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Compound feruloyl esterase additive for feed and using method thereof

A ferulic acid esterase and additive technology, applied in the field of compound enzyme preparations for feed, can solve the problems of not finding or ignoring the side chain hydrolase effect, not determining the addition ratio and use method, etc., achieving high practical application value and improving Biological transformation efficiency, promotion of increased effect

Inactive Publication Date: 2008-07-30
CHINA AGRI UNIV
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Problems solved by technology

The main reason is that, firstly, the rumen fermentation characteristics of ruminants are not considered to rationally select the appropriate enzyme types and enzyme activity compatibility parameters; secondly, the enzymes including ferulic acid esterase, which eliminate lignin in the feed cell wall, have not been found or ignored The efficacy of the side chain hydrolase; moreover, the appropriate addition ratio and method of use have not been determined

Method used

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Embodiment 1

[0019] Embodiment 1 Preparation of compound ferulic acid esterase additive

[0020] The first step: select four kinds of enzyme preparation raw materials as listed in Table 1, and after the various enzyme activities of these four kinds of enzyme preparations (numbers are respectively E12, E7, E16, E8) raw materials are measured, the results are shown in Table 1. E12 is cellulase, the measured exo-cellulase activity is >5000U / g, E7 is xylanase, the measured xylanase activity is >10000U / g, E16 is β-glucanase, the measured β- Glucanase activity > 1000U / g, E8 is ferulic esterase, measured ferulic esterase activity > 4U / g. The above raw materials meet the enzyme activity requirements of the enzyme preparation required by the present invention.

[0021] Table 1. The measured results of different enzyme activities of four commercial enzyme preparation raw materials (U / g)

[0022] serial number

Commercial enzyme name

CMCase

SCELL

BX

BG

FAE

E1...

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Abstract

The invention provides a feeding complex ferulic acid esterase additive, which comprises four enzymes of ferulic acid esterase, cellulase, xylanase and glucanase; among which, the enzyme activity of endo cellulose is more than 16U / g, the enzyme activity of xylanase is more than 1024U / g, the enzyme activity of beta-glucanase is more than 2.0U / g, and the enzyme activity of ferulic acid esterase is more than 2.0U / g. The feeding complex ferulic acid esterase additive of the invention has the advantages of remarkably improving the release amount of reducing sugar, the cell wall degrading rate and the vitro rumen fermentation volatile fatty acids yield in the hydrolysis process of forage plants, crop straws and bran feed, enhancing the feed biological transfer rate in an all-round way. The invention has a high practical application value in the feed industry and the ruminant breeding industry.

Description

technical field [0001] The invention relates to a feed compound enzyme preparation, in particular to a feed compound ferulic acid esterase additive for promoting feed cell wall degradation and a use method thereof. Background technique [0002] Ferulic acid esterase (E.C.3.1.1.73, also known as cinnamic acid esterase, cinnamic acid hydrolase) is a class of enzymes that hydrolyze the ester bond between hydroxycinnamic acid and sugar in the plant cell wall, and the main reaction it catalyzes is : polysaccharide-ferulic acid ester+H 2 O = ferulic acid + polysaccharides (including some derivatives of ferulic acid). Ferulic acid esterase has a wide application space in papermaking, food, pharmaceutical and feed industries. Forage grass, crop stalks, and bran feed dry matter contain up to 30-80% cell wall components. However, due to the presence of lignin in cell walls, ruminants have a very limited ability to digest these feeds. Ferulic acid esterase can open the dense network...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K20/189
Inventor 杨红建谢春元
Owner CHINA AGRI UNIV
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