33 results about "Cellulomonas sp." patented technology

The invention discloses a pyridine-degrading bacterium as well as a composite bacterial agent, a preparation method and uses thereof. The newly-separated Rhodococcus pyridinovora KT-J002 CGMCC No.2789 and Cellulomonas sp KT-J007 CGMCC No.2788 not only can effectually degrade the high concentration pyridine, but also has tolerance or degradation ability for toxic substances such as benzene, phenol, dimethylbenzene, quinoline, cyanide and the like; the composite bacterial agent composed of Rhodococcus pyridinovora KT-J002 CGMCC No.2789, Cellulomonas sp KT-J007 CGMCC No.2788, Paracoccus d enitrificans W12 CGMCC No.1673, Micrococcus luteus ATCC 49442 and Arthrobacter crystallopoietes ATCC 15481 is used for environmental improvement, can effectually degrade the pyridine and is particularly suitable for the biological treatment of coking waste water.

The invention relates to the biological fermentation technology, in particular to a compound microbial starter, and a method which uses the compound microbial starter to produce high-energy protein biological feed. The compound microbial starter selects cellulomonas sp dinitrogen, cellulomonas flavigena, condensate bacillus, lactobacillus acidophilus, bacillus subtilis, lactobacillus starch, compound enzyme bacteria, propionic acid bacteria, candida yeast, saccharomyces diastaticus, saccharomyces cerevisiae, as well as aroma-producing yeast as the bacteria sources, and makes use of the modern biotechnology to prepare various microbial agents; the microbial agents are then treated with anaerobic fermentation with the discarded sweet corn cobs, corn stalks, pea skins, abandoned vegetables and other discarded crops, so as to obtain the high-energy protein biological feed required in livestock and poultry breeding industry. The invention solves the problem of low utilization rate of crop resources; and the high-energy protein biological feed can improve the palatability of feed, increase the enzyme content of the feed, provide an effective way for developing the animal husbandry and solving the food conflicts between people and livestock, as well as reduce the pollution to the environment caused by the traditional crop waste burning.

The invention relates to a household sludge reduction compound microbial preparation as well as preparation and application methods thereof, belonging to the field of microbial application. Based on the total bacteria count, the household sludge reduction compound microbial preparation comprises 10-20% of Celloulonmonassp., 10-20% of Serratiasp., 30-60% of Bacilluspumilus and 20-30% of Rhodotorulamucilaginosa, wherein the Celloulonmonassp. is selected from at least one of CCTCCAB209393 and YMCCKMS3990, the Serratiasp. is selected from at least one of ACCC02490 and CICC20143, the Bacilluspumilus is ACCC10113 and the Rhodotorulamucilaginosa is CICC31192. The microbial preparation and the preparation method provided by the invention are simple and feasible, economic and reasonable and free from secondary pollution to the environment.

The invention discloses a straw degradation acidification microbial inoculum and a manufacturing method thereof. Active ingredients of the composite microbial inoculum for degrading straw are cellulomonas flavigena, clostridium cellulolyticum, bacillus subtilis and bacillus cereus. The microbial inoculum breaks through the limitation that fungi and lytic enzymes thereof cannot quickly and efficiently decompose natural cellulose, a key pretreatment technology is provided for straw biogas fermentation, and the straw degradation acidification microbial inoculum has wide application prospect in the field of preparation of biogas from lignocellulose raw materials such as the straw.

The invention provides a live microbial composition for fermentation treatment of cultivating wastes. The composition is characterized by consisting of bean fiber monad, bacillus stearothermophilus, lactobacillus, methanobacteria, thiobacillus, bacillus licheniformis, bacillus polymyxa, streptomycete, thermoactinomycete, thermomonospora, nitrogen-fixing bacteria, nitrated monad, nitrobacteria, rhizobium, Aspergillus oryzae, yeasts, moulds, humicola, rhizopus and glomus mosseae. In addition, the invention further provides an organic fertilizer generated by virtue of fermentation of the microbial composition as well as an application and a method thereof. In production of the organic fertilizer, cultivating wastes such as animal wastes are objectively treated.

The invention discloses a fermentative bacteria solution for peanut vines and a peanut vine fermentation feed, which belong to the technical field of fermentation feeds. The fermentative bacteria solution for peanut vines is composed of the following components in parts by weight: 30-40 parts of a bacteria solution A and 10-15 parts of a bacteria solution B, every 40g of the bacteria solution A contains cellulomonas (1*10<9>-4*10<9> CFU), butyrivibrio fibrisolvens (1.5*10<9>-5*10<9> CFU), saccharomyces cerevisiae (1.68*10<9>-4.32*10<9> CFU), bacillus subtilis (2.25*10<10>-6.67*10<10> CFU), corn steep liquor (1-3g by dry matters), and the balance of water and necessary microbial matrixes; and every 10g of bacteria solution B contains lactic acid bacteria (0.22*10<10>-0.96*10<10> CFU), corn steep liquor (0.47-1.35g by dry matters), whole milk powder (0.67-2g), and the balance of water and necessary microbial matrixes. A peanut vine fermentation feed prepared by using the fermentative bacteria solution is prepared from the following components in parts by weight through mixing and fermenting: 45-55 parts of peanut vine powder, 5-8 parts of corn flour, 2-3 parts of soybean meal powder, 1-2 parts of urea, 1-2 parts of calcium sulphate and 40-55 parts of the fermentative bacteria solution.

The invention discloses an efficient composite organic matter decomposing inoculant capable of accelerating decomposition of organic matters, belonging to the technical field of microbes. The efficient composite organic matter decomposing inoculant is prepared by mixing the following components in percentage by volume: 30% of Cellulomonas flavigena zymophyte solution, 30% of Bacillus stearothermophilus zymophyte solution and 40% of Bacillus subtilis zymophyte solution. The total viable count of the three strains is 1 billion/ml, and the microbial inoculant type is liquid. The strains are respectively cultured in a fermentation tank without foreign microbes, and then compounded to obtain the efficient composite organic matter decomposing inoculant. By matching the three strains, various organic matters can be quickly degraded and converted into available organic fertilizers containing multifunctional probiotics within 15 days or so, and thus, the invention overcomes the defect that the traditional degradation needs more than 3 months.

The invention discloses a method for evaluating microbial powder promoting degradation of garden waste. In the method, the microbial powder is inoculated to the landscaping waste, culture is performedat the constant temperature of 30+/-1 DEG C, the mass change of degradation residuums of the landscaping waste is periodically measured, and the degradation efficiency of the microbial powder to thelandscaping waste is evaluated based on the degradation weight loss rate; when culture is performed for 15 days, the degradation weight loss rate of the microbial powder to the landscaping waste is not lower than 30%; the microbial powder is formed by mixing cellulomonas flavigena thalli, an adsorptive carrier and a protective agent in the mass ratio of 1:(0.5-1.5):(0.5-1.5). The adsorptive carrier is selected from dextrin, diatomaceous earth, talcum powder and calcium carbonate; a wetting agent is selected from Tween 80, polyethylene glycol, soluble starch and sodium lauryl sulfate; the protective agent is selected from turf, kaolin and sodium alginate or sodium carboxymethyl cellulose. The microbial powder is used for treating the landscaping waste and can meet the requirement for large-scale composting disposal of the landscaping waste.

Disclosed is a composite microbial agent for fermenting and degrading cellulose in straw. The composite microbial agent comprises bacillus subtilis, exiguobacterium and cellulomonas, a combination with high degradation efficiency is obtained through optimization and compounding, and the composite microbial agent can rapidly degrade the cellulose in the straw, which is beneficial to the resource utilization of the cellulose biomass waste.

The invention discloses a biological enzyme scouring and bleaching agent and a scouring and bleaching method for scouring and bleaching roving. The biological enzyme scouring and bleaching agent is a mixed solution obtained by the following steps: separately fermenting cellulomonas sp, trichodermareesei, bacillus cereus, pseudomonas and aspergillus niger (ASP-12) to obtain fermentation broth; centrifuging the fermentation broth to obtain respective biological enzyme solutions; mixing the respective biological enzyme solutions according to the volume ratio of 1:(1 to 10):(1 to 10):(1 to 10):(1 to 10). For flax roving scoured and bleached by adopting the method disclosed by the invention, the whiteness is 55 to 80 percent, the weight loss ratio is 7 to 15 percent, the residual gum content is 4 to 12 percent, the single-fiber tensile strength is 7 to 15cN/dtex, and the elongation at break is 6 to 12 percent. The scouring and bleaching method disclosed by the invention has the advantages of no pollution and zero discharge in the whole process and important theoretical significance and application value; the scouring and bleaching method accords with the development requirement of low-carbon economy and embodies a modern life concept of fashion bast fiber spinning and green bast fiber spinning.

The invention relates to a method for removing copper in wastewater by using sodium alginate immobilized cellulomonas. The method is characterized by comprising the following steps: culturing cellulomonas in a culture medium, immobilizing cellulomonas, and adding immobilized microspheres into to-be-purified polluted water containing copper ions. Compared with the prior art, the immobilized microsphere carrier prepared from sodium alginate has the advantages that a large number of carboxyl groups capable of being subjected to a complexation reaction with metal ions exist in molecules of the immobilized microsphere carrier, and the immobilized microsphere carrier is low in price and widely applied to free cells. The immobilized biotechnology has the advantages of being high in toxicity resistance, high in biological adsorption stability, high in pollutant removal speed and the like. The preparation steps are simple, the operation is convenient, the removal rate of low-concentration heavymetal copper in wastewater is high, the environmental protection benefit is obvious, and the application prospect is good.

The invention discloses a microbial agent for aerobic fermentation and rapid heating of organic solid waste in winter. The microbial agent is prepared from 50-70 parts of bacillus stearothermophilus, 50-70 parts of high-temperature-resistant pseudomonas, 50-70 parts of cellulomonas flavigena, 30-40 parts of bacillus subtilis, 30-40 parts of pseudomonas aeruginosa, 15-25 parts of ochrobactrum, 15-25 parts of clostridium, 15-25 parts of aspergillus niger, 5-15 parts of trichoderma viride, 5-15 parts of geotrichum candidum, 3-10 parts of white rot fungi, 3-10 parts of brown rot fungi, 1-5 parts of green algae and 1-5 parts of blue algae. A preparation method comprises the steps of (1), weighing of all the raw materials; (2), strain activation; (3), compounding of mixed strains; (4), preparation of a first-grade seed solution; and (5), preparation of the microbial agent. The microbial agent is novel in combination, high in viable count, high in synergistic effect and capable of achieving rapid heating and fermentation of organic solid waste piles.