100 results about "Antibiotic use" patented technology

The invention relates to the technical field of medical data, in particular to an acute infection rapid diagnosis method, device and equipment, and the method comprises the steps: collecting an electronic medical record, blood routine, inflammation indexes, a microbiological detection result and imaging data of a target patient; respectively extracting corresponding target basic features based on the electronic medical record, the blood routine, the inflammation index, the microbiological detection result and the imaging data; based on the basic features, a convolutional neural network and a long-short-term memory network, target depth features are extracted; obtaining an acute infection prediction model, wherein the acute infection prediction model is obtained through model training; based on the target depth feature and the acute infection prediction model, the acute infection type of the target patient is determined, the accuracy and efficiency of diagnosis are further improved, scientific antibiotic use guidance is provided for doctors, waste of medical resources is reduced, and prognosis of the patient is improved.

The invention discloses a method for distinguishing similar antibiotics by using a fluorescent sensing array based on YOLOv5 target recognition. The method comprises the following steps: S1, preparing GMP-Tb solid powder; s2, mixing the GMP-Tb solid powder with a rhodamine B solution in proportion to obtain a multi-proportion fluorescent probe; s3, mixing antibiotics with different concentrations with the multi-proportion fluorescent probe to obtain a fluorescent sensing array; s4, collecting color images in the fluorescent sensing array and dividing the color images into a training set and a test set; s5, constructing an OLOv5 optic nerve model, inputting the acquired color image into a YOLOv5 optic nerve model, and outputting a feature value of the acquired color image; s6, inputting the characteristic value of the color image into a classifier model for prediction classification to obtain a distinguishing result; and S7, inputting a color image formed by mixing the to-be-detected antibiotic and the multi-proportion fluorescent probe into the YOLOv5 optic nerve model to obtain a characteristic value of the color image, and then outputting a distinguishing result through the classifier model.

An antibiotic stewardship program system and method for tracking antibiotic use, in particular, antibiotics in medical facilities. The system uses human and artificial intelligence algorithms to enable the implementation of United States mandated antibiotic management, control, and reporting requirements.

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention provides an antibiotic-free host-plasmid TA maintenance system and application thereof, and relates to the technical field of gene engineering. According to the invention, a toxin gene CcdB is accurately integrated to a host escherichia coli chromosome fhuA site through a gene editing technology, and accurate control of toxin expression is realized by adopting an inducible promoter. The antitoxin gene is simplified to a plasmid skeleton, and the toxin inhibition function is maintained through constitutive expression. According to the method, plasmid-free cells are eliminated through arabinose-induced toxin expression, so that stable maintenance of plasmids is realized (the loss rate is lt; and the method completely avoids the use of antibiotics, meets the biological safety requirements, is suitable for large-scale production of vaccines, recombinant proteins and other biological products, and has the advantages of high plasmid yield, low environmental leakage risk, process compatibility and the like.

The invention discloses a breeding method for improving pork quality by using Chinese herbal medicine fermentation broth, and relates to the field of livestock and poultry breeding, the method comprises the following steps: preparing composite Chinese herbal medicine fermentation broth, and adding the fermentation broth into daily ration in proportion for feeding in the growth and fattening stage of live pigs, the compound Chinese herbal medicine fermentation liquor is prepared by fermenting a Chinese herbal medicine composition containing astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn through a compound microbial agent; according to the breeding method for improving the pork quality by using the Chinese herbal medicine fermentation liquor, Chinese herbal medicines such as astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn fruits are fermented through a compound microbial agent, the deep synergistic effect of the Chinese herbal medicines and probiotics is achieved, the bioavailability of effective components is remarkably improved, and therefore products better meeting high-end market requirements are produced; meanwhile, probiotics and metabolites in the fermentation liquor can effectively optimize the intestinal health of livestock and reduce the morbidity, and a reliable path is provided for reducing or replacing antibiotics.

The invention discloses a preparation method of a recombinant lactic acid bacteria preparation for expressing antibacterial peptide and interleukin 22 (IL-22) and application of a composite microecological preparation composed of the antibacterial peptide and the interleukin 22 in prevention and treatment of animal bacterial infection diseases. The probiotic preparation provided by the invention comprises lactic acid bacteria, antibacterial peptide and interleukin, the recombinant lactic acid bacteria preparation can be taken orally without generating toxicity to an organism, and the recombinant lactic acid bacteria preparation can be colonized in intestinal tracts and secrete the antibacterial peptide and IL-22. The antibacterial peptide secreted by the recombinant lactic acid bacteria can punch bacterial cell membranes to cause bacterial death, does not cause generation of drug-resistant bacteria, and can reduce or replace the use of antibiotics; iL-22 secreted by the recombinant lactic acid bacteria can promote the repair of damaged tissues of intestinal tracts, activate the immune system of the organism and enhance the resistance; the probiotic effect of lactic acid bacteria can regulate the balance of intestinal flora of the organism, promote nutrient absorption and enhance the immunity of the organism. The preparation provided by the invention has the advantages of innovativeness, good safety, no toxic or side effect, simple administration mode, remarkable effect and the like, can effectively reduce the use of antibiotics, and provides a new solution for preventing and treating bacterial diseases in animal husbandry.

The present application relates to the field of biological glass, in particular to a kind of composite biological glass and its preparation method and application.The present application uses rhBMP-2, doped antibacterial ion 45S5 bioactive glass and polyethylene glycol-lactide-glycolide polymer compound preparation slow-release antibacterial biological glass, the rhBMP-2 can occur synergistic effect with doped antibacterial ion 45S5 bioactive glass, compared with homologous growth factor can play more optimal synergy with antibacterial ion, so as to have higher antibacterial property and osteogenesis, while having longer slow-release effect in polyethylene glycol-lactide-glycolide polymer.The composite biological glass provided by the present application overcomes the pain points of the prior art for the treatment of oral bone defect, improves the treatment effect, reduces the amount of antibiotic use, and reduces the risk of infection.

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The invention provides application of an anti-glycolysis drug in preparation of a drug and / or a preparation for improving the anti-infection effect of an anti-stress product, and belongs to the technical field of veterinary drugs. Experiments show that the anti-glycolysis drug can improve the anti-infection effect of the anti-stress product. The anti-glycolysis drug and the anti-stress product are used in cooperation according to a specific proportion, the anti-infection ability of animals in the stress state can be remarkably enhanced, the effect is not lower than the effect of cooperation of the anti-stress product and antibiotics, and the anti-glycolysis drug and the anti-stress product can be used instead of the antibiotics. The method for improving the anti-infection effect of the anti-stress product, provided by the invention, is simple, convenient, green and environment-friendly, bacteria are not easy to generate drug resistance, and the method has a relatively good application prospect.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

This invention belongs to the field of microbial technology and discloses a strain of *Citrobacter freundii* bacteriophage PRF337, its bacteriophage composition, and its applications. The *Citrobacter freundii* bacteriophage PRF337 was deposited on May 26, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46489. The bacteriophage PRF337 exhibits broad-spectrum bactericidal activity against *Citrobacter freundii*, capable of lysing *Citrobacter freundii* strains containing different virulence genes and from different sources. It also possesses certain temperature and pH stability and can be used as an active ingredient in pharmaceutical preparations, environmental disinfectants, and water purifiers. It can effectively control *Citrobacter freundii* infection in aquatic animals such as fish and shrimp in aquaculture environments and can also be used for the purification of pathogens in aquatic environments. It is safe to use and effectively avoids the problems of antibiotic residues and pathogen resistance caused by antibiotic use.

The invention discloses a method for removing antibiotics by activating permanganate through ball milling MoS2, and belongs to the field of water treatment technologies and environmental functional materials. The method comprises the following steps: co-precipitating to synthesize MgAl-layered double hydroxides, and carrying out hydrothermal compounding and ball-milling bonding to obtain the ball-milling modified composite material and the water treatment application thereof. In order to solve the technical problems that in an existing permanganate activation technology, active species in a homogeneous system are prone to inactivation, the activation efficiency of a heterogeneous system is low, and only physical contact exists between materials, so that an interface electron transfer potential barrier is high, sodium thiosulfate is adopted for assisting ball milling to induce chemical bonding, a continuous electron transfer network is constructed, an electron-rich state is formed on the surface of MoS2, and the MoS2 is prepared. Antibiotics are synergistically oxidized through a high-valence manganese intermediate and a free radical double path, and meanwhile heavy metal is adsorbed through multiple mechanisms of sulfur coordination, sulfur vacancy anchoring and hydroxyl complexing. Under the optimal condition, the method realizes that the SMX removal rate reaches 96.8% and the lead ion removal rate reaches 99.2%.

The invention relates to the field of bioglass, in particular to composite bioglass as well as a preparation method and application thereof. The rhBMP-2, the 45S5 bioactive glass doped with the antibacterial ions and the polyethylene glycol-lactide-glycolide polymer are compounded to prepare the slow-release antibacterial bioglass, the rhBMP-2 and the 45S5 bioactive glass doped with the antibacterial ions have a synergistic interaction effect, and compared with homologous growth factors, the slow-release antibacterial bioglass can play a better synergistic effect with the antibacterial ions, so that the slow-release antibacterial bioglass has the advantages that the slow-release antibacterial bioglass is prepared, and the antibacterial activity of the slow-release antibacterial bioglass is improved. Therefore, the antibacterial and osteogenic hydrogel has higher antibacterial property and osteogenic property, and has a longer slow-release effect in a polyethylene glycol-lactide-glycolide polymer. The composite bioglass provided by the invention overcomes pain points generated by oral bone defect treatment in the prior art, improves the treatment effect, reduces the use amount of antibiotics, and reduces the infection risk.

This application provides a method and system for predicting urinary tract infections related to urodynamic examinations. It addresses the limitations of current preventative measures, where methods with high evidence levels and good efficacy, including antibiotic intravesical instillation and weekly oral cyclic antibiotic therapy, inevitably require antibiotic use. The method includes: acquiring urodynamic examination data from a target patient; analyzing the curve information in the urodynamic examination data to obtain a curve numerical sequence corresponding to a time axis; extracting a urodynamic feature set based on the curve numerical sequence; constructing a medical history feature set based on the target patient's medical history data; inputting the urodynamic feature set and the medical history feature set into a urinary tract infection risk prediction model to output a risk prediction result for urinary tract infection after urodynamic examination. The risk prediction result includes at least a risk score or risk stratification result.

An external or topical formulation for treatment or prevention of infections involving body surfaces using a depsipeptide antibiotic drug such as lotilibcin having improved efficacy and safety is provided. The antibacterial effect of lotilibcin tested in infectious models is substantially enhanced by addition of boric acid in a low concentration of an additive level which does not produce any pharmacological activity by itself, thereby offering a formulation for external preparations having improved safety due to a decrease in dose, dosing period, and dosing frequency.

This invention provides the application of Erythrina bark in the preparation of drugs for the prevention and / or treatment of infectious coryza in chickens. The active ingredient of this drug can be composed solely of Erythrina bark, making the formulation easy and convenient to implement. It can be used as a substitute for antibiotics during the egg-laying period of chickens, with no drug residue requirements, reducing the economic loss of discarded eggs after the use of antibiotics in laying hens. It has a high cure rate for infectious coryza, is safe and has no toxic side effects, and is easy to use.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.

This invention relates to the field of poultry farming technology and discloses a method for reducing antibiotic use in chicken farming. This invention solves the problems of poor hygiene in the farming environment, high chicken disease rates, and the need for excessive antibiotic feeding. The method employs a small, frequent feeding approach, adding astragalus polysaccharide, phytase, fish meal, and meat and bone meal to the feed to enhance the chicken's immune function and reduce disease occurrence during the rearing process. Simultaneously, during feeding, the bottom plate is lowered, and an electric telescopic rod A and scraper are used to clean the manure on the baffle. Water is sprayed and air is blown onto the wire mesh frame during movement to remove impurities. When the feed reaches the bottom, a connecting pipe is used to change the water flow direction to clean the scraper. During ventilation, a reciprocating cleaning brush is used to remove down adhering to the mesh, preventing pathogens carried by the down from causing disease. Ultimately, this method ensures hygiene in the farming environment and reduces antibiotic use.

The invention belongs to the technical field of PCR detection, and particularly relates to a composition for detecting pathogenic bacteria, a kit, application and a detection method. The pathogenic bacteria are any one or more of enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri pathogens, and the composition comprises any one or more of a component A, a component B, a component C, a component D, a component E and a component F; the composition comprises forward and reverse primers and probes aiming at enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri. The composition provided by the invention can simultaneously detect and distinguish enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri, has high sensitivity, specificity and precision, is helpful to take treatment measures in time and customize personalized treatment schemes, reduces unnecessary use of antibiotics, and has a good application prospect. The generation of drug resistance is reduced.

The invention discloses a dairy cow digestive tract and respiratory tract disease therapeutic agent based on compound probiotics and plant extracts, through the synergistic effect of multiple strains and the multiple effects of the plant extracts, the therapeutic effect is remarkably improved, and the use of antibiotics is reduced. The preparation has the advantages of greenness, safety, remarkable curative effect and the like, and is suitable for large-scale popularization and application.

The invention relates to a method for treating heavy metals and antibiotics by using an attapulgite / carbon composite material and peracetic acid, which comprises the following steps: adding the attapulgite / carbon composite material and peracetic acid into polluted wastewater, and reacting for 6-360 minutes; the polluted wastewater comprises heavy metals and antibiotics. The preparation method of the attapulgite / carbon composite material comprises the following steps: mixing lignin and attapulgite to obtain a mixture; and calcining the mixture in an inert atmosphere to obtain the attapulgite / carbon composite material. According to the invention, the attapulgite / carbon composite material is cooperated with peracetic acid to treat heavy metals and antibiotics, so that the heavy metals can be efficiently absorbed and the antibiotics can be degraded, the problem of water acidification caused by a traditional acidic oxidant can be effectively overcome, and the pH value of the treated wastewater can be regulated and controlled in a neutral range; moreover, the technical problem of heavy metal overflow caused by addition of an acidic oxidant can be successfully overcome while the oxidation efficiency of the peracetic acid is maintained, and 1 + 1gt is realized; and 2, the synergistic effect is achieved.

The invention relates to the technical field of animal husbandry, in particular to a multipurpose animal feed conditioner and a using method thereof, and the conditioner is prepared from the following components in percentage by weight: 0.05%-0.1% of bacillus, 0.05%-0.1% of clostridium butyricum, 0.1%-0.2% of saccharomycetes, 0.05%-0.1% of lactobacillus acidophilus, 2.0% of glucose, 0.15% of yeast extract, 0.1%-0.15% of dipotassium phosphate, 0.05% of magnesium sulfate, 0.1% of sodium chloride and the balance of water. Through the synergistic effect of multiple bacteria, the feed utilization rate is remarkably increased, the animal growth is promoted, the immunity is enhanced, the use of antibiotics is reduced, the environmental pollution is reduced, and the synergistic development of economic benefits, ecological benefits and social benefits of livestock and poultry breeding is realized.

The invention relates to the technical field, in particular to a method for improving nutrient absorption efficiency of juvenile mandarin fish, which comprises the following steps: selecting healthy juvenile mandarin fish with average body mass of 30 + / -1g, body length of 11 + / -1cm and no vaccine injection and antibiotic use records, temporarily culturing the juvenile mandarin fish in an efficient circulating water culture system for 7 days, the method comprises the following steps: feeding mandarin fish with basic feed during temporary rearing, carrying out hydrogen filling treatment on a culture water body for 1 hour, 2 hours or 3 hours after 2 hours of feeding the feed at 6: 00 every day, namely 8: 00 every day, so that the hydrogen concentration in the water body reaches 200-320 ppb, and monitoring a water quality index at the same time. The deposition rate and the absorption efficiency of the juvenile mandarin fish on nutrient substances such as protein and fat are remarkably improved, an effective technical scheme is provided for solving the problem of nutrient absorption in feed mandarin fish culture, and remarkable economic benefits and ecological values are achieved.

A method of treatment for upper respiratory infections, where the treatment does not include use of antibiotic(s). Use of a nanoparticle delivery system and dose schedule can support the immune system, without the use of antibiotic(s). The nanoparticle delivery system includes Echinacea purpurea, zinc, and vitamin C. The nanoparticle delivery system can also include vitamin D.

The invention relates to a peptide that can be used as an alternative to existing antibiotics for the inhibition of pathogenic microorganisms and the treatment of infections due to these microorganisms, contributing to the prevention of resistance development arising from antibiotic use by reducing antibiotic use, is safer compared to human-derived natural peptides, has a very low toxicity compared to existing antimicrobial peptides, and also reducing tissue damage and side effects during infection by reducing inflammation due to microbial infections, also having the potential to regulate the immune system and immunomodulation, which will contribute to fight infections by influencing the activation and function of immune cells.

The present invention discloses a sustained-release levofloxacin punctal plug and a preparation method thereof. The sustained-release levofloxacin punctal plug prepared by cross-linking levofloxacin, polyglycolide, chitosan, and polylysine and then plasma-treating the plug can reduce the frequency of antibiotic use or eliminate the need for antibiotics. The plug has good patient compliance, comfort, biocompatibility, short-term degradation, minimal side effects, good water swelling properties, and is not easily dislodged.

This invention belongs to the field of environmental engineering materials technology and discloses a method for preparing an antibiotic-encapsulated nanofiber membrane biocarrier for the domestication of specific microbial populations. The aim is to achieve the domestication and control of specific microbial populations through the combination of antibiotic encapsulation and nanofiber membranes. This antibiotic-encapsulated nanofiber membrane biocarrier utilizes the high specific surface area and good permeability of the porous, expanded structure of emulsion electrospun nanofiber membranes to effectively immobilize antibiotics and achieve precise regulation of microbial populations through a slow release mechanism. Simultaneously, the microstructure of the nanofiber membrane provides a suitable growth environment for microorganisms, promoting the proliferation and domestication of specific populations. This invention not only improves the efficiency of microbial population domestication but also reduces the amount of antibiotics used, resulting in both environmental and economic benefits. The preparation process of this carrier is simple and convenient, suitable for wastewater treatment, bio-fermentation, and other fields, and has broad application prospects.

The application provides a double-strand DNA vaccine for late Edwardsiella tarda disease, which can significantly induce Th1 type immune bias, enhance fish cell immune response, improve the resistance to intracellular bacterial infection, and has the advantages of safety and high efficiency, easy preparation, strong adaptability for on-site promotion and the like. The double-strand DNA vaccine uses a fusion fragment of an immune adjuvant IL-12, which is constructed by connecting a nucleic acid fragment coding a subunit 12p35 with an amino acid sequence of SEQ ID NO:1 and a nucleic acid fragment coding a subunit 12p40 with an amino acid sequence of SEQ ID NO:3 through a flexible connecting peptide linker. The double-strand DNA vaccine realizes the synergistic expression of OmpC antigen and IL-12 adjuvant, enhances immunogenicity, and solves the problem of insufficient immune effect of a traditional DNA vaccine; and the vaccine has no obvious toxic side effect on fish bodies, is not dependent on the use of antibiotics, is green and environmentally friendly, and has a good industrialization prospect.

Compositions for identifying a pathogenic bacterial infection include a mannitol compound with one or more substituents including radioisotopes. These radiopharmaceuticals, such as a positron-emitting mannitol analogue, [18F]fluoromannitol ([18F]FMtl), are rapidly taken up by gram-positive and gram-negative bacteria such as E. coli, S. aureus, A. baumannii, S. epidermis, P. mirabilis, S. enterica, K. pneumonia, E. faecium, E. cloacae, and M. marinum, but not non-active infection sites such as sterile inflammatory sites, cancer sites, etc. Administration of these radiopharmaceuticals to and subsequent imaging of patients, e.g., via positron emission tomography (PET), enables detection of deep-seated and difficult to manage bacterial infections, such as osteomyelitis and prosthetic joint infection, or in patients with sickle cell disease. [18F]FMtl injection detects and differentiates infection rapidly. The radiolabeled mannitol compounds can be produced via nucleophilic substitution reactions that are deployable on commercially available synthesizers, facilitating straightforward and wide accessibility, and counteracting unnecessary antibiotic use.