Liquid fermentation process of streptomyces flavus and its production equipment

A liquid fermentation technology of Streptomyces flavinus, applied in the direction of bacteria, botanical equipment and methods, biocides, etc., can solve the problems of no production process and production equipment, small and insignificant number of Streptomyces flavinus, and avoid expansion The effect of loss, low production cost and high production efficiency

Inactive Publication Date: 2003-09-17
青岛地恩地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the amount of Streptomyces flavinus growing naturally in soil is too small to be insignificant
At present, there is no artificial production of such strains in China, and there is no corresponding production process and production equipment.

Method used

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  • Liquid fermentation process of streptomyces flavus and its production equipment
  • Liquid fermentation process of streptomyces flavus and its production equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1. A liquid fermentation process of Streptomyces flavinus. It includes the following steps:

[0036] (1) Mixing of protein and carbohydrate raw and auxiliary materials: send protein and carbohydrate raw and auxiliary materials, water and sodium hydroxide into the material mixing tank, and mix while sending until uniformly mixed, so that the solid concentration of raw and auxiliary materials is 30 %, the pH value of raw and auxiliary materials is 9.0;

[0037] (2) Disinfection of empty and solid seed cans: first sterilize the empty cans, then put raw and auxiliary materials into the sterilized solid cans, and sterilize with superheated steam at a temperature of 115°C for 40 minutes;

[0038] (3) Inoculation: after the solid tank is sterilized, cool the raw and auxiliary materials with pipeline cooling water, and when it reaches 30°C, inoculate the seeds of Streptomyces flavinus by 1% of the mass ratio of the raw and auxiliary materials;

[0039] (4) Seed tan...

Embodiment 2

[0046] Embodiment 2. A liquid fermentation process of Streptomyces flavinus. It includes the following steps:

[0047] (1) Mixing of protein and carbohydrate raw and auxiliary materials: send protein and carbohydrate raw and auxiliary materials, water and sodium hydroxide into the material mixing tank, and mix while sending until uniformly mixed, so that the solid concentration of raw and auxiliary materials is 33 %, the PH value of raw and auxiliary materials is 8.7;

[0048] (2) Disinfection of empty and solid seed cans: first sterilize the empty cans, then put raw and auxiliary materials into the sterilized solid cans, and sterilize with superheated steam at a temperature of 126°C for 35 minutes;

[0049] (3) Inoculation: after the solid tank is sterilized, cool the material with pipeline cooling water, and when it reaches 26°C, inoculate the seeds of Streptomyces flavinus by 1.7% of the mass ratio of raw and auxiliary materials;

[0050] (4) Fermentation in seed tanks: t...

Embodiment 3

[0057] Embodiment 3. A liquid fermentation process of Streptomyces flavinus. It includes the following steps:

[0058] (1) Mixing of protein and carbohydrate raw and auxiliary materials: send protein and carbohydrate raw and auxiliary materials, water and sodium hydroxide into the material mixing tank, and mix them while sending until they are evenly mixed, so that the solid concentration of raw and auxiliary materials is 35 %, the PH value of raw and auxiliary materials is 8.5;

[0059] (2) Sterilization of empty and solid seed cans: first sterilize the empty cans, then fill the raw and auxiliary materials into the sterilized solid cans, and sterilize with superheated steam at a temperature of 120°C for 30 minutes;

[0060] (3) Inoculation: after the solid tank is sterilized, cool the material with pipeline cooling water, and when it reaches 28°C, inoculate the seeds of Streptomyces flavinus by 2% of the mass ratio of raw and auxiliary materials;

[0061] (4) Seed tank ferm...

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Abstract

A liquid fermenting process of xanthostreptomycete includes such steps as proportioning raw materials, disinfecting seeding tank at 115-126 deg.C for 30-40 min, inoculating seeds at 26-30 deg.C, fermenting in seeding tank at 28-30 deg.C for 40-48 hr, disinfecting fermentor, inoculating and fermenting. Its fermenting apparatus is composed of screw conveyer, mixing tank, aseptic air maker, seeding tank, fermentor, and discharge valve. Its advantages are high efficiency, less pollution, and simple process.

Description

technical field [0001] The invention belongs to the technical field of Streptomyces luteus fermentation, and more specifically relates to the liquid fermentation process of Streptomyces luteus and the improvement of production equipment thereof. Background technique [0002] Existing Streptomyces flavinus multiplies in the soil. During the growth process, this strain can dissociate a large amount of solidified nitrogen, phosphorus, and potassium in the soil that cannot be absorbed by crops, improve the utilization rate of chemical fertilizers, and effectively alleviate the negative effects of soil compaction caused by extensive use of chemical fertilizers. At the same time, it can generate a variety of growth hormones to promote the growth of crops and improve their resistance to stress. Streptomyces flavinus can also produce a variety of antibiotics to inhibit the growth of pathogens, which is very beneficial to the elimination of soil-borne pathogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C05F11/08C12N1/20
Inventor 张永升徐树建
Owner 青岛地恩地生物科技有限公司
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