81 results about "Citrobacter freundii" patented technology

The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.

The invention provides a method for treating bacterial infections. In one aspect, the invention comprises orally administering a pharmaceutical composition to an animal, wherein the composition comprises a pharmaceutically acceptable excipient and an antibacterial effective amount of negamycin, or a pharmaceutically acceptable salt, prodrug or isomer thereof. An aspect of the invention also relates to a method of treating a bacterial infection, wherein the method comprises intravenously administering a pharmaceutical composition to an animal, and wherein the composition comprises a pharmaceutically acceptable excipient and an antibacterial effective amount of deoxynegamycin, or a pharmaceutically acceptable salt, prodrug or isomer thereof. An aspect of the invention also relates to a method of treating a bacterial infection, wherein the method comprises administering to an animal an antibacterial effective amount of negamycin or deoxynegamycin, or a pharmaceutically acceptable salt, prodrug or isomer thereof, and wherein the infecting bacteria are selected from a group of bacteria consisting of the following: Acinetobacter baumanii, Citrobacter freundii, Enterobacter aerogenes, haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus MRSA, Staphylococcus aureus GISA, Staphylococcus epidermis, Streptococcus pneumoniae PenR, Streptococcus pneumoniae PenS and Streptococcus pyogenes.

The invention discloses a Citrobacter freundii with transformed phosphorus accumulating genes. Polyphosphate kinase Ppk1 genes derived from the Citrobacter freundii are led into the Citrobacter freundii. The DNA of the Citrobacter freundii only contains Ppk1 genes, and the control way of the Ppk1 genes and Ppx genes is dual-cis-trans cotranscription. The phosphorus accumulation ability of bacteria with the characteristics can be improved by means of the Ppk1 with host bacteria as a receptor to express the source. The invention further discloses a construction method of the Citrobacter freundii with the transformed phosphorus accumulating genes and the application of the Citrobacter freundii with the transformed phosphorus accumulating genes in waste water dephosphorization. The Citrobacter freundii with the transformed phosphorus accumulating genes has the advantage of being high in dephosphorization ability.

The present invention relates to genes for detecting the genus <HIL><PDAT>Pectinatus frisingensis < / ITALIC><PDAT>or <HIL><PDAT>Pectinatus cerevisiiphilus < / ITALIC><PDAT>of the genus Pectinatus, which is known as beer-spoilage bacteria, and a method for detecting the bacteria by using the genes.< / PTEXT>The present invention provides gene sequences of spacer regions between 16S rRNA genes and 23S rRNA genes specific for the genus Pectinatus relating to beer-spoilage and a method for quickly and sensitively detecting the bacteria by using the sequences.< / PTEXT>

The invention provides a multiplex PCR (Polymerase Chain Reaction) primer for simultaneously detecting salmonella, citrobacter, proteus and Edwardsiellas and a design method thereof, and relates to the detection of salmonellae and the like. A PCR amplified fragment corresponding to a salmonella primer is 526bp, a PCR amplified fragment corresponding to a citrobacter freundii primer is 161bp, a PCR amplified fragment corresponding to a proteus mirabilis primer is 396bp and a PCR amplified fragment corresponding to an edwardsiella tarda primer is 330bp. The design method comprises the following steps of: 1, designing a multiplex PCR primer combination; 2, screening primers in the primer combination and reserving the primers which can not be used for synthesizing primer dimers; 3, judging competitive advantageous and disadvantageous states of the reserved primers and comparing GC% of the primers which are reserved in the step 2, and can not be used for synthesizing the primer dimers with a base number; selecting and judging the primers with the high GC content as the primers with the advantageous competitive states and performing step 5, and otherwise, performing step 4; step 4, screening the primers with the disadvantageous competitive states again; and 5, amplifying the primers with the advantageous competitive states.