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Compound biological preparation and application thereof in repairing polychlorinated biphenyls polluted soil

A biological agent and seed technology, applied in the field of microorganisms, can solve problems such as poor degradation efficiency and unreasonable compatibility of PCBs degradation bacteria agents, and achieve the effect of simple preparation method, easy method, and repair of soil pollution

Active Publication Date: 2018-01-16
江苏诚冉环境修复工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defects of unreasonable compatibility and poor degradation efficiency of PCBs degrading bacterial agents in the prior art, the present invention provides a composite biological agent

Method used

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  • Compound biological preparation and application thereof in repairing polychlorinated biphenyls polluted soil

Examples

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Effect test

Embodiment 1

[0032] A composite biological preparation prepared from five bacteria including Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus;

[0033] Specifically, the composite biological preparation is prepared according to the following steps:

[0034] 1) Add corn steep liquor 8g, glucose 6g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, calcium chloride 0.1g, ferrous sulfate 0.01g, manganese sulfate 0.01g into water, stir Evenly, adjust the pH to 7.0-7.5, and then adjust the volume to 1L to obtain a liquid fermentation medium;

[0035] 2) Activate Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus, and then culture them to the seed solution with a concentration of 30 million cfu / ml, and mix the above five The seed solution was mixed according to the volume ratio of 3:2:2:1:1, and then inoculated into the fermenter containing the li...

Embodiment 2

[0040] A composite biological preparation prepared from five bacteria including Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus;

[0041] Specifically, the composite biological preparation is prepared according to the following steps:

[0042] 1) Add corn steep liquor 8g, glucose 6g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, calcium chloride 0.1g, ferrous sulfate 0.01g, manganese sulfate 0.01g into water, stir Evenly, adjust the pH to 7.0-7.5, and then adjust the volume to 1L to obtain a liquid fermentation medium;

[0043] 2) Activate Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus, and then culture them to the seed solution with a concentration of 0.5 billion cfu / ml, and mix the above five The seed solution was mixed according to the volume ratio of 5:3:3:2:2, and then inoculated into the fermenter containing the liq...

Embodiment 3

[0048] A composite biological preparation prepared from five bacteria including Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus;

[0049] Specifically, the composite biological preparation is prepared according to the following steps:

[0050] 1) Add corn steep liquor 8g, glucose 6g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, calcium chloride 0.1g, ferrous sulfate 0.01g, manganese sulfate 0.01g into water, stir Evenly, adjust the pH to 7.0-7.5, and then adjust the volume to 1L to obtain a liquid fermentation medium;

[0051] 2) Activate Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus, and then culture them to the seed solution with a concentration of 20 million cfu / ml, and mix the above five The seed solution was mixed according to the volume ratio of 5:3:2:2:1, and then inoculated into a fermenter containing a liquid...

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Abstract

The invention belongs to the technical field of microorganisms and discloses a compound biological preparation. The compound biological preparation is prepared from rhodopseudomonas palustris, citrobacter freundii, shewanella, pseudomonas putida and bacillus pumilus. The compound biological preparation disclosed by the invention is prepared by matching five strains; all the strains promote each other, and a specific carrier is matched, so that the degradation capability on polychlorinated biphenyls is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound biological agent and its application in repairing polychlorinated biphenyl polluted soil. Background technique [0002] Polychlorinated biphenyls (polychlorinated biphenyls, PCBs alias: chlorinated biphenyls; PCBs are labeled according to the number of chlorine atoms or the percentage of chlorine. In my country, it is customary to use the number of chlorine-substituted biphenyls (regardless of their substitution positions) The PCB is divided into trichlorobiphenyl (PCB3), tetrachlorobiphenyl (PCB4), pentachlorobiphenyl (PCB5), hexachlorobiphenyl (PCB6), heptachlorobiphenyl (PCB7), octachlorobiphenyl (PCB8 ), nonachlorinated biphenyl (PCB9), decachlorinated biphenyl (PCB10). Polychlorinated biphenyls are one of the 12 persistent organic pollutants first included in the Stockholm Convention controlled list by the United Nations Environment Program (UNEP)...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/38C12R1/01C12R1/40C12R1/08
CPCC12N1/20B09C1/10Y02A50/20
Inventor 裘正荣王国平徐丹云
Owner 江苏诚冉环境修复工程有限公司
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