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Lactobacillus plantarum capable of producing ferulic acid esterase

A technology of Lactobacillus plantarum and ferulic acid esterase, applied in the direction of Lactobacillus, bacteria used in food preparation, bacteria, etc., can solve the problems of limiting cellulose utilization and degradation, achieve wide application value, improve fermentation quality, improve The effect of digestibility

Inactive Publication Date: 2018-11-13
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the cell wall of plants, ferulic acid monomers and various dimers are cross-linked with hemicellulose and lignin through ester bonds, which strengthens the three-dimensional polysaccharide network structure, and limits the utilization of cellulose and microorganisms. For the degradation of plant fiber materials

Method used

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  • Lactobacillus plantarum capable of producing ferulic acid esterase
  • Lactobacillus plantarum capable of producing ferulic acid esterase
  • Lactobacillus plantarum capable of producing ferulic acid esterase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening experiment of strains producing ferulic acid esterase.

[0029] 1. Activate the lactic acid bacteria 2-3 times, streak on the MRS solid medium, and culture for 2 days at 37°C.

[0030] 2. Pick a single colony on the MRS solid medium without glucose, and use ethyl ferulate as the sole carbon source. Add 0.3ml of ethyl ferulate solution to each plate (dissolve ethyl ferulate in Dimethylformamide was made into a solution with a mass-volume ratio of 10%), cultured at 30°C for 72 hours, and observed whether a transparent bacterial circle appeared on the plate.

[0031] 3. It can be preliminarily judged to produce ferulic acid esterase. The tested Lactobacillus plantarum FE1 produced transparent circles.

Embodiment 2

[0032] Example 2: Crude enzyme solution for liquid culture and enzyme activity analysis and determination.

[0033] 1. After the initial screening, the Lactobacillus plantarum FE1 strain was cultured in MRS liquid medium at 37°C overnight, centrifuged at 3000 rpm for 5 min, and the bacteria were collected.

[0034] 2. Wash the bacteria 3 times with 0.85% normal saline and resuspend in deionized water.

[0035] 3. Take bacteria suspension (fixed bacteria number 1×10 9 cfu / ml) was inoculated into the medium containing methyl ferulate, 100ml medium was added with 5ml methyl ferulate (1% W / V dimethylformamide), the inoculation amount of the strain was 2% (V / V), ferment. The fermentation conditions were 37°C, 48h, and the oscillation frequency was 120rpm.

[0036] 4. Pipette 9ml of fermentation broth into a 10ml centrifuge tube, centrifuge at 10000rpm for 10min, the supernatant is the crude ferulic acid esterase enzyme solution, which is used for enzyme activity determination.

[0037] 5....

Embodiment 3

[0045] Example 3: Analysis and determination of physicochemical properties of FAE-producing Lactobacillus plantarum FE1.

[0046] 1. Sugar fermentation, arginine ammonia production, nitrate reduction and glucose gas production.

[0047] Using bacteria micro biochemical reaction tube.

[0048] The strain is activated for 2-3 times, streaked and cultured for 2-3 days, a single colony is picked with an inoculation loop and placed in a biochemical tube, sealed with sterilized glycerin, and incubated at 37°C for 2-3 days to observe its discoloration.

[0049] Table 2 The utilization ability of the lactic acid bacteria of the present invention on the sugar source.

[0050]

[0051] Note: "+" means using the sugar; "—" means not using the sugar. CGMCC, China Microorganism Collection. C-LP, the standard strain of Lactobacillus plantarum. LP, Lactobacillus plantarum.

[0052] 2. Strain growth characteristics under different temperature conditions.

[0053] After activating the lactic acid bacte...

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Abstract

The invention discloses a lactobacillus plantarum strain separated from silage and used for producing ferulic acid esterase. The lactobacillus plantarum strain has the preservation number of CGMCC12955, has the ability to produce the ferulic acid esterase, and can maintain good stability at the pH of 5-7 and the temperature of 25-50 DEG C, optimally, at the pH of 6.4 and the temperature of 37 DEGC. The lactobacillus plantarum strain can be applied to preparation of silage additives and preparation of health care foods and medicines.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to the field of development and application of lactic acid bacteria, particularly a strain of plant lactobacillus producing ferulic acid esterase. Background technique [0002] Ferulic acid (C 10 H 10 O 4 ), the chemical name is 4-hydroxy-3-methoxycinnamic acid, which is one of the most widely used light-based cinnamic acid components in the plant kingdom, because it can remove active oxygen free radicals, absorb ultraviolet rays, inhibit pigment production and It has anti-inflammatory effects and is widely used in medicine, cosmetics and chemical industries. In addition, in the cell wall of plants, ferulic acid monomers and various dimers are cross-linked with hemicellulose and lignin through ester bonds, which strengthen the three-dimensional polysaccharide network structure and limit the utilization of cellulose and microorganisms. For the degradation of plant fiber materials. [0003] Feru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/25
CPCA23K30/18C12N1/20C12R2001/25C12N1/205A23V2400/169
Inventor 郭旭生丁武蓉柯文灿许冬梅张萍
Owner LANZHOU UNIVERSITY
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