A kind of microbial preparation for repairing hydroxymethylcellulose wastewater and its preparation method

Abstract

The invention belongs to the technical field of microorganisms and discloses a microbial preparation for repairing hydroxymethyl cellulose wastewater. The microbial preparation is prepared from the following raw materials: anabaena variabilis domestication liquid, cellulomonas flavigena domestication liquid, bacillus subtilis domestication liquid and Aspergillus niger domestication liquid, wherein the anabaena variabilis domestication liquid, the cellulomonas flavigena domestication liquid, the bacillus subtilis domestication liquid and the Aspergillus niger domestication liquid are separately used. According to the microbial preparation disclosed by the invention, four microorganisms are adopted, reasonably matched and well synergized, so that proliferation and enzyme activity of the microorganisms can be promoted; the microbial preparation has a good effect for repairing hydroxymethyl cellulose wastewater.

Description

technical field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial preparation for repairing hydroxymethylcellulose wastewater and a preparation method thereof. Background technique

[0002] Carboxymethyl cellulose (CMC) is a cellulose derivative, which belongs to anionic cellulose ethers. Its appearance is white or slightly yellow flocculent fiber powder or white powder, odorless, tasteless, non-toxic; easily soluble in cold water or Hot water forms a transparent solution with a certain viscosity. The solution is neutral or slightly alkaline, insoluble in ethanol, ether, isopropanol, acetone and other organic solvents, but soluble in 60% ethanol or acetone solution. It is hygroscopic and stable to light and heat. The viscosity decreases with the increase of temperature. The solution is stable when the pH value is 2 to 10. When the pH value is lower than 2, there is solid precipitation. When the pH value is higher...

Examples

Embodiment 1

[0029] A microbial preparation for repairing hydroxymethylcellulose wastewater, which is composed of the following raw materials: acclimatization liquid of Anabaena variabilis, acclimatization liquid of Cellulomonas chrysogenum, acclimation liquid of Bacillus subtilis, acclimation liquid of Aspergillus niger; The above-mentioned Anabaena variabilis liquid, Cellulomonas flavinogenes domestication liquid, Bacillus subtilis domestication liquid and Aspergillus niger domestication liquid are used separately.

[0030] The Anabaena variabilis domestication solution is prepared according to the following process: take hydroxymethylcellulose wastewater, add 0.5g of urea, 6g of sodium bicarbonate, 0.2g of dipotassium hydrogenphosphate, and 0.2g of potassium dihydrogenphosphate to the wastewater , sodium chloride 0.3g, potassium chloride 0.3g, ferric ammonium citrate 0.03g, edetate disodium 0.03g, stir evenly, and set the volume to 1L to prepare algae acclimation medium; The algae were ...

Embodiment 2

[0038] A microbial preparation for repairing hydroxymethylcellulose wastewater, which is composed of the following raw materials: acclimatization liquid of Anabaena variabilis, acclimatization liquid of Cellulomonas chrysogenum, acclimation liquid of Bacillus subtilis, acclimation liquid of Aspergillus niger; The above-mentioned Anabaena variabilis liquid, Cellulomonas flavinogenes domestication liquid, Bacillus subtilis domestication liquid and Aspergillus niger domestication liquid are used separately.

[0039]The Anabaena variabilis domestication solution is prepared according to the following process: take hydroxymethylcellulose wastewater, add 0.5g of urea, 6g of sodium bicarbonate, 0.2g of dipotassium hydrogenphosphate, and 0.2g of potassium dihydrogenphosphate to the wastewater , sodium chloride 0.3g, potassium chloride 0.3g, ferric ammonium citrate 0.03g, edetate disodium 0.03g, stir evenly, and set the volume to 1L to prepare algae acclimation medium; The algae were c...

Embodiment 3

[0047] The hydroxymethyl cellulose wastewater used in Example 1-2 of the present invention is pretreated wastewater, and the pretreatment steps are: the hydroxymethyl cellulose production wastewater first passes through the flocculation sedimentation tank, the flocculant is aluminum sulfate, and the addition amount is 1-3wt%, and then treated by micro-electrolysis, and then enter the acid hydrolysis adjustment tank, adjust the pH of the solution to 6.5-7; the main indicators of the pretreated wastewater are: COD 1094 mg / L, BOD 613 mg / L, ammonia nitrogen 215 mg / L, SS 41 mg / L, total salt 389 mg / L, cellulose 326 mg / L, hydroxymethyl cellulose 592 mg / L.

[0048] Taking Example 1 of the present invention as an example, the use method of the microbial preparation of the present invention is: enter the microbial reaction tank through the pretreated waste water, inoculate the Anabaena variabilis domestication solution according to the inoculum size of 4%, after cultivating for 12 hours...

technical field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial preparation for repairing hydroxymethylcellulose wastewater and a preparation method thereof. Background technique

[0002] Carboxymethyl cellulose (CMC) is a cellulose derivative, which belongs to anionic cellulose ethers. Its appearance is white or slightly yellow flocculent fiber powder or white powder, odorless, tasteless, non-toxic; easily soluble in cold water or Hot water forms a transparent solution with a certain viscosity. The solution is neutral or slightly alkaline, insoluble in ethanol, ether, isopropanol, acetone and other organic solvents, but soluble in 60% ethanol or acetone solution. It is hygroscopic and stable to light and heat. The viscosity decreases with the increase of temperature. The solution is stable when the pH value is 2 to 10. When the pH value is lower than 2, there is solid precipitation. When the pH value is higher...

Examples

Embodiment 1

[0029] A microbial preparation for repairing hydroxymethylcellulose wastewater, which is composed of the following raw materials: acclimatization liquid of Anabaena variabilis, acclimatization liquid of Cellulomonas chrysogenum, acclimation liquid of Bacillus subtilis, acclimation liquid of Aspergillus niger; The above-mentioned Anabaena variabilis liquid, Cellulomonas flavinogenes domestication liquid, Bacillus subtilis domestication liquid and Aspergillus niger domestication liquid are used separately.

[0030] The Anabaena variabilis domestication solution is prepared according to the following process: take hydroxymethylcellulose wastewater, add 0.5g of urea, 6g of sodium bicarbonate, 0.2g of dipotassium hydrogenphosphate, and 0.2g of potassium dihydrogenphosphate to the wastewater , sodium chloride 0.3g, potassium chloride 0.3g, ferric ammonium citrate 0.03g, edetate disodium 0.03g, stir evenly, and set the volume to 1L to prepare algae acclimation medium; The algae were ...

Embodiment 2

[0038] A microbial preparation for repairing hydroxymethylcellulose wastewater, which is composed of the following raw materials: acclimatization liquid of Anabaena variabilis, acclimatization liquid of Cellulomonas chrysogenum, acclimation liquid of Bacillus subtilis, acclimation liquid of Aspergillus niger; The above-mentioned Anabaena variabilis liquid, Cellulomonas flavinogenes domestication liquid, Bacillus subtilis domestication liquid and Aspergillus niger domestication liquid are used separately.

[0039]The Anabaena variabilis domestication solution is prepared according to the following process: take hydroxymethylcellulose wastewater, add 0.5g of urea, 6g of sodium bicarbonate, 0.2g of dipotassium hydrogenphosphate, and 0.2g of potassium dihydrogenphosphate to the wastewater , sodium chloride 0.3g, potassium chloride 0.3g, ferric ammonium citrate 0.03g, edetate disodium 0.03g, stir evenly, and set the volume to 1L to prepare algae acclimation medium; The algae were c...

Embodiment 3

[0047] The hydroxymethyl cellulose wastewater used in Example 1-2 of the present invention is pretreated wastewater, and the pretreatment steps are: the hydroxymethyl cellulose production wastewater first passes through the flocculation sedimentation tank, the flocculant is aluminum sulfate, and the addition amount is 1-3wt%, and then treated by micro-electrolysis, and then enter the acid hydrolysis adjustment tank, adjust the pH of the solution to 6.5-7; the main indicators of the pretreated wastewater are: COD 1094 mg / L, BOD 613 mg / L, ammonia nitrogen 215 mg / L, SS 41 mg / L, total salt 389 mg / L, cellulose 326 mg / L, hydroxymethyl cellulose 592 mg / L.

[0048] Taking Example 1 of the present invention as an example, the use method of the microbial preparation of the present invention is: enter the microbial reaction tank through the pretreated waste water, inoculate the Anabaena variabilis domestication solution according to the inoculum size of 4%, after cultivating for 12 hours...

Images