Compound bactericide for ensiling maize straws

A technology of compound bacterial agent and corn stalk, applied in the field of silage, can solve the problems of narrow growth condition adaptation range, unsatisfactory inoculation effect, reduced acid-producing function, etc., achieves low cost, improves aerobic stability, and inhibits secondary fermentation. Effect

Active Publication Date: 2017-06-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purified single bacterial agent has the disadvantages of reduced acid production function during the purification process, narrow adaptability to growth conditions, difficulty in obtaining a dominant position in silage, and unsatisfactory inoculation effect
However, the simple mixed bacterial agent, due to its simple composition, and the cooperative relationship or antagonistic relationship between the constituent bacteria has yet to be confirmed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, Screening of Active Bacteria in Composite Bacteria Agent for Corn Stalk Silage

[0026] Prepare improved SL culture medium: mix 10g tryptone, 10g yeast powder, 10g glucose, 10g sucrose, 6g potassium dihydrogen phosphate, 2g ammonium citrate, 15g anhydrous sodium acetate, 1ml Tween 80, 0.58g magnesium sulfate heptahydrate , 0.03g ferrous sulfate heptahydrate, 0.11g manganese sulfate tetrahydrate and 100ml boiled corn water are mixed, add water to 1000ml, adjust pH to about 5.3 with acetic acid, and get final product. The production of the boiled corn water: take 4 fresh corns with skins, weighing about 1.5kg, add 2.5L deionized water, and boil until about 800ml.

[0027] Well-fermented cabbage kimchi was used as the source of screening bacteria. Take 45mL of the improved SL culture solution and put it into a 50ml blue cap bottle, add 5g of kimchi, and culture it statically at 30°C. After culturing for 72 hours, transfer the cultures with an inoculum size of ...

Embodiment 2

[0029] Embodiment 2, the preparation of composite bacterial agent for corn stalk silage

[0030] The strains used in this example are conventional strains in the art.

[0031] Lactobacillus parabuchneri, Lactobacillus brevis, Lactobacillus plantaru, Lactobacillus paracasei, Lactobacillus pentosus and Lactobacillus mucosae were inoculated into the MRS medium respectively, and kept static at 30°C. Place and cultivate for 48h, mix the above-mentioned Lactobacillus paracasei with 6.7ml of the above-mentioned Lactobacillus paracasei, 5ml of the above-mentioned pentose milk Bacteria, the above-mentioned mucosal lactobacillus of 1.6ml, make the living bacteria number percentage of lactobacillus parabrunii, lactobacillus breve, plantarum lactobacillus, paracasei lactobacillus, pentosus lactobacillus and mucosal lactobacillus in the described mixed fermented liquid be respectively 31.7%: 31.7%: 23.3%: 6.7%: 5%: 1.6%, and making the total number of viable bacteria in this mixed liquor ...

Embodiment 3

[0033] Embodiment 3, the preparation of composite bacterial agent for corn stalk silage

[0034] In the present embodiment, Lactobacillus parabuchneri JCM 12493 T , Lactobacillus brevis JCM 1170, Lactobacillus plantarum JCM 1149 T and Lactobacillus pentosus JCM 1158 T Both are preserved in the Japanese Microorganisms Conservation Center, Lactobacillus paracasei CGMCC No.1.3112 and Lactobacillus mucosaeCGMCC No.6733 are preserved in the China General Microorganisms Culture Collection Management Center.

[0035] Lactobacillus parabuchneri JCM 12493 T , Lactobacillus brevis JCM 1170, Lactobacillus plantaru JCM 1149 T , Lactobacillus paracasei CGMCC No.1.3112, Lactobacillus pentosus JCM 1158 T Lactobacillus mucosae CGMCC No.6733 and Lactobacillus mucosae CGMCC No.6733 were respectively inoculated into the MRS medium, and cultured at 30°C for 48 hours, 35ml of the above-mentioned Lactobacillus parabrucei fermentation broth, 20ml of the above-mentioned Lactobacillus breve fermen...

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Abstract

The invention relates to the field of ensiling and especially relates to a compound bactericide for ensiling maize straws. The lactobacillus compound bactericide is mainly applied to the ensiling of the maize straws. The active bacteria include Lactobacillus parabuchneri, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus pentosus and Lactobacillus mucosae. The lactobacillus compound bactericide provided by the invention has the advantages of stable composition, high adaptability, high growth speed and low cost; the inoculated fermentation effect is obvious; the fermented quality of the maize straw silage can be effectively increased; the yield of acetic acid is increased; the aerobic stability is increased; the secondary fermentation is restrained; the dry material loss is reduced; and the lactobacillus compound bactericide has wide market development prospects and high application and promotion values in the ensiling field.

Description

technical field [0001] The invention relates to the field of silage, in particular to a composite bacterial agent for corn stalk silage. Background technique [0002] In recent years, with the development of industrial biogas projects in my country, the demand for anaerobic fermentation raw materials has gradually increased. There is a large amount of unused crop straw in our country every year. Crop straw is a good raw material for anaerobic fermentation. Anaerobic fermentation of crop straw by microorganisms to produce biogas is an important biomass energy conversion technology. Since the production of straw is seasonal, and the operation of industrial biogas projects requires a stable and continuous supply of raw materials, the preservation of raw materials is very important. [0003] There are mainly two ways to store crop straw: air-drying and silage. The air-drying process will result in the consumption of soluble matter, increased fibrosis, increased lignin content...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/225C12R1/24C12R1/25
CPCC12N1/20
Inventor 崔宗均张环王小芬袁旭峰朱万斌
Owner CHINA AGRI UNIV
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