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Electricity-producing bacterium capable of degrading cellulose and application of electricity producing bacterium in fuel cells

A technology for degrading cellulose and fuel cells, applied in biochemical fuel cells, bacteria, final product manufacturing, etc., can solve the problems of poor electricity generation performance and inability to use cellulose to generate electricity, and achieve high electricity generation voltage and high use value , easy to cultivate effect

Active Publication Date: 2015-05-13
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of electrogenic bacteria that degrades cellulose and its application in fuel cells, in order to provide a new strain selection for the industry to use cellulose to generate electricity, and also to provide a new way for the industry to use cellulose to generate electricity. Provide a new application method, and at the same time solve the problem that the strains used in the existing microbial batteries cannot use cellulose to generate electricity or have poor electricity generation performance

Method used

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  • Electricity-producing bacterium capable of degrading cellulose and application of electricity producing bacterium in fuel cells
  • Electricity-producing bacterium capable of degrading cellulose and application of electricity producing bacterium in fuel cells
  • Electricity-producing bacterium capable of degrading cellulose and application of electricity producing bacterium in fuel cells

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Embodiment 1

[0025] The acquisition of embodiment 1 bacterial strain

[0026] (1) Isolation and purification of bacterial strains: the source comes from the anode of bipolar chamber circulation MFC fueled by carboxymethyl cellulose which has been running stably for 6 months, cut a small amount of carbon cloth, and put it into the concentration of mass percent with glass beads 1% NaCl solution, shake evenly, apply gradient dilution on a plate with carboxymethyl cellulose as the sole carbon source, and place in an anaerobic box (YQX-Ⅱ, Shanghai Xinmiao Medical Instrument Manufacturing Co., Ltd.) Cultivate at 30°C for 3 days; continue to streak and separate, and store;

[0027] (2) Screening of strains: inoculate the tested strains preserved in step (1) into the enzyme-producing medium for 48 h at 37°C with shaking, take 10 mL of fermentation broth, centrifuge at 4000 r / min for 10 min, and use the supernatant as crude enzyme solution; pipette 1.5 mL of acetic acid buffer solution (0.05 mol / L...

Embodiment 2

[0033] Identification of embodiment 2 strains

[0034] Morphological identification, physiological and biochemical identification and molecular identification were carried out on the obtained Citrobacter freundii HBUZL-1 respectively. The experimental process and results are as follows.

[0035] (1) Morphological identification of strains

[0036] The bacterial strains were identified by referring to the "Usual Identification Methods for General Bacteria", and the morphology of the bacteria was observed under a microscope by Gram staining. The results show that after cultivation, some single colonies that can produce transparent circles on the plate with carboxymethyl cellulose as the only carbon source are obtained. The results of the Congo red experiment are shown in figure 2 . Known by the strain morphological identification after cultivation: the cellulose-degrading Citrobacter freundii provided by the present invention ( Citrobacter freundii ) HBUZL-1 is a gram-negati...

Embodiment 3

[0044] Example 3 Application of electrogenic bacteria in biofuel cells

[0045] Construct a microbial fuel cell (MFC) system according to the existing method. The system includes a cathode chamber, an anode chamber, a resistance box and a proton exchange membrane. ZX21, 0-100000Ω), a piece of proton exchange membrane (Ultrex CMI7000, MI Mem. Int. Inc.) was connected between the two chambers through a short side tube.

[0046] (1) Detection of electrical activity of Citrobacter freundii HBUZL-1

[0047] After the MFC reactor and anolyte were sterilized at 121°C for 20 minutes, the anode chamber was inoculated with Citrobacter freundii HBUZL-1, and the operation was started with a data acquisition system (DAM-3058R voltage acquisition module, Beijing Altai Technology Co., Ltd.), Record the voltage once every 1 minute. When the output voltage drops below 0.3V, replace the anode fuel, and the temperature is room temperature (about 25°C). After stable operation, examine its power...

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Abstract

The invention discloses an electricity-producing bacterium capable of degrading cellulose and an application of the electricity-producing bacterium in fuel cells. The strain of the electricity-producing bacteria is collected as citrobacter freundii HBUZL-1 in CGMCC with the collection number of CGMCC No.10335 on January 9, 2015; the 16SrDNA gene sequence of the strain is SEQ ID NO: 1. The electricity-producing bacteria are easy to culture; a plurality of organic matters can be taken as a substrate, and more importantly, the rich cellulose can be taken as the substrate for generating electricity; if being applied to the microbial fuel cells, the electricity-producing bacteria are high in adaptability and high in electricity-producing voltage, and have relatively high economic value and wide application prospect.

Description

technical field [0001] The invention relates to the technical field of microbial fuel cells, in particular to an electrogenic bacteria degrading cellulose and its application in fuel cells. Background technique [0002] Microbial fuel cell (MFC) is a device that uses microorganisms as the main body of the reaction, uses enzymes or microorganisms as the anode catalyst, and oxidizes organic matter into electrical energy through its metabolism. It belongs to the biochemical conversion technology in biomass energy utilization technology. It is not only pollution-free, high-efficiency, mild reaction conditions, but also has a wide range of fuel sources. Therefore, microbial fuel cells have become a subject of research all over the world. [0003] my country is a large agricultural country with abundant biomass resources. Various crops produce more than 600 million tons of straw every year, of which about 400 million tons can be used as energy. If this can be used as a substrate ...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCC12N1/205C12R2001/01H01M8/16Y02E60/50Y02P70/50
Inventor 赵丽坤李景晨李红梅
Owner HEBEI UNIVERSITY
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