CGMCC11340. The metabolite of the invention can killplant parasitic nematodes, and the control rate on the plant parasitic nematodes is greater than 75 percent.

Description

technical field [0001] The invention relates to a microbial metabolite capable of preventing and treating plant parasitic nematodes, belonging to the technical field of microbial pesticides. Background technique [0002] Plant parasitic nematodes are a common plant disease worldwide. There are more than 70 known types of root-knot nematodes alone, which damage more than 3,000 plants and cause huge losses every year. Plant parasitic nematodes in my country mainly harm economic crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, Panax notoginseng, American ginseng, etc., and the losses caused are astonishing. Among them, pine xylophilus is known as smoke-free forest fires, and vegetable root-knot nematodes are on the rise in major vegetable producing areas such as Shandong, Guangdong, Hainan, and Yunnan. American ginseng in Guizhou Province had to stop production due to severe occurrence of root-knot nematode. What's more serious is that root-knot nematode...

Claims

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Application Information

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IPC IPC(8): A01N37/04A01P5/00C12P7/44C12R1/685
CPCA01N37/04C12P7/44
Inventor 乔敏余泽芬
Owner YUNNAN UNIV
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