Lactobacillus reuteri and application thereof

A technology of Lactobacillus reuteri and bacteria agent, applied in the field of probiotics, can solve the problems of human and livestock intestinal flora disorder, endogenous infection of livestock and poultry, public environment pollution, etc., and achieve good probiotic potential, strong inhibitory effect, The effect of promoting digestion of fiber

Active Publication Date: 2021-02-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The biggest disadvantage of antibiotic use is the emergence of drug-resistant strains, followed by a series of problems such as drug residues, i...

Method used

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  • Lactobacillus reuteri and application thereof
  • Lactobacillus reuteri and application thereof
  • Lactobacillus reuteri and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Isolation and Identification of Lactobacillus reuteri (Lactobacillus reuteri) SLZX19-12

[0059] 1. Isolation of strain SLZX19-12

[0060] Lactobacillus reuteri (Lactobacillus reuteri) SLZX19-12 was isolated from the excrement of Tibetan pig breeding cooperatives in Longzi County, Shannan City, Tibet Autonomous Region. The specific isolation method is as follows:

[0061] 1. Isolation of bacterial strains: take 3g of Tibetan pig feces samples, and carry out gradient dilution with sterile saline, and select 0.1mL of a suitable dilution in a solution containing 0.5% light CaCO 3 Spread evenly on the MRS agar, culture anaerobically at 37°C for 48 hours, randomly select a single colony on the plate according to the size of the calcium-dissolving circle and the colony shape (size, color, etc.), number them, and inoculate them on the MRS solid plate to streak and purify. .

[0062] 2. Preliminary screening: Pick a single colony and inoculate it into an M-MRS test ...

Embodiment 2

[0074] Example 2 Characteristic Detection of Lactobacillus reuteri SLZX19-12

[0075] Activation of the seed solution: Inoculate Lactobacillus reuteri SLZX19-12 into the sterilized MRS liquid medium, let it stand anaerobically at 37°C for 14 hours, then take it out for use.

[0076] 1. Determination of growth curve

[0077] According to the inoculum amount of 1%, draw a certain amount of bacterial liquid from the seed liquid and inoculate it into the MRS liquid medium, put it into an anaerobic workstation, and culture it statically at 37°C. Use a microplate reader to measure the OD of the fermentation broth every 2 h 600 value, and save the fermentation broth of each time period, and carry out the count of viable bacteria.

[0078] The results show( image 3 and Figure 4 ), the Lactobacillus reuteri SLZX19-12 strain entered the logarithmic growth phase at 4h, entered the plateau phase at 14h, and the maximum number of viable bacteria reached 1×10 8 CFU / mL.

[0079] 2. A...

Embodiment 3

[0099] Example 3 Effect of Lactobacillus reuteri SLZX19-12 on intestinal health of mice

[0100] 1. Experimental method

[0101] 1. Select 18 C57BL / 6J male mice of about 12 g, and randomly divide them into three groups (the Con group is the control group, fed with sterile saline; the SL1344 group is the pathogenic bacteria group, fed with no Bacterial saline, on the 15th day, 1×10 6 CFU / mL Salmonella SL1344 bacterial liquid; H_L.reuteri+SL1344 group is the Lactobacillus reuteri SLZX19-12 prevention group, and the concentration of feeding on the first 1-14 days is 1×10 9 CFU / mL of Lactobacillus reuteri bacteria liquid, fed 1×10 on the 15th day 6 CFU / mL Salmonella SL1344 bacteria liquid), 6 mice in each group.

[0102] 2. Gavage method: on the 1st to 14th days, 200 μL was administered once every two days, and 200 μL of Salmonella typhimurium was administered once on the 15th day, and chyme and tissue samples were collected 7 days later.

[0103] The experimental mouse room w...

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Abstract

The invention relates to the technical field of probiotics and particularly relates to lactobacillus reuteri and an application thereof. The invention provides Lactobacillus reuteri SLZX19-12 which ispreserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Lactobacillus reuteri SLZX19-12 is CGMCC No. 20121. Lactobacillus reuteri SLZX19-12 can be planted in an animal body, and is high in acid resistance and cholate resistance; the good inhibition effect is achieved on escherichia coli and salmonella; feruloyl esterase can be generated, and release and digestion of fibers in the feed in digestive tracts are promoted; the lactobacillus reuteri SLZX19-12 is prepared into a microbial inoculum and then fed to animals, intestinal floracan be improved, intestinal immunity can be improved, intestinal health can be improved, and the effect is safe and reliable.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a Lactobacillus reuteri and an application thereof. Background technique [0002] With the widespread use of antibiotics, their disadvantages in animal husbandry are gradually revealed. The biggest disadvantage of the use of antibiotics is the emergence of drug-resistant strains, followed by a series of problems such as drug residues, intestinal flora disturbance in humans and animals, endogenous infections and superinfections in livestock and poultry, and public environmental pollution. The overuse of antibiotics has caused serious threats to human health, which has aroused widespread concern and attention around the world. It is of great significance to find green, safe, efficient and non-toxic alternatives to antibiotics. [0003] At present, although probiotics have only been used in the feed industry for more than 20 years, they have shown great potential in breeding an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/18A23K10/18A61K35/747A61P31/04A61P31/12A61P1/00C12R1/225
CPCC12N1/20C12N9/18A23K10/18A61K35/747A61P31/04A61P31/12A61P1/00C12Y301/01073C12R2001/225C12N1/205Y02A50/30
Inventor 马曦吴建民王娴赵莹鲁琳
Owner CHINA AGRI UNIV
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