4 results about "Feruloyl esterase" patented technology

Provided are the screening and identification of a Pseudomonas sp. strain for producing feruloyl esterase at high yield and a use of the Pseudomonas sp. strain as a bioaugmentation agent in fermented foods, relating to the technical field of brewing. By means of screening, a Pseudomonas sp. strain A-4H for producing feruloyl esterase at high yield is obtained. After cultivation for 36 h, the Pseudomonas sp. strain A-4H can produce ferulic acid at a concentration of 153.79±2.36 mg / L, and the activity of feruloyl esterase in a fermentation broth reaches 136.63±10.27 U / L. The addition of the Pseudomonas sp. A-4H during the brewing of Huangjiu can effectively increase the content of ferulic acid, the content of phenolic acid, and the content of organic acid in Huangjiu, providing an effective biological pathway for increasing the content of ferulic acid in fermented foods.

The application discloses a recombinant feruloyl esterase and a mutant and application thereof, and belongs to the technical field of enzyme engineering. Clostridium butyricum, The wild-type enzyme amino acid sequence is shown as SEQ ID NO:1, and the coding gene sequence is shown as SEQ ID NO:2; the target enzyme is obtained by constructing a recombinant expression vector containing the CbFAE gene, inducing expression in Escherichia coli BL21 (DE3) and purifying; substrate spectrum analysis shows that the enzyme not only has catalytic decomposition activity of model substrates such as methyl ferulate, but also has catalytic activity of decomposing mono (2-hydroxyethyl) terephthalate (MHET) into terephthalic acid (TPA) and ethylene glycol (EG); the application also constructs the CbFAE mutant L168R based on sequence and structure analysis, the amino acid sequence of the mutant enzyme is shown as SEQ ID NO:3, and the catalytic MHET decomposition activity of the mutant enzyme is increased by nearly 4 times compared with the wild type under the same reaction condition. The recombinant feruloyl esterase and the mutant provided by the application expand the types of MHET hydrolytic enzymes and provide a new way for the biodegradation of MHET.

The invention provides feruloyl esterase, a DNA molecule for coding the feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the feruloyl esterase in production of the feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. The feruloyl esterase BgFae2148 is subjected to genetic engineering modification to obtain a corresponding expression vector and an engineering bacterium, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

A method of treating a sunflower plant stem is disclosed. The method comprises the steps of:selecting a sunflower plant, removing the flower head and leaves from the stem and preparing the stem for enzymatic treatment by slitting the stem lengthways, removing the inner core from the stem;a) treating the cut stem with a pectinase containing a first buffer solution, for 24 hours with shaking at a pH of 3.8-8.5, and at a temperature of from 20-60° C.;b) removing the stem and washing with water,c) placing the stems into a second buffer solution containing xylanase, at a pH of from 7.0-8.0 and at a temperature of from 37-50° C. for 24 hours with shaking;d) removing the stems and washing with watere) placing the stems into a third buffer solution containing feruloyl esterase, at a pH of 7.2-8.0 containing 0.01-0.05% feruloyl esterase, at a temperature of from 37-50° C. for up to 24 hours,f) removing the stems and washing with water,g) pressing the stems to remove unwanted plant matter.