Multichannel high-flux microfluidic chip for screening drugs for controlling plant diseases

Abstract

The invention belongs to the technical field of microfluid control, and discloses a multichannel high-flux microfluidic chip for screening drugs for controlling plant diseases. The microfluidic chip comprises a chip body, wherein the chip body comprises a gas control layer, a pesticide feed layer and a pathogen culture layer which are bonded together; the pesticide feed layer comprises a feed channel unit, a concentration gradient unit and a liquid delivery unit; the feed channel unit is connected with an external pesticide source and used for acquiring the pesticide to be screened; the concentration gradient unit is connected with the feed channel unit, and used for acquiring the pesticide to be screened and diluting the pesticide to be screened to form the concentration gradient multichannel dilute liquid; the liquid delivery unit is connected with the concentration gradient unit, and used for acquiring the multichannel dilute liquid and delivering the multichannel dilute liquid to the pathogen culture layer; and the gas control layer is connected with the feed channel unit, and used for controlling the on-off state of the feed channel and controlling the pesticide to be screened to enter the chip body. The invention provides a pesticide screening chip which has the advantages of high efficiency, low cost and low consumption.

Description

technical field [0001] The invention relates to the technical field of microfluidic control, in particular to a multi-channel high-throughput micro-control chip for screening drugs for preventing and controlling plant diseases. Background technique [0002] Plant diseases are the main cause of plant and crop disasters, seriously affecting crop production. Among them, plant anthracnose is a typical disease caused by plant pathogenic fungi. It can cause anthracnose disease of a variety of cruciferous economic crops including cabbage, cabbage, radish, etc., causing significant economic losses. [0003] At present, the control of plant diseases is mainly based on pesticide control, but as the disease resistance of pathogenic bacteria becomes more and more serious, it is imminent to find a convenient, efficient and fast pesticide screening method. [0004] Conventional pesticide screening methods are carried out on petri dishes, and manual operations are repeated, resulting in ...

AI Technical Summary

Problems solved by technology

[0005] The invention provides a multi-channel high-throughput micro-control chip for screening and preventing plant disease drugs, which solves the technical problems of high sample consumption, high cost, heavy workload and low efficiency in drug screening in the prior art

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