41 results about "Antibiotic use" patented technology

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

This invention belongs to the field of microbial technology and discloses a strain of *Citrobacter freundii* bacteriophage PRF337, its bacteriophage composition, and its applications. The *Citrobacter freundii* bacteriophage PRF337 was deposited on May 26, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46489. The bacteriophage PRF337 exhibits broad-spectrum bactericidal activity against *Citrobacter freundii*, capable of lysing *Citrobacter freundii* strains containing different virulence genes and from different sources. It also possesses certain temperature and pH stability and can be used as an active ingredient in pharmaceutical preparations, environmental disinfectants, and water purifiers. It can effectively control *Citrobacter freundii* infection in aquatic animals such as fish and shrimp in aquaculture environments and can also be used for the purification of pathogens in aquatic environments. It is safe to use and effectively avoids the problems of antibiotic residues and pathogen resistance caused by antibiotic use.

The invention discloses a method for removing antibiotics by activating permanganate through ball milling MoS2, and belongs to the field of water treatment technologies and environmental functional materials. The method comprises the following steps: co-precipitating to synthesize MgAl-layered double hydroxides, and carrying out hydrothermal compounding and ball-milling bonding to obtain the ball-milling modified composite material and the water treatment application thereof. In order to solve the technical problems that in an existing permanganate activation technology, active species in a homogeneous system are prone to inactivation, the activation efficiency of a heterogeneous system is low, and only physical contact exists between materials, so that an interface electron transfer potential barrier is high, sodium thiosulfate is adopted for assisting ball milling to induce chemical bonding, a continuous electron transfer network is constructed, an electron-rich state is formed on the surface of MoS2, and the MoS2 is prepared. Antibiotics are synergistically oxidized through a high-valence manganese intermediate and a free radical double path, and meanwhile heavy metal is adsorbed through multiple mechanisms of sulfur coordination, sulfur vacancy anchoring and hydroxyl complexing. Under the optimal condition, the method realizes that the SMX removal rate reaches 96.8% and the lead ion removal rate reaches 99.2%.

This application provides a method and system for predicting urinary tract infections related to urodynamic examinations. It addresses the limitations of current preventative measures, where methods with high evidence levels and good efficacy, including antibiotic intravesical instillation and weekly oral cyclic antibiotic therapy, inevitably require antibiotic use. The method includes: acquiring urodynamic examination data from a target patient; analyzing the curve information in the urodynamic examination data to obtain a curve numerical sequence corresponding to a time axis; extracting a urodynamic feature set based on the curve numerical sequence; constructing a medical history feature set based on the target patient's medical history data; inputting the urodynamic feature set and the medical history feature set into a urinary tract infection risk prediction model to output a risk prediction result for urinary tract infection after urodynamic examination. The risk prediction result includes at least a risk score or risk stratification result.

This invention provides the application of Erythrina bark in the preparation of drugs for the prevention and / or treatment of infectious coryza in chickens. The active ingredient of this drug can be composed solely of Erythrina bark, making the formulation easy and convenient to implement. It can be used as a substitute for antibiotics during the egg-laying period of chickens, with no drug residue requirements, reducing the economic loss of discarded eggs after the use of antibiotics in laying hens. It has a high cure rate for infectious coryza, is safe and has no toxic side effects, and is easy to use.

The invention discloses a dairy cow digestive tract and respiratory tract disease therapeutic agent based on compound probiotics and plant extracts, through the synergistic effect of multiple strains and the multiple effects of the plant extracts, the therapeutic effect is remarkably improved, and the use of antibiotics is reduced. The preparation has the advantages of greenness, safety, remarkable curative effect and the like, and is suitable for large-scale popularization and application.

The invention relates to a peptide that can be used as an alternative to existing antibiotics for the inhibition of pathogenic microorganisms and the treatment of infections due to these microorganisms, contributing to the prevention of resistance development arising from antibiotic use by reducing antibiotic use, is safer compared to human-derived natural peptides, has a very low toxicity compared to existing antimicrobial peptides, and also reducing tissue damage and side effects during infection by reducing inflammation due to microbial infections, also having the potential to regulate the immune system and immunomodulation, which will contribute to fight infections by influencing the activation and function of immune cells.

This invention belongs to the field of environmental engineering materials technology and discloses a method for preparing an antibiotic-encapsulated nanofiber membrane biocarrier for the domestication of specific microbial populations. The aim is to achieve the domestication and control of specific microbial populations through the combination of antibiotic encapsulation and nanofiber membranes. This antibiotic-encapsulated nanofiber membrane biocarrier utilizes the high specific surface area and good permeability of the porous, expanded structure of emulsion electrospun nanofiber membranes to effectively immobilize antibiotics and achieve precise regulation of microbial populations through a slow release mechanism. Simultaneously, the microstructure of the nanofiber membrane provides a suitable growth environment for microorganisms, promoting the proliferation and domestication of specific populations. This invention not only improves the efficiency of microbial population domestication but also reduces the amount of antibiotics used, resulting in both environmental and economic benefits. The preparation process of this carrier is simple and convenient, suitable for wastewater treatment, bio-fermentation, and other fields, and has broad application prospects.

The application provides a double-strand DNA vaccine for late Edwardsiella tarda disease, which can significantly induce Th1 type immune bias, enhance fish cell immune response, improve the resistance to intracellular bacterial infection, and has the advantages of safety and high efficiency, easy preparation, strong adaptability for on-site promotion and the like. The double-strand DNA vaccine uses a fusion fragment of an immune adjuvant IL-12, which is constructed by connecting a nucleic acid fragment coding a subunit 12p35 with an amino acid sequence of SEQ ID NO:1 and a nucleic acid fragment coding a subunit 12p40 with an amino acid sequence of SEQ ID NO:3 through a flexible connecting peptide linker. The double-strand DNA vaccine realizes the synergistic expression of OmpC antigen and IL-12 adjuvant, enhances immunogenicity, and solves the problem of insufficient immune effect of a traditional DNA vaccine; and the vaccine has no obvious toxic side effect on fish bodies, is not dependent on the use of antibiotics, is green and environmentally friendly, and has a good industrialization prospect.

Compositions for identifying a pathogenic bacterial infection include a mannitol compound with one or more substituents including radioisotopes. These radiopharmaceuticals, such as a positron-emitting mannitol analogue, [18F]fluoromannitol ([18F]FMtl), are rapidly taken up by gram-positive and gram-negative bacteria such as E. coli, S. aureus, A. baumannii, S. epidermis, P. mirabilis, S. enterica, K. pneumonia, E. faecium, E. cloacae, and M. marinum, but not non-active infection sites such as sterile inflammatory sites, cancer sites, etc. Administration of these radiopharmaceuticals to and subsequent imaging of patients, e.g., via positron emission tomography (PET), enables detection of deep-seated and difficult to manage bacterial infections, such as osteomyelitis and prosthetic joint infection, or in patients with sickle cell disease. [18F]FMtl injection detects and differentiates infection rapidly. The radiolabeled mannitol compounds can be produced via nucleophilic substitution reactions that are deployable on commercially available synthesizers, facilitating straightforward and wide accessibility, and counteracting unnecessary antibiotic use.

The invention relates to the technical field of fermentation engineering, and in particular relates to Enshi L77 for producing polyether antibiotics and application of the Enshi L77. The preservation number of the Ennuli L77 is CGMCC (China General Microbiological Culture Collection Center) No.36394. The The application comprises the following steps: fermenting by adopting the Enshi L77, extracting and separating from a fermentation product and thalli to obtain a polyether compound, and using the polyether compound as an antibiotic. According to the invention, a strain of Enshi L77 is obtained through research and screening, functional verification of the Enshi L77 shows that a polyether compound with relatively good antibacterial activity can be separated from a fermentation product of the Enshi L77, and the Enshi L77 can be used for preparing human or livestock anti-infection drugs and has a relatively good application prospect.

This invention provides the application of anti-glycolytic drugs in the preparation of drugs and / or formulations that enhance the anti-infective efficacy of anti-stress products, belonging to the field of veterinary drug technology. Experiments have shown that anti-glycolytic drugs can enhance the anti-infective efficacy of anti-stress products. This invention combines anti-glycolytic drugs with anti-stress products in a specific ratio, significantly enhancing the anti-infective ability of animals under stress, with an effect no less than that of anti-stress products combined with antibiotics, and can replace antibiotics. The method for enhancing the anti-infective efficacy of anti-stress products provided by this invention is simple, environmentally friendly, and bacteria are less likely to develop drug resistance, showing good application prospects.

This invention relates to a phage lysin LysSPYS1, a composition containing the phage lysin, and their applications. The sequence of the phage lysin LysSPYS1 is shown in SEQ ID No. 1. This phage lysin exhibits highly efficient and broad-spectrum bactericidal activity; it has a lytic effect on a variety of bacteria and can be used as a bacteriostatic agent. Furthermore, combining this phage lysin with polymyxin B can exert a synergistic antibacterial effect, significantly reducing the amount of antibiotics used. The phage lysin can also be used in combination with magnolol to achieve a prominent antibacterial effect.

This invention discloses a reagent for detecting the content of aminoglycoside antibiotics and its application method. The reagent, by volume percentage, comprises 10-20% buffer solution, 8-12% anionic surfactant, 1-5% ANS-Na aqueous solution, and 60-65% ultrapure water. The detection method involves mixing the reagent with a water sample containing aminoglycoside antibiotics, reacting, and then performing fluorescence detection. The detection result is then used to calculate the content of aminoglycoside antibiotics using a standard curve. This invention utilizes the advantages of ANS-Na's high sensitivity, rapid response, and ease of operation. It forms a ternary nanoaggregate with the anionic surfactant and AGs, providing a hydrophobic environment for the small molecule fluorescent substance ANS-Na, thereby enhancing the fluorescence signal and achieving sensitive detection of AGs.

The invention provides a piglet health-care feed additive as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The health-care feed additive for the piglets is prepared from the following raw materials in parts by weight: 30-50 parts of astragalus membranaceus, 30-50 parts of myrobalan, 25-40 parts of cynomorium songaricum, 25-40 parts of rhizoma alismatis, 25-40 parts of cortex lycii radicis, 20-30 parts of roasted rhizoma atractylodis macrocephalae, 20-30 parts of spina date seeds, 20-30 parts of bunge corydalis herb, 20-30 parts of acalypha australis, 10-20 parts of turmeric, 10-20 parts of paulownia leaves, 5-15 parts of loquat leaves, 3-5 parts of folium isatidis and 3-5 parts of hawthorn through processes such as fermentation, freeze drying and the like. The feed additive has the effects of regulating intestinal flora structures of piglets, increasing the number of beneficial bacteria in intestinal tracts, reducing field planting of harmful bacteria, reducing the occurrence rate of bacterial diarrhea of the piglets and the like, can replace antibiotics for use, does not remain harmful substances in pig bodies after being used, and is beneficial to realizing green and sustainable development of piglet breeding.

The present application belongs to the technical field of traditional Chinese medicine, and mainly relates to a traditional Chinese medicine composition for blocking bacterial vitreous body disease of shrimplets, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: chebula 20-60 parts, schisandra 10-40 parts, and cyperus 10-40 parts. The traditional Chinese medicine composition can significantly reduce the mortality of shrimplets suffering from bacterial vitreous body disease (BVS), reduce the number of cultivable bacteria and the number of vibrio in the hepatopancreas of prawns, and has a good repair effect on the damage of the hepatopancreas and intestinal tract of the diseased shrimplets. The traditional Chinese medicine composition is simple and convenient to use, safe and green, can effectively reduce or replace the use of antibiotics, and is suitable for the control of vibrio diseases in the process of shrimplet cultivation and breeding production.

This invention belongs to the field of membrane separation technology and relates to a method for preparing a vermiculite-based two-dimensional layered nanofiltration membrane for antibiotic desalination. The method involves mixing two-dimensional vermiculite nanosheets with a metal polyphenol complex obtained by reacting them with tannic acid and ferric chloride at pH 8.5, followed by vacuum filtration to prepare a membrane. Subsequent alkali treatment removes excess free tannic acid, resulting in a vermiculite-based two-dimensional layered nanofiltration membrane for antibiotic desalination. This invention is the first to introduce the tannic acid-ferric chloride metal polyphenol complex into the sub-nanometer space of the two-dimensional vermiculite membrane, allowing for the control of the interlayer spacing and achieving highly efficient separation of salt and antibiotics using the vermiculite-based two-dimensional layered nanofiltration membrane. The method of this invention allows for the control of the interlayer spacing of the two-dimensional vermiculite membrane and is simple to operate.

The invention discloses a construction method and application of escherichia coli capable of producing fumaric acid, and belongs to the technical field of biological engineering. By-product synthesis related genes such as a fumarase coding gene fumA, a lactic dehydrogenase coding gene ldhA and a pyruvate oxidase coding gene poxB are knocked out, so that the yield of fumaric acid is effectively increased, and the content of heteroacid is reduced. On the basis, the expression of Afpyc, Ecppc and EcaceA genes is up-regulated independently or in a combined manner. The genetically engineered bacterium constructed by the invention adopts an aerobic-microaerobic two-stage fermentation strategy in a 5L fermentation tank, the highest yield of fumaric acid reaches 50.2 g / L after fermentation for 60 hours, the glucose conversion rate is 0.48 g / g, and the by-products lactic acid and acetic acid are both controlled to be 4-5g / L. The strain does not need to carry plasmids, so that the risks of antibiotic use and plasmid loss are avoided, and the strain has good industrial application potential.

Herein, an exploration was made of the potential therapeutic effect of injecting neomycin into the lung immediately after traumatic brain injury (TBI). It was hypothesized that modulation of the pulmonary microbiome may help alleviate neuroinflammation and cognitive impairment following TBI. Traumatic brain injury induced neutrophil infiltration into the alveoli along with interstitial pulmonary edema, and reduced levels of caspase-1, IL-1β, and RAGE. TBI rats treated with neomycin exhibited a significant decrease in Acinetobacter levels in BAL and a decrease in α-diversity Shannon index compared to rats without neomycin. The neomycin-administered TBI rats exhibited a higher discrimination index in a novel object recognition test, indicating that cognitive impairment was alleviated. While serum inflammatory cytokines in injured mice tended to increase for up to 7 days, such increases were not observed from day 3 in neomycin-administered TBI rats. Injection of neomycin into the lung immediately after TBI effectively alleviated neuroinflammation and cognitive impairment through modulation of the pulmonary microbiome.

The invention discloses nucleic acid aptamers for specifically recognizing florfenicol, thiamphenicol and chloramphenicol, and the nucleic acid aptamers are base sequences shown as SEQ ID NO.1 or SEQ ID NO.3 in a sequence table, or base sequences with the homology of more than 70% with the SEQ ID NO.1, or base sequences with the homology of more than 40% with the SEQ ID NO.3. The invention further discloses a preparation method of the nucleic acid aptamers for specifically recognizing florfenicol, thiamphenicol and chloramphenicol. Experiments prove that the nucleic acid aptamer can specifically recognize all amide alcohol antibiotics clinically used by veterinarians, and is not interfered by ampicillin and other antibiotics. The compound has wide application in the fields of food safety, environmental safety and the like, can be used for preparing a sensor or a detection kit for specifically recognizing florfenicol, thiamphenicol and chloramphenicol, and has important significance in preliminary screening of amido alcohol antibiotic residues and pollution in large-batch food and environmental samples; and a new recognition element is provided for establishing a rapid, simple, cheap and sensitive florfenicol, thiamphenicol and chloramphenicol detection method at the same time.

This invention belongs to the field of synthetic biology technology and relates to a poultry bio-compound microbial agent based on high-throughput screening in synthetic biology and its application. This invention constructs a microbial strain library from intestinal environment samples of high-yielding poultry flocks. Under simulated poultry intestinal conditions, high-throughput fermentation and sequencing are used to evaluate the metabolites, pathogenicity inhibition ability, and tolerance of a large number of strains and strain combinations in parallel, screening for functional strain combinations with synergistic effects. Then, the strain ratio and dosage are optimized through poultry animal experiments to produce a poultry bio-compound microbial agent for use in feed or drinking water. The compound microbial agent of this invention can significantly improve poultry growth performance and reduce mortality rates with reduced or no antibiotic use, making it suitable for large-scale green farming promotion.

The invention belongs to the technical field of aquaculture, and particularly relates to a feed mandarin fish fry closed factory whole-process cultivation method based on biofloc in-situ purification. According to the method, biological floc in-situ culture, closed in-situ bait fish culture, mandarin fish juvenile fish in-situ thickening, gradient feed domestication and pathogen isolation monitoring are sequentially completed in a closed culture pond, and stable biological floc is formed by inoculating bacillus, lactic acid bacteria, photosynthetic bacteria and other complex microbial inoculants and regulating and controlling the carbon nitrogen ratio. Water in-situ purification and biological bacteriostasis are realized; in the whole process, no pond is transferred, and no exogenous living bait fish is introduced; the feed domestication is completed by adopting a three-stage gradient feeding scheme, and the domestication period is shortened and the survival rate of the fries is increased by combining the biofloc food calling and nutritional supplement. According to the method, exogenous pathogens can be effectively isolated, the stress of the siniperca chuatsi fries is reduced, the use of water and antibiotics is reduced, and closed, green and efficient factory cultivation of the siniperca chuatsi fries in the whole process from opening to feed domestication is achieved.

This application discloses a spectral synthesis method applied to broiler farming. It generates a synthesized spectrum by combining red and green LEDs, with a green to red light ratio of 2:1 and a color temperature range of 7000K ± 500K. Furthermore, this application also discloses a spectral synthesis device and a broiler farming method. By combining red and green LEDs and adjusting the ratio of green to red light in the spectrum to achieve a 2:1 ratio, this method irradiates the broilers with the synthesized spectrum throughout their entire farming cycle. This stimulates the poultry, maximizing their growth potential, improving immunity, reducing antibiotic use during growth, increasing weight, and lowering the feed conversion ratio, thereby achieving optimal economic benefits.

The invention provides a largemouth bass feed and application thereof. The largemouth bass feed comprises 0.5 * 10 < 9 >-1.2 * 10 < 9 > CFU / g of bacillus velezensis; the feed is applied to breeding of micropterus salmoides, the feeding rate is 2-4% / day, and the breeding temperature is 25-27 DEG C; the pH (Potential of Hydrogen) is 6.8 to 7.6; according to the micropterus salmoides feed, the use amount of antibiotics in aquaculture can be reduced, diseases in the micropterus salmoides culture process can be prevented, the physique and yield of micropterus salmoides can be enhanced, drug resistance risks, environment and food residues and toxic and side effects are avoided, the development requirements of green aquaculture are completely met, and a new technical path is provided for prevention and control of bacterial diseases of aquatic products.

The application discloses a chitin membrane dressing for promoting wound healing and a preparation method thereof, and comprises the following components in parts by weight: a bacteriostatic slow-release gel carrier 36-40 parts, and a bacteriostatic repair and healing-promoting essential oil 6-8 parts; the bacteriostatic slow-release gel carrier is prepared by using modified chitin and modified dextran to prepare a gel and in-situ polymerization of an organic metal framework; and the bacteriostatic repair composition is obtained by extracting cortex phellodendri, wild chrysanthemum, portulaca oleracea, rhubarb, giant knotweed and aloe vera by using tea tree essential oil. The application proposes a method for preparing a gel dressing by using modified dextran and in-situ polymerization of a metal organic framework together with modified chitin, realizes multiple drug release, prolongs the effective time of drug action, replaces antibiotics by using plant extracts, and further realizes the technical effects of avoiding the use of antibiotics and bacteriostasis by multiple mechanisms.

The invention provides a method for removing antibiotics through a contact electro-catalysis-Fenton coupling system, and belongs to the technical field of organic pollutant degradation. According to the method, fluorine-containing organic polymers are added into a reaction system to serve as a catalytic material for a contact electro-catalysis reaction, reactive oxygen species are generated by utilizing electron transfer generated in the contact electrification process of the fluorine-containing organic polymers and water under the ultrasonic action, and Fe < 2 + > is introduced into a contact electro-catalysis system to form a contact electro-catalysis-Fenton coupling system; the introduction of Fe < 2 + > can generate a new electron transfer process and generate more active oxygen, so that the oxidative degradation efficiency of the antibiotics is remarkably improved. Results of the embodiment show that compared with contact electro-catalysis, the degradation rate of the contact electro-catalysis-Fenton coupling system is increased by 2.3-23 times.

Multi-drug resistant (MDR) bacteria potentially pose a significant risk to people in the United States and all over the world; at the moment, the risk is the greatest where antibiotics are most commonly taken. The most notable MDR organisms are Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus faecium (VRE) and Carbapenem-resistant Pseudomonas aeruginosa. All three are associated with nosocomial infections. Embodiments of the present invention are directed to marine microorganism fractions and products for treating multi-drug resistant bacterial infections.

The application discloses a traditional Chinese medicine composition for improving poultry production performance and a preparation method and application thereof. The traditional Chinese medicine composition contains astragalus membranaceus, radix codonopsis, smilax glabra, fried atractylodes, coix seed, dandelion and fried licorice. The traditional Chinese medicine composition can improve the immunity of the body, reduce the use of antibiotics, has no drug resistance, replaces part of functional additives, achieves the effect of reducing cost and increasing efficiency, enhances the immunity of the body, enhances the development of the skeleton, regulates intestinal health, reduces the feed-meat ratio, improves the production performance, is suitable for various poultry, including quails, pigeons, laying hens and broilers, and the like, and can be directly added in feed for use, reduces the process of mixing feed, and saves time and effort.