Compound bactericide for ensiling maize straws
A technology of compound bacterial agent and corn stalk, applied in the field of silage, can solve the problems of narrow growth condition adaptation range, unsatisfactory inoculation effect, reduced acid-producing function, etc., achieves low cost, improves aerobic stability, and inhibits secondary fermentation. Effect
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Embodiment 1
[0025] Example 1, Screening of Active Bacteria in Composite Bacteria Agent for Corn Stalk Silage
[0026] Prepare improved SL culture medium: mix 10g tryptone, 10g yeast powder, 10g glucose, 10g sucrose, 6g potassium dihydrogen phosphate, 2g ammonium citrate, 15g anhydrous sodium acetate, 1ml Tween 80, 0.58g magnesium sulfate heptahydrate , 0.03g ferrous sulfate heptahydrate, 0.11g manganese sulfate tetrahydrate and 100ml boiled corn water are mixed, add water to 1000ml, adjust pH to about 5.3 with acetic acid, and get final product. The production of the boiled corn water: take 4 fresh corns with skins, weighing about 1.5kg, add 2.5L deionized water, and boil until about 800ml.
[0027] Well-fermented cabbage kimchi was used as the source of screening bacteria. Take 45mL of the improved SL culture solution and put it into a 50ml blue cap bottle, add 5g of kimchi, and culture it statically at 30°C. After culturing for 72 hours, transfer the cultures with an inoculum size of ...
Embodiment 2
[0029] Embodiment 2, the preparation of composite bacterial agent for corn stalk silage
[0030] The strains used in this example are conventional strains in the art.
[0031] Lactobacillus parabuchneri, Lactobacillus brevis, Lactobacillus plantaru, Lactobacillus paracasei, Lactobacillus pentosus and Lactobacillus mucosae were inoculated into the MRS medium respectively, and kept static at 30°C. Place and cultivate for 48h, mix the above-mentioned Lactobacillus paracasei with 6.7ml of the above-mentioned Lactobacillus paracasei, 5ml of the above-mentioned pentose milk Bacteria, the above-mentioned mucosal lactobacillus of 1.6ml, make the living bacteria number percentage of lactobacillus parabrunii, lactobacillus breve, plantarum lactobacillus, paracasei lactobacillus, pentosus lactobacillus and mucosal lactobacillus in the described mixed fermented liquid be respectively 31.7%: 31.7%: 23.3%: 6.7%: 5%: 1.6%, and making the total number of viable bacteria in this mixed liquor ...
Embodiment 3
[0033] Embodiment 3, the preparation of composite bacterial agent for corn stalk silage
[0034] In the present embodiment, Lactobacillus parabuchneri JCM 12493 T , Lactobacillus brevis JCM 1170, Lactobacillus plantarum JCM 1149 T and Lactobacillus pentosus JCM 1158 T Both are preserved in the Japanese Microorganisms Conservation Center, Lactobacillus paracasei CGMCC No.1.3112 and Lactobacillus mucosaeCGMCC No.6733 are preserved in the China General Microorganisms Culture Collection Management Center.
[0035] Lactobacillus parabuchneri JCM 12493 T , Lactobacillus brevis JCM 1170, Lactobacillus plantaru JCM 1149 T , Lactobacillus paracasei CGMCC No.1.3112, Lactobacillus pentosus JCM 1158 T Lactobacillus mucosae CGMCC No.6733 and Lactobacillus mucosae CGMCC No.6733 were respectively inoculated into the MRS medium, and cultured at 30°C for 48 hours, 35ml of the above-mentioned Lactobacillus parabrucei fermentation broth, 20ml of the above-mentioned Lactobacillus breve fermen...
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