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Citrobacter freundii and application thereof to production of bioelectricity

A technology of Citrobacter Citrobacter and ferric citrate is applied in the fields of environmental pollution biological treatment and bioenergy, which can solve the problem that the electric power generation characteristics of electron donors need to be proved, and achieve the effect of reducing requirements and strong electrochemical activity.

Active Publication Date: 2013-07-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the above reports, it can be seen that so far, there is no detailed report on the isolation of Citrobacter freundii electricity-producing bacteria directly from the enriched MFC anode biofilm, and the characteristics of electricity production using different electron donors remain to be ascertained

Method used

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  • Citrobacter freundii and application thereof to production of bioelectricity
  • Citrobacter freundii and application thereof to production of bioelectricity
  • Citrobacter freundii and application thereof to production of bioelectricity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Screening and isolation of strain CCTCC M2012447

[0034] Take 300mL of the biochemical pond mud of the sewage treatment plant and transfer it to a 500mL beaker, add 200mL of glucose nutrient solution, stir evenly, and use NaHCO 3 Adjust the pH value of the solution to around 7.0 (6.7-7.2) and place it in an incubator at 30°C for 1 day. Measure the pH value and use NaHCO when appropriate 3 Adjust the pH of the solution to 6.7-7.2, stir it evenly, and discard the supernatant after it settles. Repeat the cultivation for 3 days.

[0035] Start the single-chamber air cathode microbial fuel cell, the MFC is a rectangular parallelepiped (length×width×height=8.5×7×6.5cm 3 , Effective volume 200mL), the material is 0.5cm plexiglass, one end is closed with a blind plate, and the other end is placed with a splint. The air cathode is platinum-loaded carbon paper (0.5mg·cm -2 ), the anode is nitric acid modified carbon felt (BET1200m 2 ·G -1 , 2mm), the electrode spacing is 3cm, and ti...

Embodiment 2

[0038] Determination of electrochemical activity of strain CCTCC M2012447

[0039] Inoculate Citrobacter freundii Z7 into LB medium and ferric citrate medium (tryptone 10g·L -1 , Yeast extract 5g·L -1 , Sodium chloride 10g·L -1 , 20mmol·L -1 Ferric citrate, pH 7.4), (30±1)℃ anaerobic culture for 18h. Take the bacterial solution in the mid-log phase of growth, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, centrifuge the resulting bacterial suspension again according to the above steps, repeat washing twice, add PBS buffer Make bacterial suspension, OD 600 Is about 0.6. The cyclic voltammetry test was performed on the obtained bacterial suspension with an electrochemical workstation. The test adopts a three-electrode system, with glassy carbon electrode as the working electrode, Ag / AgCl electrode as the reference electrode, platinum plate as the counter electrode, and the scanning speed is 100mV·s -1 , The scanning range is -0.7V~0....

Embodiment 3

[0042] Application of strain CCTCC M2012447 in the treatment of different types of artificial simulated wastewater

[0043] The strain CCTCC M2012447 was inoculated into ferric citrate medium (tryptone 10g·L -1 , Yeast extract 5g·L -1 , Sodium chloride 10g·L -1 , 20mmol·L -1 Ferric citrate, pH 7.4), (30±1)℃ anaerobic culture for 18h. Take the bacterial solution in the mid-log phase of growth, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, centrifuge the resulting bacterial suspension again according to the above steps, repeat washing twice, add PBS buffer Make bacterial suspension, OD 600 Is about 0.6.

[0044] Start the single-chamber air cathode microbial fuel cell, the microbial fuel cell is cylindrical (the cross-sectional diameter is 2cm, the effective volume is 6.28cm 3 ), the material is polycarbonate. The air cathode is platinum-loaded carbon paper (0.5mg·cm -2 ), the anode is nitric acid modified carbon felt, the inside of ...

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Abstract

The invention discloses a citrobacter freundii and an application thereof to the production of bioelectricity. The (citrobacter freundii) Z7 is collected in the China center for type culture collection short for CCTCC on November 6th 2012, wherein the collection number of the (citrobacter freundii) Z7 is CCTCC NO: M2012447. The citrobacter freundii can transfer electrons to an extracellular electron acceptor under an anaerobic condition, can be inoculated in a microbial fuel cell and can generate electric energy while degrading organisms. Compared with other strains, the (citrobacter freundii) Z7 not only has strong electrochemical activity, but also can generate electricity by taking various types of organisms as a unique carbon source. Based on the characteristics, the citrobacter freundii can be well applied to environment pollution restoration and biological energy recovery.

Description

Technical field [0001] The invention belongs to the technical field of environmental pollution biological treatment and bioenergy, and specifically relates to an electricity-producing Citrobacter fredii and its application. Background technique [0002] Since the industrial revolution, sewage treatment has always been a common concern of the whole world, especially developing countries. Sewage treatment can obtain significant social and environmental benefits. However, traditional sewage treatment processes have high energy consumption and high operating and management costs, so the input is large, the output is small, and the economic benefits are meager. In recent years, the rise of Microbial Fuel Cell (MFC) technology has provided a new way for the sustainable development of energy and environment. [0003] MFC is a special fuel cell that uses microorganisms as biocatalysts. The organic or inorganic substances in the fuel can be oxidized to carbon dioxide in the anode zone. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCY02E60/50Y02P70/50
Inventor 朱能武彭月吴平霄
Owner SOUTH CHINA UNIV OF TECH
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