Composite microbial agent for fermenting and degrading cellulose in straw

A composite microbial inoculum and cellulose technology, applied in the directions of microorganism-based methods, microorganisms, fermentation, etc., can solve the problems of poor treatment effect, reduced enzyme activity, limited degree of cellulose degradation, etc., to achieve the best treatment effect, speed up The effect of cellulose degradation and optimal bioavailability

Active Publication Date: 2021-06-29
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme secreted by a single strain is relatively small, the enzyme activity is re

Method used

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  • Composite microbial agent for fermenting and degrading cellulose in straw
  • Composite microbial agent for fermenting and degrading cellulose in straw
  • Composite microbial agent for fermenting and degrading cellulose in straw

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Cellulase Activity Determination and Filter Paper Disintegration Experiment

[0037] 1. Experimental materials

[0038] (1) Compound microbial agent

[0039] Bacillus subtilis, Microbacterium, and Cellulomonas were respectively cultured in beef extract peptone medium to logarithmic growth phase, Aspergillus and Rhizopus oryzae were respectively cultured in PDA medium to logarithmic growth phase, and the The culture solution is formulated into a compound microbial bacterial agent according to the following proportions.

[0040] Experimental group-1, the volume ratio of Bacillus subtilis, Aspergillus, Microbacterium, Cellulomonas, Rhizopus oryzae is 2:2:1:1:1;

[0041] In experimental group-2, the volume ratio of Bacillus subtilis, Microbacterium and Cellulomonas was 2:1:1.

[0042] (2) Filter paper degradation experiment

[0043] The above two groups of composite microbial agents were added to the medium with filter paper as the only carbon source, and the ...

Embodiment 2

[0065] (1) Fermentation and degradation of corn stalks by compound microbial agents

[0066] Take the pretreated corn stalk 25g and put it into the Erlenmeyer flask, adjust the initial water content to be 70%, add the composite microbial inoculant (experimental group-2) described in Example 1 of the total water 2%, the logarithm of it comprises The volume ratio of the culture solution of Bacillus subtilis, Microbacterium and Cellulomonas in the growth phase is 2:1:1 respectively, and the treatment is 20 days. The experiment set up the blank group without adding bacterial agents, the control group-1 (only added 2% of Bacillus subtilis), the control group-2 (only added 2% of microbacteria) and the control group-3 (only added 2% of fiber Monas), each treatment was done in 3 parallels.

[0067] (2) Determination of cellulose and hemicellulose content by differential gravimetric method

[0068] Weigh 1.5 g of straw, and bake at 60°C to constant weight W1. Add 150mL of 2mol / L HCl...

Embodiment 3

[0072] (1) Fermentation and degradation of corn stalks by compound microbial agents

[0073] Take the pretreated corn stalk 25g and put it into the Erlenmeyer flask, adjust the initial water content to be 60%, add 2% composite microbial bacterial agent (experimental group-3) and 2% composite microbial bacterial agent (experimental group-4) respectively ), the composition of the experimental group-3 is that the volume ratio of the logarithmic growth phase Bacillus subtilis, microbacteria, and cellulomonas culture solution is 1:1:1 respectively, and the composition of the experimental group-4 is the logarithmic growth phase Bacillus subtilis The volume ratio of Bacillus, Microbacterium, Cellulomonas and Streptomyces culture solution is 2:2:2:1 respectively, and the treatment is 30 days. In the experiment, a blank group without adding bacterial agents was set up, and 3 parallels were done for each treatment.

[0074] (2) Determination of cellulose and hemicellulose content by di...

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Abstract

Disclosed is a composite microbial agent for fermenting and degrading cellulose in straw. The composite microbial agent comprises bacillus subtilis, exiguobacterium and cellulomonas, a combination with high degradation efficiency is obtained through optimization and compounding, and the composite microbial agent can rapidly degrade the cellulose in the straw, which is beneficial to the resource utilization of the cellulose biomass waste.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of cellulosic biomass waste, in particular to a compound microbial bacterial agent for fermenting and degrading cellulose in straw and a degradation method. Background technique [0002] With the continuous improvement of my country's agricultural production scale, the output of agricultural solid waste is increasing year by year. The annual output of corn, rice and wheat straw is about 1.1 billion tons. If this kind of waste is not utilized, it will cause serious air pollution and soil and water pollution. Pollution not only wastes resources, but also seriously affects the ecological environment and human health. [0003] Cellulosic waste is a kind of renewable energy with great potential for resource utilization. It is green, clean, collectable-storage-transportable, and can be converted into energy products and chemicals. Therefore, the resource utilization of cellulosic biomass ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P19/02C12R1/125C12R1/01C12R1/845C12R1/465C12R1/69
CPCC12N1/20C12N1/14C12P39/00C12P19/02
Inventor 武双程艳玲华威王晚晴
Owner BEIJING UNION UNIVERSITY
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