507 results about "Tyrosinase activity" patented technology

Compositions and methods are taught for directing the orientation of an immobilized capture biomolecule on a hydrophobic membrane. The method comprises layering at least one tie layer on a hydrophobic membrane, adding an amine functional layer on top of at least one tie layer; and attaching an alignment biomolecule to the amine functional layer. The alignment biomolecule has the ability to either capture a target biomolecule itself and thus be considered a capture biomolecule, or bind and orient the immobilized capture biomolecule so as to maximize the binding activity of the immobilized capture biomolecule. In one embodiment, a nickel-coordinated amine functional layer binds with a histidine-tagged alignment biomolecule. In another embodiment, an amine functional layer reacts, via tyrosinase catalysis, with a tyrosine residue in an alignment biomolecule.

The invention discloses a skin whitening composition which comprises a white mulberry root-bark extract, a chamomile extract, a rhodiola rosea extract, a grape seed extract, a licorice extract, a morus australis extract, arbutin, kojic acid dipalmitate, nicotinamide, tetrahydrodiferuloylmethane, a moisturizer and a preservative. The effective skin whitening ingredients of the skin whitening composition can inhibit the activity of tyrosinase, can block the transportation of melanin and can inhibit the formation of melanin; the skin whitening composition can be applied to skin whitening cosmetics and is safe and effective.

PCT No. PCT / KR98 / 00039 Sec. 371 Date Nov. 6, 1998 Sec. 102(e) Date Nov. 6, 1998 PCT Filed Mar. 2, 1998 PCT Pub. No. WO98 / 39279 PCT Pub. Date Sep. 11, 1998The present invention relates to a novel 1,3-diphenylpropane derivative having an inhibitory activity against tyrosinase represented by the following formula (I): in which +E,uns - -+EE represents double or single bond, R1 represents hydrogen or C1-C10 alkyl, R2 represents C1-C5 alkyl or C1-C5 alkoxyalkyl, or R1 and R2 together represent a 5 to 6 membered heterocycle which can be substituted by C1-C5 alkyl and which contains oxygen as the hetero atom, R3 represents hydrogen or C1-C7 alkyl, R4 represents hydrogen, hydroxy, or oxo, and R5 and R6 mindependently of one another represent hydrogen or C1-C5 alkyl.

The present invention relates to a method for detecting phenol compounds in a water body environment through an enzyme electrochemical biosensor. The biological sensor detection apparatus comprises a working electrode, an auxiliary electrode, a reference electrode, a detection cell and an electrochemical workstation. The specific operation steps comprise: adding a hydrophilic ionic liquid (such as 1-butyl-3-methylimidazolalanine and BMIM [Ala]) to a mesoporous carbon solution, and shaking for half an hour to obtain a novel mesoporous carbon and hydrophilic ionic liquid composite material; adding tyrosinase to the mesoporous carbon and hydrophilic ionic liquid composite material solution, shaking for one hour, and adding the obtained solution to the surface of a working electrode in a dropwise manner to obtain a tyrosinase immobilized working electrode; and inserting the tyrosinase immobilized working electrode, the auxiliary electrode and the reference electrode into a detection cell filled with a phosphate buffer solution, and adding a single standard substance of a phenol compound under a stirring condition to produce a corresponding current response signal.

The invention discloses rhodiola rosea fermentation protoplasm cosmetic and a preparation method thereof. Efficient ingredients of the rhodiola rosea fermentation protoplasm cosmetic are supernate obtained by mixing powder of rhodiola rosea root, water and zymophyte to obtain an initial system, fermenting the initial system to obtain zymotic fluid and sterilizing and centrifuging the zymotic fluid. The zymophyte is saccharomycete. The rhodiola rosea root is subjected to whole plant fermentation by adopting the saccharomycete, all functional ingredients and activity of the plant are kept, and loss of active ingredients caused by an extracting method is avoided. Any organic reagents are not added during extraction, fermentation temperature and fermentation pH are mild, structure of plant active ingredients is not damaged, natural activity of the plant is kept, and chemical components of essence and the like are not added in the fermentation protoplasm so as to ensure the safety of products on the human body. The rhodiola rosea fermentation protoplasm cosmetic is rich in salidroside, arbutin and the like having activities of restraining tyrosinase activity and restraining melanin synthesis so as to have a relatively strong whitening function on skin and has a synergistic effect with the components in the fermentation filtrate simultaneously.

The invention discloses whitening cosmetic and a preparation method thereof. A composition of a plurality of plants is taken as a main raw material, and the whitening cosmetic mainly comprises the following components: a rice bran extract, a rhodiola rosea extract, a ginkgo leaf extract, tea polyphenol, a lemon juice concentrated liquor, chitosan, a puerarin extract, konjac powder, a sunflower faceplate extract, a sunflower seed extract, a saponin kernel, hemicelluloses and honey. In addition, the whitening cosmetic also contains the following raw materials: a little of glycerinum, 1,3-propylene glycol, squalane, sodium hyaluronate, carbomer, triethanolamine, triethanolamine, allantoin, nanometer titania, collagen powder, an antibacterial agent, a ferulic acid isooctyl ester and glutathione. The composition can be applied to a whitening formula, has the effects of whitening the skin, moisturizing, resisting bacteria and diminishing inflammation, removing free radicals, inhibiting melanin formation, and inhibiting hallachrome mutase and tyrosinase activity.

This invention discloses skin darkening (sunless tanning) compositions based on enhancement of melanin synthesis by tyrosinase enzyme by providing essential substrates for tyrosinase as well as the essential elements to activate the active-site of tyrosinase enzyme. These compositions thus also act as promoters of tyrosinase enzyme.

The invention provides a preparation method and an application of a co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The preparation method comprises steps of: immobilizing tyrosinase and peroxidase on oxidized grephene to obtain a co-immobilized enzyme liquid and smearing dropwisely the co-immobilized enzyme liquid and a chitosan solution successively on a surface of a glassy carbon electrode to obtain the co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The electrode is low in cost, is easy and convenient to operate, is quick and sensitive, is low in detection limit, is good in selectivity and can be used for detecting microscale p-dihydroxybenzene and o-dihydroxybenzene in environment.

Novel double-stranded RNA oligonucleotides are useful for decreasing tyrosinase expression, have cosmetic and / or pharmaceutical applications, for example are useful skin depigmenting or anti-browning agents, and can be associated with cationic particles less than or equal to 1 μm in size, having a zeta potential of from 10 to 80 mV.

The invention discloses a composition with a skin whitening effect, and application thereof to cosmetics. The composition is prepared from the following ingredients in percentage by mass: 0.01 percent to 10 percent of 1-methylhydantoin-2-imide, 0.01 percent to 20 percent of nicotinamide, 0.01 percent to 20 percent of thyme extracts, 0.01 percent to 20 percent of orange peel extracts, 0.001 to 0.5 percent of fullerenes and the balance auxiliary materials and water. The composition has the advantages that the composition is safe and achieves the skin whitening effect; the irritability is low; free radicals can be effectively cleared away; efficient oxidation resistance is realized; the composition with the skin whitening effect is provided; the composition has different action mechanisms of inhibiting the tyrosinase activity, resisting oxidization, clearing away free radicals, brightening skin, holding moisture, accelerating the metabolism function of skin cells, and the like; the composition acts on the epidermal layer, the horny layer and the corium layer, so that the synergistic skin whitening effect is achieved; the irritation and the irritability are low.

The invention relates to a composition with the efficacies of whitening, moisturizing, repairing and caring the skin, as well as a preparation method and applications of the composition. The skin care composition is prepared from the following raw materials: water, sodium hyaluronate, sodium dihydrogen phosphate dihydrate, potassium chloride, sodium hydroxide, calcium chloride, glutamine, benzalkonium chloride, magnesium sulfate, aminobutyric acid, sodium ascorbate, alanine, arginine, lysine hydrochloride, valine, histidine, leucine, taurine, coenzyme A, alcohol, polysorbate 80, thiamine, disodium diphosphate, recombinant human epidermal cell growth factors and the like. The skin care composition disclosed by the invention can permeate the muscle bottom for replenishing water for the skin from the deep layer, so that the skin is full of elasticity, the color of the skin is brightened, the fine wrinkles are reduced, the skin is clean and watery, the balance of water and oil is regulated, the pores are shrunk, meanwhile, the brightness of the face can be increased, the injured epidermal layer is effectively repaired, the activity of tyrosinase is inhibited, the generation of skin melanin is reduced, so that the skin is healthy, natural and white.

The invention discloses whitening and skin care essence with synergistic whitening effects and a preparation method thereof. The whitening and skin care essence comprises niacinamide, a whitening complex 1, a whitening complex 2, citrus peel extract and arbutin. The whitening and skin care essence utilizes synergistic raw materials, can inhibit tyrosinase activity and melanin transfer, can brighten skin color, can cooperate in various nodes in the whitening path and can realize safe, effective and stable whitening effects.

The invention provides a traditional Chinese medicine composition with whitening efficacy, comprising the following bulk drugs, by weight: 10-30 parts of licorice, 10-25 parts of tree peony bark, 10-20 parts of fructus tribuli, 5-10 parts of polygonatum rhizome and 5-10 parts of motherwort. The compound traditional Chinese medicine extractive provided by the invention has a plurality of efficacies, such as absorbing ultraviolet ray, inhibiting tyrosinase activity, removing free radicals, promoting blood circulation, removing spot, whitening, smoothing skin and delaying aging, etc. Besides, the extractive is natural green, healthy and safe, so that the extractive can be used as a whitening agent and widely applied to cosmetic preparation, especially preparation of cosmetic with whitening efficacy.

The invention discloses a SERS sensor of a sandwich structure for detecting tyrosinase, and a preparation and detection method thereof. The SERS sensor utilizes the specific recognition principle of the tyrosinase antigen antibody, and a solid phase antibody-to-be-detected antigen-labelled antibody sandwich structure is formed through the combination of the solid-phase antibody on a substrate andthe labelled antibody with the antigen through the adsorption of the magnetic substrate and the enhancement effect of the surface-enhanced Raman probe, the content of the tyrosinase is analyzed by recognizing a labelled molecular SERS signal, and then a surface-enhanced Raman analysis method for detecting the tyrosinase is established. The detection method disclosed by the invention has the advantages of being quick, sensitive, simple and less in sample consumption. Furthermore, the sensor for detecting the tyrosinase has good linearity, the detection limit is low, and can reach 1ng / mL. And meanwhile, the adding standard recovery rate of the tyrosinase in the human serum sample is in the range of 96.0%-136.0%. Therefore, the SERS sensor is expected to be an effective method for clinicallydetecting tyrosinase.

Methods and formulations are provided to reduce pigmentation in skin, using an array of compounds selected from benzimidazoles, phenylthioureas, phenyltiols, phenylamines, bi- and multicyclic phenols, thiopheneamines, and benzothiamides. The compounds preferably inhibit pigment systhesis in melanocytes through the tyrosinase pathway. The methods can be used for lightening skin, and for treating uneven skin complexions which result from hyperpigmentation-related medical conditions such as melasma, age spots, freckles, ochronosis, and lentigo. The compounds can be used medically or cosmetically.

The invention relates to an experimental method for analyzing the toxicity and the effect of a skin whitening agent through a primary culture human being skin melanocyte. The method comprises the following steps: 1, culturing and identifying the primary human being skin melanocyte; 2, carrying out the analysis of the toxicity of skin whitening substances through the primary human being skin melanocyte; and 3, carrying out the analysis of the effect of the skin whitening substances through the primary human being skin melanocyte, namely carrying out the analysis on two aspects of the melanin content and the tyrosinase activity. The method has the following advantages that: the human being melanocyte cultured in vitro has a high division and multiplication capacity, a high standardization degree, little difference between batches, and the same function of producing melanin granules with the melanocyte in vivo; the result obtained through the healthy human being melanocyte is more reliable than the result obtained through animal or human-derived melanoma cells; and the toxic action and the whitening effect of the substances to be tested are evaluated from the two aspects of characterization and quantification. The method can replace the living body animal and human being skin, and can be directly applied to toxicity and effect experiments of the whitening substances in products such as chemicals, cosmetics, medicaments, and the like.

The invention discloses a modified-electrode-based tyrosinase biosensor comprising a glassy carbon electrode, wherein nanogold particles are deposited on the surface of a detection end of the glassy carbon electrode; an L-lysine film is adsorbed on the outer surfaces of nanogold particles; ordered mesoporous carbon supported nanogold is loaded outside the L-lysine film; and tyrosinase is adsorbed on the ordered mesoporous carbon supported nanogold. The preparation method comprises the following steps of: firstly preparing suspension liquid of the ordered mesoporous carbon supported nanogold, depositing the nanogold particles on the glassy carbon electrode by using an electrochemical method, immersing the detection end of the nanogold modified electrode in an L-lysine solution, forming a layer of L-lysine film on the outer surface of each nanogold particle by using the electrochemical method, dropwise adding the suspension liquid of the ordered mesoporous carbon supported nanogold to the surface of the detection end, drying, and dropwise adding a tyrosinase solution in air and so as to obtain the tyrosinase biosensor. The tyrosinase biosensor disclosed by the invention has the advantages of low cost, simpleness in manufacturing, long service life, high enzyme activity, high detection precision and efficiency and the like.

The invention discloses a preparation method and an application of a mulberry twig effective part with a tyrosinase inhibiting effect and belongs to the technical field of medicine and food. The mulberry twig effective part with the tyrosinase inhibiting effect contains five polyphenol compounds which are extracted from mulberry twigs and have tyrosinase inhibiting activity, and the five polyphenol compounds include oxyresveratrol, Steppogenin, Moracin M, 2,4,2',4'-tetrahydroxychalcone and mole Morachalone A. The mulberry twig effective part with the tyrosinase inhibiting effect can be effectively applied to inhibition for enzymatic browning of fruits and vegetables and skin whitening of cosmetics, used for preventing and treating human pigmentation diseases and melanoma caused by excessive melanin, and applied to various preparations of drugs for treating diseases requiring inhibition of tyrosinase activity.

The invention provides a stem cell exosome capable of repairing skin as well as a preparation method and application thereof. The preparation method comprises the following step: transducing decapeptide-12 into mesenchymal stem cell exosome, thereby obtaining the stem cell exosome capable of repairing skin. According to the stem cell exosome capable of repairing skin prepared by the method disclosed by the invention, the invention first discovers that, by binding the mesenchymal stem cell exosome and decapeptide-12 and by utilizing loading capacity and low immunogenicity of exosome cells, thedecapeptide-12 can reach melanocyte so as to further inhibit tyrosinase activity, the inflammatory response can be regulated, and skin related cell regeneration is promoted. The stem cell exosome capable of repairing skin prepared by the invention can achieve effects of eliminating erythema and edema when applied to sun-burned skin, can reduce melanogenesis and further reduce pigmentation and formation of black spots after sunburn, can be applied to the fields of cosmetics, medicines for treating skin diseases and the like, and has obvious effects and important application value.

The invention provides a quantitative realtime RT-PCR assay for detection of metastatic breast, gastric, pancreas or colon cancer cells or metastatic melanoma. The assay allows to predict disease recurrence and survival in patients with AJCC stage I and II, and III disease using multimarker panels. The method for detecting metastatic melanoma cells utilizes panels of markers selected from a group consisting of MAGE-A3, GalNAcT, MART-1, PAX3, Mitf, TRP-2, and Tyrosinase. The method for detecting metastatic breast, gastric, pancreas or colon cancer cells in paraffin-embedded samples utilizes panels of markers selected from a group consisting of C-Met, MAGE-A3, Stanniocalcin-1, mammoglobin, HSP27, GalNAcT, CK20, and β-HCG.

The invention relates to a spot-removing skin care product and a preparation method thereof. The spot-removing skin care product comprises the following raw materials: glycerin, stearic acid, glyceryl stearate, mineral oil, tocopherol, arbutin, angelica, atractylodes rhizome and bletilla striata root extract, lavender essential oil, spermol, trolamine and a preservative. The method comprises the following steps: the raw materials are melted in a melting tank, insulated and blended evenly in a vacuum homogeneous emulsion tank, cooled and stirred to obtain the semi-finished products; the semi-finished products are inspected, discharged and filled to prepare the finished products. The spot-removing skin care product of the invention contains diverse active ingredients having the functions of whitening, spot-removing and healing and effectively inhibits tyrosinase activity, thereby comprehensively inhibiting formation of melanin, accelerating metabolism, healing damaged skin, comprehensively caring skin, improving the metabolism function of skin cell and ensuring fast spot-removing effect, good effect and no toxic side effects on human bodies.

The invention discloses a yeast fermentation compound composition and an application thereof in whitening and moisturizing skincare products. The yeast fermentation compound composition is fermentation liquor obtained by adding a fragrant solomonseal rhizome extract and a jasmine flower extract into a yeast culture medium and performing fermentation by using yeasts. The appearance of the composition is clear and slight yellow, is unique in smell and has typical rich and pleasant fragrances of jasmine and fragrant solomonseal rhizome. The yeast fermentation compound composition is discovered to have a significant inhibitory action on tyrosinase activity and a good moisturizing effect. The fermentation compound composition can be used as an additive component, and is added into cosmetics to prepare external preparations according to a known method in the cosmetic industry, such as cosmetic dosage forms of solution, suspension, creams, emulsions, gels, powder, sprays and the like, thereby achieving effects of whitening and moisturizing.

The invention relates to an anoectochilus roxburghii flavone extraction process and application thereof in skin care products. The extraction process comprises the following steps of S1, conducting pulping, freeze drying and smashing on the anoectochilus roxburghii in sequence, and conducting extraction on the smashed anoectochilus roxburghii powder to obtain an anoectochilus roxburghii flavone crude extract liquid; S2, filtering the crude extract liquid obtained in the S1, reducing pressure and concentrating to obtain an anoectochilus roxburghii flavone crude extract; S3, conducting purification on the anoectochilus roxburghii flavone crude extract by adopting a Sephadex LH-20 gel column, so that an anoectochilus roxburghii flavone extract is obtained, wherein technological conditions in the S1 for freeze drying is a freezing temperature of 70-80 DEG C, freezing time of 40-60 hours and vacuum pressure of 15-25Pa. The provided extraction technology has high an extraction rate, and the anoectochilus roxburghii flavone extract obtained by extraction has high content of effective constituents, less impurities, and good antioxidant activity and tyrosinase activity inhibition.

The invention discloses an electrochemical biosensor for detecting bisphenol-A, and a preparation method and an application thereof. The electrochemical biosensor comprises a counter electrode, a reference electrode and a working electrode. The electrochemical biosensor is characterized in that the working electrode is a glassy carbon electrode surface-modified by a graphene / gold complex, tyrosinase and chitosan. The preparation method comprises a step of preparing the graphene oxide; a step of preparing the graphene / gold complex; and a step of polishing and cleaning a bare glassy carbon electrode. A three electrode system electrochemical enzyme sensor is formed in the way that the graphene / gold complex, the tyrosinase and the chitosan are modified on the glassy carbon electrode to be used as the working electrode, a platinum electrode is used as the counter electrode and a saturated calomel electrode is used as the reference electrode. The concentration of bisphenol-A in a to-be-detected sample is determined by immersing the electrochemical biosensor in the to-be-detected sample and according to a quantitative relationship between corresponding current value and the concentration of bisphenol-A. The electrochemical biosensor has high sensitivity, strong selectivity, high accuracy and fast detection speed. The preparation method is simple and practical, and convenient for operations in actual detection.

The invention belongs to the technical field of analysis and detection, and discloses a fluorescent probe and a preparation method thereof, and applications of the fluorescent probe in detection of tyrosinase, wherein the system of the fluorescent probe is named 3,3'-({5-[3-(4-hydroxyphenyl)ureido]-5H-benzo[a]phenoxazin-9-yl}azanediyl)methyl dipropionate, and the structure formula is represented by a formula (I). According to the present invention, the fluorescent probe compound can be used for the qualitative and quantitative analysis of tyrosinase, and has advantages of good detection precision, good accuracy and good anti-interference property. The formula (I) is defined in the specification.

The invention discloses a medicinal composition with an effect of removing chloasma. An oral preparation is prepared from the following active ingredients in percentage by weight: 10 to 50 percent of red-rooted salvia root extract, 10 to 40 percent of dangshen extract, 5 to 30 percent of rose extract, 5 to 30 percent of angelica dahurica extract, 3 to 25 percent of grape seed extract, 5 to 25 percent of vitamin C and 3 to 20 percent of vitamin E. The medicinal composition can effectively scavenge free radicals and restrain tyrosinase activity, thereby restraining the generation of melanin, achieving the effects of improving facial microcirculation, reinforcing facial nutrition and removing color sports and fulfilling the aim of removing chloasma; and the medicinal composition is good in curative effect, quick in response, low in price and free from toxic or side effect.

The invention discloses a preparation method of a common bombax flower extract with whitening and anti-aging activities. The preparation method is characterized in that dried common bombax flowers are crushed and sieved; the common bombax flower is subjected to ethanol soaking extraction twice; extracted liquids obtained in the two times are combined; reduced-pressure concentration is carried out, such that a suspension liquid is obtained; the suspension liquid is sequentially extracted by using petroleum ether and ethyl acetate; reduced-pressure concentration is carried out, and ethyl acetate is recovered; and a paste is obtained by vacuum drying, such that the common bombax flower extract is obtained. With the method, the prepared common bombax flower extract has anti-free radical oxidation, tyrosinase activity inhibiting, and anti-ultraviolet radiation functions, and can be used in skin whitening and aging-preventing especially photoaging-preventing. The common bombax flower extract can be used as a cosmetic additive or a medicine active component, and can be applied in whitening and anti-aging cosmetics.

The invention discloses a tuber fleeceflower root hair blackening formula, which comprises the following Chinese medicinal compositions in part by weight: 1 to 40 parts of tuber fleeceflower root, 1 to 40 parts of black sesame, 1 to 40 parts of rhizoma drynariae, 1 to 40 parts of yerbadetajo herba ecliptae, 1 to 30 parts of glossy privet fruit, 1 to 30 parts of south dodder seed, 1 to 30 parts of prunella spike, 1 to 30 parts of hiraute shiny bugleweed herb, 1 to 30 parts of mint, 1 to 30 parts of szechuan lovage rhizome and 1 to 30 parts of dried ginger. Two external formulations such as a lotion and a liniment / tincture can be prepared by adding a certain amount of water into the tuber fleeceflower root hair blackening formula or extracting with ethanol. Through the pathogenic wind dispersing, tonifying and blood circulation invigorating effects of the tuber fleeceflower root hair blackening formula, the activity of tyrosinase in hair follicles is enhanced, the synthesis of melanin is promoted for blackening hair and poliosis is improved. A plurality of raw materials are used together, so that blood circulation of scalp is promoted, the scalp, hair and hair follicle obtain sufficient nutrition, the regeneration of hair melanin cells is promoted, and white hairs becomes black from roots. Moreover, the tuber fleeceflower root hair blackening formula has the effects of removing scurf, stopping itching in head, washing hair and protecting hair.

The invention provides a tyrosinase activity detection method based on a recyclable SERS sensor. The method comprises the following steps of (a) providing the surface enhanced Raman scattering (SERS)) sensor, wherein the sensor is a self-assembled electrode, the electrode is provided with a nanogold particle membrane layer with SERS activity, and Raman signal molecules exist on the nanogold particle membrane layer; (b) establishing a tyrosinase activity detection fingerprint spectrum and a standard curve; (c) acquiring a surface enhanced Raman spectrum of the SERS sensor after reaction with tyrosinase in a sample solution; (d) comparing the surface enhanced Raman spectrum of the sample solution obtained in the step (c) with the fingerprint spectrum, and carrying out qualitative and quantitative analysis; and (e) enabling the sensor after detection to be recyclable by utilizing electrochemical regulation and control. The detection method has the advantages that the technical defects ofan existing detection method are overcome; the activity of the tyrosinase can be rapidly and sensitively detected; and the sensor is recyclable and is expected to realize automatic detection of the tyrosinase.