5621 results about "Subtilis bacillus" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention discloses a hametz used for fermenting tobacco and applications thereof. The hametz is characterized by comprising three strains of Bacillus subtilis, lactobacillus and microzyme, the viable count ratio of which is 10 to 50: 2 to 30: 2 to 30. The hametz can ferment waste material of the tobacco or the flavor used for producing natural cigarettes by using the tobacco and can be used for tobacco fermentation. The flavor used for the natural cigarettes prepared by the hametz has a velvet fragrance and can remarkably increase the fragrance, reduce the unpurified gas and reduce the thrill as well when the flavor is used in the cigarettes, thus leading the smoke gas to be lingering and smooth. The hametz used for fermenting tobacco leaves can remarkably shorten the alcoholizing time and improve the quality of the tobacco leaves.

The invention relates to a composite microbial fertilizer for controlling crop diseases and insect pests, which contains composite microbial fungicide and is prepared by the following raw materials based on parts by weight: 10-35 parts of composite microbial fertilizer fungicide, 3-15 parts of composite microbial pesticide fungicide, 1-5 parts of insecticidal synergist, 1-5 parts of plant growth regulator, 20-60 parts of grass carbon, 5-15 parts of expanded chicken manure, 5-50 parts of inorganic fertilizer and 1-3 parts of binding agent, wherein the composite microbial fertilizer fungicide is prepared by bacillus megaterium and jelly-like bacillus; and the composite microbial pesticide fungicide is prepared by mixing bacillus thuringiensis and bacillus subtilis, adding sodium percarbonate type solid state oxygen-increasing agent for mixing and fermenting and finally absorbing by the grass carbon. The composite microbial fertilizer adopts strains with good suitability, and the strains are compounded according to a certain proportion to obtain the composite fungicide which has durable activity and stable performance; and the four beneficial strains are compatible with each other and performs the own functions, so that the multiple aims of resisting germs as well as diseases and insect pests, and increasing the crop yield can be achieved.

A method for treatment or prophylaxis of a disease state or other physiological condition, e.g., autism, delayed development, acid reflux disease, vaginal yeast infections, impaired hearing, chronic ear infections, seasonal allergies, Fibromyalgia syndrome, Crohn's disease, colitis, irritable bowel syndrome, interstitial cystitis, acne, sinusitis, rheumatoid arthritis, chronic fatigue syndrome, asthma, attention deficit disorder, attention deficit/hyperactivity disorder, rosacea, multiple sclerosis, hyperglycemia, or Ménière's disease, by administration of an anti-fungal composition that includes at least one of the bacilli (1) Bacillus subtilis, (2) Lactobacillus sporogenes, and (3) Streptococcus faecalis.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

Antimicrobial compounds from Bacillus subtilis for use against animal and human pathogens. A novel strain of Bacillus subtilis was isolated from the gastrointestinal tract of poultry and was found to produce a factor or factors that have excellent inhibitory effects on Clostridium perfringens, Clostridium difficile, Campylobacter jejuni, Campylobacter coli, and Streptococcus pneumoniae. The factor(s) retain full viability and antimicrobial activity after heat treatment. The invention provides a method of treatment of pathogenic microorganisms including C. perfringens.

The invention relates to anti-bacterial water-based paint and a preparation method thereof. The paint comprises the following components in parts by weight: 0.2-11 parts of anti-bacterial agent, 8-33 parts of nano material, 23-64 parts of water-based resin dispersoid and 0.75-18 parts of adhesive resin or plasticizer. The preparation method comprises the following steps: firstly preparing a nano silver anti-bacterial agent; mixing deionized water, the anti-bacterial agent, a wetting agent, a dispersing agent and a defoaming agent and uniformly mixing, adding the nano material, uniformly dispersing to obtain the water-based dispersoid; adding the obtained water-based dispersoid to the mixed emulsion or water-based resin dispersoid, then adding the adhesive resin or plasticizer and various conventional assistants, stirring and dispersing evenly; adding pigments or colorant; and supplementing water to obtain the anti-bacterial water-based paint. The long-acting broad-spectrum antibacterial water-based paint has high fungicidal efficiency (more than 99%) on escherichia coli, staphylococcus aureus, black varietas of bacillus subtilis and the like and can reduce the high concentrate of organic matters of formaldehyde to the range of specified concentration index.

The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g/T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g/T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg/g, the reducing sugar reaches 20-80mg/g, the GABA reaches 0.317-0.5mg/g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu/g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU/g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

This invention discloses a solid microbial agent for degrading petroleum pollutants and petroleum products. The solid microbial agent is prepared by: mixing primary seed solutions of Bacillus subtilis and Sphingobacterium multivorum at a volume ratio of (0.5-20):1, fermenting to obtain mixed bacterial fermentation solution, and mixing the mixed bacterial fermentation solution and turfy soil at a weight ratio of (0.1-1):1. The active living bacteria number of Bacillus subtilis is 106-1010/mL, and that of Sphingobacterium multivorum is 107-1012/mL. The solid microbial agent has such advantages as high activity in soil, rapid growth and no secondary pollution to environment, and can be used for biological bioremediation of petroleum polluted soil and petroleum product leakage fields.

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g/T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W/W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g/T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

The invention relates to bsacillus subtilis and microbial inoculum using the bsacillus subtilis as active ingredients, which are applied to the field of fruit freshness keeping. The bsacillus subtilis related by the invention is named as Bsacillus subtilis J18, which is preserved in China General Microbiological Culture Preservation and Management Center at present with the preservation number of CGMCC NO: 3665. Strains are prepared into fermentation agents after fermentation culture, and the fermentation agents are respectively mixed with calcium chloride and chitosan solution to be prepared into dry powder preparations and fruit glaze agents with obvious prevention and treatment effects on pear melasma after picking, black rot of pear, grape grey mould, watermelon wilt and the like. The bsacillus subtilis J18 belongs to a strain of antagonistic bacteria with wide-spectrum resistance on fruit and crop diseases.

Belonging to the technical field of bioengineering, the invention discloses a bacillus subtilis for efficient exogenous protein expression and high density culture. According to the invention, a screening and separation method is employed to acquire a bacillus subtilis with high protein expression quantity, and the six genes srfC, spoIIAC, amyE, nprE, aprE and bamA are knocked out to obtain B. subtilis WS5 with a preservation number of CCTCC M 2016536. The bacillus subtilis WS5 is adopted as the expression host for construction of a beta-CGTase genetically engineered bacterium. The beta-CGTase genetically engineered bacterium is subjected to 3L fermentor culture, foams produced in the fermentation process are obviously decreased, a mirror does not detect spore production, almost no extracellular amylase and protease exist in the culture process, after 70h of fermentation culture, the beta-CGTase enzyme activity reaches 270U/ml, and DCW reaches 70g/L.

The invention relates to a high-efficiency ferment for fermenting bean pulp, belonging to the biologic enzymolysis fermentation technical field. The high-efficiency ferment is formed by mixing bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus casei, saccharomyces cerevisiae and candida utilis. The fermentation technology comprises the following steps: crushing the bean pulp and corn; activating the strains; uniformly mixing the bean pulp and the fermentation liquor using a mixer; packing the mixed bean pulp in fermentation bag; fermenting the packaged bean pulp and processing fermented bean pulp: drying-crushing-detecting-packaging-finished product. The technology is simple and the product quality is stable and the content of the anti-nutrient factor in the bean pulp is eliminated and obviously reduced, therefore the bean pulp is a safe plant protein feeder for infant animal with balanced nutrient, good quality and low price and help for health growth of the infant animal, in replace of animal protein feeder of bone powder, fish powder or the like.

Fungal/bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal/bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal/bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The present invention relates to a method for enhancing the health of an animal comprising administering to the animal a composition comprising Bacillus subtilis QST 713 or a mutant thereof.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.