45 results about "Ferulic acid esterase" patented technology

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

The invention discloses a novel ferulic acid esterase whose amino acid sequence is shown in SEQ ID NO.3; the invention further discloses a DNA of the novel ferulic acid esterase, and a nucleotide sequence of the DNA is shown in SEQ ID NO.1. The invention also discloses an expression vector containing the DNA of the above novel ferulic acid esterase, a recombined ferulic acid esterase, a preparation method of the recombined ferulic acid esterase, and applications of the recombined ferulic acid esterase in degrading plant cell walls. The novel ferulic acid esterase and the recombined ferulic acid esterase of the invention have high efficient soluable expression in an Escherichia coli expression system. The recombined ferulic acid esterase can release 16.3 plus or minus 0.9mu. g of ferulic acid from 20mg of discarded starch wheat bran, and the released ferulic acid is 16% of the total amount of the ferulic acid.

The invention relates to the technical field of probiotics and particularly relates to lactobacillus reuteri and an application thereof. The invention provides Lactobacillus reuteri SLZX19-12 which ispreserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Lactobacillus reuteri SLZX19-12 is CGMCC No. 20121. Lactobacillus reuteri SLZX19-12 can be planted in an animal body, and is high in acid resistance and cholate resistance; the good inhibition effect is achieved on escherichia coli and salmonella; feruloyl esterase can be generated, and release and digestion of fibers in the feed in digestive tracts are promoted; the lactobacillus reuteri SLZX19-12 is prepared into a microbial inoculum and then fed to animals, intestinal floracan be improved, intestinal immunity can be improved, intestinal health can be improved, and the effect is safe and reliable.

The invention belongs to the application field of biotechnology and particularly relates to a method for improving the extraction rate of ferulic acid in plant straws by steam explosion pretreatment. The method includes the following steps: the plant straws containing 10 to 25 percent of water are treated by steam explosion under the condition that the steam pressure is 0.9 to 1.9 MPa, and then the plant straws are air dried; with the synergistic effect of ferulic acid esterase and the degrading enzyme of lignocellulose material, the air dried plant straws processed by steam explosion is carried out zymohydrolysis to obtain the enzymolysis liquid of the ferulic acid. The steam explosion technology is clear and high efficient without pollution. Combined with enzymatic extraction, the method improves nine to twelve times of the release rate of the ferulic acid in the plant straws.

The invention aims to provide a method for reforming heat stability of ferulic acid esterase A, constructing a mutant ferulic acid esterase A engineering bacterium and realizing high-efficiency heterologous expression of mutant ferulic acid esterase A. According to the space structure design mutant A126C and N152C derived from A.usamii E001 ferulic acid esterase A (AuFaeA), mutant ferulic acid esterase A: AuFaeA A126C-N152C is obtained, the mature peptide gene sequence thereof is SEQ ID NO:1, and C126 and C152 can form one disulfide bond. Experimental results indicate that the heat stability of the enzyme is obviously improved after mutation, thus providing a new technological path for reforming of other enzymes. As a heat-resistant enzyme preparation, the ferulic acid esterase has great industrial production potential and economic value.

Disclosed is a process that uses selective enzymes from microbial or plant sources to facilitate the extraction of hemicellulose. Selective removal of acetate using acetyl xylan esterase (E.C.3.1.1.6) under conditions that will not hydrolyse the feruoylate esters. This treatment renders the arabinoxylan ferulate soluble under mild aqueous conditions without the use of harsh alkalis. Arabinoxylan ferulate is extracted intact and subsequently the level of ferulic acid can be controlled to give the degree of crosslinking required by treatment of the soluble arabinoxylan ferulate with ferulic acid esterase.

The invention relates to a wheat based diet specific xylan degradation compound enzyme preparation. The active component of the wheat based diet specific xylan degradation compound enzyme preparation comprises xylanase and arabinfuranosidease and / or ferulic acid esterase. The invention aims at preparing the wheat based diet specific araboxylan degradation compound enzyme preparation according to the structural features of the wheat araboxylan, and the utilization effect of poultry to wheat is targetedly improved. By means of the specific xylan degradation compound enzyme preparation, the utilization effect of the wheat is improved, proliferation of beneficial bacteria such as intestinal lactic acid bacteria and the like is further facilitated, intestinal health is achieved, and the production performance of the poultry is greatly improved.

Electroporation methods for transferring a nucleic acid of interest, including ferulic acid esterase nucleic acid, into bacterial host cells, such as Lactobacillus spp. are disclosed. Compositions of transformed bacterial host cells, such as transformed Lactobacillus buchneri, and use assays are provided.

The invention provides a Kluyveromyces marxianus recombinant strain capable of being used for preparing feruloyl esterase AnfaeA. The recombinant strain is constructed by cloning an aspergillus nigerferuloyl esterase AnfaeA gene with an optimized sequence to an expression vector and converting a Kluyveromyces marxianus host strain, and the yield of the feruloyl esterase obtained by high-density fermentation of the recombinant strain reaches 120000 U / ml. The invention also provides a method for preparing ferulic acid by hydrolyzing corncob powder with the feruloyl esterase. The feruloyl esterase AnfaeA prepared from the recombinant strain and xylanase have a synergistic effect, so that the corncob powder can be efficiently hydrolyzed to obtain the ferulic acid. The feruloyl esterase provided by the invention can be used for preparing phenolic substances such as ferulic acid, p-coumaric acid and the like through biodegradation of agricultural byproducts such as corncobs, rice bran, cornbran and the like, so that the feruloyl esterase has a wide application value in the fields of foods, papermaking, feeds, medicines and the like. Meanwhile, the preparation method is simple in process, high in yield and very suitable for large-scale production.

The invention relates to application of ferulic acid and oligosaccharide preparation by producing enzymes from Salicornia bigelovii straws fermented by microorganism. The microorganism in the invention refers to Aureobasidium pullulans; the Salicornia bigelovii straws are dried and crushed to a certain particle diameter, and then a mixed enzyme preparation of xylanase and ferulic acid esterase is generated after the metabolism of the Aureobasidium pullulans; the cell walls of the Salicornia bigelovii straws are degraded under the synergistic effect of the mixed enzyme so as to obtain physiologically active substances, such as the ferulic acid and oligosaccharide. The invention has the effects of oxidation resistance, free radical elimination, thrombus resistance, blood fat reduction, coronary heart disease prevention and cure, bacteria resistance, inflammation elimination, mutation resistance and cancer prevention, can be widely applied in industries of medication, food and cosmetics, and can prominently increase the economic value of the straws, i.e., by-products of Salicornia bigelovii.

The invention discloses an application of arabinosidase in preparing wheat bran dietary fiber as well as wheat bran flour and wheat bran steamed buns, which are prepared from the arabinosidase. The application method comprises the following steps of: (1) preparing an enzyme solution of arabinosidase; (2) performing ultra-fine crushing on wheat bran; (3) adding the enzyme solution to the crushed wheat bran, and performing hydrolysis; and (4) after hydrolysis, performing heating, performing boiling-over and performing cooling to room temperature so as to obtain wheat bran dietary fiber. The method is simple and convenient; by using the arabinosidase or the combined action of the arabinosidase and xylanase and ferulic acid esterase, the wheat bran is directly hydrolyzed to obtain the wheat bran dietary fiber; the obtained wheat bran dietary fiber is higher in water holding capacity, oil holding capacity and oxidation resistance; besides, the gluten network structure in the prepared wheatbran flour fully absorbs water and the gluten structure is more stable; and the prepared wheat bran steamed buns not only contain rich wheat dietary fiber and vitamins, but also are good in leaveningproperties, loose and soft in structure, good in palatability, nutritional, healthy, and unique in wheat taste.

The invention provides a strain of Aspergillus oryzae for producing feruloyl esterase and application thereof. The Aspergillus oryzae has the category name of Aspergillus oryzae M3, is collected by China center for type culture collection, called CCTCC for short, at December 8, 2013 and has the collection number of CCTCC NO: M2013640. According to the strain provided by the invention, a method that feruloyl esterase is produced through fermenting by taking wheat bran as a fermentation substrate and bound-type phenolic and ferulic acids are obtained through rupturing ester bonds between the polysaccharide and lignin of plant cell walls and ferulic acid by the feruloyl esterase is adopted, and the condition that microorganisms are short in growth cycle, are not restricted to climate and are easily subjected to large-scale production is utilized, so that a novel biological method for extracting bound-type phenolic acid substances, such as ferulic acid is provided.

The invention discloses ferulic acid esterase EpFAE1 and a coding gene and an application thereof.The amino acid sequence of the ferulic acid esterase EpFAE1 is shown as SEQ ID NO.1, and the DNA sequence of the coding gene of the ferulic acid esterase EpFAE1 is shown as SEQ ID NO.2.According to the ferulic acid esterase EpFAE1, a coding gene of the ferulic acid esterase EpFAE1 is cloned from finepenicillium 4-14, and a recombinant vector of the ferulic acid esterase EpFAE1 is expressed by Pichiapastoris to obtain pure enzyme.Experiments prove that the ferulic acid esterase EpFAE1 has the advantages of high specific activity, high enzymolysis efficiency, high stability; certain metal ion resistance, salt resistance and the like, and have an important application prospect in the fields of medicine, health care, food, feed, biological energy and the like.

The invention discloses a lactobacillus plantarum strain separated from silage and used for producing ferulic acid esterase. The lactobacillus plantarum strain has the preservation number of CGMCC12955, has the ability to produce the ferulic acid esterase, and can maintain good stability at the pH of 5-7 and the temperature of 25-50 DEG C, optimally, at the pH of 6.4 and the temperature of 37 DEGC. The lactobacillus plantarum strain can be applied to preparation of silage additives and preparation of health care foods and medicines.

A method of improving milk yield in a ruminant animal a ruminant ration with an aqueous is presoaked with a solution of a composition of enzymes comprising a xylanase and a ferulic acid esterase.

The invention discloses lactobacillus brevis for producing feruloyl esterase, which is separated from silage. The lactobacillus brevis has the preservation number of CGMCC12957 and has a capability ofproducing the feruloyl esterase; the optimal pH (Potential of Hydrogen) is 6.4 and the optimal temperature is 37 DEG C; and the lactobacillus brevis can keep relatively good stability under the conditions that the pH is 5 to 7 and the temperature is 25 to 50 DEG C. The strain provided by the invention can be applied to preparation of a silage additive and preparation of ferulic acid.

The invention provides a separation method used for producing ferulate enzyme microorganism, which cultures the sample with ferulate as culture medium of carbon source, wherein the microorganism producing ferulate enzyme can utilize ferulate as carbon source so as to form bacterial colony, nevertheless the microorganism which can't utilize ferulate can't grow so as to achieve the aim of separating the microorganism producing ferulate enzyme. The invention completes separation of microorganism and qualitative determination of ferulate enzyme activity in one step, which shortens the screening time, and has high operability. The invention has highly actual application value in the production of fodder industry, paper making industry, spice industry and pharmacy material industry.

The invention relates to the technical field of reuse of discarded mushroom chaff after cultivation of edible fungi, in particular to a feed for Flammulina velutipes mushroom chaff and its production method and application. Flammulina velutipes fungus chaff feed comprises mixture a and mixture b with a mass ratio of 1000:1 to 1000:10; mixture a is composed of the following raw materials: enoki mushroom chaff powder, sweet potato dregs powder, rice bran, wheat flour, soybean meal, fish meal or meat Bone meal, salt; mixture b is composed of the following raw materials: cellulase, hemicellulase, Aspergillus niger, ligninase, cutinase, ferulic acid esterase, brown sugar. The production method of the enoki mushroom chaff feed is simple. The enoki mushroom chaff feed is used for cultivating barley worms, the time required for cultivating the barley worms is short, the body weight of the barley worms is large, and they lay many eggs. The cultured barley worms are added to conventional feed for feeding livestock, crabs, fish, soft-shelled turtles, eels, eels or frogs, and the fed animals grow significantly faster, and the yield is significantly increased, and the effect is very good.

The invention provides a recombinant strain of Kluyveromyces marx that can be used to prepare ferulic acid esterase AnfaeA, which clones the sequence-optimized Aspergillus niger ferulic acid esterase AnfaeA gene into an expression vector and transforms Kluyveromyces maximus The ferulic acid esterase production of the recombinant strain reaches 120000U / ml through high-density fermentation. The present invention also provides a method for preparing ferulic acid by using ferulic acid esterase to hydrolyze corn cob powder, using the synergistic effect of ferulic acid esterase AnfaeA and xylanase produced by the aforementioned recombinant strains to efficiently hydrolyze corn The core powder obtains ferulic acid. The ferulic acid esterase provided by the present invention can be used to biodegrade agricultural by-products such as corn cobs, rice bran and corn bran to prepare phenolic substances such as ferulic acid and p-coumaric acid, so it is widely used in food, papermaking, feed, medicine and other fields. All have wide application value, and meanwhile, the preparation method has simple process and high yield, and is very suitable for large-scale production.

The invention provides a method for detecting activity of ferulic acid esterase, which comprises the following steps of: mixing an enzyme solution to be detected with methyl ferulate solution to react, and calculating the enzyme activity. The invention can detect 12 samples on one plate with 96 holes with very small sample amount, thus simplifying the steps for detecting activity of ferulic acid esterase, greatly reducing the time, lowering working density and achieving accurate detection result and strong operability. The invention is suitable for batch activity detection of ferulic acid esterase.

The invention provides a method for detecting activity of ferulic acid esterase, which comprises the following steps of: mixing an enzyme solution to be detected with methyl ferulate solution to react, and calculating the enzyme activity. The invention can detect 12 samples on one plate with 96 holes with very small sample amount, thus simplifying the steps for detecting activity of ferulic acid esterase, greatly reducing the time, lowering working density and achieving accurate detection result and strong operability. The invention is suitable for batch activity detection of ferulic acid esterase.

The invention provides a strain of Aspergillus clavatus for producing feruloyl esterase and application thereof. The Aspergillus clavatus has the category name of Aspergillus clavatus fumigatus, is collected by China center for type culture collection, called CCTCC for short, at December 8, 2013, has the collection number of CCTCCNO: M2013641 and is applied to the production of feruloyl esterase. According to the strain provided by the invention, a method that feruloyl esterase is produced through fermenting by taking wheat bran as a fermentation substrate and bound-type phenolic and ferulic acids are obtained through rupturing ester bonds between the polysaccharide and lignin of plant cell walls and ferulic acid by the feruloyl esterase, is adopted, and the condition that microorganisms are short in growth cycle, are not restricted to climate and are easily subjected to large-scale production is utilized, so that a novel biological method for extracting bound-type phenolic acid substances, such as ferulic acid is provided.

The invention discloses a ferulic esterase-producing bacillus velei and application thereof, belonging to the technical field of microbes. The invention provides a strain of Bacillus velezensis (Bacillus velezensis) SK52.002. This Bacillus velezensis SK52.002 can highly produce ferulic acid esterase, which is embodied in the following steps: the Bacillus velezensis (Bacillus velezensis) ) SK52.002 was inoculated into the fermentation medium for fermentation, and after 16 hours of fermentation, the enzyme activity of ferulic acid esterase in the fermentation broth could be as high as 12mU / mL. Therefore, Bacillus velezensis SK52.002 in It has great application prospects in the production of ferulic acid esterase and ferulic acid.

The invention discloses a strain of Lactobacillus johnsonii with high ferulic acid esterase production and application thereof. The lactobacillus provided by the present invention is Lactobacillus johnsonii HCOM3 CGMCC No.15447. Proved by experiments: Lactobacillus johnsonii HCOM3 CGMCC No.15447 of the present invention can secrete ferulic esterase, has the function of degrading the dense network structure of plant cell wall, and can be used to improve the cellulose digestibility in the high-fiber silage raw material, Improve the palatability of silage raw materials, increase the utilization rate of silage raw materials and improve the growth performance of animals. At the same time, it also has the characteristics of fast acid production, fast growth, non-toxic to humans and animals, and no pollution to the environment. It not only improves the quality of agricultural and animal husbandry products and protects the environment. It is of great significance and also helps to promote the sustainable development of agriculture.

The invention relates to the technical field of probiotics, in particular to a Lactobacillus reuteri and an application thereof. The present invention provides Lactobacillus reuteri (Lactobacillus reuteri) SLZX19-12, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.20121. Lactobacillus reuteri SLZX19‑12 can colonize animals, and has strong acid resistance and bile salt resistance; it has good inhibitory effect on Escherichia coli and Salmonella; it can produce ferulic esterase, which can promote the digestion of fiber in the feed Release and digestion; feeding animals with Lactobacillus reuteri SLZX19‑12 as a bacterial agent can improve intestinal flora, enhance intestinal immunity, and improve intestinal health. The effect is safe and reliable.

The invention aims to provide a method for reforming heat stability of ferulic acid esterase A, constructing a mutant ferulic acid esterase A engineering bacterium and realizing high-efficiency heterologous expression of mutant ferulic acid esterase A. According to the space structure design mutant A126C and N152C derived from A.usamii E001 ferulic acid esterase A (AuFaeA), mutant ferulic acid esterase A: AuFaeA A126C-N152C is obtained, the mature peptide gene sequence thereof is SEQ ID NO:1, and C126 and C152 can form one disulfide bond. Experimental results indicate that the heat stability of the enzyme is obviously improved after mutation, thus providing a new technological path for reforming of other enzymes. As a heat-resistant enzyme preparation, the ferulic acid esterase has great industrial production potential and economic value.

The invention discloses a method for preparing a complex enzyme preparation of heat-resistant ferulic acid esterase and cellulose by fermenting trichoderma atroviride through an airlift fermentation tank. According to the method, the trichoderma atroviride is taken as a production strain, wherein the method comprises the following steps: preparing a fermentation strain, preparing a spore suspension, preparing a seed solution, carrying out liquid fermentation through a shaking flask, preparing the complex enzyme preparation and preparing the complex enzyme preparation of heat-resistant ferulic acid esterase and cellulose. The prepared complex enzyme preparation is high in activity, soluble in water, free of peculiar smell, high in heat stability and high in catalytic activity, and has a good prospect of industrialization application.

The invention discloses a method for immobilizing ferulic acid esterase with magnetic nanomaterials, which comprises the preparation of Fe2O3 immobilized carrier magnetic nanomaterials; mixing the immobilized carrier magnetic nanomaterials with ferulic esterase solution; and preparing on magnetic equipment Immobilized ferulic acid esterase. The preparation process of the invention is simple, the reaction conditions are mild, and the utilization rate of repeated regeneration is high. The prepared immobilized ferulic acid esterase maintains more than 90% of the equivalent free enzyme activity, and the thermal stability is improved. The enzyme activity of ferulic acid esterase can preserve more than 80%.