207 results about "Tyrosinase activity" patented technology

The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.

The invention discloses a whitening composition containing PDRN and SOD (superoxide dismutase). The composition comprises a selaginella tamariscina extract, a mulberry root extract, a cystosella rubra extract and a nucleotide protein compound, and the nucleotide protein compound comprises the PDRN and the SOD. The composition obtained by scientific compatibility of the selaginella tamariscina extract, the mulberry root extract, the cystosella rubra extract and the nucleotide protein compound has a remarkable inhibition effect on tyrosinase activity, is safe and efficient, and can be widely applied to the field of cosmetics.

A group of polypeptides, which can be used as a tyrosinase inhibitor. The polypeptides have the effects of inhibiting tyrosinase activity, reducing melanin secretion, resisting oxidation, skin-whitening, spot-removing, etc. The polypeptides can be widely used in antioxidant drugs and in the preparation of skin-whitening cosmetics for resisting melanogenesis and lightening spots as a new raw material, and has good application scenarios.

The invention discloses a tyrosinase inhibitory peptide from kelp as well as a preparation method and application of the tyrosinase inhibitory peptide. The preparation method comprises the following steps: pretreating kelp, fermenting the kelp by using lactobacillus plantarum, carrying out centrifugation, ultrafiltration and component identification on the obtained fermentation liquor to obtain the composition of kelp polypeptide, screening the tyrosinase inhibitory peptide through molecular docking, and finally carrying out solid-phase synthesis to obtain the tyrosinase inhibitory peptide prepared by the invention. The lactobacillus plantarum fermentation technology is simple and feasible, the production cost is low, equipment is easy to obtain, active ingredients in kelp can be released to the maximum extent, and the prepared polypeptide has the effect of inhibiting the activity of tyrosinase and can be used for developing a tyrosinase inhibitor.

The invention discloses a polypeptide with a whitening effect and a preparation method and application thereof.The polypeptide is obtained from hydrolyzed protein of babylonia areolata, the amino acid sequence of the polypeptide is cysteine-arginine-proline-threonine-cysteine-serine-arginine-leucine-alanine-cysteine, and the amino acid sequence of the polypeptide is cysteine-arginine-proline-threonine-cysteine-serine-arginine-leucine-alanine. The compound can obviously inhibit the generation of melanin in cells and the activity of tyrosinase in vitro, has the characteristics of low toxicity, low irritation, good stability, safety and high efficiency, and can be applied to whitening cosmetics.

The invention discloses a whitening active peptide with an effect of inhibiting melanin formation as well as a preparation method and application of the whitening active peptide. The amino acid sequence of the whitening active peptide is as follows: YYYDPWAGK. Whitening activity verification is carried out on the peptide, the relative tyrosinase activity in cells can be remarkably reduced to 164.26 + / -3.03% from 245.52 + / -1.55% by the peptide with the concentration of 200 mu g / mL, and meanwhile, the relative melanin content can be reduced to 130.05 + / -1.23% from 206.65 + / -4.33%. In addition, the peptide not only can significantly reduce the relative tyrosinase activity of zebrafish from 100 + / -0.36% to 60.42 + / -0.73%, but also can reduce the relative melanin content of zebrafish from 100 + / -2.01% to 63.74 + / -2.05%, and lays an important theoretical foundation for the research and application of zebrafish in the fields of functional food, health care products, cosmetics and medicines.

The application discloses a whitening and freckle-removing composition, conditioner, medicine and cosmetic. The whitening and freckle-removing composition comprises broccoli, ganoderma lucidum and inula japonica in the composition; the broccoli comprises broccoli raw materials and / or broccoli extract, the ganoderma lucidum comprises ganoderma lucidum raw materials and / or ganoderma lucidum extract, and the inula japonica comprises inula japonica raw materials and / or inula japonica extract. The application has significant synergistic activity by scientific compounding of the broccoli and the inula japonica, has significant inhibitory effect on tyrosinase activity, can inhibit the expression and function of the rate-limiting enzyme TYR in the melanin synthesis process, has significant whitening and freckle-removing activity, is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The invention relates to a composition with whitening and skin tendering effects, essence lotion and a preparation method and application of the essence lotion, in the composition with the whitening and skin tendering effects, the weight ratio of achillea millefolium extract to hydroxyethyl piperazine ethanesulfonic acid to ormosia henryi bark extract is (0.1-1): (0.5-8): (0.05-0.5). The essence lotion is prepared from the following components in percentage by weight: 0.75 to 6 percent of composition with skin whitening and tendering effects, 3 to 8 percent of emollient, 0.5 to 2 percent of emulsifier, 0.01 to 1 percent of filler, 0.5 to 2 percent of thickener, 0.8 to 1.5 percent of preservative, 0.02 to 0.2 percent of daily essence, 0.1 to 10 percent of skin conditioner, 0.1 to 10 percent of humectant and the balance of deionized water. The main components have a synergistic effect, can effectively regulate and control the information network effect of endothelin and reduce the differentiation, proliferation and the like of melanocytes in the first step of melanin generation by inhibiting the activity of tyrosinase, can promote cell autophagy and effectively metabolize melanin and lipofuscin generated in the skin, and achieves the effects of whitening, whitening, whitening and the like. The skin tendering effect is realized.

The invention discloses a whitening and freckle-removing composition, a conditioner, a medicine and a cosmetic. The whitening and freckle-removing composition is prepared from emblic leafflower fruit, passion fruit and Chinese ladiestresses root and leaf A; the phyllanthus emblica comprises a phyllanthus emblica raw material and / or a phyllanthus emblica extract, and the passion fruit comprises a passion fruit raw material and / or a passion fruit extract. Through scientific compatibility of the Chinese ladiestresses root-ginseng A and the second active component comprising one or more of the emblic leafflower fruit, the passion fruit and the salvia officinalis, the Chinese ladiestresses root-ginseng A has a remarkable inhibition effect on tyrosinase activity, can inhibit expression and functions of rate-limiting enzyme TYR in the melanin synthesis process, has a remarkable whitening and freckle-removing active effect, and has a remarkable whitening and freckle-removing effect. The composition is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The invention belongs to the technical field of daily chemical industry, and discloses a carbon quantum dot with a whitening function and a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a carbon-containing whitening component, a nitrogen source and water, carrying out hydrothermal reaction, cooling, filtering and dialyzing to obtain the carbon quantum dots with the whitening function. The carbon quantum dots are subjected to high-temperature treatment in the synthesis process, the obtained product has thermal stability and light stability, the problem that traditional whitening components are unstable is solved, and the application scene of whitening cosmetics under the illumination condition is expanded. The quantum dot not only retains the whitening disclosure of a carbon-containing whitening component, but also has the function of absorbing ultraviolet rays, can reduce the generation of skin melanin from two pathways of physically shielding ultraviolet rays and chemically inhibiting tyrosinase activity, and has dual effects; compared with common carbon quantum dots and whitening components which only have a single whitening pathway, melanin generation is more efficiently inhibited. Therefore, the carbon quantum dots can be used for preparing whitening skin care products.

The invention discloses a tyrosinase inhibitor, a preparation method thereof and application of the tyrosinase inhibitor in preparation of a product for inhibiting tyrosinase activity. Based on the protein structure of the TYR, the TYR inhibitor which is novel in structure and higher in curative effect and selectivity is found by adopting reasonable drug design and structural modification strategies.

The application discloses application of a complex containing Dendrobium officinale leaf extract in preparation of a whitening and freckle-removing preparation, and the complex comprises the Dendrobium officinale leaf extract, a Rhizoma Polygonati extract and beta arbutin. After the components of the complex are mixed in a specific ratio and fully combined and synergized, the ability of inhibiting tyrosinase activity can be significantly improved by 300%, 175% and 87% respectively compared with the Dendrobium officinale leaf, the Rhizoma Polygonati and the beta arbutin, the melanin generation in zebra fish within 72 hours can be effectively inhibited, the melanin content can be obviously reduced, the melanin inhibition rate is increased by 394%, 532%, 24.14% and 37.39% respectively compared with the Dendrobium officinale leaf group, the Rhizoma Polygonati group, the beta arbutin group and the Dendrobium officinale leaf-Rhizoma Polygonati group, the Dendrobium officinale leaf-Rhizoma Polygonati complex can obviously improve the melanin inhibition rate in zebra fish, the combination of the two and the beta arbutin can make the melanin inhibition rate reach 98.70%, and the whitening and freckle-removing effect of skin can be greatly enhanced.

The invention discloses a nanometer emulsion based on a citrus extract and a preparation method of the nanometer emulsion. The nano-emulsion takes citrus pectin as a main chain, gallic acid, ascorbic acid-phosphate and oleic acid are sequentially grafted, the oleic acid is lipophilic, the rest is hydrophilic, and the amphiphilic pectin can form the whitening nano-emulsion which can be stably stored for a long time. The natural citrus extract is used for replacing traditional synthetic components, has the anti-oxidation, whitening and anti-inflammatory effects, can reduce the skin sensitivity risk, and conforms to the green beauty makeup trend; meanwhile, the oil phase is anchored by a hydrophobic block, steric hindrance is formed by a hydrophilic block, the stability of the emulsion is remarkably improved, and the shelf life is prolonged to 24 months; phenolic acid and ascorbic acid contained in the traditional Chinese medicine composition can effectively scavenge free radicals, inhibit tyrosinase activity, reduce melanogenesis and reduce release of a proinflammatory factor IL-6; the composition is low in cost, environment-friendly, suitable for the fields of cosmetics and medicines and wide in market prospect.

The present invention relates to a plant composition with whitening and freckle-removing effects and its application, belonging to the technical field of compositions. The plant composition comprises the following raw materials: sea fennel extract, osmanthus extract, barley extract, smooth fruit elm bark extract, black sesame extract, thyme extract, spinach extract and a solvent. The present invention combines sea fennel extract, osmanthus extract and barley extract, starting from a triple whitening mechanism of inhibiting tyrosinase activity, inhibiting melanin formation caused by α-MSH, and inhibiting melanocyte activity, to synergistically improve the whitening effect of the composition. In addition, three freckle-removing plant extracts are added to the formula of the present invention. The smooth fruit elm bark extract is used to reduce freckles, the black sesame extract is used to enhance the cell melanin metabolism ability, and the thyme extract is used to stimulate the growth of new cells. The three make the composition prepared by the present invention achieve a synergistic effect of freckle-removing from three perspectives.

The invention belongs to the technical field of chemical extraction, and particularly relates to a method for one-step extraction of litchi pulp polysaccharide and polyphenol based on a deep-eutectic solvent. A choline chloride-lactic acid system is used for extracting and preparing for a certain period of time through a deep-eutectic solvent, a supernatant obtained through centrifugation is lychee pulp polyphenol, a lychee pulp polysaccharide extracting solution can be obtained by washing precipitates with water for three times, and one-step efficient extraction of polyphenol and polysaccharide is achieved. The LPPP extracted by DES has strong antioxidant ability and tyrosinase activity inhibition ability, which is speculated that the LPPP can effectively inhibit the synthesis of melanin. Therefore, the litchi pulp polyphenol has high biological activity and can be used as a functional additive of food or cosmetics.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

The invention relates to the technical field of whitening and freckle removing, in particular to light-sensitive whitening and freckle removing essence and a preparation method and application thereof.The light-sensitive whitening and freckle removing essence is prepared from, by mass, 72.07% of water, 6% of butanediol, 3%-5% of X-resveratrol, 3% of tranexamic acid, 2.5% of nicotinamide, 2% of a red removing factor, 1% of panthenol, 1% of an anti-inflammatory agent, 0.5% of an anti-allergy agent, 0.5% of carnosine, 0.5% of p-hydroxyacetophenone, 1% of 1, 2-propylene glycol and the balance 1, 2-propylene glycol. The mask comprises the following components in percentage by weight: 0.5% of 1, 2-hexanediol, 0.2% of carbomer, 0.18% of arginine, 0.05% of sodium hyaluronate, 0.1%-1% of a light stabilizer and 5% of bis-ethylhexyloxyphenol sulfonate. According to the invention, the resveratrol is esterified by methyl propiolate, so that the molecular structure of the resveratrol is optimized, the performance of the resveratrol in the aspect of light stability is enhanced, and the degradation under the illumination condition is reduced; the esterified resveratrol can effectively inhibit tyrosinase activity, regulate melanocyte signal transduction and realize whitening and spot fading effects.

The invention provides a lactobacillus plantarum 18 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31277. The lactobacillus plantarum 18 has the advantages that the lactobacillus plantarum 18 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 18 can inhibit growth of propionibacterium acnes, inhibit expression of virulence factors (biological membranes, lipase and the like) of the propionibacterium acnes, relieve inflammation, inhibit autophagy of cells, scavenge free radicals and inhibit activity of tyrosinase, so that the plant lactobacillus 18 has the effects of resisting acnes, dermatitis and oxidation and whitening skin. The plant lactobacillus 18 or the metabolite of the plant lactobacillus 18 can be used for preparing products with the effects or effects.

This invention relates to the field of edible fungi technology, and discloses a new strain of Auricularia heimuer and its applications. The Auricularia heimuer strain JH-Y6 of this invention was deposited at the China Center for Type Culture Collection (CCTCC) on November 20, 2023, with accession number CCTCC No: M 20232280. Auricularia heimuer JH-Y6 possesses high tyrosinase activity, high melanin production, and excellent agronomic traits, and can be used in the screening and breeding of Auricularia heimu, the extraction process of melanin from Auricularia heimu, and its cultivation. It can also be used in the deep processing of food or health products.

The invention discloses an anti-oxidation, whitening and brightening composition as well as a preparation method and application thereof. The anti-oxidation, whitening and brightening composition is prepared from the following components in parts by weight: 2 to 6 parts of nicotinamide, 0.5 to 5 parts of tranexamic acid, 1 to 5 parts of nonapeptide-1 solution, 0.5 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 2 parts of pink red plum compound essence, 0.01 to 0.5 part of hydroxyethyl piperazine ethanesulfonic acid, 0.01 to 0.5 part of glabridin and 0.0001 to 0.2 part of glutathione. According to the invention, the composition prepared from nicotinamide, tranexamic acid, nonapeptide-1, tetrahydromethylpyrimidine carboxylic acid, pink-red plum composite essence, hydroxyethyl piperazine ethanesulfonic acid, glabridin and glutathione is prepared into the essence, and the clearance rate of the essence to ABTS free radicals reaches 58% or above, which shows that the essence has very good antioxidant ability; the cell tyrosinase activity inhibition rate of the composition under the concentration of 1.2% (v / v) reaches 51% or above, which shows that the composition has very good whitening and brightening effects.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention belongs to the technical field of preparation and application of natural products, and particularly relates to a perilla frutescens extract product for activating mitochondrial autophagy and resisting aging and application of the perilla frutescens extract product. Perillaldehyde is obtained by purifying perilla leaves, and the purity reaches 99%. The compound has DPPH free radical scavenging capacity and tyrosinase activity inhibition capacity, and the DPPH free radical scavenging capacity is equivalent to that of vitamin E; the service life of the high-concentration perillaldehyde group is prolonged by 8%; the service life of caenorhabditis elegans under the environments of thermal stimulation, ultraviolet stimulation and oxide stimulation is remarkably prolonged, and the adversity service life prolonging rate of a high-concentration perillaldehyde group is at least 20%; sOD (superoxide dismutase) and CAT (catalase) activity of caenorhabditis elegans is remarkably improved, H2O2 accumulation is reduced, and anti-stress capability is improved; the caenorhabditis elegans mitochondrial autophagy is promoted, the mitochondrial autophagy pathway related genes are adjusted, the mitochondrial function is improved, the NAD < + > / NADH ratio is increased, the body metabolism is maintained, and the aging is delayed; the perillaldehyde capable of promoting the mitochondrial autophagy pathway can be applied to development of anti-aging drugs, health care products, food, cosmetics and other products.

The present invention addresses the problem of providing a skin whitening and beautifying composition having an excellent tyrosinase activity inhibitory effect. The present inventors have completed the present invention discovering that an excellent tyrosinase activity inhibitory effect can be obtained by using glutathione (GSH), which is a cysteine-containing peptide, in combination with gamma-Gly-Cys (gamma-GC), Cys-Gly (CG). In particular, it is preferable that the composition ratio (weight ratio) of GSH, gamma-GC, and CG is 1: 0.01-1: 0.005-0.50.

The invention relates to the technical field of biological detection, and discloses a tyrosinase activity detection method based on in-situ fluorescence generation and application, and the method comprises the following steps: mixing L-tyrosine with 3-hydroxyphenylboronic acid; adding a biological enzyme sample solution, incubating at a constant temperature, and accumulating L-dopa by enzyme catalysis; then adding an alkaline adjusting solution to construct an alkaline environment, inducing the 3-hydroxyphenylboronic acid and L-dopa to generate a specific boric acid esterification reaction, and generating a fluorescent borate compound in situ; and finally, adding a constant-volume stop solution to maintain an alkaline environment and complement the volume, and detecting the fluorescence intensity. According to the method, a step-by-step pH regulation strategy is adopted, the enzyme activity is guaranteed under the neutral condition, then color development is promoted under the alkaline condition, and the problem that dynamic conditions of the two are incompatible is solved. The method has the advantages of low background interference, high sensitivity, good reproducibility and the like by utilizing specific recognition of a phenylboronic acid group on a catechol structure, and can be applied to food browning analysis, whitening drug screening and disease diagnosis.

The application discloses a high-efficiency absorption whitening and freckle-removing skin care product and a preparation process thereof, and belongs to the technical field of cosmetics. The high-efficiency absorption whitening and freckle-removing skin care product contains the following components in terms of weight parts: 0.1-5 parts of a whitening compound (I), 5-10 parts of cocoyl glucoside, 0.5-1 part of menthol, 5-10 parts of phospholipid, 0.5-1 part of EDTA, 0.1-1 part of hyaluronic acid and 150-200 parts of deionized water. The whitening and freckle-removing skin care product has good antioxidant and tyrosinase enzyme activity inhibiting effects, and can realize the effects of whitening and freckle removal.

The invention discloses jellyfish polypeptide with tyrosinase inhibitory activity and application of the jellyfish polypeptide, and belongs to the technical field of biology. A jellyfish crude polypeptide is prepared, a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and optimized from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit the activity of tyrosinase in vitro, can inhibit melanin secretion of B16-F10 mouse melanoma cells, and can inhibit the activity of tyrosinase in the cells. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and is applied to the fields of cosmetics, medical treatment and the like. Experiments prove that the jellyfish polypeptide provided by the invention has no cytotoxicity, is derived from ocean, and has no disease risks and religious barriers of terrestrial animals.

The application belongs to the technical field of biotechnology and particularly relates to a multifunctional bioactive peptide based on highland barley wine lees protein and a preparation method thereof, wherein the amino acid sequence of the bioactive peptide is shown as SEQ ID NO:1. The bioactive peptide has small molecular weight, is easy to absorb, can inhibit tyrosinase activity and efficiently scavenge free radicals, and has the advantages of non-toxicity, non-carcinogenicity, high hydrophilicity, long half-life and the like through bioinformatics analysis prediction, and can be applied to the development of drugs for treating skin pigmentation and oxidative stress related diseases or daily chemicals for whitening, anti-aging, antioxidant and the like, and has a wide application prospect.

This invention relates to a gentle whitening and anti-aging composition for sensitive skin, its preparation method, and its application, belonging to the field of cosmetic technology. The gentle whitening and anti-aging composition includes hibiscus flower extract, Tokyo cherry blossom leaf extract, redwood seed extract, and vitamin C peptides. The Tokyo cherry blossom leaf extract is obtained using a eutectic solvent (DES) combined with enzymatic extraction and resin purification. This invention constructs a multi-level synergistic whitening and anti-aging network using the above components. Efficacy testing verifies that the components in the gentle whitening composition provided by this invention can synergistically enhance the composition's ability to inhibit tyrosinase activity, scavenge DPPH free radicals, and promote type I collagen synthesis, thereby achieving skin brightening and whitening, firming and anti-wrinkle effects, improved elasticity, and reduced fine lines.

The application discloses a whitening and freckle-removing composition containing white tomato and chases yellow grass, a preparation method and application thereof, and belongs to the technical field of food, wherein the whitening and freckle-removing composition is composed of white tomato powder and chases yellow grass powder with a weight ratio of 1-3:2-7. The preparation method comprises the following steps: (1) beating, separating, concentrating and drying white tomatoes to obtain white tomato powder; (2) soaking chases yellow grass in acidic water, performing solid-liquid separation, concentrating and drying the obtained clear liquid to obtain chases yellow grass powder; and (3) mixing the white tomato powder and the chases yellow grass powder. The application utilizes the combination of white tomatoes and chases yellow grass, and improves the preparation process of chases yellow grass, so that the two components produce a synergistic effect in inhibiting tyrosinase activity and inhibiting melanin production.

This invention relates to a whitening extract from geranium, its preparation method, and its application, belonging to the field of plant extract preparation and cosmetic technology. The extraction method includes: preparing a eutectic solvent by mixing choline chloride and citric acid in a molar ratio of 2:1 to 4:1, and adjusting the water content to 30-70%; mixing geranium raw material powder with the eutectic solvent at a material-liquid ratio of 1:5-15 (g / mL), and performing ultrasonic-assisted extraction; concentrating the extract under reduced pressure and then freeze-drying to obtain a geranium whitening extract with a total flavonoid content of 1-4 mg / g, a total polyphenol content of 3-6 mg / g, and anthocyanin content of 1-3 mg / g. This extract can be used to prepare cosmetics or topical skin preparations with whitening, brightening, antioxidant, or pigmentation-improving effects, as well as a tyrosinase activity inhibitor. This invention provides a green and efficient method for extracting the whitening active ingredients from geranium.