Household sludge reduction compound microbial preparation as well as preparation and application methods thereof

A technology of composite microorganisms and domestic sludge, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that the composite microbial agent does not conform to physical sludge, does not conform to the concept of clean production, and has large funds. To achieve the effect that is conducive to rapid proliferation

Active Publication Date: 2014-10-08
ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are two problems of effectiveness and economy in the treatment and disposal of excess sludge: first, there is no effective method that can be generalized and has no pollution to the environment; second, various sludge treatment and Dis

Method used

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  • Household sludge reduction compound microbial preparation as well as preparation and application methods thereof
  • Household sludge reduction compound microbial preparation as well as preparation and application methods thereof
  • Household sludge reduction compound microbial preparation as well as preparation and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Step 1: Carry out amplified culture separately

[0051] Cellulomonas CCTCC AB209393 was inoculated in Erlenmeyer flasks containing LB liquid medium after being activated on a slant, and cultured at 30°C for 36 hours. Put the above cultured bacterial solution into the seed tank according to the inoculum amount of 5%, the air volume of the seed tank is 1:0.5, the culture temperature is 30°C, the stirring speed is 120 rpm, the culture time is 48 hours, and cultivated to the logarithmic growth phase;

[0052] After Serratia CICC20143 was activated on a slant, it was inoculated in a Erlenmeyer flask containing LB liquid medium and cultured at 30°C for 48 hours. Put the above cultured bacterial solution into the seed tank according to the inoculum amount of 5%, the air volume of the seed tank is 1:1.5, the culture temperature is 30°C, the stirring speed is 150rpm, and the culture time is 60h;

[0053] After the Bacillus pumilus ACCC1011 was activated on the slant, it was ino...

Embodiment 2

[0059] Step 1: Carry out amplified culture separately

[0060] Cellulomonas CCTCC AB209393 was inoculated in Erlenmeyer flasks containing LB liquid medium after being activated on a slant, and cultured at 37°C for 48 hours. Put the above cultured bacterial solution into the seed tank according to 5% inoculation amount, the air volume of the seed tank is 1:0.5, the culture temperature is 30°C, the stirring speed is 150rpm, the culture time is 72h, and the culture is until the logarithmic growth phase;

[0061] Serratia CICC20143 was inoculated in a Erlenmeyer flask containing LB liquid medium after being activated on a slant, and cultured at 37°C for 48 hours. Put the above cultured bacterial solution into the seed tank according to 10% inoculation amount, the air volume of the seed tank is 1:1.5, the culture temperature is 30°C, the stirring speed is 160rpm, the culture time is 60h, and cultivate to the logarithmic growth phase;

[0062]After the Bacillus pumilus ACCC1011 was...

Embodiment 3

[0068] Step 1: Carry out amplified culture separately

[0069] After being activated on a slant, inoculate in an Erlenmeyer flask containing LB liquid medium, and culture at 30°C for 48 hours. Put the above cultured bacterial solution into the seed tank according to 10% inoculation amount, the air volume of the seed tank is 1:1.5, the culture temperature is 30°C, the stirring speed is 160rpm, the culture time is 60h, and cultivate to the logarithmic growth phase;

[0070] Rhodotorula colloides CICC31192 was inoculated in a Erlenmeyer flask containing YPD liquid medium after being activated on a slant, and cultured at 28°C for 56 hours. Put the above cultured bacterial solution into the seed tank according to the inoculum amount of 5%, the air volume of the seed tank is 1:1.5, the culture temperature is 30°C, the stirring speed is 120rpm, the culture time is 48h, and cultivate to the logarithmic growth phase;

[0071] Viable bacteria count, the number of detected bacteria, mix...

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Abstract

The invention relates to a household sludge reduction compound microbial preparation as well as preparation and application methods thereof, belonging to the field of microbial application. Based on the total bacteria count, the household sludge reduction compound microbial preparation comprises 10-20% of Celloulonmonassp., 10-20% of Serratiasp., 30-60% of Bacilluspumilus and 20-30% of Rhodotorulamucilaginosa, wherein the Celloulonmonassp. is selected from at least one of CCTCCAB209393 and YMCCKMS3990, the Serratiasp. is selected from at least one of ACCC02490 and CICC20143, the Bacilluspumilus is ACCC10113 and the Rhodotorulamucilaginosa is CICC31192. The microbial preparation and the preparation method provided by the invention are simple and feasible, economic and reasonable and free from secondary pollution to the environment.

Description

technical field [0001] The invention relates to a domestic sludge weight reduction composite microbial preparation and a preparation and application method thereof, which belong to the field of microbial application. Background technique [0002] With the expansion of city size and population, the amount of sewage treatment is increasing year by year. As of the end of June 2013, a total of 3,479 urban sewage treatment plants have been built in cities and counties across the country, and 650 cities have built sewage treatment plants nationwide, accounting for about 98.9% of the total number of cities with cities, forming a sewage treatment capacity of 1.2 ×10 8 m 3 / d. [0003] At present, the activated sludge method is a common method of sewage treatment. While the effluent reaches the standard, it is accompanied by a large amount of excess sludge with high water content. The moisture content of the remaining sludge is generally above 95%, and the content of organic matt...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F11/02C12R1/425C12R1/07C12R1/645C12R1/01
Inventor 赵海泉张龙胡子全汪顺丽宋永庆张宏才柴华建魏荷芬
Owner ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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