191results about How to "Simplify the screening process" patented technology

The present invention provides a process for preparing cross-linked sodium hyaluronate microspheres capable of being adopted as an emboliaztion agent by adopting sodium hyaluronate as a raw material. The process comprises the following steps: preparing a sodium hyaluronate alkaline solution gel with a concentration of 10-30% g/ml; adding the sodium hyaluronate alkaline solution gel to an emulsifier-containing oil phase, and carrying out high speed emulsification through a shearing machine, wherein an emulsification speed is 500-2000 rpm, and a time is 10-20 min; adding a certain amount of a cross-linking agent, stirring for 4-6 h at a room temperature, carrying out a cross-linking reaction, and standing overnight after completing the reaction, wherein a mass percentage of the cross-linking agent in the oil phase is 0.2-2%; and adopting a water-soluble organic solvent to wash to remove the oil phase remained on the surface of the microspheres, and finally drying to obtain the cross-linked sodium hyaluronate microspheres. According to the present invention, the preparation process is simple; and the size of the obtained microspheres is suitable for routine blood vessel emboliaztion, and the obtained microspheres have characteristics of controllable particle size, good microsphere shape, easy screening, elasticity, expandibility, no toxic-side effect on human body, good biocompatibility, good biodegradability, and ensured clinical safety.

The invention provides mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and a method for producing poly(malic acid) by using the mutant through fermentation. The strain is the mutant for producing beta-poly(malic acid) with high yield, which is cultured by multiple groups of composite mutagenesis technology based on beta-poly(malic acid) producing TKPM10017 screened in the soil of Wang Taibao orchard, Yinchuan, Ningxia Hui Autonomous Region, China. Under the optimization condition, the yield of beta-poly(malic acid) reaches 18.4+/-1.3g/l.

A soybean screening device with a drying function, including a main body, a second straight rack and a first straight rack are respectively arranged on the upper and lower inner walls of the zigzag frame, and incomplete gears are arranged inside the zigzagging frame, and the incomplete gears are connected to There is a driving device, the incomplete gear meshes with the second straight rack or the first straight rack, and the incomplete gear is connected with a driving device; there is a fan at the bottom of the circular frame, and a rotating wheel is installed on the inner wall on the right side of the body. A cam is set on the runner on the coaxial center, and the right end of the connecting rod is hinged on the edge of the runner; a sieve plate is pierced on the body, and the sieve plate is located below the cam; The second collection tank, the left end of the sieve plate is located inside the collection tank; the rotation of the cam drives the sieve plate to vibrate up and down to prevent soybeans from clogging the mesh of the sieve plate. points, to speed up the falling of smaller soybeans into the first collection tank, which is simple and practical, convenient and efficient.

The invention discloses a method for screening microorganism high-polymorphism molecular marker sites. The method comprises the following steps of uniformly mixing microspecies of different organismsat equal amount, and extracting total nucleic acid; building a high-flux sequencing library; finding variation sites on a genome; screening the high-polymorphism sites in a gliding translation way; designing multiple amplification primers at both sides of a candidate site; screening the multiple primers, so as to obtain the novel high-polymorphism molecular marker sites. The method has the advantages that in theory, all available high-polymorphism molecular marker sites on the genome can be screened at one time, and be directly used for high-flux detection of variety, and the repeated demonstration process in the traditional screening method is not needed; the screened molecular marker sites can be detected in batch, and the respective molecule amplification and detection on each molecularmarker site are not needed, so that the detection speed is accelerated, and the accuracy is improved; the screened molecular marker has high polymorphism and good resolution, the screening process issimple and quick, and the process is standard.

The invention relates to a single-chain antibody against fenitrothion and a preparation method thereof. A ribosome display library of a whole set of single-chain antibody genes against the fenitrothion is constructed by using a ribosome display technology and rounding a hybrid tumor technology, and three single-chain antibody genes with high affinity and high specificity for the fenitrothion are screened from the library. After the single-chain antibody genes are connected with vectors PPOW3.0 and then subjected to soluble expression in escherichia coli, three soluble proteins with molecular weights of about 30KDa are obtained; and by enzyme-linked immuno sorbent assay (ELISA) and Biacore analysis, the three antibody proteins have high affinity and high specificity for the fenitrothion, so excellent single-chain antibody genes and antibody proteins are provided for establishing ELISA and immune sensors. The invention has significance for realizing quick, simple, convenient and accurate detection of organic phosphorus pesticide residue, and provides a new direction.

The invention provides fowlpox virus vector shuttle plasmid pTGP3 which comprises recombinant arms TKL and TKR, a bidirectional promoter PE/L, a fluorescent protein expression cassette, and a resistant marker gene and replication origin ori; the upstream and the downstream of the bidirectional promoter PE/L are respectively provided with cloning sites MCSL and MCSR; and both ends of the fluorescent protein expression cassette are provided with loxp sequences. The plasmid of the invention has two different screening markers, and the recombinant fowlpox virus prepared with the plasmid can express 1 to 3 types of gene with different meshes in the whole processes of the early and the later periods; the strong composite promoter with expression activity in the early and the later periods is applied so as to realize the all-process high-efficiency expression of a target gene; and the loxp sequences are introduced into both ends of the fluorescent protein expression cassette, so as to knock out the exogenous recombinant fowlpox virus screening markers. The invention lays foundation for the series and the scale application of the recombinant fowlpox virus in vaccine and biological drug research and development fields.

Methods of detecting individuals at risk for atherosclerosis and related vascular diseases involving the detection of IL-1α autoantibodies, as well as therapeutic methods to prevent or treat atherosclerosis and related vascular disease by administering a pharmaceutical composition comprising IL-1α autoantibodies.

The invention relates to a biodegradable sludge-compost fermentation conditioning agent. The sludge-compost fermentation conditioning agent comprises an organic conditioning agent and decomposed sludge with the mass ratio of 1:4-10. In order to promote decomposing of compost, accelerate heating of compost, shorten fermentation period and effectively reduce odor for promote smooth performing of the fermentation process, a sludge compost additive can be added according to the circumstance. The addition amount of the sludge compost additive is 0.05%-1% by weight of decomposed sludge. The conditioning agent is biodegradable, free of residue, free of secondary pollution, high in mechanical strength and good in water adsorption property, is capable of effectively improving the sludge compost structure and improving oxygen diffusion efficiency, and also is recyclable through screening, helps to reduce the usage amount of the fresh conditioning agent and reduce sludge processing cost, and has good environment benefit and considerable economic benefit.

The invention relates to a decreasing DNA library concentration based aptamer screening method and an aptamer. The method includes: a protein-magnetic bead crosslinking step, i.e. subjecting control protein and target protein to crosslinking with magnetic beads respectively; a first-round screening step, i.e. adding a target protein-magnetic bead composite into an initial DNA library solution to conduct first-round screening; a repeated screening step, i.e. diluting the screening product of the previous round by 2-10 times to serve as the DNA library solution of the next round screening so as to carry out the second to the N-th round screening; and a DNA synthesis step, i.e. conducting cloning and sequencing on the final screening product, and synthesizing a numerically superior DNA sequence with high affinity, thus obtaining the aptamer. The method provided by the invention takes low concentration as a screening condition to screen out high affinity aptamer that can bind with the target protein under an extremely low concentration, thus greatly reducing the influence of PCR conditions on the screening process, significantly shortening the screening time, reducing the screening round number, and improving the success rate of screening.

The invention which belongs to the solid waste resource field relates to a cyclically degradable granulated conditioning agent and an application of the conditioning agent in sludge compost. Problems of large dosage, difficult recycle, sludge compost cost increase, slow late degradation and compost period prolongation of traditional organic conditioning agents are solved, and problems of the residual of an inorganic conditioning in the compost and finished product quality reduction are simultaneously solved. The cyclically degradable granulated conditioning agent of the invention is a wood homogeneous body with particle sizes of 0.5-10cm. The application comprises the following steps: 1, mixing sludge with the conditioning agent to obtain a mixture; 2, fermenting the mixture at a high temperature; and 3, discharging the obtained material after the fermentation, and screening out the conditioning agent, wherein the screening rate is greater tan 98%. The conditioning agent of the invention has the advantages of stable space structure, good fluidity, easy screening, recyclable use, good hydroscopicity, water adjustableness, good microbial adhesiveness, recyclable inoculation, and no influence of the finished product quality by the residual. The conditioning agent has very high popularization and application values.

This invention relates to a mono-chain antibody-humanscFv-Fc embedded antibody and its preparation, specialized by hybrid tumor cell strain prepared by surface embedded immune method to transfect mammary animal cell CHO with GS low expression in form of mono-chain embedded antibody gene with pEE14 as recombination antibody carrier to screen CHO cell strains of recombination antibody. The embedded antibody molecule consists of two similar recombined mono-chains, of which each has an antigen combining region connected with heavy and light chains to form antibody to identify antigen. It is smaller a third than a complete antibody due to constant regions CHI structure zones of light and heavy chains removed, so that it is easier to penetrate into tumor and is not easy to be degraded like protein peptide too soon.

The invention relates to EV71 virus-like particles and hand-foot-and-mouth disease vaccine prepared from the EV71 virus-like particles. The EV71 virus-like particles are prepared according to the following steps: (1) constructing recombinant plasmid: respectively connecting pGAPZ alpha A plasmid with P1 protein gene of intestinal EV71 virus and 3CD protease gene to construct P1-pGAPZ alpha A and 3CD-pGAPZ alpha A recombinant plasmid; (2) transferring the recombinant plasmid to expression bacterial strains: sequentially transferring the recombinant plasmid to Pichia pastoris SMD1168 expression bacterial strains to obtain P1-pGAPZ alpha A-3CD-pGAPZ alpha A-SMD1168 recombinant expression strains; and (3) culturing thallus and purifying the EV71 virus-like particles: culturing the Pichia pastoris recombinant expression bacterial strains, centrifugalizing to separate supernate, and carrying out sucrose density gradient centrifugation on precipitation of the supernate after ultracentrifugation, thus obtaining the EV71 virus-like particles. In the invention, the EV71 virus-like particles are easy to obtain through thallus culture, have good stability, are easy to purify and suitable for preparing vaccine, and are convenient for industrial production.

The invention discloses a continuous traceless gene knockout method for corynebacterium glutamicum and belongs to the technical field of gene engineering. The continuous traceless gene knockout method is characterized in that an exonuclease-recombinase-Cre/loxP continuous traceless knockout system, preferably an RecET-Cre/loxP continuous traceless knockout system, is built in corynebacterium glutamicum. According to the method, mediation of plasmids is not required by homologous recombination in corynebacterium glutamicum, and under assistance of RecET, transformation into linear double-stranded DNA is only required; only one round of transformation is required to finish traceless knockout of target genes, the whole traceless knockout process only needs 4-6 days, and meanwhile, continuous traceless gene knockout can be finished; and as a resistance gene is utilized as a selection marker, the selection process is simple and effective, and compared with other traceless knockout methods for corynebacterium glutamicum, the continuous traceless gene knockout method provided by the invention is simpler, more effective and more time-saving.

The invention provides a screening system and screening method for reactive metal powder. The screening system comprises a first vibrating screen device, an airflow grading device, a second vibratingscreen device and a protective gas circulating system, wherein the airflow grading device comprises a spiral feeder and a grading bin, a discharging port of the first vibrating screen device is connected with the spiral feeder, the spiral feeder is connected with the grading bin through a conveying pipe, and a discharging port of the grading bin is connected with the second vibrating screen device. According to the screening system and screening method, the screening process of the screening system is simple, powder screening among different particle size ranges can be completed at one time, screening efficiency and screening precision are improved, and then the technical problems that the screening process of a screening device in the prior art is tedious, and the screening efficiency andthe screening precision are not high are solved.

This invention provides an optical selecting amidase filtration method. The sample detected is dissolved in solution of pH 5 to 9; hydroxylamines are added and make its final concentration between 0.5 to 1M. Then chiral S-amide or R-amide is added as substrate, and make its concentration between 5 to 30mM. They react in water at 20 to 50 degree centigrade, and then the reacted transformation liquor is added solution with Fe<3+> to colored. If it is reddish brown, it reveals that the sample's optical selection is consistent with the substrate spatial configuration, or the amidase didn't have the optical selection. If it is faint yellow, it reveals that the sample's optical selection is not consistent with the substrate spatial configuration. The substrate is don't need derivation, the method can use simple colorimetry to quickly identify the amidase's activity and optical selectivity of the detected microorganism and its extract. Its filtration process is simple, fast, and its equipment request is low, versatility is strong, so it brings great convenience to the optical selecting amidase filtration.

The invention provides a pressure pulp screening machine which comprises a shell, wherein a screen drum and a rotary wing are arranged in the shell, the rotary wing is matched with the screen drum, the rotary wing and the screen drum move relative to each other in a circumferential manner, and a pulp inlet and a good pulp outlet are arranged on the shell; and the pressure pulp screening machine is characterized in that: a spiral dreg discharging machine is arranged on the shell close to a dreg discharging end of the screen drum along the conveying direction of light dregs in a connecting way;a dreg inlet of the spiral dreg discharging machine is connected with the shell nearby the dreg discharging end; and a dreg outlet of the spiral dreg discharging machine is communicated with the outside. When the pressure pulp screening machine in the invention is adopted, the light dregs can be removed in the form of dry dregs without stopping the pressure pulp screening machine.

The invention provides a fully-automatic positioning and sectioning type waste tobacco rod recycling machine. The machine comprises an automatic feeding device, a waste tobacco rod sorting device, a tobacco rod sectioning device and a vibration screening device. The waste tobacco rod sorting device further comprises a plurality of cylindrical guide rails, a conveying belt and a tobacco rod rolling device. The parallel cylindrical guide rails obliquely arranged on a rack are arranged below the tail end of an arc-shaped guide groove of the automatic feeding device. The conveying belt is arranged below the cylindrical guide rails and parallel to the cylindrical guide rails. The distance between the conveying belt and the cylindrical guide rails ranges from 0.5 mm to 5 mm. The tobacco rod rolling belt is arranged on the upper portions of the cylindrical guide rails. The tobacco rod sectioning device is arranged below the cylindrical guide rails and comprises a cutter roller perpendicular to the cylindrical guide rails. The vibration screening device is arranged below the cylindrical guide rails. The fully-automatic positioning and sectioning type waste tobacco rod recycling machine has the advantages that waste tobacco rods are limited through the cylindrical guide rails, the sectioning position is precise, the tobacco rods are not overly smashed, the cigarette paper is complete after the tobacco rods are sectioned, the tobacco rods are screened clearly and easily, the recycled tobacco rods are high in quality, the sectioning depth of the waste tobacco rods is adjustable, and the recycling machine is high in automation degree.

The invention belongs to the technical field of screening of agricultural germplasm resources, and particularly relates to a screening method for a selenium-enriched rice variety. According to the method, preliminary screening is performed at first through indoor cultivation, so that the blindness of the subsequent field screening test is reduced; South Henan black glutinous rice is used as a contrast material, so that the screening process is simple and feasible. In the indoor screening process, a selenium-enriched culture medium is used to provide a uniform and stable selenium source for rice seedlings; the accuracy of variety screening can be substantially improved; seeds are started to be cultivated in the initial stage of germination and growth; nutrition is mainly supplied by the seeds to the seedlings; the culture medium is relatively easy to prepare, and the screening method is easy to apply.

The invention discloses pseudomonas putida WP07, a preparation method and purpose. The pseudomonas putida WP07 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.17760. By a microbiological method, pigwash oil is converted into biodegradable plastic PHA which has environment affinity, so that resource utilization and innocent treatment of the pigwash oil can be realized, the preparation cost of the PHA can also be reduced, and the pseudomonas putida WP07 has favorable application prospects.

The invention discloses a method for quickly screening taxol-producing endogenetic fungi. The method quickly screens the taxol-producing endogenetic fungi by adoption of key enzyme genes synthesized by taxol as molecular markers and on the basis of the PCR amplification technology. The method designs primers according to conserved regions of 10-Deacetyl Baccatin III-10-O- acetylase genes and C-13phenylalanine side chain CoA acetylase genes of reported yew, performs amplification screening on fungi which is obtained by separation of yew barks in turn, performs fermentation culture on the fungi which can generate the two genes by means of amplification, extracts the taxol, analyzes the taxol of the fungi by HPLC and MS, and finally determines the taxol-producing endogenetic fungi. The invention provides an effective strain separating and screening method and a batch of effective original strains in order to realize replacement of extraction and conversion of the taxol from yew plant tissue by the microorganism fermentation method.

The invention discloses a method for screening root media by using tobacco sterile seedlings. The method comprises the following steps: A, sterilizing tobacco seeds; B, cultivating the sterile seedlings; C, shearing the sterile seedlings; D, allocating a plurality of root media to be screened; and E, screening the optimal root media. The method is simple in process, labor-saving and time-saving, economic and reliable, and can shorten the screening period effectively, simplify the screening program, and save the screening material; and the sterile seedlings materials are obtained by seeds in a culture vessel directly, and enter the stage of root screening directly, which omits the trouble of cultivating adventitious buds as compared with the conventional screening test, so that manpower, material resources and time are saved. The tobacco sterile seedlings are adopted to screen the media, and the tobacco sterile seedlings screening can synchronize with the callus induction, so that the test schedule is speeded up. Besides, the induction effect of the method is consistent with that of a method for screening root media by using adventitious buds.

The invention provides a method for performing field screening on a maize inbred line with strong high-temperature resistance. The method comprises the following steps: I, selecting a plurality of inbred lines, and taking an inbred Zheng 58 as a control group; II, seeding each of the selected inbred lines in a test site according to a seeding time I and a seeding time II, wherein the seeding time I is a high-temperature-avoiding period of a maize anthesis maturity period, and the seeding time II is a period when the maize anthesis maturity period is positioned at a high temperature; III, calculating a comprehensive high-temperature resistance coefficient of each inbred line; and IV, determining the high-temperature resistance of the inbred lines. According to the technical scheme of the invention, the method disclosed by the invention has the beneficial effects that (1) the screening process is simplified and is convenient to operate; (2) two traits such as seed setting and single ear yield of the maize are tested and expressed in a digital manner, the evaluation standard is intuitive, and the result is reliable; and (3) the comprehensive high-temperature resistance coefficient of each maize inbred line can be rapidly calculated by using a calculating expression of the comprehensive high-temperature resistance coefficient, and rapid and high-efficiency screening and identifying effects are achieved.

The invention belongs to the technical field of microfluid control, and discloses a multichannel high-flux microfluidic chip for screening drugs for controlling plant diseases. The microfluidic chip comprises a chip body, wherein the chip body comprises a gas control layer, a pesticide feed layer and a pathogen culture layer which are bonded together; the pesticide feed layer comprises a feed channel unit, a concentration gradient unit and a liquid delivery unit; the feed channel unit is connected with an external pesticide source and used for acquiring the pesticide to be screened; the concentration gradient unit is connected with the feed channel unit, and used for acquiring the pesticide to be screened and diluting the pesticide to be screened to form the concentration gradient multichannel dilute liquid; the liquid delivery unit is connected with the concentration gradient unit, and used for acquiring the multichannel dilute liquid and delivering the multichannel dilute liquid to the pathogen culture layer; and the gas control layer is connected with the feed channel unit, and used for controlling the on-off state of the feed channel and controlling the pesticide to be screened to enter the chip body. The invention provides a pesticide screening chip which has the advantages of high efficiency, low cost and low consumption.