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Application of citrobacter freundii in electricity generation by microorganism and electricity generation method

A technology of Freund's citric acid and microbial power generation, which is applied in the field of environment and new energy, can solve the problems that the electrogenic activity of Citrobacter freundii has not been discovered, and achieve the effects of large electricity production voltage, easy cultivation, and strong adaptability

Inactive Publication Date: 2010-06-09
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Citrobacter freundii is mainly used in the research of clinical pathogenic bacteria and enzyme engineering. There is no report about the electrogenic activity of Citrobacter freundii at home and abroad.

Method used

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  • Application of citrobacter freundii in electricity generation by microorganism and electricity generation method
  • Application of citrobacter freundii in electricity generation by microorganism and electricity generation method
  • Application of citrobacter freundii in electricity generation by microorganism and electricity generation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the screening of bacterial strain Citrobacter freundiiLY-3 of the present invention

[0027] 1. Strain Isolation

[0028] Collect mud samples at a depth of 10cm below the surface of the dry lake bottom in South China Agricultural University (Guangzhou), and quickly transfer them to an anaerobic operation box that has reached anaerobic conditions. uniform. Take 1ml of the sample diluent and inject it into 10ml of IRM liquid medium, enrich and culture for three generations at 30°C. Then, streak on LB solid medium, culture anaerobically at 30°C for 3 days, observe the characteristics of the colonies, pick out bacteria with different colony shapes and separate them by streaking, culture for 3 generations to obtain pure strains, and store them until electrochemical investigation.

[0029] Wherein, the component of IRM medium is: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO 4 0.6g / L, NaAc 20mM, ferric citrate 20mM, yeast extract 0.5g / L.

[0030] ...

Embodiment 2

[0033] Embodiment 2: the identification of bacterial strain Citrobacter freundiiLY-3 of the present invention

[0034] The LY-3 strain obtained in Example 1 was identified by observation of morphological characteristics, physiological and biochemical assays, and 16S rDNA gene sequence analysis.

[0035] 1. Observation of Morphological Characteristics

[0036] The surface of the LY-3 strain colony is smooth, translucent, with neat edges and glossy surface.

[0037] 2. Physiological and biochemical assays

[0038] The physiological and biochemical properties of strain LY-3 are compared with those of Citrobacter species in the "Common Bacteria System Identification Manual" as shown in Table 1.

[0039] Table 1 Comparison of physiological and biochemical properties of strain LY-3 and Citrobacter freundii

[0040]

[0041]

[0042] Note: +: positive; -: negative; ND: not tested; d: different

[0043] 3.16S rDNA Phylogenetic Analysis

[0044] The whole gene of LY-3 was ex...

Embodiment 3

[0049] Example 3: Investigation of the electricity production of the strain Citrobacter freundii LY-3 of the present invention using glucose as fuel

[0050] In this example, a microbial fuel cell using LY-3 to generate electricity is constructed according to the prior art and methods. The microbial fuel cell uses two chambers, including an anode chamber and a cathode chamber, an exchange membrane and an external circuit. The electrode material is carbon felt, and the connection external resistance is 1000Ω.

[0051] The electricity generation principle of MFC is: organic matter is oxidized by Citrobacter freundii as fuel in the anaerobic anode chamber to generate electrons and protons, among which electrons are captured by Citrobacter freundii and transferred to the anode of the battery, and the electrons arrive through the external circuit The cathode, thus forming a circuit to generate current, and the protons pass through the exchange membrane to the cathode, where they re...

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Abstract

The invention discloses an application of citrobacter freundii in electricity generation by microorganism and an electricity generation method. The citrobacter freundii is taken as anode catalyst of the microbiological fuel cell to be applied to electricity generation by microorganism. The electricity generation method comprises preparing citrobacter freundii to be as inoculum for microbiological fuel cell; preparing catholyte and anolyte containing fuel; adding the inoculum for microbiological fuel cell into the anode chamber of the microbiological fuel cell, standing for culturing, and carrying out electricity generation detection. The invention opens up a new application field for citrobacter freundii and provides a new highly adaptable electrogenesis microorganism. The citrobacter freundii LY-3 is easy to culture, and the electrogenesis is high with various organism electrogenesis utilized.

Description

technical field [0001] The invention belongs to the technical field of environment and new energy, and in particular relates to the application of Citrobacter freundii in microbial power generation and a power generation method thereof. Background technique [0002] In recent years, facing the double crisis of energy shortage and environmental pollution, microbial fuel cell (Microbial Fuel Cell, MFC) has shown great research and application value due to its unique properties. A microbial fuel cell is a device that uses microorganisms as a catalyst to directly convert chemical energy in fuel into electrical energy. As early as 1911, British botanist Potter discovered that microorganisms could generate electricity. But then, the research on microbial fuel cells has been slow, until the 1980s, the wide application of redox mediators has greatly improved the output power of MFC, thus promoting the development of MFC. However, redox mediators are expensive and some are toxic, w...

Claims

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Application Information

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IPC IPC(8): H01M4/90H01M8/16C12N1/20C12R1/01
CPCY02E60/527Y02E60/50
Inventor 孙永明李颖袁振宏孔晓英李连华马隆龙吴创之
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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