18 results about "Tyrosinase activity" patented technology

The application belongs to the technical field of biotechnology and particularly relates to a multifunctional bioactive peptide based on highland barley wine lees protein and a preparation method thereof, wherein the amino acid sequence of the bioactive peptide is shown as SEQ ID NO:1. The bioactive peptide has small molecular weight, is easy to absorb, can inhibit tyrosinase activity and efficiently scavenge free radicals, and has the advantages of non-toxicity, non-carcinogenicity, high hydrophilicity, long half-life and the like through bioinformatics analysis prediction, and can be applied to the development of drugs for treating skin pigmentation and oxidative stress related diseases or daily chemicals for whitening, anti-aging, antioxidant and the like, and has a wide application prospect.

This invention relates to a gentle whitening and anti-aging composition for sensitive skin, its preparation method, and its application, belonging to the field of cosmetic technology. The gentle whitening and anti-aging composition includes hibiscus flower extract, Tokyo cherry blossom leaf extract, redwood seed extract, and vitamin C peptides. The Tokyo cherry blossom leaf extract is obtained using a eutectic solvent (DES) combined with enzymatic extraction and resin purification. This invention constructs a multi-level synergistic whitening and anti-aging network using the above components. Efficacy testing verifies that the components in the gentle whitening composition provided by this invention can synergistically enhance the composition's ability to inhibit tyrosinase activity, scavenge DPPH free radicals, and promote type I collagen synthesis, thereby achieving skin brightening and whitening, firming and anti-wrinkle effects, improved elasticity, and reduced fine lines.

This invention relates to a whitening extract from geranium, its preparation method, and its application, belonging to the field of plant extract preparation and cosmetic technology. The extraction method includes: preparing a eutectic solvent by mixing choline chloride and citric acid in a molar ratio of 2:1 to 4:1, and adjusting the water content to 30-70%; mixing geranium raw material powder with the eutectic solvent at a material-liquid ratio of 1:5-15 (g / mL), and performing ultrasonic-assisted extraction; concentrating the extract under reduced pressure and then freeze-drying to obtain a geranium whitening extract with a total flavonoid content of 1-4 mg / g, a total polyphenol content of 3-6 mg / g, and anthocyanin content of 1-3 mg / g. This extract can be used to prepare cosmetics or topical skin preparations with whitening, brightening, antioxidant, or pigmentation-improving effects, as well as a tyrosinase activity inhibitor. This invention provides a green and efficient method for extracting the whitening active ingredients from geranium.

The application discloses a chitosan-glycine-copper chelate and a preparation method and application thereof in treating leukoplakia and vitiligo. In the preparation, chitosan is first placed in an acid aqueous phase system, and is fully dissolved and forms a uniform transparent or translucent solution under stirring; then glycine is added, and the chitosan and the glycine are fully mixed under stirring to build an organic ligand environment containing multiple coordination groups; subsequently, a copper source is introduced to perform a chemical reaction, and finally the chitosan-glycine-copper chelate is obtained. The chitosan-glycine-copper chelate shows a strong promoting activity completely different from all other substances (including simple mixtures of components thereof), the chelate can continuously and significantly activate tyrosinase activity, and promote the formation of melanin, so that the purpose of treating leukoplakia and vitiligo is achieved.

The present application relates to a ginsenoside composition for inhibiting tyrosinase activity, which comprises any two or any three of ginsenoside Rd, ginsenoside Re or ginsenoside Rf. Ginsenoside Rf can be used as a synergistic molecule of ginsenoside Rd and Re, and can significantly enhance the inhibitory ability of tyrosinase activity through synergistic effect, thereby reducing the synthesis and secretion of melanin.

The invention discloses a plant extract composition. The plant extract composition is prepared by mixing a paullinia cupana fruit extract and lycium barbarum polysaccharide according to a mass ratio of 1:(1-5). A preparation method of the paullinia cupana fruit extract comprises the following steps: (1) paullinia cupana fruits are dried and crushed, an ionic liquid ethanol solution is added, aftersealed infiltration is conducted for 1-2 h, a 30-50 V / V% ethanol solution is added, heating is conducted to achieve the reflux temperature, reflux extraction is conducted for 1-2 hours, filtering isconducted, filtrate is collected, and concentrating is conducted to obtain a crude extract; and (2) the crude extract is diluted with a proper amount of water, hydrochloric acid is added to adjust a pH value to 2.0-5.0, extracting with diethyl ether is conducted for 2-3 times, organic phases are combined, concentrating under reduced pressure is conducted, and drying is conducted. Compared with theprior art, a smaller amount of paullinia cupana fruits is adopted, so that the production cost is reduced, and the technical effect of better inhibiting tyrosinase activity is further achieved.

This invention provides a composition for suppressing melanin production and its use. [Solution] The composition contains platelets and / or growth factors isolated from blood and has the ability to suppress tyrosinase activity and alleviate inflammation. Furthermore, the composition of the present invention can be used in the manufacture of formulations for improving skin pigmentation or alleviating melasma.

The application discloses a collagen peptide, a collagen peptide salt, a polypeptide composition and application thereof, and belongs to the technical field of active peptide materials. The collagen peptide is a short peptide with an amino acid sequence as shown in SEQ ID NO:1 or a cyclic peptide containing the amino acid sequence as shown in SEQ ID NO:1. The collagen peptide can significantly inhibit tyrosinase activity and significantly inhibit melanin content in cells, thereby achieving a whitening effect.

This invention discloses a whitening and freckle-removing composition containing white tomato and *Gynostemma pentaphyllum*, its preparation method, and its application, belonging to the field of food technology. The whitening and freckle-removing composition consists of white tomato powder and *Gynostemma pentaphyllum* powder in a weight ratio of 1-3:2-7. The preparation method includes the following steps: (1) white tomato is pulped, separated, concentrated, and dried to obtain white tomato powder; (2) *Gynostemma pentaphyllum* is soaked in acidic water, and the solid and liquid are separated. The resulting clear liquid is concentrated and dried to obtain *Gynostemma pentaphyllum* powder; (3) white tomato powder and *Gynostemma pentaphyllum* powder are mixed. This invention utilizes the combination of white tomato and *Gynostemma pentaphyllum*, and through improvements in the preparation process of *Gynostemma pentaphyllum*, the two produce a synergistic effect in inhibiting tyrosinase activity and melanin production.

This invention relates to a plant composition with whitening effects and its application. The whitening plant composition includes extracts of *Scleroderma wilfordii*, *Myrothamnus flabellifolia*, and *Solanum nigrum* root. The whitening plant composition of this invention utilizes the combination of three plant extracts, resulting in a rational formulation where each active ingredient complements the others, synergistically enhancing their effects. This effectively inhibits tyrosinase activity and reduces melanin synthesis, exhibiting significant whitening efficacy. When applied to cosmetics, this plant composition results in stable products that also provide skin repair, reduce the irritation of whitening ingredients, and are suitable for various skin types, fully meeting consumers' demand for natural, gentle, and highly effective whitening products.

The application discloses a whitening composition containing controlled-release ascorbic acid and application thereof, and belongs to the technical field of cosmetic preparations. The composition comprises the following components in percentage by weight: Aframomum melegueta seed extract 0.1-5%, sodium mannose phosphate 0.1-5%, controlled-release ascorbic acid 10-20%, crocus sativus flower extract 0.1-5%, dipropylene glycol 15-30% and water in remainder. The application controls and releases ascorbic acid, significantly improves the stability and transdermal persistence of ascorbic acid in the formula, and realizes multi-target synergistic effect on the basis of compounding the controlled-release ascorbic acid with the Aframomum melegueta seed extract, the crocus sativus flower extract and the sodium mannose phosphate. Experiments prove that the composition can not only efficiently inhibit tyrosinase activity and melanin synthesis in B16F10 cells, but also has superiority in long-term stability and long-acting inhibition effect.

This invention provides a topical composition and its preparation method for reversing gray hair to black and repairing the scalp. The topical composition comprises the following raw materials in parts by weight: 10-30 parts of Polygonum multiflorum, 10-25 parts of Platycladus orientalis leaves, 8-20 parts of Artemisia argyi, 5-15 parts of ginger, 5-15 parts of Morus alba root bark, 5-12 parts of Leonurus japonicus, 5-12 parts of Lycium chinense root bark, 3-10 parts of Angelica dahurica, 5-15 parts of Morus alba leaves, 5-12 parts of Citrus reticulata peel, 3-10 parts of tea leaves, 3-10 parts of Auricularia auricula-judae, and 2-8 parts of Glycyrrhiza uralensis. The topical composition also contains a compound plant extract, which is obtained by alcohol extraction from Acer palmatum, Paederia scandens, and Areca catechu flowers in a mass ratio of (2.8-3.2):(0.9-1.1):(1.5-2.5). The composition of this invention can activate scalp tyrosinase activity and promote melanin production; improve scalp microcirculation and hair follicle nutrient supply; repair damaged hair and delay gray hair growth; achieve root-cause promotion of gray hair turning black, and also has scalp care function to restore black and shiny hair.

This invention discloses a small molecule peptide derived from *Hirudo medicinalis*, with the amino acid sequence Leu-Leu-Phe-Arg. The peptide was obtained by enzymatically hydrolyzing the waste protein residue after purifying hirudin from *Hirudo medicinalis*, combined with tyrosinase-functionalized magnetic bead affinity fishing technology and mass spectrometry identification. In vitro kinetics and molecular dynamics simulations confirmed that the peptide LLFR significantly prolongs the lag time of monophenolase reactions. It penetrates the catalytic pocket of tyrosinase through an "induced fit" mechanism, forming a stable dead-end complex with the enzyme and inducing the unfolding of the enzyme's tertiary structure. Cell experiments confirmed that LLFR significantly inhibits tyrosinase activity and melanin synthesis in melanoma cells without significant cytotoxicity. This peptide exhibits high safety and good water solubility, providing a new approach for the high-value utilization of industrial waste from *Hirudo medicinalis*, and is suitable for the industrial production of whitening cosmetics and functional products.

The present application provides a kind of multi-target synergistic whitening and freckle lightening plant composition and its preparation method and application, belong to medical, dental or cosmetic preparation field.The active ingredient is composed of Radix Glycyrrhizae, Radix Scutellariae, Coix Seed, Centella asiatica and mulberry root-bark extract according to specific proportion.The composition realizes signal pathway inhibition, tyrosinase activity blocking, melanin transport interference, keratin metabolism acceleration and skin barrier repair by constructing multi-target synergistic network.The present application overcomes the limitations of single whitening target, such as repeated color spots and slow effect, realizes whole-cycle and systematic whitening and freckle lightening effect under the premise of ensuring safety, and is suitable for cosmetic field.

The present application provides a liposome-encapsulated whitening composition, a preparation method and an application thereof, and belongs to the field of medical, dental or cosmetic preparations. The components of the present application are 4-butyl resorcinol, tranexamic acid, alpha-arbutin, coenzyme Q10, Ceylon cinnamon oil, sweep leaf Australian tea branch / leaf oil, hydrogenated soybean lecithin and a humectant. The present application is compounded with a variety of different whitening components, covering the three major pathways of "inhibiting tyrosinase activity, inhibiting melanin transfer and accelerating melanin metabolism" for melanin production; in line with the modern multi-target whitening trend. At the same time, the additional plant essential oil has the effect of promoting penetration and strengthening the inhibition of tyrosinase; at the same time, the liposome-encapsulated preparation solves the problem of mutual incompatibility between raw materials and increases the transdermal absorption rate and reduces the irritation. It can be added to a variety of different specific products, such as serums, creams, lotions, etc.

The present application relates to the technical field of skin care products, in particular to a composition for inhibiting tyrosinase activity, a whitening compound and a daily chemical product. The composition for inhibiting tyrosinase activity comprises 0.5-2.0 parts of hydroxytyrosol and 0.8-2.0 parts of resveratrol by weight. The composition can effectively inhibit the activity of tyrosinase and reduce the formation of melanin. Meanwhile, the compound provided by the present application can effectively reduce the occurrence of various skin diseases, has better biological activities such as whitening, antioxidant, anti-inflammatory, anti-aging, moisturizing and emollient, and has a good application prospect in the daily chemical market.

This invention relates to a method for preparing a composition of loaded active peptide vesicles and its application in skincare products. The composition utilizes an ultrasound-assisted aqueous two-phase extraction system to extract active peptides from *Bombyx mori*, and then encapsulates them with natural plant vesicles of sage and bergamot to form a complex. It can penetrate the epidermal layer of the skin, targeting fibroblasts and melanocytes, exhibiting excellent antioxidant properties and the ability to scavenge a large number of free radicals. It inhibits the activity of matrix metalloproteinases, thereby reducing collagen loss; inhibits tyrosinase activity and reduces melanin production; and inhibits the secretion of pro-inflammatory factors, thus maintaining a stable and balanced state of the skin. This composition can delay skin aging, brighten skin tone, and soothe inflammation.

The present application provides a kind of whitening, improve skin barrier composition and its preparation method and application, it is related to skin care product technical field.The composition raw material includes: plant extract 30-42 parts, Lotus nut extract 13-17 parts, Beauveria extract 12-14 parts, malt oligosaccharide glucoside 1.2-1.6 parts, raspberry ketone glucoside 2-3 parts, oligopeptide 4-6 parts, sucrose laurate 1-2 parts, humectant 2-4 parts, thickening agent 1-1.8 parts, water 280-340 parts.The present application can inhibit tyrosinase activity by the synergistic effect of specific plant extract, Lotus nut extract, Beauveria extract, malt oligosaccharide glucoside, raspberry ketone glucoside, oligopeptide and other raw materials, achieve good whitening effect, and make each active ingredient transdermal absorption, protect skin keratin layer lipid bilayer structure from being destroyed, maintain the water-retention capacity of skin, avoid water loss, thereby play the role of improving skin barrier.