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Application of samphire extract in inhibiting tyrosinase activity

A technology of salicornia extract and tyrosinase, which is applied in the field of application of salicornia extract to inhibit tyrosinase activity, and achieves the effect of low land cost and low management cost

Inactive Publication Date: 2011-01-26
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This o-benzoquinone is very passive and spontaneously forms melanin pigmentation, causing serious human aesthetic problems

Method used

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  • Application of samphire extract in inhibiting tyrosinase activity
  • Application of samphire extract in inhibiting tyrosinase activity
  • Application of samphire extract in inhibiting tyrosinase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The inhibitory effect analysis of embodiment 1 various extracts to tyrosinase activity

[0020] Preparation of Water (Alcohol) Extract of Salicornia officinalis

[0021] Take 3 g of ground Salicornia powder, add 60 mL of distilled water (or 60 mL of 60% ethanol by volume fraction) to soak for 24 hours, and reflux for 1 hour.

[0022] Preparation of Ultrasonic Water (Alcohol) Extract of Salicornia

[0023] Take 3 g of the ground samphire powder and add 60 mL of distilled water (or 60 mL of 60% ethanol) to soak for half an hour, boil for 30 min, cool to room temperature, and extract with ultrasound (59 kHz, 500 W, 65 ° C) for 1 h.

[0024] Determination of inhibition rate of tyrosine synthase activity

[0025] Take 2mL of the above-mentioned Salicornia extract, add 1mL of pH 6.8 phosphate buffer solution and 2mL of 2.0mmol / L tyrosine solution, after mixing evenly, quickly add 1mL of tyrosine synthase solution in an ice bath, mix well , and incubated at 37°C for 10min, ...

Embodiment 2

[0032] The inhibitory effect analysis of embodiment 2 different concentration modes to tyrosinase activity

[0033] Concentrate by distillation

[0034] The extract was centrifuged at 6000r / min for 4min to obtain a supernatant, which was placed in a boiling water pot until the liquid in the test tube evaporated to about 5mL.

[0035] Concentrate by rotary evaporation

[0036] The extract was concentrated and evaporated in a rotary evaporator at 60°C for 1 hour, and the mixture was centrifuged at 6000 r / min for 4 minutes to obtain a supernatant.

[0037] Depend on figure 2 It can be seen that the two different concentration conditions, evaporative concentration and rotary evaporator concentration of the extract, have little effect on the inhibition rate of tyrosine synthase activity. It shows that the components in the extract of Salicornia chinensis that inhibit the activity of tyrosine synthase are not significantly affected by temperature.

Embodiment 3

[0038] The compatibility effect of embodiment 3 ethanol is to the effect analysis of the salicornia extract inhibiting tyrosinase activity

[0039] From image 3 It can be seen that the salicornia alcohol extract concentrated by reflux with water has obvious inhibitory effect on tyrosine synthase, and compared with no extract, the enzymatic reaction time curve moves down; The reaction time curve continued to move down, indicating that due to the compatibility of ethanol, the inhibitory effect of salicornia extract on tyrosine synthase was more significant. In conclusion, the alcohol extracts of salicornia treated in two ways have obvious inhibitory effect on tyrosine synthase.

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Abstract

The invention relates to application of a samphire extract in inhibiting tyrosinase activity. The samphire extract is a concentrate obtained by respectively carrying out distilling concentration and the evaporating concentration of a rotary evaporator on a samphire water extract, a samphire alcohol extract, a supersonic water extract and a supersonic alcohol extract, and an inhibiting action research result of the samphire extract on the tyrosinase activity is provided. Proved by results, the distilling concentration and the evaporating concentration of the rotary evaporator hardly have influence on the inhibiting rate of the extract. The four extracts have obvious differences of the inhibiting action on tyrosinase using tyrosine as a substrate. The inhibiting rates of the water extract, the alcohol extract, the supersonic water extract and the supersonic alcohol extract are respectively 32.48 percent, 62.37 percent, 43.56 percent and 75.81 percent, wherein the samphire supersonic alcohol extract expresses best inhibiting action. The invention provides basis for preventing and treating pigmentation diseases.

Description

technical field [0001] The present invention relates to the application of salicoria extract in inhibiting tyrosinase activity. Background technique [0002] Since tyrosinase is the key to the synthesis of melanin, most of the current skin whitening products add tyrosinase inhibitors to cosmetics to achieve the purpose of reducing melanin in the skin by destroying the activity of tyrosinase. [0003] Hydroquinone used to be one of the most widely used whitening agents because of its cheap price and good effect. The mixed formula of hydroquinone and retinoic acid is quite effective in eliminating melanin deposition caused by ultraviolet rays and hormones. However, later studies showed that hydroquinone can induce leukemia. Since 2001, Europe has banned the addition of hydroquinone in whitening cosmetics. [0004] Kojic acid, azelaic acid, glycolic acid, niacinamide, arbutin and other chemicals can inhibit tyrosinase and are also used in whitening cosmetics. When some tyro...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K36/185A23L1/29A61Q19/02A61P17/00A23L33/00
Inventor 余晓红邵荣陈洪兴刘汉文许伟申玉香郑清张龙
Owner YANCHENG INST OF TECH
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