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Aspergillus niger strain and application thereof

A technology of Aspergillus niger and strains, applied in the directions of organic fertilizers, microorganisms, fungi, etc., can solve complex problems and achieve the effect of improving utilization rate, yield and quality

Inactive Publication Date: 2014-11-19
湖北明达科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus dissolving mechanism of fungi is very complex, mainly organic acid plays a role, and some strains produce other chelating substances during the growth process, which leads to the dissolution of phosphate rock powder

Method used

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  • Aspergillus niger strain and application thereof
  • Aspergillus niger strain and application thereof
  • Aspergillus niger strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening and isolation of the highly efficient phosphorus-solubilizing fungus Aspergillus niger (Aspergillus niger) pf1

[0028] The Aspergillus niger (Aspergillus niger) pf1 of the present invention is obtained by separating from soil by dilution plate method and plate streak method, and the separation method is as follows: screening by using Montina inorganic phosphorus solid medium. Soil sample collection, the sample collection location is Honghu City, Hubei Province, and the sampling time is February 13, 2013. The 5-point sampling method is used to collect the appropriate amount of soil within the range of 10-20cm in the surrounding and central areas of the plot. Mix well. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place in a shaker at 30°C at 150r / m for 10min, take 100μL of 10 -2 、10 -3 、10 -4 Spread the dilution on the inorganic phosphorus solid medium plate, and apply three paralle...

Embodiment 2

[0031] Example 2 Strain Identification of the Highly Efficient Phosphorus Solubilizing Fungus Aspergillus niger (Aspergillus niger) pf1

[0032] (1) Microbiological characteristics: On PDA medium, when cultured at 30°C, the colony of Aspergillus niger was white at first, spread rapidly, and then turned bright yellow until it was black velvet, with irregular edges, colorless on the back or central Slightly tawny. Mycelium has septa and branches, is multicellular mycelium, colored or colorless, has podocytes, apical capsule produces one or two layers of small stalks, and a string of conidia grows on the top of the small stalks, conidia The spore heads are brown-black and radial, the conidiophores are of different lengths, the top capsule is spherical, double-layered stalks, and the conidia are brown and spherical.

[0033] (2) Molecular biological characteristics

[0034] The rRNA gene sequence determination results of the strain (ITS-5.8S-ITS2 region) are as follows (SEQ No.1...

Embodiment 3

[0046] Example 3 Determination of Phosphorus Solubilizing Ability of Efficient Phosphorus Solubilizing Fungus Aspergillus niger (Aspergillus niger) pf1

[0047] The phosphorus-solubilizing ability of phosphate-solubilizing bacteria was determined by using Mengina inorganic phosphorus liquid medium. First, the Aspergillus niger (Aspergillus niger) pf1 preserved on the slant was inoculated into PDA liquid medium as seeds, and shaken at 30°C and 180r / min at a constant temperature. Shake culture in the bed for 12 hours, then inoculate the cultured bacterial solution into 250mL triangular flask containing 100mL Montina inorganic phosphorus liquid medium according to 1% inoculum amount (v / v) to inoculate 1% sterile PDA liquid Culture medium to Montina inorganic phosphorus liquid medium was used as a blank control, and cultured at 30°C and 180r / min in a constant temperature shaker for 7 days.

[0048] The molybdenum-antimony anti-colorimetric method was used to determine the availabl...

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Abstract

The invention discloses an Aspergillus niger strain and application thereof. The strain is Aspergillus niger pf1, and has a preservation number of CGMCC No.7600. The invention also discloses a microbial preparation containing the strain and a preparation method thereof. The original microbial powder of Aspergillus niger pf1 provided by the invention contains a large amount of phosphate solubilizing bacteria, which has certain positive effect on soil improvement, and the phosphorus solubilization function of the phosphate solubilizing bacteria has important significance in improving the utilization efficiency of phosphate fertilizer, and improving yield and quality of crops.

Description

technical field [0001] The invention belongs to the field of phosphorus-dissolving microbial fertilizers. Specifically, the present invention relates to a high-efficiency phosphorus-solubilizing fungus Aspergillus niger and its application, and a phosphorus-dissolving microbial bacterial agent prepared by using the Aspergillus niger. Background technique [0002] Phosphorus is one of the three major nutritional elements necessary for plant growth and development. Phosphorus is not only the basic constituent element of many organic compounds such as genetic material DNA and energy material ATP in plant cells, but also the photosynthesis of plants and the biochemical metabolism process in the body. Phosphorus plays an irreplaceable role in the whole life process of plants. Phosphorus is not only an important material guarantee for agricultural production, but also a non-renewable mineral resource. In my country, 74% of the arable land is deficient in phosphorus, and the seas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/685
Inventor 牛赡光杨光菊田龙军邓松华杨治家
Owner 湖北明达科技有限公司
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