Method for rapid screening of Bacillus spp with antibacterial activity
A technology for bacillus and antibacterial activity, applied in the field of rapid screening of bacillus with antibacterial activity, can solve the problems of complex screening methods, high screening labor intensity, long screening time, etc., and achieve low screening cost, simplified screening procedures, and high efficiency Screening effect
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Embodiment 1
[0029] The screening of the bacillus of embodiment 1 anti-escherichia coli
[0030] (1) Weigh 25.0g of the intestinal contents of the pig, add it into a conical flask containing 225mL sterile normal saline, shake it well at 37°C for 20min; In a water bath, treat in a water bath for 30 minutes, shake the Erlenmeyer flask 5 times during the water bath, then dilute the high-temperature-treated sample with sterile saline, and spread the 104-106-fold diluted bacterial suspension on the surface of the nutrient agar medium ;
[0031] (2) The composition of nutrient agar medium is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g, distilled water 1000mL, pH7.4±0.2;
[0032] (3) The culture condition of bacillus is: culture temperature is 37 ℃, culture time is 12h;
[0033] (4) Inoculate the indicator bacterium Escherichia coli O157:H7ATCC43889 preserved in the slope into the LB liquid medium, the culture temperature is 37°C, and the culture time is 12h;
[0034] (...
Embodiment 2
[0039] Example 2 Screening of Bacillus against Listeria monocytogenes
[0040] (1) Weigh 25.0g of soil sample, add it to a conical flask containing 225mL of sterile saline, shake it well at 37°C for 20min; then place the conical flask containing the sample in a water bath at 80-90°C, Treat in a water bath for 30 minutes, shake the Erlenmeyer flask 5 times during the water bath, then gradiently dilute the high-temperature-treated sample with sterile saline, and spread the 104-106-fold diluted bacterial suspension on the surface of the nutrient agar medium.
[0041] (2) The composition of nutrient agar medium is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g, distilled water 1000mL, pH7.4±0.2;
[0042] (3) The culture condition of bacillus is: culture temperature is 37 ℃, culture time is 12h;
[0043] (4) Inoculate the indicator bacterium Listeria monocytogenes CMCC54002 preserved in the slope into the BHI liquid medium, the culture temperature is 37°C, and...
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