70 results about "Antibiotic use" patented technology

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention discloses a breeding method for improving pork quality by using Chinese herbal medicine fermentation broth, and relates to the field of livestock and poultry breeding, the method comprises the following steps: preparing composite Chinese herbal medicine fermentation broth, and adding the fermentation broth into daily ration in proportion for feeding in the growth and fattening stage of live pigs, the compound Chinese herbal medicine fermentation liquor is prepared by fermenting a Chinese herbal medicine composition containing astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn through a compound microbial agent; according to the breeding method for improving the pork quality by using the Chinese herbal medicine fermentation liquor, Chinese herbal medicines such as astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn fruits are fermented through a compound microbial agent, the deep synergistic effect of the Chinese herbal medicines and probiotics is achieved, the bioavailability of effective components is remarkably improved, and therefore products better meeting high-end market requirements are produced; meanwhile, probiotics and metabolites in the fermentation liquor can effectively optimize the intestinal health of livestock and reduce the morbidity, and a reliable path is provided for reducing or replacing antibiotics.

The present application relates to the field of biological glass, in particular to a kind of composite biological glass and its preparation method and application.The present application uses rhBMP-2, doped antibacterial ion 45S5 bioactive glass and polyethylene glycol-lactide-glycolide polymer compound preparation slow-release antibacterial biological glass, the rhBMP-2 can occur synergistic effect with doped antibacterial ion 45S5 bioactive glass, compared with homologous growth factor can play more optimal synergy with antibacterial ion, so as to have higher antibacterial property and osteogenesis, while having longer slow-release effect in polyethylene glycol-lactide-glycolide polymer.The composite biological glass provided by the present application overcomes the pain points of the prior art for the treatment of oral bone defect, improves the treatment effect, reduces the amount of antibiotic use, and reduces the risk of infection.

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

This invention belongs to the field of microbial technology and discloses a strain of *Citrobacter freundii* bacteriophage PRF337, its bacteriophage composition, and its applications. The *Citrobacter freundii* bacteriophage PRF337 was deposited on May 26, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46489. The bacteriophage PRF337 exhibits broad-spectrum bactericidal activity against *Citrobacter freundii*, capable of lysing *Citrobacter freundii* strains containing different virulence genes and from different sources. It also possesses certain temperature and pH stability and can be used as an active ingredient in pharmaceutical preparations, environmental disinfectants, and water purifiers. It can effectively control *Citrobacter freundii* infection in aquatic animals such as fish and shrimp in aquaculture environments and can also be used for the purification of pathogens in aquatic environments. It is safe to use and effectively avoids the problems of antibiotic residues and pathogen resistance caused by antibiotic use.

The invention discloses a method for removing antibiotics by activating permanganate through ball milling MoS2, and belongs to the field of water treatment technologies and environmental functional materials. The method comprises the following steps: co-precipitating to synthesize MgAl-layered double hydroxides, and carrying out hydrothermal compounding and ball-milling bonding to obtain the ball-milling modified composite material and the water treatment application thereof. In order to solve the technical problems that in an existing permanganate activation technology, active species in a homogeneous system are prone to inactivation, the activation efficiency of a heterogeneous system is low, and only physical contact exists between materials, so that an interface electron transfer potential barrier is high, sodium thiosulfate is adopted for assisting ball milling to induce chemical bonding, a continuous electron transfer network is constructed, an electron-rich state is formed on the surface of MoS2, and the MoS2 is prepared. Antibiotics are synergistically oxidized through a high-valence manganese intermediate and a free radical double path, and meanwhile heavy metal is adsorbed through multiple mechanisms of sulfur coordination, sulfur vacancy anchoring and hydroxyl complexing. Under the optimal condition, the method realizes that the SMX removal rate reaches 96.8% and the lead ion removal rate reaches 99.2%.

This application provides a method and system for predicting urinary tract infections related to urodynamic examinations. It addresses the limitations of current preventative measures, where methods with high evidence levels and good efficacy, including antibiotic intravesical instillation and weekly oral cyclic antibiotic therapy, inevitably require antibiotic use. The method includes: acquiring urodynamic examination data from a target patient; analyzing the curve information in the urodynamic examination data to obtain a curve numerical sequence corresponding to a time axis; extracting a urodynamic feature set based on the curve numerical sequence; constructing a medical history feature set based on the target patient's medical history data; inputting the urodynamic feature set and the medical history feature set into a urinary tract infection risk prediction model to output a risk prediction result for urinary tract infection after urodynamic examination. The risk prediction result includes at least a risk score or risk stratification result.

This invention provides the application of Erythrina bark in the preparation of drugs for the prevention and / or treatment of infectious coryza in chickens. The active ingredient of this drug can be composed solely of Erythrina bark, making the formulation easy and convenient to implement. It can be used as a substitute for antibiotics during the egg-laying period of chickens, with no drug residue requirements, reducing the economic loss of discarded eggs after the use of antibiotics in laying hens. It has a high cure rate for infectious coryza, is safe and has no toxic side effects, and is easy to use.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.

This invention relates to the field of poultry farming technology and discloses a method for reducing antibiotic use in chicken farming. This invention solves the problems of poor hygiene in the farming environment, high chicken disease rates, and the need for excessive antibiotic feeding. The method employs a small, frequent feeding approach, adding astragalus polysaccharide, phytase, fish meal, and meat and bone meal to the feed to enhance the chicken's immune function and reduce disease occurrence during the rearing process. Simultaneously, during feeding, the bottom plate is lowered, and an electric telescopic rod A and scraper are used to clean the manure on the baffle. Water is sprayed and air is blown onto the wire mesh frame during movement to remove impurities. When the feed reaches the bottom, a connecting pipe is used to change the water flow direction to clean the scraper. During ventilation, a reciprocating cleaning brush is used to remove down adhering to the mesh, preventing pathogens carried by the down from causing disease. Ultimately, this method ensures hygiene in the farming environment and reduces antibiotic use.

The invention discloses a dairy cow digestive tract and respiratory tract disease therapeutic agent based on compound probiotics and plant extracts, through the synergistic effect of multiple strains and the multiple effects of the plant extracts, the therapeutic effect is remarkably improved, and the use of antibiotics is reduced. The preparation has the advantages of greenness, safety, remarkable curative effect and the like, and is suitable for large-scale popularization and application.

The invention relates to a method for treating heavy metals and antibiotics by using an attapulgite / carbon composite material and peracetic acid, which comprises the following steps: adding the attapulgite / carbon composite material and peracetic acid into polluted wastewater, and reacting for 6-360 minutes; the polluted wastewater comprises heavy metals and antibiotics. The preparation method of the attapulgite / carbon composite material comprises the following steps: mixing lignin and attapulgite to obtain a mixture; and calcining the mixture in an inert atmosphere to obtain the attapulgite / carbon composite material. According to the invention, the attapulgite / carbon composite material is cooperated with peracetic acid to treat heavy metals and antibiotics, so that the heavy metals can be efficiently absorbed and the antibiotics can be degraded, the problem of water acidification caused by a traditional acidic oxidant can be effectively overcome, and the pH value of the treated wastewater can be regulated and controlled in a neutral range; moreover, the technical problem of heavy metal overflow caused by addition of an acidic oxidant can be successfully overcome while the oxidation efficiency of the peracetic acid is maintained, and 1 + 1gt is realized; and 2, the synergistic effect is achieved.

The invention relates to the technical field of animal husbandry, in particular to a multipurpose animal feed conditioner and a using method thereof, and the conditioner is prepared from the following components in percentage by weight: 0.05%-0.1% of bacillus, 0.05%-0.1% of clostridium butyricum, 0.1%-0.2% of saccharomycetes, 0.05%-0.1% of lactobacillus acidophilus, 2.0% of glucose, 0.15% of yeast extract, 0.1%-0.15% of dipotassium phosphate, 0.05% of magnesium sulfate, 0.1% of sodium chloride and the balance of water. Through the synergistic effect of multiple bacteria, the feed utilization rate is remarkably increased, the animal growth is promoted, the immunity is enhanced, the use of antibiotics is reduced, the environmental pollution is reduced, and the synergistic development of economic benefits, ecological benefits and social benefits of livestock and poultry breeding is realized.

The invention relates to the technical field, in particular to a method for improving nutrient absorption efficiency of juvenile mandarin fish, which comprises the following steps: selecting healthy juvenile mandarin fish with average body mass of 30 + / -1g, body length of 11 + / -1cm and no vaccine injection and antibiotic use records, temporarily culturing the juvenile mandarin fish in an efficient circulating water culture system for 7 days, the method comprises the following steps: feeding mandarin fish with basic feed during temporary rearing, carrying out hydrogen filling treatment on a culture water body for 1 hour, 2 hours or 3 hours after 2 hours of feeding the feed at 6: 00 every day, namely 8: 00 every day, so that the hydrogen concentration in the water body reaches 200-320 ppb, and monitoring a water quality index at the same time. The deposition rate and the absorption efficiency of the juvenile mandarin fish on nutrient substances such as protein and fat are remarkably improved, an effective technical scheme is provided for solving the problem of nutrient absorption in feed mandarin fish culture, and remarkable economic benefits and ecological values are achieved.

The invention relates to a peptide that can be used as an alternative to existing antibiotics for the inhibition of pathogenic microorganisms and the treatment of infections due to these microorganisms, contributing to the prevention of resistance development arising from antibiotic use by reducing antibiotic use, is safer compared to human-derived natural peptides, has a very low toxicity compared to existing antimicrobial peptides, and also reducing tissue damage and side effects during infection by reducing inflammation due to microbial infections, also having the potential to regulate the immune system and immunomodulation, which will contribute to fight infections by influencing the activation and function of immune cells.

This invention belongs to the field of environmental engineering materials technology and discloses a method for preparing an antibiotic-encapsulated nanofiber membrane biocarrier for the domestication of specific microbial populations. The aim is to achieve the domestication and control of specific microbial populations through the combination of antibiotic encapsulation and nanofiber membranes. This antibiotic-encapsulated nanofiber membrane biocarrier utilizes the high specific surface area and good permeability of the porous, expanded structure of emulsion electrospun nanofiber membranes to effectively immobilize antibiotics and achieve precise regulation of microbial populations through a slow release mechanism. Simultaneously, the microstructure of the nanofiber membrane provides a suitable growth environment for microorganisms, promoting the proliferation and domestication of specific populations. This invention not only improves the efficiency of microbial population domestication but also reduces the amount of antibiotics used, resulting in both environmental and economic benefits. The preparation process of this carrier is simple and convenient, suitable for wastewater treatment, bio-fermentation, and other fields, and has broad application prospects.

The application provides a double-strand DNA vaccine for late Edwardsiella tarda disease, which can significantly induce Th1 type immune bias, enhance fish cell immune response, improve the resistance to intracellular bacterial infection, and has the advantages of safety and high efficiency, easy preparation, strong adaptability for on-site promotion and the like. The double-strand DNA vaccine uses a fusion fragment of an immune adjuvant IL-12, which is constructed by connecting a nucleic acid fragment coding a subunit 12p35 with an amino acid sequence of SEQ ID NO:1 and a nucleic acid fragment coding a subunit 12p40 with an amino acid sequence of SEQ ID NO:3 through a flexible connecting peptide linker. The double-strand DNA vaccine realizes the synergistic expression of OmpC antigen and IL-12 adjuvant, enhances immunogenicity, and solves the problem of insufficient immune effect of a traditional DNA vaccine; and the vaccine has no obvious toxic side effect on fish bodies, is not dependent on the use of antibiotics, is green and environmentally friendly, and has a good industrialization prospect.

Compositions for identifying a pathogenic bacterial infection include a mannitol compound with one or more substituents including radioisotopes. These radiopharmaceuticals, such as a positron-emitting mannitol analogue, [18F]fluoromannitol ([18F]FMtl), are rapidly taken up by gram-positive and gram-negative bacteria such as E. coli, S. aureus, A. baumannii, S. epidermis, P. mirabilis, S. enterica, K. pneumonia, E. faecium, E. cloacae, and M. marinum, but not non-active infection sites such as sterile inflammatory sites, cancer sites, etc. Administration of these radiopharmaceuticals to and subsequent imaging of patients, e.g., via positron emission tomography (PET), enables detection of deep-seated and difficult to manage bacterial infections, such as osteomyelitis and prosthetic joint infection, or in patients with sickle cell disease. [18F]FMtl injection detects and differentiates infection rapidly. The radiolabeled mannitol compounds can be produced via nucleophilic substitution reactions that are deployable on commercially available synthesizers, facilitating straightforward and wide accessibility, and counteracting unnecessary antibiotic use.

The invention discloses a porcine pseudorabies GE purification scheme, and the purification method comprises the following steps: S1, pig farm environment background evaluation and odor purification device installation; s2, evaluating the health status of the swinery and optimizing an immune program; s3, epidemic disease purification and odor cooperative control; s4, environment-epidemic disease linkage monitoring and early warning are carried out; and S5, maintaining the purification effect. The environment stress is reduced through the odor purification device, the immunity of pigs is enhanced, and the propagation opportunity of PRV is reduced; biodegradation and photocatalysis technologies are combined, so that a remarkable inactivation effect on PRV aerosol is achieved; establishing a first machine learning-based environment parameter and PRV propagation risk prediction model to realize early warning; the use amount of disinfectants is reduced by 40%, the use amount of antibiotics is reduced, and green breeding is achieved; the PRV purification period can be shortened, the maintenance cost is reduced, and meanwhile, the working environment of a pig farm is remarkably improved.

The invention relates to the technical field of microorganisms, and discloses an extreme Pseudomonas orientalis bacteriophage and application thereof, and the preservation number of the extreme Pseudomonas orientalis bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No.45884. The invention provides a new bacteriophage, the bacteriophage has a very strong splitting action on extreme pseudomonas orientalis, can be used for solving diseases caused by the extreme pseudomonas orientalis in corn disease prevention and control and reducing the use of antibiotics and the appearance of drug-resistant bacteria, is expected to become a new prevention and control strategy, and has a good application prospect.

The application discloses application of relative changes of CD45 molecule expression of monocytes and lymphocytes in diagnosis of infection and inflammation, and creatively finds for the first time that the index of relative changes of CD45 molecule expression of monocytes and lymphocytes can be used for quickly and accurately judging whether a patient is accompanied by infection, and effectively distinguishing whether it is viral infection or bacterial infection, and has high diagnostic efficiency, high AUC value, sensitivity and specificity, can be used for guiding clinical treatment decision and antibiotic use, and is used in early screening, prevention, treatment and post-treatment monitoring of infection, and has important transformation significance.

The invention relates to the technical field of fermentation engineering, and in particular relates to Enshi L77 for producing polyether antibiotics and application of the Enshi L77. The preservation number of the Ennuli L77 is CGMCC (China General Microbiological Culture Collection Center) No.36394. The The application comprises the following steps: fermenting by adopting the Enshi L77, extracting and separating from a fermentation product and thalli to obtain a polyether compound, and using the polyether compound as an antibiotic. According to the invention, a strain of Enshi L77 is obtained through research and screening, functional verification of the Enshi L77 shows that a polyether compound with relatively good antibacterial activity can be separated from a fermentation product of the Enshi L77, and the Enshi L77 can be used for preparing human or livestock anti-infection drugs and has a relatively good application prospect.

This invention provides the application of anti-glycolytic drugs in the preparation of drugs and / or formulations that enhance the anti-infective efficacy of anti-stress products, belonging to the field of veterinary drug technology. Experiments have shown that anti-glycolytic drugs can enhance the anti-infective efficacy of anti-stress products. This invention combines anti-glycolytic drugs with anti-stress products in a specific ratio, significantly enhancing the anti-infective ability of animals under stress, with an effect no less than that of anti-stress products combined with antibiotics, and can replace antibiotics. The method for enhancing the anti-infective efficacy of anti-stress products provided by this invention is simple, environmentally friendly, and bacteria are less likely to develop drug resistance, showing good application prospects.

This invention relates to a phage lysin LysSPYS1, a composition containing the phage lysin, and their applications. The sequence of the phage lysin LysSPYS1 is shown in SEQ ID No. 1. This phage lysin exhibits highly efficient and broad-spectrum bactericidal activity; it has a lytic effect on a variety of bacteria and can be used as a bacteriostatic agent. Furthermore, combining this phage lysin with polymyxin B can exert a synergistic antibacterial effect, significantly reducing the amount of antibiotics used. The phage lysin can also be used in combination with magnolol to achieve a prominent antibacterial effect.

This invention discloses a reagent for detecting the content of aminoglycoside antibiotics and its application method. The reagent, by volume percentage, comprises 10-20% buffer solution, 8-12% anionic surfactant, 1-5% ANS-Na aqueous solution, and 60-65% ultrapure water. The detection method involves mixing the reagent with a water sample containing aminoglycoside antibiotics, reacting, and then performing fluorescence detection. The detection result is then used to calculate the content of aminoglycoside antibiotics using a standard curve. This invention utilizes the advantages of ANS-Na's high sensitivity, rapid response, and ease of operation. It forms a ternary nanoaggregate with the anionic surfactant and AGs, providing a hydrophobic environment for the small molecule fluorescent substance ANS-Na, thereby enhancing the fluorescence signal and achieving sensitive detection of AGs.

The invention provides a piglet health-care feed additive as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The health-care feed additive for the piglets is prepared from the following raw materials in parts by weight: 30-50 parts of astragalus membranaceus, 30-50 parts of myrobalan, 25-40 parts of cynomorium songaricum, 25-40 parts of rhizoma alismatis, 25-40 parts of cortex lycii radicis, 20-30 parts of roasted rhizoma atractylodis macrocephalae, 20-30 parts of spina date seeds, 20-30 parts of bunge corydalis herb, 20-30 parts of acalypha australis, 10-20 parts of turmeric, 10-20 parts of paulownia leaves, 5-15 parts of loquat leaves, 3-5 parts of folium isatidis and 3-5 parts of hawthorn through processes such as fermentation, freeze drying and the like. The feed additive has the effects of regulating intestinal flora structures of piglets, increasing the number of beneficial bacteria in intestinal tracts, reducing field planting of harmful bacteria, reducing the occurrence rate of bacterial diarrhea of the piglets and the like, can replace antibiotics for use, does not remain harmful substances in pig bodies after being used, and is beneficial to realizing green and sustainable development of piglet breeding.

The present disclosure provides a method of treating eosinophilic COPD and / or eosinophilic inflammatory responses of eosinophilic COPD in a subject in need thereof. The method comprises orally administering to the subject a therapeutically effective amount of dexpramipexole, or a pharmaceutically acceptable salt thereof. The treating eosinophilic COPD can comprise reducing the rate of COPD exacerbation, reducing a mucus plug score, reducing the rate of hospitalizations due to exacerbations, reducing the rate of oral / parenteral steroid and / or antibiotic use for treatment of COPD, changing the level of one or more biomarkers associated with eosinophilic chronic obstructive pulmonary disorder (COPD), lung function decline, or any combination thereof.

The present application belongs to the technical field of traditional Chinese medicine, and mainly relates to a traditional Chinese medicine composition for blocking bacterial vitreous body disease of shrimplets, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: chebula 20-60 parts, schisandra 10-40 parts, and cyperus 10-40 parts. The traditional Chinese medicine composition can significantly reduce the mortality of shrimplets suffering from bacterial vitreous body disease (BVS), reduce the number of cultivable bacteria and the number of vibrio in the hepatopancreas of prawns, and has a good repair effect on the damage of the hepatopancreas and intestinal tract of the diseased shrimplets. The traditional Chinese medicine composition is simple and convenient to use, safe and green, can effectively reduce or replace the use of antibiotics, and is suitable for the control of vibrio diseases in the process of shrimplet cultivation and breeding production.