2509 results about "Subtilis bacillus" patented technology

The invention discloses a microbial compound bacterial fertilizer for improving saline alkali soil and increasing the acting yield. A preparation method of the microbial compound bacterial fertilizer comprises the following steps: preparing a microbial agent containing phosphate-solubilizing bacteria, potassium-solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis and actinomycetes; the preparation method comprises the following steps: preparing a sodium alginate solution, adding nano-hydroxyapatite and a microbial agent, uniformly mixing, dropwise adding a calcium chloride solution, and embedding an inner layer to obtain microbial embedded particles; uniformly mixing biochar, humic acid and attapulgite, taking a 2-3% HPMC solution as a binder, and carrying out middle-layer coating, so as to obtain prefabricated microbial fertilizer particles; and coating the chitosan-sodium alginate-nano ZnO outer layer to obtain the microbial compound bacterial fertilizer. The microbial compound bacterial fertilizer disclosed by the invention adopts a multi-layer and multifunctional collaborative design concept, realizes efficient protection and slow release of functional microorganisms in a saline-alkali environment, remarkably improves the physicochemical properties of soil and promotes the growth of crops.

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention belongs to the technical field of soil fertilizers, and particularly relates to a soil conditioner and a preparation method thereof. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 30 to 40 parts of modified charcoal, 20 to 30 parts of microbial complexing agent, 15 to 25 parts of humic acid and 1 to 5 parts of biological enzyme; according to the microbial complexing agent, dopamine grafted and modified nano silicon dioxide and hydroxyapatite form a composite shell, pseudomonas aeruginosa and bacillus subtilis are wrapped, and the microbial complexing agent has the functions of protecting microorganisms, adsorbing heavy metals and slowly releasing phosphorus elements; the modified biochar is compounded with the attapulgite through acid-base treatment to form a porous structure with physical inlaying and chemical functional groups synergistic, so that the adsorption and fixation capability on heavy metals and organic pollutants is remarkably improved; the biological enzyme accelerates organic phosphorus conversion, and enzymatic hydrolysate provides a phosphorus source for microorganisms, promotes microorganism breeding, effectively increases the content of available phosphorus in soil, and enhances the stability of a remediation system.

The invention provides a special charcoal-based slow-release fertilizer for sandy soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The special charcoal-based slow-release fertilizer for the sandy soil is prepared from amino modified biomass charcoal, urea, urea formaldehyde, monoammonium phosphate, potassium sulfate, polyglutamic acid, fulvic acid, clay, attapulgite, a compound microbial agent and sodium alginate. The compound microbial agent is prepared from halomonas (ACCC 11681), bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094), and the compound microbial agent is prepared from bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094). The invention further provides a preparation method and application of the special charcoal-based slow-release fertilizer for the sandy soil. The special charcoal-based slow-release fertilizer for the sandy soil can remarkably improve the water and fertilizer retention capacity of the sandy soil, improve the farmland productivity of the sandy soil, save fertilizer and water input and improve the yield and quality of crops.

The invention discloses a multifunctional low-temperature corn straw degradation complex microbial inoculant, a preparation method and application, the complex microbial inoculant is compounded by a plurality of microorganisms with straw degradation, potassium dissolving and growth promoting functions, and the complex microbial inoculant comprises the following strains: bacillus subtilis K15, curveolate Prickia L11 and bacillus mycoides L31. The complex microbial inoculant disclosed by the invention realizes synergistic interaction and functional complementation, and through scientific compounding of strains, the straw decomposition rate is remarkably increased, and the decomposition period is obviously shortened; the adaptive capacity and colonization competitiveness of microorganisms in a low-temperature and complex soil environment in winter are enhanced. The complex microbial inoculant disclosed by the invention also has multiple functions of degrading straws, dissolving potassium, producing IAA (Indoleacetic Acid) and the like. The soil potassium which is difficult to utilize can be converted into quick-acting potassium while the straws are quickly decomposed to release nutrients.

The invention provides lactobacillus crispatus, which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 7, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 26503. The bacillus subtilis can play a relatively strong role in inhibiting pathogenic bacteria such as gardnerella vaginalis, escherichia coli and staphylococcus aureus at the same time, and also has a certain role in inhibiting candida albicans, two-way proteurella and Atortoria vaginalis. Therefore, the lactobacillus crispatus has the potential of maintaining the environment in the vagina to be faintly acid and improving the mucosal immunity and infection resistance of a host, and can be used for preventing and treating vaginal infection and inflammation, and maintaining, improving or recovering the steady state of the environment in the vagina.

The invention discloses a ruminant feed capable of replacing soybean meal as well as a preparation method and application of the ruminant feed. The ruminant animal feed is prepared from the following raw material matrixes in parts by weight through compound probiotic fermentation: 45-55 parts of corn sugar residues, 15-25 parts of corn steep liquor, 10-20 parts of corn germ meal, 3-8 parts of extruded soybeans, 3-8 parts of corn protein powder, 2-4 parts of urea and 0.5-1.5 parts of lysine. The compound probiotics are prepared from saccharomyces cerevisiae, lactobacillus plantarum and bacillus subtilis. The invention further discloses a preparation method of the ruminant feed and application of the ruminant feed in completely replacing soybean meal in daily ration of ruminants. The ruminant feed disclosed by the invention can completely replace soybean meal in daily ration of ruminants, and even if the ruminants are in a fattening period, the soybean meal does not need to be additionally added to maintain protein supply, so that the feed cost is effectively reduced, and multiple targets of saving cost, improving efficiency and protecting environment are achieved.

The invention relates to the field of agricultural microbial preparations, in particular to a complex microbial inoculant sustained-release granule for preventing and treating sweet potato stem nematode disease and a preparation method of the complex microbial inoculant sustained-release granule. Comprising the following components in parts by weight: 15-25 parts of paecilomyces lilacinus viable bacterium powder, 10-20 parts of bacillus thuringiensis viable bacterium powder, 10-20 parts of streptomyces viable bacterium powder, 5-15 parts of bacillus subtilis viable bacterium powder, 15-25 parts of charcoal, 5-15 parts of sodium alginate, 5-15 parts of humic acid, 5-10 parts of chitosan, 3-8 parts of calcium silicate, 1-5 parts of mannitol and 1-5 parts of sodium lignin sulfonate. Four microorganisms with complementary functions, namely paecilomyces lilacinus, bacillus thuringiensis, streptomycete and bacillus subtilis, are adopted, the sweet potato stem nematode disease is synergistically controlled through multiple action mechanisms, and the control effect and the stability are remarkably improved.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

The invention discloses a method for increasing the content of pyrazines and 2, 3-butanediol substances through high-temperature Daqu micro-ecological regulation and control, and belongs to the technical field of microbial food processing. The invention discloses a method for improving the content of pyrazines and 2, 3-butanediol substances through micro-ecological regulation and control of high-temperature yeast for making hard liquor, and the method comprises the following steps of: combining and inoculating strains, namely bacillus subtilis CGMCC (China General Microbiological Culture Collection Center) 10830, bacillus velezensis CGMCC 7044 and bacillus licheniformis CGMCC 20675, into wheat flour according to a proportion to prepare the high-temperature yeast for making hard liquor. According to the invention, not only are the contents of pyrazine substances and 2, 3-butanediol increased, but also the abundance of beneficial microorganisms such as bacillus, thermophilic fungi and the like is remarkably improved, and a good saccharification fermentation agent is provided for brewing high-quality white spirit.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses a composition with high ultraviolet damage resistance as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw materials. The composition with high ultraviolet damage resistance is prepared by performing solid-state fermentation on radix scutellariae roots, radix ligustici sinensis and radix puerariae through compound bacteria consisting of bacillus subtilis, lactobacillus bulgaricus and bacillus coagulans and then performing solvent extraction. The composition with high ultraviolet damage resistance has excellent effects of resisting oxidative damage and inhibiting inflammatory factors, and can achieve the effects of repairing skin barriers, resisting inflammation, relieving, efficiently promoting repair of after-sun skin and the like.

The invention relates to a fusion protein, an immunogenic composition and a recombinant degenerate vaccine for preventing and treating infection of various pathogenic streptococci, as well as a molecular architecture design, application and the like. According to the invention, three immune antigens, namely an elongation factor Tu (Tuf), a molecular chaperone DnaK and an elongation factor G (fusA), are screened, and it is proved that fusion protein molecules of the three antigens can significantly inhibit tissue lesions caused by infection of different serotypes and different types of streptococci, have good immunogenicity, play roles in immune protection and effective prevention and treatment, and have good application prospects. The bacillus subtilis has the characteristics of broad-spectrum and high-efficiency prevention of streptococcus bacterial infection, and has a wide application prospect.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention provides bacillus subtilis, fermentation liquor prepared from the bacillus subtilis and application of the fermentation liquor. The invention provides bacillus subtilis MPEB0002969, the bacillus subtilis is preserved in Guangdong Microbial Culture Collection Center (GDMCC), and the preservation number is GDMCC No: 63439. The invention further provides fermentation liquor prepared from the bacillus subtilis and application of the fermentation liquor in preparation of cosmetics. The fermentation liquor prepared from the bacillus subtilis provided by the invention has antioxidant, whitening and moisturizing activity, and can be used for preparing cosmetics.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

The invention belongs to the technical field of biology, and particularly relates to a preparation method and application of a rose extract, and the content of water-soluble vitamins in roses is increased by utilizing a microbial fermentation technology. According to the method, strains including lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are selected, the proportion of the strains is adjusted, the viable count proportion of the lactobacillus plantarum to the saccharomyces cerevisiae to the bacillus subtilis is (1-3): (1-2): (1-2), a complex microbial inoculant is formed, and pretreated roses are added for segmented fermentation. And finally, the content of water-soluble vitamins in the rose extract is increased, so that the total antioxidant activity is increased by about 35% while rose polysaccharide substances are retained. The rose extract is suitable for providing raw materials for functional food, health care products and cosmetics.

The invention belongs to the technical field of biological control, and particularly relates to biological control fermentation liquor and a preparation method and application thereof, the preparation method comprises the following steps: S1, raw material treatment: selecting solidago canadensis as a plant raw material, cutting the solidago canadensis into pieces, performing autoclaved sterilization, adding water, and mixing to obtain a mixture; s2, adding the cultured bacillus subtilis and saccharomyces cerevisiae into the mixture in the step S1, mixing and inoculating, and stirring and fermenting to obtain a fermentation stock solution; s3, centrifuging the fermentation stock solution, taking supernate, and filtering to obtain the biological control fermentation liquor. The biological control fermentation liquor prepared by the invention can be used for controlling seed germination, seedling growth and flowering processes of solidago canadensis, and meanwhile, the biological control fermentation liquor can also be used for preparing products for controlling solidago canadensis. The problem that a biological medicament for preventing and treating solidago canadensis is lacked at present is solved.

The invention provides a genetically engineered bacterium and a construction method of the genetically engineered bacterium, and the genetically engineered bacterium is characterized in that a green fluorescent protein sfGFP is used for mediating protein glutaminase, PG (protein glutaminase), PGF (protein glutaminase), PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF and PGF; eC 3.5. 1.44) is efficiently secreted and expressed in engineering bacteria, and belongs to the technical field of biological engineering. The method comprises the following steps: an obtained sfGFP gene is derived from an NCBI database (GenBank numbering: CP035486.1), an obtained PG zymogen gene PP (Propeptide-Protein glutaminase) is derived from a Chryseobacterium prion prgA gene (GenBank numbering: AB046594.1) in the NCBI database, a recombinant plasmid pHT01 / sfGFP-PP is introduced into bacillus subtilis WB800N to obtain an engineering bacterium, extracellular secretion expression of PP is realized, the sfGFP-PP is activated by trypsin to obtain PG, and the PG is subjected to enzyme activation to obtain the recombinant bacillus subtilis. The expression quantity and the secretion efficiency of the PG in the bacillus subtilis are greatly improved. The recombinant PG produced by the method can generate 23.5 U / mL enzymatic activity under activation of trypsin, can be used for improving protein functional characteristics, and has relatively high industrial production and application values.

The invention discloses a saline-alkali soil improvement and disease resistance double-effect controlled release fertilizer and a preparation method thereof. The fertilizer comprises 18-22% of biochar, 12-18% of furfural residues, 8-12% of cow dung compost and 6-10% of potassium humate. 5-7% of aluminum sulfate, 4-6% of sulfur and 3-5% of zeolite; the microbial inoculum comprises the following components in percentage by weight: 1.5 to 2.5 percent of saline-alkaline bacillus, 0.8 to 1.2 percent of bacillus subtilis and 0.8 to 1.2 percent 8-12% of a sodium polyacrylate coating, 4-6% of a sulfur coating and 1-3% of polyglutamic acid; 0.8 to 1.2 percent of humic acid chelated iron, 0.8 to 1.2 percent of algal polysaccharide and 0.8 to 1.2 percent of urease inhibitor; 0.8 to 1.2 percent of zinc sulfate, 0.5 to 1.0 percent of manganese sulfate and 0.5 to 1.0 percent of borax. The fertilizer can reduce the pH and conductivity of soil, increase the organic matter content of the soil and effectively improve the physicochemical properties of the soil, meanwhile, by adding the microbial agent, the disease resistance of crops can be effectively improved, the morbidity and disease index of the crops can be reduced, and nutrient release of the fertilizer has a sustained and controlled release characteristic; the initial release rate and the cumulative release rate of nitrogen are both lower than those of common chemical fertilizers, and the cumulative release rate of nitrogen is moderate within 60 days, so that the nutrient requirements of crops in different growth stages can be met.