Straw degradation acidification microbial inoculum and manufacturing method thereof

A technology for straw and bacterial agent, which is applied in the field of straw degrading and acidifying bacterial agent and its manufacture, can solve the problems of harsh cultivation conditions, unclear composition of the target straw for degradation, long time, etc. and the effect of high xylanase activity and broad application prospects

Active Publication Date: 2016-11-23
中农绿能科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological pretreatment technology, due to its advantages of low cost, low energy consumption, environmental friendliness, and no need to use chemicals, has attracted the attention of researchers and engineers in recent years, but there are still some problems as follows: during the pretreatment process, degradation The object (stalk composition) is not clear
In the pretreatment of fungi, the biggest disadvantage is that the time required for pretreatment is longer (more than 3 weeks), and it must be propagated under aerobic conditions, and the cultivation conditions are relatively harsh.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of stalk degradation acidifying bacterial agent

[0044] (1) Activation of bacteria

[0045] Cellulomonas flavigena (Cellulomonas flavigena) ACCC No.11055 was inoculated in the modified medium of peptone cellulose culture solution (PCS), cultured statically at room temperature (30°C) for 1 day, and all the fermentation broth was collected;

[0046] Inoculate Clostridium cellulolyticum (Clostridium cellulolyticum) ACCC No.00522 in the modified medium of peptone cellulose culture solution (PCS), culture it statically at room temperature (30°C) for 1 day, and collect all the fermentation broth;

[0047] Bacillus subtilis (Bacillus subtilis) ACCC No.11089 was inoculated in peptone cellulose culture medium (PCS) modified medium, cultured statically at room temperature (30°C) for 1 day, and all the fermentation broth was collected;

[0048] Bacillus cereus (Bacillus cereus) ACCC No.01955 was inoculated in peptone cellulose culture medium (PCS) m...

Embodiment 2

[0062] Example 2, Straw Degradation Acidification Bacteria Applied to Acidification Pretreatment of Corn Stalk Anaerobic Fermentation

[0063] Experimental location: Shangzhuang Experimental Station of China Agricultural University, Haidian District, Beijing;

[0064] Experimental materials: corn stalks, the straw-degrading acidifying bacterial agent prepared in Example 1;

[0065] Experimental design: set up two acidification tanks, each with a volume of 1 cubic meter, and run each acidification tank continuously for ten hydraulic retention times, while ensuring that other experimental conditions are the same.

[0066] Treatment 1: without adding the straw-degrading acidifying bacterial agent prepared in Example 1.

[0067] Treatment 2: use the straw-degrading acidifying bacterial agent prepared in Example 1.

Embodiment approach

[0068] Implementation method: corn stalks are pulverized into 1-2 cm, and 30 kilograms of stalk-degrading acidification bacterial agents prepared in Example 1 are inoculated by every cubic meter of acidification tank volume (bacteria agent addition is 3% of the acidification tank volume), and the remaining volume is made of tap water Supplement, the hydraulic retention time is 3 days, and it runs continuously for 30 days. Feed 60Kg of corn stalks (by dry weight) every day.

[0069] The content of cellulose, hemicellulose and volatile organic acid was detected every day, and the degradation rate was calculated. The detection method of cellulose, hemicellulose and volatile organic acids is based on the literature: "Determination of cellulose and hemicellulose content in corn straw by near-infrared spectroscopy", Liu Huiying, Li Guoli, Xue Donghua, Xu Hongzhang, Ye Xiaoquan, China Agricultural Science Bulletin, 2013, 29(35): 182-186, cellulose degradation rate = decomposed cellu...

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Abstract

The invention discloses a straw degradation acidification microbial inoculum and a manufacturing method thereof. Active ingredients of the composite microbial inoculum for degrading straw are cellulomonas flavigena, clostridium cellulolyticum, bacillus subtilis and bacillus cereus. The microbial inoculum breaks through the limitation that fungi and lytic enzymes thereof cannot quickly and efficiently decompose natural cellulose, a key pretreatment technology is provided for straw biogas fermentation, and the straw degradation acidification microbial inoculum has wide application prospect in the field of preparation of biogas from lignocellulose raw materials such as the straw.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a straw-degrading acidifying bacterial agent and a manufacturing method thereof. Background technique [0002] With the rapid development of my country's economy, the requirements for environmental protection and energy requirements are getting higher and higher. Further preparation of biogas from waste through biogas fermentation can not only solve the pollution problem of waste, but also deal with the shortage of fossil energy to a certain extent. Traditional biogas fermentation raw materials such as livestock and poultry manure, organic waste water, etc., can no longer meet the industrial development of biogas. At present, about 700 million tons of straw are produced in my country every year, and its gas production potential is huge. Lignocellulose, the main component of straw, is composed of cellulose, hemicellulose, and lignin tightly combined through complex chemical bonds. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P5/02C12R1/01C12R1/145C12R1/085C12R1/125
Inventor 袁旭峰崔宗均朱万斌王小芬姚建明师力民
Owner 中农绿能科技集团有限公司
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