Standard sample of staphylococcus aureus in milk powder and preparation method of standard sample of staphylococcus aureus in milk powder

A staphylococcus and standard sample technology, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of short storage period of samples, poor sample uniformity and stability, easy change of target bacteria, etc., to achieve The biochemical characteristics do not mutate and the effect of ensuring stability

Inactive Publication Date: 2017-10-10
CHINESE ACAD OF INSPECTION & QUARANTINE
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the target bacteria are prone to change (reproduction and death, etc.) in the prepared Staphylococcus aureus sample, it wil

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Standard sample of staphylococcus aureus in milk powder and preparation method of standard sample of staphylococcus aureus in milk powder
  • Standard sample of staphylococcus aureus in milk powder and preparation method of standard sample of staphylococcus aureus in milk powder
  • Standard sample of staphylococcus aureus in milk powder and preparation method of standard sample of staphylococcus aureus in milk powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Staphylococcus aureus standard sample in milk powder, comprises target bacterium and background flora, and described target bacterium is Staphylococcus aureus (Staphylococcus aureus), and described background flora consists of Citrobacter freundii (Citrobacterfreundii), Escherichia coli Bacillus (E.coil), Klebsiella pneumoniae (Klebsiellapnenmoniae), Bacillus cereus (Bacillus cereus).

[0028] The sample is based on trehalose, skimmed milk powder and sterile water, wherein the volume fraction of trehalose is 12%, and the volume fraction of skimmed milk powder is 15%.

[0029] The target concentration of target bacteria in the sample is 10 2 ~10 3 CFU / mL, the target concentration of background flora is 10 3 CFU / mL.

Embodiment 2

[0031] Such as figure 1 As shown, a method for preparing a standard sample of Staphylococcus aureus in milk powder in Example 1, including the selection of sample addition strains, the preparation of lyoprotectant, sample freeze-drying, sample homogeneity and stability test four Steps, the specific steps are as follows:

[0032] 1. Selection of sample addition strains

[0033] According to target bacteria: Staphylococcus aureus (Staphylococcus aureus), background flora: Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiellapnemmoniae), Bacillus cereus (Bacillus cereus), Freund's citric acid Bacillus (Citrobacterfreundii) selects standard strains, and all standard strains are purchased from institutions designated by the government, and a strain certificate is attached to ensure the traceability of the strains.

[0034] 2. Preparation of lyoprotectant

[0035] The sample was prepared with trehalose, skimmed milk powder and sterile water as the matrix (volume fraction: ...

Embodiment 3

[0072] Each step of the preparation method of the Staphylococcus aureus standard sample in milk powder described in this embodiment is all the same as in Example 2, and the different technical parameters are: in the preparation of the bacterial suspension, the concentration of the target bacteria in the target bacterial suspension Concentration according to 2×10 4 CFU / mL preparation; freeze-drying process 50h.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of quality control in microbial detection and particularly relates to a standard sample of staphylococcus aureus in milk powder and a preparationmethod of the standard sample of the staphylococcus aureus in the milk powder. The standard sample of the staphylococcus aureus in the milk powder comprises a target bacterium and background flora, wherein the target bacterium is the staphylococcus aureus; the background flora comprises Citrobacter freundii,E. coli, Klebsiella pneumonia and Bacillus cereus. The standard sample meets requirements for uniformity and stability, the stability of the standard sample can be guaranteed to the greatest extent, and a perfect preparation technology for the standard sample is formed by starting from meeting special transport conditions by means of research in special process, stability and uniformity and the like, is suitable for preparing the standard sample, meeting the requirements for uniformity and stability, of the staphylococcus aureus in the milk powder and is applicable to quality control, method validation and other purposes in a laboratory.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a standard sample of Staphylococcus aureus in milk powder and a preparation method thereof. Background technique [0002] The field of microbiological testing of milk powder is very special. At present, there is no precedent for the preparation of milk powder microbiological standard samples and large-scale production by institutions at home and abroad, which is still a blank field. At present, although some institutions prepare standard samples of microorganisms, the uniformity and stability of the standard samples can hardly meet the requirements, and transportation is extremely difficult; or the standard samples are pure strains containing only target bacteria, which are far from the actual samples Meet the needs of daily laboratory quality control. [0003] Staphylococcus aureus is a common Gram-positive bacterium that is widely distribut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/14C12N1/20C12N1/04C12R1/19C12R1/445C12R1/22C12R1/085C12R1/01
CPCC12N1/04C12N1/20C12Q1/14G01N33/56938
Inventor 赵红阳卢行安刘莉莉孙英健王秀君王伟王建华凌莉
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap