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Method for preparing ferulic acid from wheat bran by using two-enzyme method

A technology of wheat bran and ferulic acid, applied in the field of bioengineering, can solve problems such as the difficulty of separating ferulic acid, and achieve the effect of short cycle and low cost

Inactive Publication Date: 2014-03-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymatic hydrolyzate is a complex system, and it is still difficult to separate ferulic acid from it.

Method used

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  • Method for preparing ferulic acid from wheat bran by using two-enzyme method

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Experimental program
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Effect test

Embodiment 1

[0019] Example 1 Double Enzyme Extraction of Ferulic Acid in Wheat Bran

[0020] Accurately weigh 0.5g of wheat bran and place it in a 100mL Erlenmeyer flask. Add 8mL reAorFaeA enzyme solution (about 16.88U) and 0.5mL reAorXynllA enzyme solution (about 72.5515U), dilute to 30mL with 0.1mmol / L, pH 5.0 phosphate buffer, enzymolyze at 40°C for 10h, boil for 5min Inactivate the enzyme activity, centrifuge and collect the supernatant as the ferulic acid crude extract, the crude extract is concentrated to 1mL by rotary evaporation, and vacuum dried to obtain the ferulic acid crude extract powder, weighing 1.75g, detected by HPLC, ferulic acid The content is 0.13%.

Embodiment 2

[0021] Embodiment 2 macroporous resin purifies ferulic acid crude extract

[0022] Concentrate the crude extract of ferulic acid until the mass concentration of ferulic acid is 0.1 mg / mL, adjust the pH of the concentrated solution to 4.0, select HPD-300 macroporous resin, and pack 20 mL of resin into a column by wet method. After dynamic adsorption of 100mL of the crude extract on the column at 1mL / min, let stand for 2h, wash the column with water until the effluent is colorless, elute with 100mL of 50% ethanol at a flow rate of 1mL / min, and concentrate the eluent to 1mL by rotary evaporation. Vacuum dried into powder, weighing 431mg, detected by HPLC, the content of ferulic acid was 22.5%, and the recovery rate was 97%.

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Abstract

The invention provides a method for preparing ferulic acid from wheat bran by using a two-enzyme method. Wheat bran is subjected to enzymolysis by utilizing the synergistic effect of reAorFaeA and reAoXynllA from a laboratory, so as to release ferulic acid and obtain a crude ferulic acid extract. The crude ferulic acid extract is purified by using an HPD-300 weal-polarity macroporous resin, and the fraction with the ferulic acid obtained from purification is dried in vacuum so as to obtain a ferulic acid product. By adopting the method, raw materials for extracting and preparing the ferulic acid are cheap and easily available, in the product purity is high, the preparation process is simple, and the method is simple, convenient and feasible in operation, environment-friendly and applicable to industrial expansion production.

Description

technical field [0001] The invention relates to a method for preparing ferulic acid from wheat bran by using biological enzymes, belonging to the technical field of bioengineering. Background technique [0002] my country is a large agricultural production country. The annual output of cell wall substances of agricultural and sideline products such as wheat bran, corncobs, and rice bran is about 30 million tons, and most of them are discarded or used as animal feed. The comprehensive utilization rate is low and the economic value is not high. The chemical name of ferulic acid is 4-hydroxy-3-methoxycinnamic acid, which is one of the derivatives of cinnamic acid, and its content is relatively high in grains such as wheat bran, rice bran, and bagasse. It is a Natural antioxidants have physiological effects such as inhibiting platelet aggregation, promoting platelet dissolution, anti-thrombosis, anti-oxidation, and anti-tumor, and are valued in the pharmaceutical and food indust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C07C59/64C07C51/47
Inventor 邬敏辰曾妍王春娟殷欣李剑芳
Owner JIANGNAN UNIV
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