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Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof

A compound fermentation inoculum and a preparation method technology are applied in the field of microbial compound fermentation inoculum and its preparation, which can solve the problems affecting the large-scale promotion of fermentation bed breeding technology, the insignificant effect of livestock and poultry waste treatment, and high capital requirements and technical requirements. problems, to achieve the effect of improving animal welfare, reducing breeding costs, and improving the breeding environment

Active Publication Date: 2014-06-04
河北沃稼农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to polluting soil and water bodies, livestock and poultry waste will also produce a large amount of odorous gases such as ammonia, hydrogen sulfide, mercaptan and methyl mercaptan, which will adversely affect the environment and the normal life of humans and animals.
[0003] At present, domestic large and medium-sized farms generally adopt the model of standard discharge, planting and breeding balance, and biogas ecology to deal with livestock and poultry waste, but these methods require high capital and technical requirements; many small pig farms continue to use traditional waste directly as agricultural fertilizer method, low waste utilization rate and easy to pollute the environment
In recent years, fermented bed cultivation introduced from Japan has attracted widespread attention due to its easy operation, low capital investment, and good treatment effect. The degradation efficiency of livestock and poultry waste is low, and its treatment effect on livestock and poultry waste is not very significant, which affects the promotion of fermentation bed breeding technology on a large scale. Therefore, an efficient and targeted fermentation bed compound bacterial agent is urgently needed to solve the problem. The above technical problems

Method used

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  • Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof
  • Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof

Examples

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Embodiment 1

[0028] The present invention is a composite fermented bacterial agent for treating livestock and poultry waste, the active component of which is bacillus ( Bacillus beringensis ) The preservation number is CGMCC No.6611, Rhodobacter thiaphila ( Rhodobacter sulfidophilus ) preservation number is CGMCC No.6613 and Cellulomonas ( Cellulomonas sp. .) The preservation number is CGMCC No. 6612, and the strains of Bacillus, Thiophilus and Cellulomonas are mixed according to the mass ratio of 0.5-1.5:0.5-1.5:0.5-1.5. The specific preparation method is as follows:

[0029] (1) Incline culture: Bacillus ( Bacillus beringensis) The preservation number is CGMCC No.6611, Rhodobacter thiaphila ( Rhodobacter sulfidophilus ) preservation number is CGMCC No.6613 and Cellulomonas ( Cellulomonas sp. .) The preservation number is CGMCC No. 6612, respectively inoculated on the corresponding slant culture medium, at pH 5.8-7.2, temperature 28-37 ℃, static culture for 33-96 hours, and stored...

Embodiment 2

[0040] The present invention is a composite fermented bacterial agent for treating livestock and poultry waste, the active component of which is bacillus ( Bacillus beringensis ) The preservation number is CGMCC No.6611, Rhodobacter thiaphila ( Rhodobacter sulfidophilus ) preservation number is CGMCC No.6613 and Cellulomonas ( Cellulomonas sp. .) The preservation number is CGMCC No. 6612, and the strains of Bacillus, Rhodobacter thiarophilus and Cellulomonas are mixed in a mass ratio of 1:1:1, and the preparation method is the same as that of Example 1 above.

Embodiment 3

[0042] The present invention is a composite fermented bacterial agent for treating livestock and poultry waste, the active component of which is bacillus ( Bacillus beringensis ) The preservation number is CGMCC No.6611, Rhodobacter thiaphila ( Rhodobacter sulfidophilus ) preservation number is CGMCC No.6613 and Cellulomonas ( Cellulomonas sp. .) The deposit number is CGMCC No. 6612. The strains of Bacillus, Rhodobacter thiaphila and Cellulomonas were mixed in a mass ratio of 0.5:1:0.5, and the preparation method was the same as that in Example 1 above.

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Abstract

The invention discloses a composite fermentation inoculant for processing animal wastes, and a preparation method thereof. The composite fermentation inoculant is characterized in that the active components of the composite fermentation inoculant comprise Bacillus beringensis, Rhodobacter sulfidophilus and Cellulomona ssp.; the mass ratio of the Bacillus beringensis to the Rhodobacter sulfidophilus to the Cellulomona ssp. is 0.5-1.5:0.5-1.5:0.5-1.5; and the preparation method comprises the following steps: carrying out inclined strain culture of the Bacillus beringensis, the Rhodobacter sulfidophilus and the Cellulomona ssp.; carrying out liquid seed enlargement culture to obtain a Bacillus beringensis broth, a Rhodobacter sulfidophilus broth and a Cellulomona ssp. broth; and mixing the above broths according to a mass ratio of 0.5-1.5:0.5-1.5:0.5-1.5, inoculating the mixed broth to a solid enlargement culture medium with the inoculation amount of 4-5%(w / v), and carrying out enlargement culture to obtain the composite fermentation inoculant. The composite fermentation inoculant has the advantages of deodorizing, inhibition of the growth of pathogenic microorganisms in the animal wastes, improvement of the content of proteins in the animal wastes, high processing efficiency and strong pertinence.

Description

technical field [0001] The invention relates to a composite fermentation bacterial agent and a preparation method thereof, in particular to a microbial composite fermentation bacterial agent for treating livestock and poultry waste, a preparation method and an application thereof. Background technique [0002] Livestock and poultry waste refers to a large amount of aquaculture waste produced in the process of livestock and poultry breeding, which has become one of the three major sources of environmental pollution due to its growing discharge. Livestock and poultry waste mainly includes livestock and poultry manure, urine, feed residues and sewage from washing pens. In addition to polluting soil and water bodies, livestock and poultry waste will also produce a large amount of odorous gases such as ammonia, hydrogen sulfide, mercaptan and methyl mercaptan, which will adversely affect the environment and the normal life of humans and animals. [0003] At present, domestic lar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20A01K1/015C12R1/01C12R1/07
Inventor 吴祖芳翁佩芳毕泗伟钟仁方
Owner 河北沃稼农业科技有限公司
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