310 results about "Subtilis bacillus" patented technology

This invention relates to the field of pet nutrition technology, and more particularly to a fresh dog food for improving coat health and its preparation method. The fresh dog food of this invention comprises component A and component B. Component A includes the following components in parts by weight: 40-45 parts venison, 10-12 parts mussels, 8-10 parts chicken liver, 8-10 parts duck egg, 3-5 parts carrot, 3-5 parts broccoli, 3-5 parts pumpkin, 3-5 parts taro, 3-5 parts avocado, 0.8-1 part dried red dates, 0.8-1 part dried rose petals, 0.5 parts whey calcium, 1.3 parts malt powder, 0.5 parts inulin, 0.5 parts brewer's yeast, and 0.2 parts fish oil. Component B is Bacillus subtilis; the viable count of Bacillus subtilis is 1×10⁻⁶. 9 CFU / g. This invention utilizes the synergistic effect of deep-sea shellfish and venison to improve the coat quality of puppies, increasing the coat diameter by ≥23.1% and the scale thickness by ≥32.4%.

The present application relates to the technical field of microbial preparation, in particular to a compound microbial inoculant for selenium-enriched rice, a preparation method and application thereof.The compound microbial inoculant comprises selenium-reducing LYB-S bacterial liquid, bacillus subtilis bacterial liquid, xanthan gum, nano zinc oxide, nano iron oxide and water; wherein, in terms of mass percentage, the addition amount of the selenium-reducing LYB-S bacterial liquid is 50%, the addition amount of the bacillus subtilis bacterial liquid is 25%, the addition amount of the xanthan gum is 0.3%, the addition amount of the nano zinc oxide is 2%, the addition amount of the nano iron oxide is 1%, and the balance is water, and the total mass percentage is 100%. The compound microbial inoculant can be used to increase the selenium content of rice. In addition, the compound microbial inoculant can also promote the growth of rice simultaneously, increase the number of grains per panicle and panicle weight, and realize yield increase.

The application provides a promoter capable of improving the transcription level of a glycine nucleotide transcarbamylase gene and application, the promoter is a mutation based on the sequence shown in SEQ ID NO: 1, and two promoters capable of enhancing the transcription level of the glycine nucleotide transcarbamylase gene and capable of improving the expression of an alpha-amylase amyE gene from Bacillus subtilis subsp. subtilis str. 168 in coryneform bacteria are obtained.

A fermented traditional Chinese medicine feed additive for catfish, its preparation method, and its application are disclosed, relating to the field of aquatic feed additives. The preparation method involves activating and expanding the culture of *Saccharomyces cerevisiae*, *Bacillus subtilis*, and *Lactobacillus plantarum*; pulverizing *Paeonia suffruticosa* leaves, *Smilax china*, *Scutellaria baicalensis*, *Rhus chinensis*, hawthorn, *Polygonum cuspidatum*, ginkgo, and forsythia suspensa* to obtain traditional Chinese medicine powders; preparing a solid fermentation medium using the traditional Chinese medicine powders, soybean meal powder, corn cob granules, and wheat bran; inoculating *Saccharomyces cerevisiae* seed culture and *Bacillus subtilis* seed culture for aerobic fermentation; and inoculating *Lactobacillus plantarum* seed culture for anaerobic fermentation to obtain the fermented traditional Chinese medicine feed additive. The resulting fermented traditional Chinese medicine feed additive promotes the absorption and metabolic elimination of enrofloxacin in catfish, shortens the withdrawal period, has preventive and adjunctive therapeutic effects on bacterial diseases in catfish, promotes growth, reduces feed conversion ratio, and improves survival rate.

The application discloses a method for removing impurities from cow urine and application thereof in preparation of algal culture solution, and relates to the technical field of biological treatment. The method for removing impurities from cow urine adopts a multistage treatment system of chelating agent removal, activated carbon adsorption, physical filtration, composite microbial fermentation and precision filtration; the physical filtration adopts a stainless steel filter screen; the microbial fermentation adopts a composite microbial agent containing bacillus pasteurii, bacillus subtilis and lactobacillus plantarum; and the precision filtration adopts a microporous filter membrane. The application develops an efficient, environment-friendly and low-cost method for removing impurities from cow urine, removes macromolecular contaminant substances and harmful microorganisms and the like impurities in the cow urine, and meanwhile retains beneficial nutrient components in the cow urine; high-quality algal culture solution is prepared through the impurity-removed cow urine, the cost of the algal culture solution is reduced, and the nutrient components in the cow urine are fully utilized, so that abundant nutrient substances are provided for the growth of algae.

This invention belongs to the field of microbial technology, specifically relating to a compound microbial fertilizer that synergistically regulates rhizosphere flora and nitrogen metabolism genes and its application. The microbial agent includes: Bacillus belye (… Bacillus velezensis ), Bacillus subtilis subsp. subtilis ( Bacillus subtilis subsp. subtilis ) and Linyi Streptomyces ( Streptomyces linyiensis Two or three of the following. The microbial agent provided by this invention has significant growth-promoting and nitrogen-fixing effects when applied to crops. It can maintain or even improve soil fertility and crop yield under the condition of reducing nitrogen fertilizer application by 30%, providing a green and efficient new solution for the implementation of "nitrogen reduction and efficiency enhancement".

The present application relates to the technical field of feed processing, and particularly relates to a preparation method of Maotai-flavor distiller's grains fermented feed, which comprises the following steps: drying treatment based on the moisture content of Maotai-flavor distiller's grains to obtain basic distiller's grains; determining the strain ratio of brewer's yeast, bacillus subtilis and aspergillus niger based on the tannin content of the basic distiller's grains to obtain a compound fermentation inoculant; inoculating and then fermenting; determining state evaluation parameters based on the total polyphenol accumulation rate, the dissolved oxygen consumption rate and the microbial activity index during the fermentation process, and performing qualified evaluation, and dynamically adjusting when unqualified; determining the fermentation end point based on the tannin degradation rate and the total polyphenol accumulation rate; and mixing the fermented distiller's grains with the basic feed at a mass ratio of 1% after low-temperature drying of the fermented distiller's grains. The present application realizes three-layer control of raw material evaluation, process control and end point determination, significantly improves the stability and content of functional active ingredients in the product, reduces the tannin and safety risk, and has a good intestinal health improvement effect on animals.

This invention relates to the field of microbial technology, specifically to a compound bacterial agent, its preparation method, and its application, wherein the active ingredient is Bacillus arachidica (B. arachidica). Peanut Bacillus CH-RS16 and cold-resistant *Bacillus subtilis* ( Peribacillus frigoritolerans ZM-RS6. The compound microbial agent provided by this invention promotes the growth and development of wheat and improves its drought resistance.

The application provides application of a bacillus subtilis K8 nano-seleniumized active bacterium agent in prevention and treatment of plant pathogenic bacteria, and belongs to the technical field of biological prevention and treatment of plant diseases. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent is obtained by fermenting and culturing bacillus subtilis K8 in a fermentation medium containing sodium selenite. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent has a strong prevention and treatment effect on cucumber fusarium wilt, cucumber botrytis blight, tomato early blight and tomato root rot, provides an efficient, safe and practical method for prevention and treatment of plant fungal diseases, has low cost, simple preparation process, is convenient for industrialization, and has good development value and market prospect.

This invention belongs to the field of biological agents for aquaculture, and discloses a compound biological pond-cleaning agent containing tea saponin and its preparation method. The compound biological pond-cleaning agent of this invention comprises the following components by weight: 3-10 parts tea saponin, 60-70 parts roe beetle root powder, 10-20 parts Buddleja officinalis, 5-10 parts compound microbial powder, 3-8 parts adjuvants, and 10-20 parts carrier; the adjuvant is a mixture of sodium lignin sulfonate and sodium dodecylbenzene sulfonate; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria; and the carrier is diatomaceous earth. This invention combines three natural active ingredients—tea saponin, roe beetle root powder, and Buddleja officinalis—with suitable adjuvants and a carrier, which can expand the pond-cleaning range and has good killing or inhibitory effects on harmful organisms such as wild fish, shrimp, crabs, and snails, as well as various pathogens. The pond-cleaning is thorough, and the raw materials are easily degradable, leaving no residue. It is environmentally friendly and has a long-lasting effect, which is of great significance for promoting the green and sustainable development of aquaculture.

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.

The present application relates to the field of microbiological technology, and specifically provides a compound microbial flora preparation for improving crop straw compost humification and organic carbon fixation and application thereof. The compound microbial flora is composed of saccharophagus degradans CGMCC 1.10397, paenibacillus popilliae CGMCC 1.12900 and bacillus subtilis CGMCC 1.6757. Seed liquids of the above three strains are mixed and cultured in a volume ratio of 1:1:1 to obtain a compound microbial suspension, which is applied to the aerobic composting process of crop straw, can significantly improve the degradation efficiency of lignocellulose, promote the formation of humus and the stable fixation of organic carbon, thereby improving the compost quality, reducing the nutrient loss and greenhouse gas emission. The present application is suitable for the resource treatment of cotton straw, vegetable straw and other agricultural organic wastes.

This invention discloses a Tibetan bath composition compound and its preparation method, relating to the field of daily chemical products technology. The Tibetan bath composition compound consists of a ternary complex of scutellaria baicalensis hydroxypropyl-β-cyclodextrin nanoshell powder, Bacillus subtilis, Tibetan medicine composition, starch, anhydrous citric acid, sodium bicarbonate, calcium citrate, crospovidone, and magnesium stearate. Bacillus subtilis is used to ferment the ternary complex of scutellaria baicalensis hydroxypropyl-β-cyclodextrin nanoshell powder to obtain a fermentation-modified porous carrier, which is then loaded with the Tibetan medicine composition to form a Tibetan medicine inclusion powder. This not only achieves efficient drug delivery through a triple mechanism of biomodification, metabolite synergy, and structural optimization, but also forms a quaternary system with Terminalia chebula. The effervescent effect also enhances the penetration of Tibetan medicine components, which is beneficial for better exerting the antibacterial and anti-inflammatory effects of active substances, and significantly improving fungal infections and eczema-related skin problems.

This invention discloses a small-molecule soybean peptide, its preparation method, and its applications. The preparation method includes: mixing soybean protein isolate with water and then performing pH shift pretreatment, deep eutectic solvent pretreatment, and pulsed electric field pretreatment; subsequently, enzymatic hydrolysis, enzyme inactivation, centrifugation, and collection of the supernatant; then, performing a two-step fractional alcohol precipitation treatment on the supernatant, collecting the precipitate, reconstituteing, and freeze-drying to obtain the small-molecule soybean peptide. This soybean peptide has a molecular weight <1 kDa and a component content of over 94%, and can be used as a nitrogen source to replace soybean peptone in Bacillus subtilis fermentation medium. This small-molecule soybean peptide has a good yield-promoting effect on at least five Bacillus subtilis strains and can be used to promote the production of nattokinase by Bacillus subtilis biofilm fermentation, with the highest nattokinase activity reaching approximately 14800 U / mL.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

This invention belongs to the field of biotechnology and discloses a recombinant strain of Saccharomyces cerevisiae that enhances the efficient synthesis of vitamin K2 and its application. By heterologously integrating the hepS / T and naphthoquinone cyclotransferase genes (menA) of Bacillus subtilis, four genetically engineered strains were constructed based on the designed MK-7 biosynthetic pathway: YK-1, YK-2, YK-3, and YK-4. YK-1 is an expression strain containing the target genes MenF, MenD, and MenH; YK-2 is an expression strain integrating the target genes MenC, MenE, and MenB on the basis of YK-1; YK-3 is an expression strain integrating the target genes hepS and hepT on the basis of YK-2; and YK-4 is an expression strain integrating the target genes yuxO, MenA, and MenG on the basis of YK-3. YK-4 is the final engineered strain that produces high levels of vitamin K2. The optimal fermentation time was determined by measuring OD600 and yield curves. YK-4 reached its maximum cell concentration in 18 hours, which can effectively shorten the fermentation cycle.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

This invention belongs to the field of microbial pesticides and discloses a highly effective Bacillus subtilis strain for killing spider mites and its applications. The Bacillus subtilis strain described in this invention has the preservation number CCTCC NO: M 20251959. Indoor bioassay data show that Bacillus subtilis NBIF-051 has high toxicity against major agricultural mites such as the two-spotted spider mite, the citrus red spider mite, and the cotton red spider mite. Pot experiments showed that after spraying the fermented broth of NBIF-051 for 7 days, the control efficacy against the two-spotted spider mite was 65.18%. Field trials showed that after spraying the fermented broth of this strain for 15 days, the control efficacy against the citrus red spider mite was 63.45%~76.85%, comparable to commercially available chemical acaricides. Furthermore, the acaricidal activity of the fermented broth of this strain exhibits certain tolerance to high temperatures and ultraviolet radiation, showing broad application prospects in agricultural production.

The application discloses a process for improving the quality of palm kernel meal feed by enzyme and bacteria synergistic fermentation, and belongs to the field of feed biotechnology.The process is characterized by the following steps: crushing and screening palm kernel meal, mixing the palm kernel meal with 20% bran and 4% ammonium sulfate as dry matter to obtain a basic substrate;compounding bacillus subtilis, aspergillus niger and saccharomyces cerevisiae at a ratio of 1:1:1 of viable bacterial number, adding 0.67% mannanase and 0.3% xylanase to prepare a bacteria-enzyme mixed solution, inoculating the bacteria-enzyme mixed solution at a 10% inoculation amount, adjusting the moisture content to 50% and the pH to 6.4, and then sealing and storing the bacteria-enzyme mixed solution for use after 4 days of fermentation at 33.5 DEG C; and the bacteria-enzyme mixed solution can be stored for a long time.The process can efficiently degrade crude fiber and anti-nutritional factors, improve the protein content and the digestibility, is stable and easy to mass-produce, can replace part of corn and soybean meal in the daily ration of ruminants, and realizes high-value utilization of palm kernel meal.

The application belongs to the technical field of fertilizers, and provides a fertilizer based on SOD cultivation and a preparation method thereof.The preparation method comprises the following steps: S1, according to the weight fraction, 41-45 parts of urea, 26-28 parts of potassium chloride, 22-24 parts of calcium superphosphate, 5-7 parts of bacillus subtilis spore powder, 30-32 parts of rice husk charcoal, 3-5 parts of ammonium sulfate modified magnesium-aluminum hydrotalcite and 8-10 parts of calcium chloride modified attapulgite are put into a coating machine for stirring to obtain a mixture; S2, 5-7 parts of 4wt% polyvinyl alcohol aqueous solution and 3-4 parts of distilled water are added to the mixture obtained in S1 to accelerate the granulation of the material, and granular material is obtained; and S3, the granular material obtained in S2 is subjected to drying treatment, and the fertilizer based on SOD cultivation is obtained.The application can effectively reduce the nitrogen release speed of the prepared carbon-based microbial fertilizer.

The invention discloses a fattening feed additive composition for old cattle and a fattening method, and belongs to the technical field of feed. The additive composition comprises the following components in parts by weight: 13-20 parts of a rumen function regulator, 4-10 parts of an intestinal repair agent, 10-20 parts of a rumen bypass compound enzyme preparation, 5-10 parts of a muscle-increasing growth-promoting composition, 5.5-7.3 parts of a functional trace element composition and 32.7-62.5 parts of a carrier. Wherein the rumen function regulator is compounded by a coated saccharomyces cerevisiae culture and bacillus subtilis; the rumen bypass compound enzyme preparation comprises acid protease, neutral protease and cellulase; the muscle-building and growth-promoting combination is coated guanidinoacetic acid. Through regulation and control of a rumen-intestinal tract-tissue three-level linkage network, the rumen fermentation function of the old cattle is remarkably improved, the feed conversion rate is increased, and meanwhile muscle hyperplasia and meat tenderization are achieved. By means of the fattening method, the average daily gain of the old cattle is increased by 22.8% or above, the muscle shear force is reduced by 29.6% or above, the feed conversion ratio is reduced by 17.1% or above, and green and efficient fattening is achieved.

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

This invention relates to the field of feed additive technology, specifically to a Bacillus subtilis composition for improving animal intestinal health and its preparation method. It comprises the following raw materials: Bacillus subtilis engineered bacterial powder, cinnamaldehyde microcapsules, proanthocyanidins B2, selenium-enriched yeast culture, glycoterpenes, trehalose, and a carrier. The core of this invention lies in using Bacillus subtilis EhrBS-9 (CGMCC No. 24947) as the strain, constructing a dual-function engineered bacterium with tolerance and transformation through gradient cinnamaldehyde domestication and cad gene integration. This, combined with the intestinal-targeted delivery of cinnamaldehyde microcapsules, barrier strengthening of proanthocyanidins B2, and the construction of a multidimensional anti-stress network of selenium-enriched yeast, glycoterpenes, and trehalose, forms a synergistic mechanism of gut microbiota regulation, immune enhancement, and stress buffering. This addresses the industry pain point of insufficient synergistic effects of existing feed additives in terms of high-efficiency antibacterial activity, safe immune regulation, root-cause anti-stress, and ecological safety.

The application discloses a production process of high-potassium base compound fertilizer, which comprises the following steps: S1, raw material pretreatment: crushing potassium feldspar to 200 meshes, mixing with 5% sulfuric acid at a mass ratio of 1:3, calcining at 120-130 DEG C for 2-3 hours, washing until the pH is 6.5-7.0, and drying to obtain potassium feldspar activated powder; S2, putting potassium citrate solution, calcium citrate solution, potassium feldspar activated powder, trace element organic acid salt solution, urea, modified zeolite and binder into a high-speed mixer in proportion, uniformly stirring, adopting high-tower granulation to obtain base material particles; S3, drying the base material particles, removing the particles with a diameter less than 2.5 mm, spraying a plurality of composite films on the surface of the base material particles to coat, and drying to obtain coated base material particles; and S4, spraying bacillus subtilis agent on the surface of the coated base material particles, and low-temperature drying to obtain the high-potassium base compound fertilizer. The high-potassium base compound fertilizer produced by the application has high nutrient components, long fertilizer efficiency, small environmental pollution, and can improve the soil environment and improve the crop yield.

The application discloses a method for preparing a fulvic acid type liquid microbial fertilizer through minute-level composting of breeding wastewater, which comprises the following steps: adding biochar A, potassium ferrate and potassium sulfite into pig farm breeding wastewater, stirring for 10-20 minutes, and uniformly mixing to obtain liquid fertilizer B rich in fulvic acid-like substances; and compounding bacillus subtilis, DX-9 and AM microbial agents into the liquid fertilizer B to prepare a fulvic acid liquid microbial fertilizer C. The method uses biochar, potassium ferrate and potassium sulfite to realize rapid composting of the breeding wastewater within 10 minutes, and prepares a high-efficiency liquid fertilizer rich in fulvic acid; and the post-compounding of growth-promoting microbial agents (including bacillus subtilis, DX-9 and AM microbial agents) can enhance the fertilizer efficiency and improve the disease resistance of crops. The method has the advantages of high treatment efficiency and small occupied area, realizes high-value utilization of livestock and poultry breeding wastewater, can effectively promote crop growth, and helps the green and healthy development of the livestock industry, and has wide application and industrialization prospects in the fields of agriculture, forestry and animal husbandry.