22 results about "Ferulic acid esterase" patented technology

The invention relates to a high-temperature-resistant feruloyl esterase liquid as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) constructing a reaction system: suspending recombinant microorganism thalli for expressing high-temperature-resistant feruloyl esterase in a buffer solution with the pH value of 7.0-9.0; (2) ultrasonic cracking and thermal purification: heating the reaction system to 70-85 DEG C under the condition of ultrasonic crushing, and keeping the temperature for 1-8 hours; carrying out structural destruction on the microbial thalli by utilizing ultrasound so as to release intracellular high-temperature-resistant feruloyl esterase, and carrying out denaturation and precipitation on host impure protein by utilizing the thermal stability difference between the high-temperature-resistant feruloyl esterase and the host impure protein; after the high-temperature-resistant feruloyl esterase is subjected to heat treatment in the step (2), the catalytic activity is kept at the temperature of 60 DEG C or above; and (3) obtaining an enzyme solution, namely centrifuging the reaction body fluid subjected to the ultrasonic treatment and the heat treatment in the step (2), and collecting supernate, so as to obtain the high-temperature-resistant feruloyl esterase solution.

The invention belongs to the technical field of microorganisms, and provides a high-yield feruloyl esterase plant lactobacillus and application thereof. The bacterial strain is lactobacillus plantarum Fs6, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.36264, and the preservation date of the bacterial strain is October 20, 2025. According to the present invention, the fermentation performance of the dough can be significantly improved with the plant lactobacillus, and the good synergistic effect is represented during the co-fermentation with the yeast; and a good synergistic effect is shown during co-fermentation with yeast. When the mass concentration of bile salt is 0.3 and 0.6 g / L, the survival rate is relatively good, which shows that the lactic acid bacteria Fs6 has a certain probiotic function. The self-aggregation force (32.77%) and the hydrophobicity (32.43%) of the Fs6 are relatively low, and the adhesion of the Fs6 is relatively poor. And combined phenolic acid in the grains can be effectively released, so that the nutritional value of the grains can play a better health role in a human body.

This invention provides a feed additive and compound feed for preventing and treating coccidiosis in broilers, comprising the following components: *Kluyveromyces martensii*, ferulic acid esterase, aminopeptidase, and glucanase. Preferably, the mass percentage of *Kluyveromyces martensii* is 30-40%; the mass percentage of ferulic acid esterase is 15-28%; the mass percentage of aminopeptidase is 15-25%; and the mass percentage of glucanase is 18-25%. Based on extensive research, this invention has determined that this combination has a significant effect on coccidiosis control in broilers, effectively improving broiler production performance indicators, increasing economic benefits, and reducing the use of anticoccidial drugs.

The invention belongs to the technical field of bioengineering, and particularly relates to myrobalan fruit nano-vesicles, a preparation method and application of the myrobalan fruit nano-vesicles in skin aging resisting products. The preparation method comprises the following steps: firstly, carrying out enzymolysis on a myrobalan fruit raw material by adopting a compound enzyme preparation containing feruloyl esterase, and adding sodium alginate and a calcium chloride solution into enzymatic hydrolysate after enzymolysis to carry out precipitation treatment on nano-vesicles; and finally, treating the precipitate by adopting a sodium carbonate or sodium bicarbonate solution, fully stirring, centrifuging, and collecting the supernatant, thereby obtaining the purified product of the myrobalan fruit nano-vesicle. The preparation method of the myrobalan fruit nano-vesicle is efficient and low in cost, the content of miRNA in the obtained nano-vesicle is high, animal experiments show that the nano-vesicle can reduce the content of IL-6 inflammatory factors in experimental animal skin tissue and reduce collagen loss caused by ultraviolet rays, and the nano-vesicle has remarkable anti-aging and light aging relieving effects and can be used for preparing the nano-vesicle. The method can be applied to functional skin care products.

The application discloses a silage fermentation accelerator based on enzyme and bacteria cooperation and a preparation method thereof, and belongs to the technical field of feed preparation, and comprises the following steps: S1: taking sterilized selenium-rich wheat bran and sterilized yeast hydrolysate as carriers, mixing cellulase, xylanase, ferulic acid esterase, phytase and tannase to prepare a composite carrier enzyme; mixing composite bacteria powder and selenium-rich yeast powder to prepare an active mixture; mixing xylo-oligosaccharide, betaine and vitamin E to prepare active material to be embedded; S2: taking chitosan and sodium alginate as a skeleton, mixing N-isopropyl acrylamide, ammonium persulfate and N,N'-methylene bisacrylamide, and in-situ polymerizing to construct a temperature-sensitive hydrogel, and then performing dialysis, freeze-drying and grinding to prepare a temperature-sensitive embedding liquid; S3: rotating and shearing the active material to be embedded and the temperature-sensitive embedding liquid to prepare embedding suspension liquid to be sprayed, and then performing continuous spray embedding to prepare temperature-changing microcapsule particles, namely the silage fermentation accelerator based on enzyme and bacteria cooperation; and the application promotes silage fermentation.

The invention provides a high temperature resistant feruloyl esterase and an application thereof. The feruloyl esterase wild type has a remarkable high-temperature-resistant characteristic and still shows relatively high thermal stability under the condition of 95 DEG C, and the thermal inactivation half-life period of the feruloyl esterase wild type can reach 5h. On the basis, after the feruloyl esterase mutant obtained through structural rationality modification continuously reacts for 5 h at the temperature of 75 DEG C, floccule precipitation is not observed, and stable catalytic activity is kept. In the application, the feruloyl esterase mutant disclosed by the invention and commercial xylanase have a synergistic effect, so that the release of bound ferulic acid in starch-removed wheat bran can be effectively promoted. The invention provides a novel enzymology tool for efficiently and quickly releasing the plant-derived ferulic acid under a high-temperature condition.

This invention relates to a method for improving the intestinal tolerance of wheat bran dietary fiber based on synergistic modification by microorganisms and enzymes. The method includes ultra-fine grinding and high-pressure moist heat activation of wheat bran; multi-enzyme synergistic microbial stepwise treatment, first using high-temperature amylase and neutral protease to remove interfering components such as starch and protein, then using laccase to degrade lignin, resulting in a looser structure; subsequently, phytase efficiently degrades phytic acid, Aspergillus nidulan degrades the side chains of arabinogalactan, and snail enzyme and ferulic acid esterase work synergistically to convert insoluble arabinoxylan into soluble dietary fiber and release phenolic antioxidants. This method increases the soluble dietary fiber content of wheat bran by 2 times, reduces phytic acid to 10 mg / g, increases free phenolic acid content by 40%, achieves a DPPH free radical scavenging rate of 60%, significantly enhances antioxidant activity, and significantly reduces IgG levels in mouse models compared to those consuming unmodified wheat bran, thus improving intestinal tolerance and probiotic function; the product can be used as a high-quality functional food ingredient.

The invention relates to a pretreatment method of xylooligosaccharide preparation raw materials. The pretreatment method comprises the following steps: step 1, preparing a substrate; step 2, removing starch from amylase; step 3, removing protein by protease; step 4, complex enzyme ultrasonic-assisted cell wall structure loosening; according to the pretreatment method for the raw materials for preparing the xylooligosaccharide, the enzymolysis process is carried out in a water phase, no strong acid or strong alkali is needed, no pollution is discharged, and the green chemistry principle is met; in addition to cellulose and hemicellulose, the components with high proportions in the bran are starch and protein, and the starch and the protein are removed before the xylan is exposed, so that system impurities can be effectively reduced, and convenience is provided for xylan extraction and xylooligosaccharide separation and purification; the feruloyl esterase and the lignin degrading enzyme are compounded. The xylan and the xylanase cooperate with each other, the cross-linked structure of cell walls is collapsed from different dimensions, the accessibility of the xylan is greatly improved, and a solid foundation is laid for subsequent enzymolysis.

The invention relates to the technical field of microbial application and bioengineering, and discloses an aspergillus awamori SNJ-2F capable of simultaneously producing feruloyl esterase, cellulase and xylanase at high yield, which is separated from Shennongjia high-temperature yeast for making hard liquor and preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M20252024. The invention also discloses a screening method of the strain, a bran seed preparation process and a method for producing ferulic acid by performing enzymolysis on starch-removed wheat bran with a bran seed crude enzyme. The strain disclosed by the invention is strong in enzyme system synergy, green in process and low in cost, can be used for ferulic acid preparation, white spirit / soy sauce brewing and feed industry, and has a wide application prospect.

The application belongs to the technical field of bioengineering, and particularly relates to a Terminalia chebula Retz. fruit nano vesicle, a preparation method thereof and application of the nano vesicle in anti-skin aging products. In the application, a composite enzyme preparation containing ferulic acid esterase is used to perform enzymolysis on Terminalia chebula Retz. fruit raw materials, sodium alginate and a calcium chloride solution are added to the enzymolysis liquid after the enzymolysis, and the nano vesicle is subjected to precipitation treatment; finally, a sodium carbonate or sodium bicarbonate solution is used to treat the precipitate, the mixture is fully stirred, centrifuged, and the supernatant is collected to obtain a purified product of the Terminalia chebula Retz. fruit nano vesicle. The preparation method of the Terminalia chebula Retz. fruit nano vesicle provided by the application is efficient and low in cost, the nano vesicle obtained has a high miRNA content, animal experiments show that the nano vesicle can reduce the content of IL-6 inflammatory factors in the skin tissue of experimental animals, reduce the loss of collagen caused by ultraviolet rays, has significant anti-aging and light-aging alleviating effects, and can be applied to functional skin care products.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BccFae3074, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BcFae4711, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

This invention discloses an enzymatic extraction and fractional purification method for chlorogenic acid from coffee, belonging to the field of natural product extraction technology. The method employs a two-stage synergistic enzymatic hydrolysis strategy using cellulase, pectinase, and ferulic acid esterase. In the first stage, the cell wall skeleton is destroyed to release free chlorogenic acid. In the second stage, ester bonds are directionally cleaved to release bound chlorogenic acid. The enzymatic hydrolysate is then eluted with a four-stage polar gradient through a macroporous adsorption resin to achieve fractional purification. This method can simultaneously obtain products of three purity levels, with the pure chlorogenic acid component achieving a purity of over 95% and a total extraction yield of over 75%.

The invention relates to the field of biotechnology and industrial enzyme preparations, and particularly discloses composite cellulase for lignocellulose residue enzymolysis and application of the composite cellulase. The compound cellulase is an enzyme system which is synergistically designed aiming at an interlaced structure of cellulose and hemicellulose in lignocellulose residues, and at least comprises incision beta-1, 4-glucanase, excision beta-1, 4-glucanase, beta-glucosidase and xylanase, and auxiliary enzyme components such as beta-glucanase, mannase, arabinosidase and / or feruloyl esterase can be further included according to the structural characteristics of different lignocellulose residues. According to the method disclosed by the invention, a plurality of compound enzyme systems adaptive to different raw material structural characteristics are constructed, so that efficient synergistic degradation of lignocellulose residues such as straws, bagasse, manioc waste and vinasse is realized, the enzymolysis efficiency of cellulose and hemicellulose is improved, inhibition of accumulation of intermediate products on an enzymolysis reaction is reduced, and the quality of the lignocellulose residues is improved. And the method is suitable for industrial application under medium-high solid content conditions. The composite cellulase can be used for saccharification and fermentation pretreatment of lignocellulose residues and has the advantages of being high in raw material adaptability, high in enzymolysis efficiency and wide in application range.

The present application relates to the technical field of microbial application, and specifically discloses a mucilaginibacter sp. Q2 producing ferulic acid esterase, a bacterial suspension, a preparation method and an application, wherein the mucilaginibacter sp. is preserved in the China Center for Type Culture Collection on January 29, 2024, and the preservation number is CCTCC NO: M 2024254. The mucilaginibacter sp. Q2 obtained by separation has the characteristic of producing ferulic acid esterase.

The invention discloses a method for producing feruloyl esterase by utilizing bacillus altitudinis and application of the feruloyl esterase. The invention belongs to the technical field of microorganisms, and provides bacillus altitudinis ZH-1 capable of producing feruloyl esterase, the bacillus altitudinis ZH-1 can produce feruloyl esterase at high yield, and the bacillus altitudinis ZH-1 is specifically embodied in that the feruloyl esterase activity in fermentation liquor can reach 254U / L after the bacillus altitudinis ZH-1 is inoculated into a fermentation culture medium for fermentation for 72 hours, so that the feruloyl esterase activity can reach 254U / L, and the feruloyl esterase activity can reach 254U / L; the bacillus altitudinis ZH-1 has a huge application prospect in the production of feruloyl esterase and ferulic acid.

The application discloses a Chinese herbal medicine enzyme composition and application thereof; the composition comprises a mesophilic alpha-1,4-amylase, a pullanase, a ferulic acid esterase, a glucose oxidase, a catalase and a Boerhaave dispersing composite preparation; each component in the composition has a strong synergistic combination effect, can synergistically improve a feed nutrient digestion rate of a Langde goose, improve growth performance, increase an unsaturated fatty acid content in a Langde goose liver and improve goose liver quality, and increase breeding benefits.

The invention belongs to the technical field of papermaking, and particularly relates to a compound enzyme preparation for pulping arrowhead pulp and application of the compound enzyme preparation. The complex enzyme preparation provided by the invention comprises xylanase and lysing polysaccharide monooxygenase, and further comprises mannase or feruloyl esterase. The compound enzyme preparation can remarkably improve the pulping effect of the arrowhead pulp, reduce the pulping energy consumption and increase the kink index, the water retention value and the brooming degree of fibers. After neosinocalamus affinis chemithermomechanical pulp and neosinocalamus affinis chemical pulp treated by the compound enzyme preparation are subjected to sheet making to form paper, the burst index and tensile strength of the paper are improved, and the paper shows excellent physical properties.

The invention discloses a method for flexibly and moderately processing edible oil through biological enzymolysis, and belongs to the technical field of edible vegetable oil processing. The method sequentially comprises the steps of cleaning and color sorting, ozone sterilization, moderate conditioning, biological enzymolysis treatment, moderate aroma enhancement, squeezing at proper temperature, moderate refining, filtering at proper temperature and preservation at proper temperature. The method is characterized in that ultrasonic pretreatment is innovatively introduced after tempering and cooperates with an enzymolysis process that cellulase and feruloyl esterase are compounded according to the mass ratio of 1: 1, and then two-waveband infrared is adopted for aroma enhancement. According to the method, traditional chemical refining is abandoned, the problems of nutrition loss and flavor damage caused by excessive processing are effectively solved, the oil pressing rate and the oil oxidation stability are remarkably improved, active nutritional ingredients such as polyphenols such as vitamin E and squalene are reserved to the maximum extent, and high-valued green processing of oil plants is achieved.

The invention provides a plant lactobacillus for producing feruloyl esterase and application thereof, and relates to the technical field of microorganisms and food processing, the plant lactobacillus for producing feruloyl esterase is plant lactobacillus K33 and is preserved in the China Center for Type Culture Collection (CCTCM), and the preservation number is CCTCCM20251679. After the lactobacillus plantarum K33 is cultured in a fermentation culture medium for 48 hours, the content of the ferulic acid released by the lactobacillus plantarum K33 is 0.28 + / -0.01 mg / mL, and the bioactivity of the lactobacillus plantarum K33 is utilized, so that the release of the combined ferulic acid can be effectively promoted, and the content of the free ferulic acid in the fermentation liquid is increased, thereby obviously improving the absorption and utilization of the ferulic acid by the body. The plant lactobacillus K33 is used for producing the whole citrus pulp fermentation liquor, the content of ferulic acid and other organic acids in the fermentation liquor can be effectively increased, and an effective biological way is provided for increasing the content of free ferulic acid in fermented food.

The application provides high-SDF wheat bran powder and a preparation method and application thereof. The steps are as follows: S1. high-quality wheat bran is taken, washed, dried, ultra-finely pulverized and sieved to obtain wheat bran powder; S2. water is added to the wheat bran powder, cellulase is added, ultrasonic treatment is carried out, and enzyme hydrolysis is carried out, 80 DEG C short-time enzyme inactivation is carried out, and the fermentation substrate is obtained after cooling; S3. the water content of the substrate is adjusted, and constant-temperature fermentation is carried out in stages at 37 DEG C: stage one is aerobic fermentation by inoculating Bacillus natto; stage two is anaerobic fermentation by adding sterile water to adjust the water content, introducing nitrogen, inoculating Lactobacillus plantarum and adding food-grade ferulic acid esterase; S4. the fermentation product is subjected to vacuum freeze-drying, ultra-fine pulverization and sieving, and the high-SDF wheat bran powder is obtained. The application breaks through the bottleneck of the disconnection between pretreatment and fermentation, low enzyme utilization rate and non-targeted product conversion in the existing process by using the mild enzyme-preserving pretreatment-bacteria-enzyme synergistic stage-by-stage fermentation technology, realizes efficient value-added modification of wheat bran resources, and expands the application scene of the wheat bran in low GI functional food.

The application belongs to the technical field of biotechnology, and particularly relates to a high-efficiency expression of a Kluyveromyces marxianus strain and application thereof. The Kluyveromyces marxianus strain is obtained by using CRISPR / Cas9 gene editing technology to knock out genes in a protein synthesis metabolic pathway, a secretion and transport pathway, a cytoskeleton synthesis or an intercellular information transmission pathway in a genome of the Kluyveromyces marxianus, and taking the Kluyveromyces marxianus FIM-1 in which a key gene URA3 of a uracil synthesis pathway is knocked out as a starting strain. A FIM-1 ura3Delta mnn11Delta is screened and obtained, and the alpha-galactosidase secretion expression level can be increased by more than 3 times. The Kluyveromyces marxianus strain can be applied to high-efficiency preparation of biological enzymes such as alpha-galactosidase, ferulic acid esterase, chitosanase, saccharifying enzyme, xylanase, mannanase, cellulase and beta-amylase, and has high industrial application value.