Microbial agent for aerobic fermentation and rapid heating of organic solid waste in winter and preparation method thereof

A technology of aerobic fermentation and organic solid waste, applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effect of new bacterial agent combination, high number of viable bacteria, and accelerated biodegradation

Active Publication Date: 2021-12-10
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the object of the present invention is to provide a kind of bacteria agent and its preparation method ...

Method used

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  • Microbial agent for aerobic fermentation and rapid heating of organic solid waste in winter and preparation method thereof
  • Microbial agent for aerobic fermentation and rapid heating of organic solid waste in winter and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0040] Among the following examples 1-5:

[0041] 1. Bacillus stearothermophilus is Geobacillus stearothermophilus (Geobacillus stearothermophilus) YB1-1 strain, which was preserved on April 8, 2018 in the China Microbiological Culture Collection located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing The Center for General Microorganisms of the Management Committee, deposit number is CGMCC No.15559.

[0042] 2. The heat-resistant Pseudomonas sp. ZKB4-4 strain was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on July 12, 2012. Address: No. 1 Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, deposit number: CGMCC No.6351.

[0043] 3. Bacillus subtilis (Bacillus subtilis) Bs-03 was isolated from the soil of the experimental nursery of the Shandong Academy of Forestry Sciences by the soil dilution separation method, and was preserved in the G...

Embodiment 1

[0051] The bacterial agent used for rapid heating of organic solid waste in aerobic fermentation in winter includes the following raw materials: 60g of Bacillus stearothermophilus, 60g of Pseudomonas pyogenes, 60g of Cellulomonas flavinogenes, 35g of Bacillus subtilis, Pseudomonas aeruginosa 35g, Pseudomonas pallidum 20g, Clostridium 20g, Aspergillus niger 20g, Trichoderma viridans 10g, Geotrichum candidum 10g, White rot fungus 6g, Brown rot fungus 6g, Chlorella 3g and Cyanobacteria 3g, the total effective activity Bacterial count≥1×10 10 CFU / g.

[0052] The preparation method of the above-mentioned bacterial agent for rapid temperature rise in the aerobic fermentation of organic solid waste in winter specifically includes the following steps:

[0053] (1) Weigh each raw material

[0054] Take each bacterial strain by the weight of above-mentioned bacterial agent;

[0055] (2) Activation of bacteria

[0056] Inoculate each strain on a solid medium, culture at 52°C for 18 h...

Embodiment 2

[0066] Bacteria agent for rapid heating of organic solid waste aerobic fermentation in winter, including the following raw materials: 50g of Bacillus stearothermophilus, 50g of high-temperature-resistant Pseudoxanthomonas, 50g of Cellulomonas flavinogenes, 30g of Bacillus subtilis, Pseudomonas aeruginosa 30g, Pallidum 15g, Clostridium 15g, Aspergillus niger 15g, Trichoderma viridans 5g, Geotrichum candidum 5g, White rot fungus 3g, Brown rot fungus 3g, Chlorella 1g and Cyanobacteria 1g, total active activity Bacterial count≥1×10 10 CFU / g.

[0067] The preparation method of the above-mentioned bacterial agent for rapid temperature rise in the aerobic fermentation of organic solid waste in winter specifically includes the following steps:

[0068] (1) Weigh each raw material

[0069] Take each bacterial strain by the weight of above-mentioned bacterial agent;

[0070] (2) Activation of bacteria

[0071] Inoculate each strain on a solid medium, culture at 50°C for 16 hours, th...

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Abstract

The invention discloses a microbial agent for aerobic fermentation and rapid heating of organic solid waste in winter. The microbial agent is prepared from 50-70 parts of bacillus stearothermophilus, 50-70 parts of high-temperature-resistant pseudomonas, 50-70 parts of cellulomonas flavigena, 30-40 parts of bacillus subtilis, 30-40 parts of pseudomonas aeruginosa, 15-25 parts of ochrobactrum, 15-25 parts of clostridium, 15-25 parts of aspergillus niger, 5-15 parts of trichoderma viride, 5-15 parts of geotrichum candidum, 3-10 parts of white rot fungi, 3-10 parts of brown rot fungi, 1-5 parts of green algae and 1-5 parts of blue algae. A preparation method comprises the steps of (1), weighing of all the raw materials; (2), strain activation; (3), compounding of mixed strains; (4), preparation of a first-grade seed solution; and (5), preparation of the microbial agent. The microbial agent is novel in combination, high in viable count, high in synergistic effect and capable of achieving rapid heating and fermentation of organic solid waste piles.

Description

technical field [0001] The invention relates to the technical field of bacterial agents, and more specifically relates to a bacterial agent for rapid heating of organic solid waste in aerobic fermentation in winter and a preparation method thereof. Background technique [0002] Organic solid waste refers to solid organic objects and substances that have lost their original use value or have not lost their use value but have been discarded or abandoned during production activities, including agricultural organic waste (mainly including crop straw vines, livestock and poultry waste, etc.) Feces and aquatic waste, etc.), industrial organic waste (mainly including organic waste residues, etc.), municipal organic waste (mainly including landscaping waste, municipal sludge, animal inclusions from slaughterhouses, and kitchen waste, etc.). With the rapid development of my country's economy and the continuous improvement of people's living standards, organic solid waste presents the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/12C05F17/20C05F17/80C12R1/07C12R1/64C12R1/125C12R1/385C12R1/01C12R1/145C12R1/685C12R1/885C12R1/89C12R1/645
CPCC12N1/20C12N1/14C12N1/12C05F17/20C05F17/80Y02W30/40
Inventor 马闯金凯郑国砥张肖静魏明宝赵继红张宏忠
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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