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Citrobacter freundii with phosphorus-dissolving potassium-dissolving capability and application thereof

A technology of citric acid and ability of freundii, which is applied to Citrobacter freundii with the ability of dissolving phosphorus and potassium and its application field, to achieve the effect of simple nutritional requirements, short growth cycle and growth promotion

Active Publication Date: 2015-02-25
西安金博瑞生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the modification of microorganisms mainly includes mutagenesis and genetic engineering methods, but for microbial fertilizers, because live bacteria are directly applied to the soil and the environment, the use of genetically engineered bacteria is clearly restricted in my country's bacterial fertilizer standards. application
Radiation, nuclear radiation and chemical mutagenesis are commonly used methods of mutation breeding at present, but long-term use of a mutagen will often make strains resistant, and a new mutagenic source must be used in order to obtain greater variation

Method used

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  • Citrobacter freundii with phosphorus-dissolving potassium-dissolving capability and application thereof
  • Citrobacter freundii with phosphorus-dissolving potassium-dissolving capability and application thereof
  • Citrobacter freundii with phosphorus-dissolving potassium-dissolving capability and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] According to the screening method of the phosphorus-dissolving bacterial strain provided by the present invention, the bacterial strain with strong phosphorus-dissolving ability is selected, and the preparation steps are as follows:

[0039] 1) Use Citrobacter freundii ZSW-4 screened from the plant rhizosphere in the laboratory as the starting strain;

[0040] 2) Mutation breeding

[0041] (1) The Citrobacter freundii with phosphorus-solubilizing ability provided by the present invention is obtained through a mutagenesis selection method, which includes the following steps:

[0042] 1) Citrobacter freundii ZSW-4, which was screened from the plant rhizosphere in the laboratory, was used as the starting strain;

[0043] 2) Mutation breeding

[0044] (1) Prepare the single cell suspension of the starting strain ZSW-4

[0045] The starting strain ZSW-4 was inoculated in liquid medium A, cultured at 28°C, 180rpm for 16 hours, centrifuged, washed with sterile saline, place...

Embodiment 2

[0064] Example 2 Determination of ZSW-4-2-5C's ability to decompose calcium phosphate, lecithin and calcium phytate

[0065] Dispense 100ml of liquid culture medium B, C or D into 250ml Erlenmeyer flasks, and sterilize them for later use. Inoculate ZSW-4-2-5C in 50ml of liquid medium A, culture at 28°C for 36 hours, take 100μl (dilute A bacteria solution with liquid medium to make its OD600 0.7) and inoculate it in the above In the liquid culture medium B, C or D, each group was cultured in three parallels at 28°C and 150rpm for 7 days, and the bacteria liquid cultured on the above shaker was taken, centrifuged at 10,000rpm for 10 minutes, and the supernatant was taken and determined by molybdenum-antimony resistance method Phosphorus content in the supernatant. The experimental results show that: ZSW-4-2-5C has a calcium phosphate dissolving ability of 647.1±25.2 mg / l in liquid medium B, and a lecithin dissolving ability of 105±6.4 mg / l in liquid medium C. The ability to de...

Embodiment 3

[0070] Example 3 Determination of the ability of ZSW-4-2-5C to produce indole acetic acid (IAA)

[0071] Add sterile L-tryptophan with a concentration of 200μg·ml-1 to a 250ml Erlenmeyer flask containing 100ml of liquid medium A, inoculate ZSW-4-2-5C in 50ml of liquid medium A, at 28°C, After culturing at 150rpm for 20 hours, take 100μl (dilute the bacterial solution with medium A to make its OD600 0.7) and inoculate it into the above-mentioned liquid medium A containing L-tryptophan, set up three parallels, 28°C, 150 Rpm cultured for 72 hours, centrifuged at 4000 g for 10 min, and the concentration of IAA in the supernatant was determined by spectrophotometry. The experimental results showed that: ZSW-4-2-5C produced IAA concentration of 44.6±3.2mg / l.

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Abstract

The invention discloses citrobacter freundii with phosphorus-dissolving potassium-dissolving capability and application thereof. A bacterial strain ZSW-4 with relatively phosphorus dissolving capability is screened out from soybean rhizosphere, an ultraviolet-ray plasma composite mutation technology is utilized for performing multiple composite mutation on the bacterial strain, and a bacterial strain ZSW-4-2-5C with high dissolving efficiency on phosphorus and good stability is selected and bred, and is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 8748. By using a liquid containing the bacterium or a solid inocula to inoculate corn, soybean, mustard and other crops, absorption of plants on phosphorus element in soil is promoted and crop output is improved.

Description

technical field [0001] The invention relates to a highly efficient Citrobacter freundii ( Citrobacter fredndii ) and applications thereof, belonging to the technical field of agricultural microorganisms. technical background [0002] Phosphorus is one of the three major elements of plant nutrition, and insufficient supply of phosphorus has gradually become one of the most important factors limiting crop yield and quality. The total phosphorus content in the soil is sufficient, containing 0.02-0.5% total phosphorus, but most of them exist in the form of insoluble inorganic phosphates (mainly calcium phosphate, aluminum phosphate, iron phosphate, mainly calcium phosphate in alkaline soil) , only 1% exists in the form of soluble phosphorus salts that plants can directly absorb and utilize. In order to meet the needs of crop growth, nearly 30 million tons of phosphorus fertilizers are applied to the soil every year around the world, but 80% of them become ineffective phosphoru...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 朱晓丽宋进喜马俊杰李贺
Owner 西安金博瑞生态科技有限公司
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