Citrobacter freundii and application thereof to production of bioelectricity
A technology of Citrobacter Citrobacter and ferric citrate is applied in the fields of environmental pollution biological treatment and bioenergy, which can solve the problem that the electric power generation characteristics of electron donors need to be proved, and achieve the effect of reducing requirements and strong electrochemical activity.
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Embodiment 1
[0033] Screening and isolation of strain CCTCC M2012447
[0034] Take 300mL of the biochemical pond mud of the sewage treatment plant and transfer it to a 500mL beaker, add 200mL of glucose nutrient solution, stir evenly, and use NaHCO 3 Adjust the pH value of the solution to around 7.0 (6.7-7.2) and place it in an incubator at 30°C for 1 day. Measure the pH value and use NaHCO when appropriate 3 Adjust the pH of the solution to 6.7-7.2, stir it evenly, and discard the supernatant after it settles. Repeat the cultivation for 3 days.
[0035] Start the single-chamber air cathode microbial fuel cell, the MFC is a rectangular parallelepiped (length×width×height=8.5×7×6.5cm 3 , Effective volume 200mL), the material is 0.5cm plexiglass, one end is closed with a blind plate, and the other end is placed with a splint. The air cathode is platinum-loaded carbon paper (0.5mg·cm -2 ), the anode is nitric acid modified carbon felt (BET1200m 2 ·G -1 , 2mm), the electrode spacing is 3cm, and ti...
Embodiment 2
[0038] Determination of electrochemical activity of strain CCTCC M2012447
[0039] Inoculate Citrobacter freundii Z7 into LB medium and ferric citrate medium (tryptone 10g·L -1 , Yeast extract 5g·L -1 , Sodium chloride 10g·L -1 , 20mmol·L -1 Ferric citrate, pH 7.4), (30±1)℃ anaerobic culture for 18h. Take the bacterial solution in the mid-log phase of growth, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, centrifuge the resulting bacterial suspension again according to the above steps, repeat washing twice, add PBS buffer Make bacterial suspension, OD 600 Is about 0.6. The cyclic voltammetry test was performed on the obtained bacterial suspension with an electrochemical workstation. The test adopts a three-electrode system, with glassy carbon electrode as the working electrode, Ag / AgCl electrode as the reference electrode, platinum plate as the counter electrode, and the scanning speed is 100mV·s -1 , The scanning range is -0.7V~0....
Embodiment 3
[0042] Application of strain CCTCC M2012447 in the treatment of different types of artificial simulated wastewater
[0043] The strain CCTCC M2012447 was inoculated into ferric citrate medium (tryptone 10g·L -1 , Yeast extract 5g·L -1 , Sodium chloride 10g·L -1 , 20mmol·L -1 Ferric citrate, pH 7.4), (30±1)℃ anaerobic culture for 18h. Take the bacterial solution in the mid-log phase of growth, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, centrifuge the resulting bacterial suspension again according to the above steps, repeat washing twice, add PBS buffer Make bacterial suspension, OD 600 Is about 0.6.
[0044] Start the single-chamber air cathode microbial fuel cell, the microbial fuel cell is cylindrical (the cross-sectional diameter is 2cm, the effective volume is 6.28cm 3 ), the material is polycarbonate. The air cathode is platinum-loaded carbon paper (0.5mg·cm -2 ), the anode is nitric acid modified carbon felt, the inside of ...
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