Bacterial strain capable of degrading polycyclic aromatic hydrocarbons and application of bacterial strain

A technology of polycyclic aromatic hydrocarbons and bacterial strains, applied in the field of bioengineering, can solve problems such as application limitations, and achieve the effects of improving universality, good degradation ability, and rapid degradation ability

Active Publication Date: 2017-01-04
TAIZHOU VOCATIONAL & TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still relatively few bacterial strains capable of degrading polycyclic aromatic hydrocarbons, esp

Method used

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  • Bacterial strain capable of degrading polycyclic aromatic hydrocarbons and application of bacterial strain
  • Bacterial strain capable of degrading polycyclic aromatic hydrocarbons and application of bacterial strain
  • Bacterial strain capable of degrading polycyclic aromatic hydrocarbons and application of bacterial strain

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Experimental program
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Effect test

Embodiment 1

[0023] Isolation and screening of strain Citrobacter freundii L2-14 capable of degrading polycyclic aromatic hydrocarbons

[0024] Collect surface soil samples at a distance of 15cm below the surface from a solid waste dismantling site, add 1mL acclimation solution per 1g of soil sample (the ratio of soil sample: acclimatization solution is 1g: 1mL) and add acclimatization solution for acclimatization and cultivation. The culture conditions are temperature 25°C, rotation speed 100rpm, time 72h. Wherein, the formula of above-mentioned domestication liquid is:

[0025] (NH 4 ) 2 SO 4 : 2g / L; MgSO 4 : 0.2g / L; K 2 HPO 4 : 4g / L; KH 2 PO 4 : 6g / L; polycyclic aromatic hydrocarbons (naphthalene, phenanthrene, fluoranthene, pyrene, benzanthracene and benzopyrene) mixture 200mg / L, wherein, naphthalene, phenanthrene, fluoranthene, pyrene, benzanthracene and benzopyrene The addition amount can be the same amount or different amounts, mainly because the addition of these polycycli...

Embodiment 2

[0032]The Citrobacter freundii L2-14 strain was used to degrade polycyclic aromatic hydrocarbons

[0033] Take this strain Citrobacter freundii L2-14 and inoculate it in LB solid medium for activation; then select a single colony and inoculate it in 5 mL of LB liquid medium for cultivation, and cultivate it at a temperature of 28°C and a rotational speed of 100rpm until the bacterial liquid OD 600 ≈1; then, inoculate the bacterial solution in the inorganic salt glucose medium according to the inoculum amount of 2%, and cultivate it to the OD of the bacterial solution at a temperature of 28°C and a rotation speed of 100rpm 600 ≈1; Among them, the formula of inorganic salt glucose medium is: peptone: 5g / L; glucose: 10g / L; (NH4) 2 SO 4 : 2g / L; MgSO 4 : 0.2g / L; K 2 HPO 4 : 4g / L; KH 2 PO 4 : 6g / L, after the cultivation, the corresponding bacterial liquid was obtained.

[0034] Centrifuge the above bacterial solution with a centrifuge at 10,000 r / min for 5 minutes, remove the...

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Abstract

The invention relates to a bacterial strain capable of degrading polycyclic aromatic hydrocarbons and application of the bacterial strain, and belongs to the technical field of bioengineering. The novel bacterial strain aims at achieving the purpose of degrading polycyclic aromatic hydrocarbons. The bacterial strain is Citrobacter freundii L2-14, and the preservation number of the bacterial strain in China Center for Type Culture Collection is CCTCC NO:M2016371. The bacterial strain is used for remediating soil contaminated by polycyclic aromatic hydrocarbons. The bacterial strain capable of degrading polycyclic aromatic hydrocarbons has the capability of growing with polycyclic aromatic hydrocarbons as a carbon source and an energy source, can rapidly degrade polycyclic aromatic hydrocarbon contaminants and is wide in substrate degradation range and high in degradation rate.

Description

technical field [0001] The invention relates to a bacterial strain capable of degrading polycyclic aromatic hydrocarbons and an application thereof, belonging to the technical field of bioengineering. Background technique [0002] PAHs are a class of persistent organic pollutants. The pollution of polycyclic aromatic hydrocarbons in the soil environment mainly comes from the production and use of petroleum and its derivatives and the incomplete combustion of hydrocarbon-containing substances such as wood, organic polymer compounds, and paper. Due to the carcinogenicity, mutagenicity and teratogenicity of polycyclic aromatic hydrocarbons and their derivatives, the problem of PAH pollution remediation has attracted great attention. [0003] At present, for these polycyclic aromatic hydrocarbon pollutants, some specific types of microorganisms can catalyze various chemical reactions such as redox, decarboxylation, hydrolysis and dehydration of polycyclic aromatic hydrocarbons ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 张昕欣江雪青李金于红艳王玉新
Owner TAIZHOU VOCATIONAL & TECHN COLLEGE
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