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Method for preparing ferulic acid and oligosaccharide by producing enzymes from Salicornia bigelovii straws fermented by Aureobasidium pullulans

A technology of Aureobasidium pullulans and ferulic acid, which is applied in biochemical equipment and methods, microorganism-based methods, fermentation, etc., can solve problems such as not much research, and achieve the effect of low land cost and low management cost

Inactive Publication Date: 2011-09-14
YANCHENG INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there are not many studies on how to separate ferulic acid from enzymatic hydrolysis solution, but the technology for separating oligosaccharides is relatively mature

Method used

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  • Method for preparing ferulic acid and oligosaccharide by producing enzymes from Salicornia bigelovii straws fermented by Aureobasidium pullulans
  • Method for preparing ferulic acid and oligosaccharide by producing enzymes from Salicornia bigelovii straws fermented by Aureobasidium pullulans
  • Method for preparing ferulic acid and oligosaccharide by producing enzymes from Salicornia bigelovii straws fermented by Aureobasidium pullulans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Analysis of enzyme production effect of Aureobasidium pullulans metabolizing Salicornia stalks

[0021] Fermentation medium: 20-80g / L Salicornia stalk treatment liquid, add 0.05-0.3% KH 2 PO 4 and 0.05-0.4% MgSO 4 ·7H 2 O and 0.005~0.04% of V B1 ;

[0022] Preparation process of Salicornia straw treatment solution: After drying and crushing, the Salicornia straw is sieved through a 20-60 mesh sieve (20-80g / L) → adjust the pH to 4.5-6.0 with 1-5% sulfuric acid solution → 30-90 ℃ heat preservation for 2h → colloid mill treatment for 5-25min;

[0023] Sterilize with high pressure steam at 121°C for 15-20 minutes.

[0024] Cultivation of mother species: at a temperature of 25-30°C, a shaker speed of 80-160r / min, and a liquid volume of 20-100mL / 250mL triangular flask, inoculate 4 pieces of Aureobasidium pullulans with a diameter of about 6mm of the mother species block, cultivated for 5d;

[0025] Fermentation conditions: at a temperature of 25-30°C, a shak...

Embodiment 2

[0032] Example 2 Optimization of Aureobasidium pullulans Metabolism Salicornia stalk Enzyme Production Medium

[0033] Medium I Add V to the treatment solution of Salicornia stalks B1 0.05~0.4g / L, 0.50~4.0g / L MgSO 4 ·7H 2 O and potassium dihydrogen phosphate.

[0034] Medium II Add V to the treatment solution of Salicornia stalks B1 0.05~0.5g / L, MgSO 4 ·7H 2 O 0.50~5.0g / L and yeast extract 0.50~5.0g / L.

[0035] Fermentation conditions: at a temperature of 25-30°C, a shaker speed of 80-160r / min, a liquid volume of 20-100mL / 250mL Erlenmeyer flask, an Aureobasidium pullulans inoculum of 2-10%, and culture for 4-10 days.

[0036] Effect of culture medium on enzyme production of Aureobasidium pullulans

[0037] It can be seen from Table 2 that the enzyme production by the fungus was significantly different when the tea salamander was fermented in different media. In the optimized medium composition, the activities of extracellular xylanase and ferulic esterase produced by A...

Embodiment 3

[0041] Example 3 Optimization of Fermentation Conditions for Aureobasidium pullulans Metabolism Salicornia stalk Enzyme Production

[0042] Fermentation condition I pH5.0, liquid volume 50mL and inoculum volume 6%.

[0043] Fermentation condition II pH5.5, liquid loading volume 60mL and inoculum volume 12%, fermentation temperature is 25 ℃.

[0044] Fermentation medium wheat bran treatment liquid added V B1 0.14g / L, MgSO 4 ·7H 2 O 1.10g / L and yeast extract 2.50g / L. Effects of Fermentation Conditions on the Enzyme Production of Aureobasidium pullulans

[0045] Table 3 Fermentation of Aureobasidium pullulans to produce enzymes under different conditions

[0046] Table 3 Enzymes and activities pullulan during fermentation in different conditions

[0047]

[0048] From the enzyme production of Aureobasidium pullulans under different fermentation conditions (Table 3), it can be seen that culturing under optimized fermentation conditions can change the extracellular enzyme ...

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Abstract

The invention relates to application of ferulic acid and oligosaccharide preparation by producing enzymes from Salicornia bigelovii straws fermented by microorganism. The microorganism in the invention refers to Aureobasidium pullulans; the Salicornia bigelovii straws are dried and crushed to a certain particle diameter, and then a mixed enzyme preparation of xylanase and ferulic acid esterase is generated after the metabolism of the Aureobasidium pullulans; the cell walls of the Salicornia bigelovii straws are degraded under the synergistic effect of the mixed enzyme so as to obtain physiologically active substances, such as the ferulic acid and oligosaccharide. The invention has the effects of oxidation resistance, free radical elimination, thrombus resistance, blood fat reduction, coronary heart disease prevention and cure, bacteria resistance, inflammation elimination, mutation resistance and cancer prevention, can be widely applied in industries of medication, food and cosmetics, and can prominently increase the economic value of the straws, i.e., by-products of Salicornia bigelovii.

Description

technical field [0001] The invention relates to the application of salicornia stalks in the preparation of bioactive substances. Background technique [0002] According to the incomplete statistics of the United Nations Educational, Scientific and Cultural Organization (UNESCO) and the World Food and Agriculture Organization (FAO), there are 955 million hm2 of saline soil in the world. According to the latest research by the Nanjing Soil Institute of the Chinese Academy of Sciences, the total area of ​​various types of saline-alkali soil in my country has reached 99.13 million hm 2 . At the same time, there is a shortage of fresh water resources in the world, and the fresh water storage only accounts for 2.5% of the total water storage. Soil salinization and serious shortage of fresh water have become worldwide problems restricting agricultural development. In order to alleviate the conflict between man and land and make full use of seawater and saline soil resources, inte...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12P19/14C12R1/645
Inventor 邵荣余晓红张春银顾振新许伟肖玲陈洪兴刘珊珊
Owner YANCHENG INST OF TECH
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