Microbial inoculum and application thereof in improvement of facility agriculture

A microbial inoculum, a technology for facility agriculture, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc.

Active Publication Date: 2012-12-19
宋彦耕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these existing technologies have not studied the pesticide spectrum that can effectively eliminate pesticide residues, and there is no inspiration to improve plant stress resistance and reduce the dead plant rate.
[0004] However, the inventors found in production that too many types of microbial strains will bring about complicated preparation process and high preparation cost
However, a variety of microorganisms form an extremely complex symbiotic relationship, and the growth environment requirements of different microorganisms are also very different. Some of them can even secrete antibiotics and require the cooperation of other strains to produce synergistic tolerance. Deconstruction is not explicitly taught by prior art
Therefore, rashly reducing the types of strains will lead to an imbalance of strains in the entire bacterial preparation. For example, the inventors have found that if Cellulomonas is removed, Lactobacillus, Streptomyces and Rhizopus in the bacterial preparation will basically not be able to survive. (It may be due to the lack of the growth microenvironment formed by Cellulomonas, and the ability to synergistically resist antibiotics may also be lost), and the organic fertilizer produced by final fermentation cannot meet the national standard; Miscellaneous bacteria are produced, thereby inhibiting the growth of bacteria added to the bacterial agent, causing the quality of the bacterial agent between batches to be extremely unstable, so that it cannot be promoted as a commodity at all, and so on, etc.
[0005] For this reason, the present inventor combines some luck through long-term arduous research, has found that if adopt Cellulomonas fabia (Cellulomonasfabia ) this specific bacterial species can not add Aspergillus niger and Bacillus subtilis in the bacterial agent, thereby reducing the production cost, but can still maintain the stability of the bacterial agent to be suitable for industrial production, and make the organic fertilizer produced by fermentation fully capable of In line with national standards, what is even more surprising is that the products produced by the fermentation of this bacterial agent can not only improve soil fertility, reduce or eliminate pesticide residues, but also improve plant stress resistance and reduce dead plant rates. Such characteristics make this fermentation The product is especially beneficial to facility agriculture (because it is implemented in a closed environment such as a greenhouse, pesticide residues are not easy to dissipate; and the selected plant varieties are often poor in stress resistance and resistance to diseases and insect pests in order to pursue yield and quality, and the latter can be selected. to solve (however, the type and amount of pesticides will be increased), while the former has no good solution for a given plant variety)

Method used

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  • Microbial inoculum and application thereof in improvement of facility agriculture
  • Microbial inoculum and application thereof in improvement of facility agriculture
  • Microbial inoculum and application thereof in improvement of facility agriculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The production of embodiment 1 microbial bacterial agent

[0049] The present embodiment uses the following strains to prepare microbial inoculum: (1) Cellulomonas faeci ( Cellulomonas fabia ), (2) Bacillus stearothermophilus ( Bacillus sterothermophilus ), (3) Lactobacillus casei ( Lactobacillus casei ), (4) Methanobacter ants ( Methanobacterium formicium ), (5) Methanobacter ruminantum ( Methanobacterium ruminantium ), (6) Thiobacillus thiooxidans ( Thiobacillus thiooxidans), (7) Thiobacillus ferrooxidans ( Thiobacillus ferrooxidans ), (8) Bacillus licheniformis ( Bacillus licheniformis ), (9) Bacillus polymyxa ( Bacillus polymyxa ), (10) Streptomyces thermophilus ( Streptomyces thermophilus ), (11) common high temperature actinomycetes ( Thermoactinomyces vulgaris ), (12) Thermomonospora curved ( Thermonospora curvata ), (13) brown nitrogen-fixing bacteria ( Azotobacter vinelandii ), (14) Nitrosomonas europeanum ( Nitrosomonas europeae ), (...

Embodiment 2

[0052] Example 2 Production of Organic Fertilizer

[0053] Take cow dung with a water content of 65% (weight ratio) (if the water content is insufficient, you can add water) and crushed rice bran with particles smaller than 5mm, mix them evenly at a mass ratio of 85:15, and make the mixed cow dung and rice bran There is no agglomeration above 40mm.

[0054] Then, the mixture of 1 ton of above-mentioned cow dung and rice bran is mixed with the microbial inoculant produced in 1kg of Example 1, and heaped into the fermenter to form a compost heap. During the compost heap placement period, along with the fermentation process, the compost heap can naturally heat up. This fermentation heating process usually takes 2-3 days, and the compost heap can be turned 1-2 times during this period. Then, when the temperature of the compost heap rises to 65°C, turn on the turner to turn the compost heap to dissipate the heat generated by fermentation to ensure that the temperature does not e...

Embodiment 3

[0056] Example 3 Effects of Organic Fertilizers on Fruit Growth and Yield

[0057] This embodiment describes that the inventor entrusted Beijing Wandeyuan Agricultural Science and Technology Development Co., Ltd. to carry out in Beijing Changping District Facility Strawberry Park (Xiaotangshan Wande Strawberry Garden) from August 1, 2011 to March 1, 2012 Effect test of strawberry planting application.

[0058] 1. Test materials and design

[0059] 1.1 Test material

[0060] Bio-organic fertilizer: the organic fertilizer produced by the same method as in Example 2

[0061] Strawberry Varieties: Imperial Use, Wande No. 1 (Dianxue).

[0062] 1.2 Experimental design

[0063] The experiment has two treatments:

[0064] ① Greenhouses numbered A15, A16, and A17 were set as the control, numbered CK; the base fertilizer of the control and test sheds used 2.5 tons of puffed chicken manure per mu as the base fertilizer, and the control shed applied 100kg / mu of Stanley compound fer...

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Abstract

The invention provides a viable microbial composition for fermenting and producing a facility agriculture ameliorant, which is characterized in that the microbial composition is composed of Cellulomonasfabia, Bacillus stearothermophilus, Lactobacilli, methanobacteria, thiobacilli, Bacillus licheniformis, Bacillus polymyxa, streptomycete, Thermoactinomyces, Thermomonospora, azotobacter, Nitrosomonas, nitrobacter, rhizobia, Aspergillus oryzae, saccharomyces, penicillium, Humicola, Rhizopus and mycorrhiza fungi. In addition, the invention also provides a facility agriculture ameliorant which is fermented and produced by the microbial composition, application, a method and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation. Specifically, the invention relates to a microbial composition, which reduces the number of strains and reduces the production cost without substantially reducing the stability and adaptability of the microbial agent produced therefrom, but The organic fertilizer produced by fermentation can still fully meet the national standards, and it also brings about the improvement of plant stress resistance. In addition, the present invention also relates to the improved agent for facility agriculture produced by the fermentation of the above-mentioned microbial composition, as well as its application and method. Background of the invention [0002] At present, as people pay more and more attention to health and food safety, the quality requirements of agricultural products are getting higher and higher. It is hoped that on the basis of increasing the production and income of agricultural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/18C12N1/20C05F15/00C05F17/00C12R1/01C12R1/07C12R1/225C12R1/10C12R1/12C12R1/465C12R1/065C12R1/41C12R1/69C12R1/865C12R1/80C12R1/645C12R1/845
CPCY02E50/30Y02W30/40
Inventor 宋彦耕
Owner 宋彦耕
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