127 results about "Tyrosinase activity" patented technology

The invention discloses a whitening composition containing PDRN and SOD (superoxide dismutase). The composition comprises a selaginella tamariscina extract, a mulberry root extract, a cystosella rubra extract and a nucleotide protein compound, and the nucleotide protein compound comprises the PDRN and the SOD. The composition obtained by scientific compatibility of the selaginella tamariscina extract, the mulberry root extract, the cystosella rubra extract and the nucleotide protein compound has a remarkable inhibition effect on tyrosinase activity, is safe and efficient, and can be widely applied to the field of cosmetics.

The application discloses a whitening and freckle-removing composition, conditioner, medicine and cosmetic. The whitening and freckle-removing composition comprises broccoli, ganoderma lucidum and inula japonica in the composition; the broccoli comprises broccoli raw materials and / or broccoli extract, the ganoderma lucidum comprises ganoderma lucidum raw materials and / or ganoderma lucidum extract, and the inula japonica comprises inula japonica raw materials and / or inula japonica extract. The application has significant synergistic activity by scientific compounding of the broccoli and the inula japonica, has significant inhibitory effect on tyrosinase activity, can inhibit the expression and function of the rate-limiting enzyme TYR in the melanin synthesis process, has significant whitening and freckle-removing activity, is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The application discloses application of a complex containing Dendrobium officinale leaf extract in preparation of a whitening and freckle-removing preparation, and the complex comprises the Dendrobium officinale leaf extract, a Rhizoma Polygonati extract and beta arbutin. After the components of the complex are mixed in a specific ratio and fully combined and synergized, the ability of inhibiting tyrosinase activity can be significantly improved by 300%, 175% and 87% respectively compared with the Dendrobium officinale leaf, the Rhizoma Polygonati and the beta arbutin, the melanin generation in zebra fish within 72 hours can be effectively inhibited, the melanin content can be obviously reduced, the melanin inhibition rate is increased by 394%, 532%, 24.14% and 37.39% respectively compared with the Dendrobium officinale leaf group, the Rhizoma Polygonati group, the beta arbutin group and the Dendrobium officinale leaf-Rhizoma Polygonati group, the Dendrobium officinale leaf-Rhizoma Polygonati complex can obviously improve the melanin inhibition rate in zebra fish, the combination of the two and the beta arbutin can make the melanin inhibition rate reach 98.70%, and the whitening and freckle-removing effect of skin can be greatly enhanced.

This invention relates to the field of edible fungi technology, and discloses a new strain of Auricularia heimuer and its applications. The Auricularia heimuer strain JH-Y6 of this invention was deposited at the China Center for Type Culture Collection (CCTCC) on November 20, 2023, with accession number CCTCC No: M 20232280. Auricularia heimuer JH-Y6 possesses high tyrosinase activity, high melanin production, and excellent agronomic traits, and can be used in the screening and breeding of Auricularia heimu, the extraction process of melanin from Auricularia heimu, and its cultivation. It can also be used in the deep processing of food or health products.

The invention discloses an anti-oxidation, whitening and brightening composition as well as a preparation method and application thereof. The anti-oxidation, whitening and brightening composition is prepared from the following components in parts by weight: 2 to 6 parts of nicotinamide, 0.5 to 5 parts of tranexamic acid, 1 to 5 parts of nonapeptide-1 solution, 0.5 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 2 parts of pink red plum compound essence, 0.01 to 0.5 part of hydroxyethyl piperazine ethanesulfonic acid, 0.01 to 0.5 part of glabridin and 0.0001 to 0.2 part of glutathione. According to the invention, the composition prepared from nicotinamide, tranexamic acid, nonapeptide-1, tetrahydromethylpyrimidine carboxylic acid, pink-red plum composite essence, hydroxyethyl piperazine ethanesulfonic acid, glabridin and glutathione is prepared into the essence, and the clearance rate of the essence to ABTS free radicals reaches 58% or above, which shows that the essence has very good antioxidant ability; the cell tyrosinase activity inhibition rate of the composition under the concentration of 1.2% (v / v) reaches 51% or above, which shows that the composition has very good whitening and brightening effects.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention relates to the technical field of biological detection, and discloses a tyrosinase activity detection method based on in-situ fluorescence generation and application, and the method comprises the following steps: mixing L-tyrosine with 3-hydroxyphenylboronic acid; adding a biological enzyme sample solution, incubating at a constant temperature, and accumulating L-dopa by enzyme catalysis; then adding an alkaline adjusting solution to construct an alkaline environment, inducing the 3-hydroxyphenylboronic acid and L-dopa to generate a specific boric acid esterification reaction, and generating a fluorescent borate compound in situ; and finally, adding a constant-volume stop solution to maintain an alkaline environment and complement the volume, and detecting the fluorescence intensity. According to the method, a step-by-step pH regulation strategy is adopted, the enzyme activity is guaranteed under the neutral condition, then color development is promoted under the alkaline condition, and the problem that dynamic conditions of the two are incompatible is solved. The method has the advantages of low background interference, high sensitivity, good reproducibility and the like by utilizing specific recognition of a phenylboronic acid group on a catechol structure, and can be applied to food browning analysis, whitening drug screening and disease diagnosis.

The application discloses a high-efficiency absorption whitening and freckle-removing skin care product and a preparation process thereof, and belongs to the technical field of cosmetics. The high-efficiency absorption whitening and freckle-removing skin care product contains the following components in terms of weight parts: 0.1-5 parts of a whitening compound (I), 5-10 parts of cocoyl glucoside, 0.5-1 part of menthol, 5-10 parts of phospholipid, 0.5-1 part of EDTA, 0.1-1 part of hyaluronic acid and 150-200 parts of deionized water. The whitening and freckle-removing skin care product has good antioxidant and tyrosinase enzyme activity inhibiting effects, and can realize the effects of whitening and freckle removal.

The invention discloses jellyfish polypeptide with tyrosinase inhibitory activity and application of the jellyfish polypeptide, and belongs to the technical field of biology. A jellyfish crude polypeptide is prepared, a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and optimized from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit the activity of tyrosinase in vitro, can inhibit melanin secretion of B16-F10 mouse melanoma cells, and can inhibit the activity of tyrosinase in the cells. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and is applied to the fields of cosmetics, medical treatment and the like. Experiments prove that the jellyfish polypeptide provided by the invention has no cytotoxicity, is derived from ocean, and has no disease risks and religious barriers of terrestrial animals.

The application belongs to the technical field of biotechnology and particularly relates to a multifunctional bioactive peptide based on highland barley wine lees protein and a preparation method thereof, wherein the amino acid sequence of the bioactive peptide is shown as SEQ ID NO:1. The bioactive peptide has small molecular weight, is easy to absorb, can inhibit tyrosinase activity and efficiently scavenge free radicals, and has the advantages of non-toxicity, non-carcinogenicity, high hydrophilicity, long half-life and the like through bioinformatics analysis prediction, and can be applied to the development of drugs for treating skin pigmentation and oxidative stress related diseases or daily chemicals for whitening, anti-aging, antioxidant and the like, and has a wide application prospect.

This invention relates to a gentle whitening and anti-aging composition for sensitive skin, its preparation method, and its application, belonging to the field of cosmetic technology. The gentle whitening and anti-aging composition includes hibiscus flower extract, Tokyo cherry blossom leaf extract, redwood seed extract, and vitamin C peptides. The Tokyo cherry blossom leaf extract is obtained using a eutectic solvent (DES) combined with enzymatic extraction and resin purification. This invention constructs a multi-level synergistic whitening and anti-aging network using the above components. Efficacy testing verifies that the components in the gentle whitening composition provided by this invention can synergistically enhance the composition's ability to inhibit tyrosinase activity, scavenge DPPH free radicals, and promote type I collagen synthesis, thereby achieving skin brightening and whitening, firming and anti-wrinkle effects, improved elasticity, and reduced fine lines.

The application discloses a whitening and freckle-removing composition containing white tomato and chases yellow grass, a preparation method and application thereof, and belongs to the technical field of food, wherein the whitening and freckle-removing composition is composed of white tomato powder and chases yellow grass powder with a weight ratio of 1-3:2-7. The preparation method comprises the following steps: (1) beating, separating, concentrating and drying white tomatoes to obtain white tomato powder; (2) soaking chases yellow grass in acidic water, performing solid-liquid separation, concentrating and drying the obtained clear liquid to obtain chases yellow grass powder; and (3) mixing the white tomato powder and the chases yellow grass powder. The application utilizes the combination of white tomatoes and chases yellow grass, and improves the preparation process of chases yellow grass, so that the two components produce a synergistic effect in inhibiting tyrosinase activity and inhibiting melanin production.

This invention relates to a whitening extract from geranium, its preparation method, and its application, belonging to the field of plant extract preparation and cosmetic technology. The extraction method includes: preparing a eutectic solvent by mixing choline chloride and citric acid in a molar ratio of 2:1 to 4:1, and adjusting the water content to 30-70%; mixing geranium raw material powder with the eutectic solvent at a material-liquid ratio of 1:5-15 (g / mL), and performing ultrasonic-assisted extraction; concentrating the extract under reduced pressure and then freeze-drying to obtain a geranium whitening extract with a total flavonoid content of 1-4 mg / g, a total polyphenol content of 3-6 mg / g, and anthocyanin content of 1-3 mg / g. This extract can be used to prepare cosmetics or topical skin preparations with whitening, brightening, antioxidant, or pigmentation-improving effects, as well as a tyrosinase activity inhibitor. This invention provides a green and efficient method for extracting the whitening active ingredients from geranium.

The present application relates to a kind of anti-ultraviolet damage composition and its application in sunscreen product, the anti-ultraviolet damage composition includes the following raw materials by weight parts: 5-8 parts of daphne giraldii leaf extract, 3-7 parts of green algae extract, 1-4 parts of dogwood extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado butter and 5-8 parts of tea seed oil, each raw material has synergistic effect, by neutralizing the free radical induced by ultraviolet, reduce oxidative stress, protect skin cells from damage;Absorb ultraviolet, reduce the penetration of ultraviolet through skin, thereby reducing the direct damage of ultraviolet to skin;Regulate tyrosinase activity or block the melanin synthesis pathway, reduce the accumulation of melanin, help to prevent and improve the efficacy of anti-ultraviolet damage such as pigmentation, dull and other ways.

The application discloses a kind of nanodroplet based on citrus extract and preparation method thereof.The nanodroplet is grafted with gallic acid, ascorbic acid-phosphate and oleic acid in turn with citrus pectin as main chain, and oleic acid is lipophilic, and the rest is hydrophilic, and amphiphilic pectin can form long-term stable storage whitening nanodroplet.The application replaces traditional synthetic ingredients with natural citrus extract, has antioxidant, whitening and anti-inflammatory effects, can reduce the risk of skin sensitivity, and meets the trend of green beauty;At the same time, the hydrophobic block anchors the oil phase, and the hydrophilic segment forms steric hindrance, which significantly improves the stability of the emulsion and prolongs the shelf life to 24 months;The phenolic acid and ascorbic acid contained therein can effectively scavenge free radicals, inhibit tyrosinase activity, reduce melanin production and reduce the release of pro-inflammatory factor IL-6;It is low in cost and environmentally friendly, suitable for cosmetics and pharmaceutical field, and has broad market prospect.

A composition for promoting tyrosinase activity, which includes a peptide consisting of the amino acid sequence of General Formula 1 below: K—Y—R1-R2-R3-R4-R5-R6-R7-R8 (General Formula 1) is disclosed. In General Formula 1, R1 is arginine (R), lysine (K), or glutamine (Q); R2 is arginine (R) or glutamine (Q); R3, R4, and R5 are each arginine (R) or lysine (K); R6 is asparagine (N) or serine (S); and R7 and R8 are each lysine (K) or tyrosine (Y). The composition may promote melanogenesis in melanocytes to prevent, improve or treat hypomelanosis.

This invention discloses a skin-whitening composition that reduces aging melanocytes and its application, relating to the cosmetics field. The invention provides a composition that, by reducing aging melanocytes and decreasing SASP secretion, reduces melanin production and inhibits tyrosinase activity while maintaining healthy melanocytes, thus maintaining skin homeostasis while whitening the skin. This composition can be widely used in various skincare products to effectively achieve long-lasting whitening effects.

The application discloses a chitosan-glycine-copper chelate and a preparation method and application thereof in treating leukoplakia and vitiligo. In the preparation, chitosan is first placed in an acid aqueous phase system, and is fully dissolved and forms a uniform transparent or translucent solution under stirring; then glycine is added, and the chitosan and the glycine are fully mixed under stirring to build an organic ligand environment containing multiple coordination groups; subsequently, a copper source is introduced to perform a chemical reaction, and finally the chitosan-glycine-copper chelate is obtained. The chitosan-glycine-copper chelate shows a strong promoting activity completely different from all other substances (including simple mixtures of components thereof), the chelate can continuously and significantly activate tyrosinase activity, and promote the formation of melanin, so that the purpose of treating leukoplakia and vitiligo is achieved.

The present application relates to a ginsenoside composition for inhibiting tyrosinase activity, which comprises any two or any three of ginsenoside Rd, ginsenoside Re or ginsenoside Rf. Ginsenoside Rf can be used as a synergistic molecule of ginsenoside Rd and Re, and can significantly enhance the inhibitory ability of tyrosinase activity through synergistic effect, thereby reducing the synthesis and secretion of melanin.

The invention discloses a plant extract composition. The plant extract composition is prepared by mixing a paullinia cupana fruit extract and lycium barbarum polysaccharide according to a mass ratio of 1:(1-5). A preparation method of the paullinia cupana fruit extract comprises the following steps: (1) paullinia cupana fruits are dried and crushed, an ionic liquid ethanol solution is added, aftersealed infiltration is conducted for 1-2 h, a 30-50 V / V% ethanol solution is added, heating is conducted to achieve the reflux temperature, reflux extraction is conducted for 1-2 hours, filtering isconducted, filtrate is collected, and concentrating is conducted to obtain a crude extract; and (2) the crude extract is diluted with a proper amount of water, hydrochloric acid is added to adjust a pH value to 2.0-5.0, extracting with diethyl ether is conducted for 2-3 times, organic phases are combined, concentrating under reduced pressure is conducted, and drying is conducted. Compared with theprior art, a smaller amount of paullinia cupana fruits is adopted, so that the production cost is reduced, and the technical effect of better inhibiting tyrosinase activity is further achieved.

A cosmetic composition is provided, which comprises a carrier and a Patchouli stem extract, in particular an extract from exhausted Patchouli stems. This composition provides anti-oxidant, anti-elastase, anti-hyaluronase, and anti-tyrosinase activities.

The invention relates to the technical field of drug synthesis, in particular to a 1-(3-chloro-4-fluorobenzyl) piperazine chromone derivative, a preparation method and application of the 1-(3-chloro-4-fluorobenzyl) piperazine chromone derivative as a tyrosinase inhibitor, and the 1-(3-chloro-4-fluorobenzyl) piperazine chromone derivative has a structural formula shown in a formula (I). Wherein R is one or more substituent groups; r is independently selected from one or more of hydrogen, hydroxyl, halogen, alkyl and alkoxy and has a relatively strong tyrosinase inhibition effect, and compared with positive control kojic acid, the tyrosinase inhibition effect is maximally improved by 168 times; the compound has a reversible and mixed inhibition effect on tyrosinase; the influence on melanogenesis and tyrosinase activity of the B16F10 cells in vitro is carried out at the concentration of 3-90 mu M, and no cytotoxicity is caused to the B16F10 cells; under the same test concentration, the compound W2 has higher melanin inhibition activity than kojic acid, has excellent druggability, and has wide application prospects.

Disclosed is a composition having whitening efficacy. The composition comprises artemisinic acid and further comprises nicotinamide and / or glutathione. The compounding of artemisinic acid and nicotinamide and / or glutathione inhibits, by means of different pathways or mechanisms, the generation or transfer of melanin comprehensively and from multiple angles. The composition can significantly inhibit tyrosinase activity and inhibit the production of melanin, and the active ingredients complement each other and achieve synergistic efficacy. In addition, the composition can also enhance the water solubility of artemisinic acid and reduce the local irritation toxicity caused by the insolubility of artemisinic acid, thereby making the artemisinic acid have good water solubility and low local irritation toxicity while achieving the whitening effect, and promoting deep absorption by the skin.

To provide a new anti-gray hair composition, a melanin production-promoting composition and a tyrosinase activity-enhancing composition.SOLUTION: To provide an anti-gray hair composition, a melanin production-promoting composition and a tyrosinase activity-enhancing composition each containing a hamamelis extract or a eucalyptus extract. The anti-gray hair composition, the melanin production-promoting composition and the tyrosinase activity-enhancing composition are preferably further used in combination with a paulownia leaf extract.SELECTED DRAWING: Figure 1

The invention provides a composition with whitening, tightening and dull yellow improving effects and a preparation method thereof, the composition comprises raw materials such as nicotinamide, tranexamic acid, glycyrrhiza glabra root extract, biological tightening peptide and the like, and auxiliary components such as grape seed extract, modified hyaluronic acid and the like can also be added. The biological tightening peptide is prepared from palmitoyl tripeptide-5, palmitoyl tetrapeptide-7 and acetyl hexapeptide-8 according to a specific ratio. The preparation method comprises the steps of water phase preparation, active component dispersion, bioactive component addition, homogeneous stabilization and the like. Experiments show that the composition can efficiently inhibit tyrosinase activity and melanin synthesis and promote generation of elastin and collagen, is high in oxidation resistance and good in transdermal absorption effect, can be used for preparing cosmetics and has a good application prospect.

The invention discloses a Weissella sinus strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of cosmetics. The strain can be used in the field of cosmetics, has excellent HAS inhibition capability, also has remarkable DPPH and ABTS free radical scavenging capability, and can realize a good antioxidant effect; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of various matrix metalloproteinases can be remarkably inhibited, and a certain effect of reducing cell senescence markers is achieved; in addition, the fermentation product filtrate can inhibit the activity of tyrosinase in vitro, can also significantly inhibit the generation of melanin in a zebra fish model, and has the potential of being used as a tyrosinase inhibitor or a melanin generation inhibitor; the fermentation product filtrate can also significantly inhibit the secretion amount of a cell inflammatory factor NO, has a good soothing effect, and has a certain ability to regulate the skin micro-ecology.

The invention discloses a method for preparing rare saponin based on compound lactobacillus fermentation of black ginseng, a product, a preparation and application, and belongs to the technical field of traditional Chinese medicinal material fermentation. The technical problem to be solved is to provide the preparation method of the rare saponin (Rg3, Rg5, Rk1 and Rh4) compound, the content of the rare saponin is obviously increased after compound lactic acid bacteria treatment, the SOD activity of UVB-induced HaCat cells is obviously improved, the MDA content is obviously reduced, and the melanin content and tyrosinase activity in alpha-MSH-induced B16 cells and zebra fish are reduced. The key point of the technical scheme is as follows: the compound lactic acid bacteria consist of lactobacillus plantarum ACCC11095, lactobacillus casei CGMCC 1.29, lactobacillus rhamnosus ATCC7596 and lactobacillus acidophilus ATCC4356.

The invention discloses a composition containing 4-butylresorcinol as well as a preparation method and application of the composition, and belongs to the technical field of biological fermentation and cosmetic raw materials. The composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate, butanediol, isoprene glycol and water, wherein the composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate; wherein the bacillus subtilis lipopeptide sodium is prepared by fermenting bacillus subtilis H01 and has excellent surface activity, emulsifying property and antibacterial activity. The composition realizes efficient whitening by synergistically inhibiting skin tyrosinase activity and reducing melanin content, is good in component compatibility and high in stability, and can be widely applied to preparation of whitening and skin care products.

The application belongs to the technical field of cosmetics, and particularly relates to a composition with whitening and lightening effects and application thereof. The application can significantly inhibit tyrosinase activity and effectively reduce the amount of melanin generation, indicating that the application has the whitening effect of inhibiting melanin. At the same time, human skin patch tests show no irritation and sensitization, indicating that the application is mild and safe. The application has the effects of safety, whitening and lightening, and is lower in irritation compared with existing products.

This invention discloses a whitening composition containing PDRN and SOD enzymes. The composition includes Selaginella tamariscina extract, mulberry root extract, *Strombus haematocephala* extract, and a nucleotide protein complex, wherein the nucleotide protein complex includes PDRN and SOD enzymes. The composition obtained by scientifically combining Selaginella tamariscina extract, mulberry root extract, *Strombus haematocephala* extract, and the nucleotide protein complex exhibits a significant inhibitory effect on tyrosinase activity and is safe and highly effective, making it widely applicable in the cosmetics field.