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Method for removing copper in wastewater by using sodium alginate immobilized cellulomonas

A technology of cellulomonas and sodium alginate, applied in chemical instruments and methods, water treatment of special compounds, water pollutants, etc., can solve the problems of slow sewage purification speed, poor stability, and the possibility of secondary pollution, etc., to achieve Easy operation, high removal rate and good application prospect

Inactive Publication Date: 2020-04-03
NANHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional copper-containing wastewater treatment methods usually first use physical or chemical enrichment methods, and then concentrate removal methods, such as: distillation, chemical absorption and filtration, etc. After enrichment, the pollutants have poor stability and the possibility of secondary pollution is high. The overall sewage purification rate is slow

Method used

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  • Method for removing copper in wastewater by using sodium alginate immobilized cellulomonas
  • Method for removing copper in wastewater by using sodium alginate immobilized cellulomonas
  • Method for removing copper in wastewater by using sodium alginate immobilized cellulomonas

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method utilizing sodium alginate to immobilize cellulomonas to remove copper in wastewater, comprising the following steps:

[0026] Step 1. Cultivate Cellulomonas in culture medium, the medium components are tryptone: 10g / L, yeast extract: 5g / L, NaCl: 10g / L, adjust to pH by NaOH or HCI =7±0.1, the protective gas pure nitrogen and carbon dioxide, the volume ratio of the two is 4:1, passed through the bacterial filter into the sterilized medium for 15 minutes, under the action of the protective gas, the The bacteria in the logarithmic phase prepare the bacterial suspension, the bacteria in the logarithmic phase OD600=1.0±0.2, the concentration of the bacterial suspension is OD600=0.9±0.05, add the prepared bacterial suspension into the anaerobic sterile medium, and then Then put it into a constant temperature shaker, and cultivate it at a temperature of 30°C at a speed of 150r / min for 24 hours;

[0027] Step 2. Immobilization of Cellulomonas: Take 40 g of tryptone, 20...

Embodiment 2

[0038] In this example, the surface morphology and element changes of the immobilized microspheres were verified.

[0039] First, several immobilized microspheres were taken respectively, washed with ultrapure water for 3 times, and fixed in 2% glutaraldehyde solution for 6 hours. The processed samples were fixed in glass Petri dishes and freeze-dried in a desiccator for 24 h; then, the samples were covered with hexamethyldisilazane to improve the visibility of the samples under the microscope; finally, scanning electron microscopy (Zeiss EVO18) was used to analyze the morphology of bacteria before and after copper removal, and the energy spectrometer was used to analyze the element composition and the proportion of elements on the surface of immobilized microspheres before and after copper removal. see results figure 1 and figure 2, the surface of immobilized microspheres is relatively rough, and there are pores and channels, which provide attachment sites for copper ions ...

Embodiment 3

[0041] This example analyzes the role of immobilized microsphere functional groups in the copper removal process.

[0042] Fourier transform infrared spectrometer (Thermo Nicolet Avatar 460) was used to collect the changes of functional groups of immobilized microspheres before and after copper removal (4000-300 cm-1). Sample pretreatment method: Freeze-dry the sample, mix it with an appropriate amount of KBr crystals, and grind it completely until there are no small crystals, all of which are powdery, tightly attached to the wall of the mortar, and pressed into the mortar. see results image 3 , to analyze the changes in the peak positions of functional groups before and after copper removal. The peak positions before copper removal were 3434.61 cm-1 (amine group; -OH, N-H), 1720.82 cm-1 (protein amide band I; C=O), and 1460.97 cm-1 (Protein amide band II; N-H, C-H), 1026.63 cm-1 (phosphate radical and phosphate group), moved to 3442.82 cm-1, 1686.51 cm-1, 1460.70 cm-1, 953....

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Abstract

The invention relates to a method for removing copper in wastewater by using sodium alginate immobilized cellulomonas. The method is characterized by comprising the following steps: culturing cellulomonas in a culture medium, immobilizing cellulomonas, and adding immobilized microspheres into to-be-purified polluted water containing copper ions. Compared with the prior art, the immobilized microsphere carrier prepared from sodium alginate has the advantages that a large number of carboxyl groups capable of being subjected to a complexation reaction with metal ions exist in molecules of the immobilized microsphere carrier, and the immobilized microsphere carrier is low in price and widely applied to free cells. The immobilized biotechnology has the advantages of being high in toxicity resistance, high in biological adsorption stability, high in pollutant removal speed and the like. The preparation steps are simple, the operation is convenient, the removal rate of low-concentration heavymetal copper in wastewater is high, the environmental protection benefit is obvious, and the application prospect is good.

Description

technical field [0001] The invention relates to a sewage treatment method, in particular to a method for removing copper in waste water by using sodium alginate to immobilize Cellulomonas bacteria. Background technique [0002] Copper is an essential trace element for humans, animals and plants, but excessive copper will pose a threat to life. Copper-containing wastewater mainly comes from mining, electroplating, chemical industry, etc. Traditional copper-containing wastewater treatment methods usually first use physical or chemical enrichment methods, and then concentrate removal methods, such as: distillation, chemical absorption and filtration, etc. After enrichment, the pollutants have poor stability and the possibility of secondary pollution is high. The overall sewage purification rate is slow. Contents of the invention [0003] The purpose of the present invention is to solve the problems raised in the background technology, and to provide a kind of enrichment sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/20
CPCC02F3/34C02F2101/20C02F2305/06
Inventor 荣丽杉夏麟张诗琦袁彬胡俊彤赖晓波陈婉司
Owner NANHUA UNIV
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